| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,264,883 | C→G | 6.9% | F32L (TTC→TTG) ‡ | vanB → | vanillate O‑demethylase oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,264,883 | 0 | C | G | 6.9% | 184.9 / 4.2 | 58 | F32L (TTC→TTG) ‡ | vanB | vanillate O‑demethylase oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base C (29/25); new base G (2/2); total (31/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.94e-01 | |||||||||||
AGCCGCAACTGATCGCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTA‑GTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCACG > NC_002947/4264738‑4265028 | agccgcaACTGATCGCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTA‑GTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAgc > 8:192631/1‑149 (MQ=255) gccgcaACTGATCGCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTATAGGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAgc > 1:289500/1‑149 (MQ=255) cgcaACTGATCGCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTA‑GTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCCGCCGGCGCTGAACGcc > 6:345085/1‑149 (MQ=255) caACTGATCGCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTA‑GTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCgg > 6:30056/1‑149 (MQ=255) aTCGCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTA‑GTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCCGCCGGCGCTGAACGCCGgcagca < 5:345085/148‑5 (MQ=255) gCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTA‑GTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGACCCGGCGCCCata > 1:34161/1‑148 (MQ=255) accaGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTA‑GTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGa > 4:129653/1‑149 (MQ=255) ccAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTA‑GTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCTCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCAc < 8:96460/149‑1 (MQ=255) cctgcctgAGGAACAGCCGACATGATCGATGCCGTA‑GTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCACCTGcc > 1:306545/1‑149 (MQ=255) ctgAGGAACAGCCGACATGATCGATGCCGTA‑GTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCACCTGCCCGACg > 6:123480/1‑149 (MQ=255) tgAGGAACAGCCGACATGATCGATGCCGTA‑GTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGA‑CGCGCACCAGCCCGTCgg > 4:80231/1‑149 (MQ=255) gAGGAACAGCCGACATGATCGATGCCGTA‑GTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCACCTGc < 4:220098/141‑1 (MQ=255) gAGGAACAGCCGACATGATCGATGCCGTA‑GTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCACCTGc > 3:220098/1‑141 (MQ=255) aGGAACAGCCGACATGATCGATGCCGTA‑GTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCACCTGCCCGACGGGc < 1:30306/149‑1 (MQ=255) gAACAGCCGACATGATCGATGCCGTA‑GTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCACCTGCCCGACGGGCTg > 6:80464/1‑149 (MQ=255) aCAGCCGACATGATCGATGCCGTA‑GTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGA‑CGCGCACCAGCCCGTCGGGCAGGt < 3:80231/149‑1 (MQ=255) aGCCGACATGATCGATGCCGTA‑GTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCACCTGCCCGACGGGCTGGTgc > 8:316513/1‑149 (MQ=255) ccGACATGATGGATGGCGTA‑GTGGTATCCCGTATGGATGAAGCGCAGGGTATCTGCAGCTTCGAGGTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCACCTGCCCGACGGGCTGGTgcgc < 2:34161/149‑1 (MQ=255) ccGACATGATCGATGCCGTA‑GTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCACCTGCCCGACGGGCTGGTgcgc < 7:316513/149‑1 (MQ=255) ccGACATGATCGATGCCGTA‑GTGGCATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCACCAGCCCGTCGGGCAGGTgcac < 3:129653/149‑3 (MQ=255) tCGATGCCGTA‑GTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCg > 1:251661/1‑149 (MQ=255) gATGCCGTA‑GTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGc < 2:289500/148‑1 (MQ=255) aTGCCGTA‑GTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCtg < 3:150344/149‑1 (MQ=255) aTGCCGTA‑GTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCtg > 2:251573/1‑149 (MQ=255) cGTA‑GTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACCGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGc < 1:251573/149‑1 (MQ=255) cccGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCga < 7:192631/148‑1 (MQ=255) tAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAAc > 1:253683/1‑149 (MQ=255) tGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATc < 5:92210/149‑1 (MQ=255) gcgcAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCCCCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCt > 8:286820/1‑148 (MQ=255) gcAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATc < 5:123480/149‑1 (MQ=255) cAGGGTATCTGCAGCTTCGAGCTGGCCGCGGTAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCt > 3:99438/1‑149 (MQ=255) ccgtgcgCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGc < 4:247381/143‑1 (MQ=255) cAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCg < 5:80464/146‑1 (MQ=255) cAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTAc < 5:30056/149‑1 (MQ=255) ttCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCaa > 2:86624/1‑149 (MQ=255) cGAGCTGGCCGCGGTAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCaa < 4:99438/147‑1 (MQ=255) cTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGAccc < 5:322207/149‑1 (MQ=255) ggCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCgg > 8:64369/1‑149 (MQ=255) ggCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTcgc > 6:135681/1‑149 (MQ=255) aGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCggg > 4:347553/1‑149 (MQ=255) ggCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCgg > 3:77778/1‑149 (MQ=255) cAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGtt > 7:101567/1‑149 (MQ=255) cAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGt > 6:29999/1‑148 (MQ=255) aGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTtc < 2:270740/149‑1 (MQ=255) ctgctgCCGGCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCg < 7:286820/149‑1 (MQ=255) tgctgcCGGCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGt > 2:183793/1‑149 (MQ=255) gcgcCGGCGCTGAACGCCGGCGCCCATATCGA‑CGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGAccc < 6:321581/123‑1 (MQ=255) gcgcCGGCGCTGAACGCCGGCGCCCATATCGA‑CGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGAccc > 5:321581/3‑125 (MQ=255) ctgcCGGCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAg < 7:64369/149‑1 (MQ=255) tgcCGGCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGtt > 6:167536/1‑141 (MQ=255) tgcCGGCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGtt < 5:167536/141‑1 (MQ=255) tgcCGGCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGc > 5:280376/1‑149 (MQ=255) ccGGCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGc < 2:306545/147‑1 (MQ=255) ccGGCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGc < 2:253683/147‑1 (MQ=255) cGGCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTg > 6:6499/1‑149 (MQ=255) ggCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTg > 4:212166/1‑148 (MQ=255) cGTTCAGCGCCGGCGCCCATATCGA‑CGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCAc < 5:29999/149‑1 (MQ=255) ttCAGCGCCGGCGCCCATATCGA‑CGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCACg > 2:338271/1‑148 (MQ=255) | AGCCGCAACTGATCGCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTA‑GTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGA‑CGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCACG > NC_002947/4264738‑4265028 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |