Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,266,148 G→A 7.0% A104V (GCG→GTG)  PP_3738 ← transcriptional regulator VanR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,266,1480GA 7.0% 167.8 / 2.4 57A104V (GCG→GTG) PP_3738transcriptional regulator VanR
Reads supporting (aligned to +/- strand):  ref base G (26/27);  new base A (2/2);  total (28/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01

GCAATGGCCGGGTTGTGGCTGGCGTCGATGATCACCTGATGAAAGCGCATGTTCAGGTCGTGATAGACCTGCAAGTCGTCTTCGCTCACAAAGCCCTTGTCGAACAGCTGATCGCCGTCGACCAGGCATTGTTGCAACGCAGCGCGTGCTTGCACCGACAAGCCACGTTCTGCAGCCTGACGCGCCGCCAACCCCTCCAGCACACCGCGCACCTCGACCGCACCCGCGATGTCATCGGCGCTGACCGAGCGCACCTGAAACCCCCGGCCACCAAAGCGCACCAGCA  >  NC_002947/4266010‑4266295
                                                                                                                                          |                                                                                                                                                   
gCAATGGCCGGGTTGTGGCTGGCGTCGATGATCACCTGATGAAAGCGCATGTTCAGGTCGTGATAGACCTGCAAGTCGTCTTCGCTCACAAAGCCCTTGTCGAACAGCTGATCGCCGTCGACCAGGCATTGTTGCAACGCAGCGCGTGc                                                                                                                                           <  3:72682/149‑1 (MQ=255)
gCAATGGCCGGGTTGTGGCTGGCGTCGATGATCACCTGATGAAAGCGCATGTTCAGGTCGTGATAGACCTGCAAGTCGTCTTCGCTCACAAAGCCCTTGTCGAACAGCTGATCGCCGTCGACCAGGCATTGTTGCAACACAGCGCGTGc                                                                                                                                           >  8:174902/1‑149 (MQ=255)
 cAATGGCCGGGTTGTGGCTGGCGTCGATGATCACCTGATGAAAGCGCATGTTCAGGTCGTGATAGACCTGCAAGTCGTCTTCGCTCACAAAGCCCTTGTCGAACAGCTGATCGCCGTCGA‑CAGGCATTGTTGCAACGCAGCGCGTGCtt                                                                                                                                         <  6:166303/149‑1 (MQ=255)
    tGGCCGGGTTGTGGCTGGCGTCGATGATCACCTGATGAAAGCGCATGTTCAGGTCGTGATAGACCTGCAAGTCGTCTTCGCTCACAAAGCCCTTGTCGAACAGCTGATCGCCGTCGACCAGGCATTGTTGCAACGCAGCGCGTGCTTg                                                                                                                                        >  1:351607/1‑148 (MQ=255)
    tGGCCGGGTTGTGGCTGGCGTCGATGATCACCTGATGAAAGCGCATGTTCAGGTCGTGATAGACCTGCAAGTCGTCTTCGCTCACAAAGCCCTTGTCGAACAGCTGATCGCCGTCGACCAGGCATTGTTGCAACGCAGCGCGTGCTTGc                                                                                                                                       <  2:351607/149‑1 (MQ=255)
                 gctggcGTCGATGATCACCTGATGAAAGCGCATGTTCAGGTCGTGATAGACCTGCAAGTCGTCTTCGCTCACAAAGCCCTTGTCGAACAGCTGATCGCCGTCGACCAGGCATTGTTGCAACGCAGCGCGTGCtt                                                                                                                                         >  6:163635/1‑134 (MQ=255)
                 gctggcGTCGATGATCACCTGATGAAAGCGCATGTTCAGGTCGTGATAGACCTGCAAGTCGTCTTCGCTCACAAAGCCCTTGTCGAACAGCTGATCGCCGTCGACCAGGCATTGTTGCAACGCAGCGCGTGCtt                                                                                                                                         <  5:163635/134‑1 (MQ=255)
                     gcGTCGATGATCACCTGATGAAAGCGCATGTTCAGGTCGTGATAGACCTGCAAGTCGTCTTCGCTCACAAAGCCCTTGTCGAACAGCTGATCGCCGTCGACCAGGCATTGTTTCAACGCAGCGCGTGCTTGCACCGACAAGCCACGTTc                                                                                                                      >  5:94639/1‑149 (MQ=255)
                     gcGTCGATGATCACCTGATGAAAGCGCATGTTCAGGTCGTGATAGACCTGCAAGTCGTCTTCGCTCACAAAGCCCTTGTCGAACAGCTGATCGCCGTCGACCAGGCATTGTTGCAACGCAGCGCGTGCTTGCACCGACAAGCCACGt                                                                                                                        <  4:211755/147‑1 (MQ=255)
                      cGTCGATGATCACCTGATGAAAGCGCATGTTCAGGTCGTGATAGACCTGCAAGTCGTCTTCGCTCACAAAGCCCTTGTCGAACAGCTGATCGCCGTCGACCAGGCATTGTAGCAACGCAGCGCGTGCTTGCACCGACAAGCCACGTTCt                                                                                                                     <  8:98491/149‑1 (MQ=255)
                           atgatCACCTGATGAAAGCGCATGTTCAGGTCGTGATAGACCTGCAAGTCGTCTTCGCTCACAAAGCCCTTGTCGAACAGCTGATCGCCGTCGACCAGGCATTGTTGCAACGCAGCGCGTGCTTGCACCGACAAGCCACGTTCTGCAGc                                                                                                                <  7:67993/149‑1 (MQ=255)
                             gatCACCAGATGAAAGCGCATGTTCAGGTCGTGATAGACCTGCAAGTCGTCTTCGCTCACAAAGCCCTTGTCGAACAGCTGATCGCCGTCGACCAGGCATTGTTGCAACGCAGCGCGTGCTTGCACCGACAATCCACGTTCTGCAGCCt                                                                                                              >  6:337932/1‑149 (MQ=255)
                              atCACCTGATGAAAGCGCATGTTCAGGTCGTGATAGACCTGCAAGTCGTCTTCGCTCACAAAGCCCTTGTCGAACAGCTGATCGCCGTCGACCAGGCATTGTTGCAACACAGCGCGTGCTTGCACCGACAAGCCACGTTCTGCAGCCt                                                                                                              <  7:174902/148‑1 (MQ=255)
                                        gaAAGCGCATGTTCAGGTCGTGATAGACCTGCAAGTCGTCTTCGCTCACAAAGCCCTTGTCGAACAGCTGATCGCCGTCGACCAGGCATTGTTGCAACGCAGCGCGTGCTTGCACCGACAAGCCACGTTCTGCAGCCTGACGcgccgc                                                                                                    >  6:156671/1‑148 (MQ=255)
                                         aaaGCGCATGTTCAGGTCGTGATAGACCTGCAAGTCGTCTTCGCTCACAAAGCCCTTGTCGAACAGCTGATCGCCGTCGACCAGGCATTGTTGCAACGCAGCGCGTGCTTGCACCGACAAGCCACGTTCTGCAGCCTGACGCgccgcc                                                                                                   >  1:178736/1‑148 (MQ=255)
                                                  gTTCAGGTCGTGATAGACCTGCAAGTCGTCTTCGCTCACAAAGCCCTTGTCGAACAGCTGATCGCCGTCGACCAGGCATTGTTGCAACGCAGCGCGTGCTTGCACCGACAAGCCACGTTCTGCAGCCTGACGCGCCGCCAACCCCTc                                                                                           >  8:206847/1‑147 (MQ=255)
                                                      aGGTCGTGATAGACCTGCAAGTCGTCTTCGCTCACAAAGCCCTTGTCGAACAGCTGATCGCCGTCGACCAGGCATTGTTGCAACGCAGCGCGTGCTTGCACCGACAAGCCACGTTCTGCAGCCTGACGCGCCGCCAACCCCTCCAGcac                                                                                     <  7:206847/149‑1 (MQ=255)
                                                             gATAGACCTGCAAGTCGTCTTCGCTCACAAAGCCCTTGTCGAACAGCTGATCGCCGTCGACCAGGCATTGTTGCAACGCAGCGCGTGCTTGCACCGACAAGCCACGTTCTGCAGCCTGACGCGCCGCCAACCCCTCCAGCACACCgcgc                                                                              >  4:181999/1‑149 (MQ=255)
                                                              aTAGACCTGCAAGTCGTCTTCGCTCACAAAGCCCTTGTCGAACAGCTGATCGCCGTCGACCAGGCATTGTTGCAACGCAGCGCGTGCTTGCACCGACAAGCCACGTTCTGCAGCCTGACGCGCCGCCAACCCCTCCAGCACACcgcg                                                                               >  5:350087/1‑147 (MQ=255)
                                                              aTAGACCTGCAAGTCGTCTTCGCTCACAAAGCCCTTGTCGAACAGCTGATCGCCGTCGACCAGGCATTGTTGCAACGCAGCGCGTGCTTGCACCGACAAGCCACGTTCTGCAGCCTGACGCGCCGCCAACCCCTCCAGCACACcgcg                                                                               <  6:350087/147‑1 (MQ=255)
                                                              aTAGACCTGCAAGTCGTCTTCGCTCACAAAGACCTTGTCGAACAGCTGATCGCCGTCGACCAGGCATTGTTGCAACGCAGCGCGTGCTTGCACCGACAAGCCACGTTCTGCAGCCTGACGCGCCGCCAACCCCTCCAGCACACCGCGCa                                                                             >  1:36524/1‑149 (MQ=255)
                                                               tAGACCTGCAAGTCGTCTTCGCTCACAAAGCGCTTGTCGAACAGCTGATCGCCGTCGACCAGGCATTGTTGCAACGCAGCGCGTGCTTGCACCGACAAGCCACGTTCTGCAGCCTGACGCGCCGCCAACCCTTCCAGCACACCGCGCAc                                                                            <  5:337932/149‑1 (MQ=255)
                                                                 gACCTGCAAGTCGTCTTCGCTCACAAAGCCCTTGTCGAACAGCTGATCGCCGTCGACCAGGCATTGTTGCAACGCAGCGCGTGCTTGCACCGACAAGCCACGTTCTGCAGCCTGACGCGCCGCCAACCCCTCCAGCACACCGCGCAcc                                                                           <  4:167624/148‑1 (MQ=255)
                                                                 gACCTGCAAGTCGTCTTCGCTCACAAAGCCCTTGTCGAACAGCTGATCGCCGTCGACCAGGCATTGTTGCAACGCAGCGCGTGCTTGCACCGACAAGCCACGTTCTGCAGCCTGACGCGCCGCCAACCCCTCCAGCACACCGCGCAcc                                                                           <  5:28705/148‑1 (MQ=255)
                                                                   ccTGCAAGTCGTCTTCGCTCACAAAGCCCTTGTCGAACAGCTGATCGCCGTCGACCAGGCATTGTTGCAACGCAGCGCGTGCTTGCACCGACAAGCCACGTTCTGCAGCCTGACGCGCCGCCAACCCCTCCAGCACACCGCGCACCTc                                                                         >  3:73830/1‑148 (MQ=255)
                                                                    cTGCAAGTCGTCTTCGCTCACAAAGCCCTTGTCGAACAGCTGATCGCCGTCGACCAGGCATTGTTGCAACGCAGCGCGTGCTTGCACCGACAAGCCACGTTCTGCAGCCTGACGCGCCGCCAACCCCTCCAGCACACCGCGCACCTCGa                                                                       >  3:14736/1‑149 (MQ=255)
                                                                      gCAAGTCGTCTTCGCTCACAAAGCCCTTGTCGAACAGCTGATCGCCGTCGACCAGGCATTGTTGCAACGCAGCGCGTGCTTGCACCGACAAGCCACGTTCTGCAGCCTGACGCGCCGCCAACCCCTCCAGCACACCGCGCACCTCGAcc                                                                     >  3:318214/1‑149 (MQ=255)
                                                                       gaaGTCGTCTTCGCTCACAAAGCCCTTGTCGAACAGCTGATCGCCGTCGACCAGGCATTGTTGCAACGCAGCGCGTGCTTGCACCGACAAGCCACGTTCTGCAGCCTGACGCGCCGCCAACCCCTCCAGCACACCGCGCACCTCGACCg                                                                    <  8:24548/148‑1 (MQ=255)
                                                                           tcgtcTTCGCTCACAAAGCCCTTGTCGAACAGCTGATCGCCGTCGACCAGGCATTGTTGCAACGCAGCGCGTGCTTGCACCGACAAGCCACGTTCTGCAGCCTGACGCGCCGCCAACCCCTCCAGCACACCGCGCACCTCGACCGCAcc                                                                >  6:112964/1‑149 (MQ=255)
                                                                              tcTTCGCTCACAAAGCCCTTGTCGAACAGCTGATCGCCGTCGACCAGGCATTGTTGCAACGCAGCGCGTGCTTGCACCGACAAGCCACGTTCTGCAGCCTGACGCGCCGCCAACCCCTCCAGCACACCGCGCACCTCGACCGCACCcgc                                                             <  4:73830/149‑1 (MQ=255)
                                                                                  cGCTCACAAAGCCCTTGTCGAACAGCTGATCGCCGTCGACCAGGCATTGTTTCAACGCAGCGCGTGCTTGCACCGACAAGCCACGTTCTGCAGCCTGACGCGCCGCCAACCCCTCCAGCACACCGCGCACCTCGACCGCACCCGCGAt                                                          <  6:94639/148‑1 (MQ=255)
                                                                                  cGCTCACAAAGCCCTTGTCGAACAGCTGATCGCCGTCGACCAGGCATTGTTGCAACGCAGCGCGTGCTTGCACCGACAAGCCACGTTCTGCAGCCTGACGCGCCGCCAACCCCTCCAGCACACCGCGCACCTCGACCGCACCcgcg                                                            <  5:112964/146‑1 (MQ=255)
                                                                                  cGCTCACAAAGCCCTTGTCGAACAGCTGATCGCCGTCGACCAGGCATTGTTGCAACGCAGCGCGTGCTTGCACCGACAAGCCACGTTCTGCAGCCTGACGCGCCGCCAACCCCTCCAGCACACCGCGCACCTCGACCGCACCCGCGAt                                                          <  4:14736/148‑1 (MQ=255)
                                                                                       acaAAGCCCTTGTCGAACAGCTGATCGCCGTCGACCAGGCATTGTTGCAACGCAGCGCGTGCTTGCACCGACAAGCCACGTTCTGCAGCCTGACGCGCCGCCAACCCCTCCAGCACACCGCGCACCTCGACCGCACCCGCGATGTCATc                                                    >  1:351036/1‑149 (MQ=255)
                                                                                        caAAGCCCTTGTCGAACAGCTGATCGCCGTCGACCAGGCATTGTTGCAACGCAGCGCGTGCTTGCACCGACAAGCCACGTTCTGCAGCCTGACGCGCCGCCAACCCCTCCAGCACACCGCGCACCTCGACCGCACCCGCGATGTCATCg                                                   <  5:335744/149‑1 (MQ=255)
                                                                                         aaaGCCCTTGTCGAACAGCTGATCTCCGTCGACCAGGCATTGTTGCAACGCAGCGCGTGCTTGCACCGACAAGCCACGTTCTGCAGCCTGACGCGCCGCCAACCCCTCCAGCACACCGCGCACCTCGACCGCACCCGCGATGTCATCgg                                                  >  2:289901/1‑149 (MQ=255)
                                                                                         aaaGCCCTTGTCGAACAGCTGATCGCCGTCGACCAGGCATTGTTGCAACGCAGCGCGTGCTTGCACCGACAAGCCACGTTCTGCAGCCTGACGCGCCGCCAACCCCTCCAGCACACCGCGCACCTCGACCGCACCCGCGATGTCATCgg                                                  >  1:316920/1‑149 (MQ=255)
                                                                                         aaaGCCCTTGTCGAACAGCTGATCGCCGTCGACCAGGCATTGTTGCAACGCAGCGCGTGCTTGCACCGACAAGCCACGTTCTGCAGCCTGACGCGCCGCCAACCCCTCCAGCACACCGCGCACCTCGACCGCACCCGCGATGTCATCgg                                                  >  5:76647/1‑149 (MQ=255)
                                                                                         aaaGCCCTTGTCGAACAGCTGATCGCCGTCGACCAGGCATTGTTGCAACGCAGCGCGTGCTTGCACCGACAAGCCACGTGCTGCAGCCTGACGCGCCGCCAACCCCTCCAGCACACCGCGCACCTCGACCGCACCCGCGATGTCATCgg                                                  >  2:285585/1‑149 (MQ=255)
                                                                                         aaaGCCCTTGTCGAACAGCTGATCGCCGTCGACCAGGCATTGTTGCAACACAGCGCGTGCTTGCACCGACAAGCCACGTTCTGCAGCCTGACGCGCCGCCAACCCCTCCAGCACACCGCGCACCTCGACCGCACCCGCGATGTCATCgg                                                  >  3:43214/1‑149 (MQ=255)
                                                                                            gCCCTTGTCGAACAGCTGATCGCCGTCGACCAGGCATTGTTGCAACGCAGCGCGTGCTTGCACCGACAAGCCACGTTCTGCAGCCTGACGCGCCGCCAACCCCTCCAGCACACCGCGCACCTCGACCGCACCCGCGATGTCATCGgcgc                                               >  5:172887/1‑149 (MQ=255)
                                                                                              ccTTGTCGAACAGCTGATCGCCGTCGACCAGGCATTGTTGCAACGCAGCGCGTGCTTGCACCGACAAGCCACGTTCTGCAGCCTGACGCGCCGCCAACCCCTCCAGCACACCGCGCACCTCGACCGCACCCGCGATGTCATCGGCGCt                                              >  2:339339/1‑148 (MQ=255)
                                                                                                  gTCGAACAGCTGATCGCCGTCGACCAGGCATTGTTGCAACGCAGCGCGTGCTTGCACCGACAAGCCACGTTCTGCAGCCTGACGCGCCGCCAACCCCTCCAGCACACCGCGCACCTCGACCGCACCCGCGATGTCATCGGCGCTGAcc                                          >  2:157013/1‑148 (MQ=255)
                                                                                                     gAACAGCTGATCGCCGTCGACCAGGCATTGTTGCAACGCAGCGCGTGCTTGCACCGACAAGCCACGTTCTGCAGCCTGAGGCGCCGCCAACCCCTCCAGCACACCGCGCACCTCGACCGCACCCGCGATGTCATCGGCGCTGACCGAgc                                      <  2:63617/149‑1 (MQ=255)
                                                                                                     gAACAGCTGATCGCCGTCGACCAGGCATTGTTGCAACGCAGCGCGTGCTTGCACCGACAAGCCACGTTCTGCAGCCTGACGCGCCGCCAACCCCTCCAGCACACCGCGCACCTCGACCGCACCCGCGATGTCATCGGCGCTGACCGAgc                                      <  4:318214/149‑1 (MQ=255)
                                                                                                     gAACAGCTGATCGCCGTCGACCAGGCATTGTTGCAACGCAGCGCGTGCTTGCACCGACAAGCCACGTTCTGCAGCCTGACGCGCCGCCAACCCCTCCAGCACACCGCGCACCTCGACCGCACCCGCGATGTCATCGGCGCTGACCGAgc                                      <  2:36524/149‑1 (MQ=255)
                                                                                                     gAACAGCTGATCGCCGTCGACCAGGCATTGTTGCAACGCAGCGCGTGCTTGCACCGACAAGCCACGTTCTGCAGCCTGACGCGCCGCCAACCCCTCCAGCACACCGCGCACCTCGACCGCACCCGCGATGTCATCGGCGCTGACCGAg                                       <  5:156671/148‑1 (MQ=255)
                                                                                                           cTGATCGCCGTCGACCAGGCATTGTTGCAACGCAGCGCGTGCTTGCACCGACAAGCCACGTTCTGCAGCCTGACGCGCCGCCAACCCCTCCAGCACACCGCGCACCTCGACCGCACCCGCGATGTCATCGGCGCTGACCGAGCGCACCt                                <  2:178736/149‑1 (MQ=255)
                                                                                                              aTCGCCGTCGACCAGGCATTGTTGCAACGCAGCGCGTGCTTGCACCGACAAGCCACGTTCTGCAGCCTGACGCGCCGCCAACCCCTCCAGCACACCGCGCACCTCGACCGCACCCGCGATGTCATCGGCGCTGACCGAGCGCACCTGa                              <  8:213310/148‑1 (MQ=255)
                                                                                                                   cGTCGACCAGGCATTGTTGCAACGCAGCGCGTGCTTGCACCGACAAGCCACGTTCTGCAGCCTGACGCGCCGCCAACCCCTCCAGCACACCGCGCACCTCGACCGCACCCGCGATGTCATCGGCGCTGACCGAGCGCACCTGAAAcccc                        >  3:240142/1‑149 (MQ=255)
                                                                                                                     tCGACCAGGCATTGTTGCAACGCAGCGCGTGCTTGCACCGACAAGCCACGTTCTGc                                                                                                                   <  2:222612/56‑1 (MQ=255)
                                                                                                                     tCGACCAGGCATTGTTGCAACGCAGCGCGTGCTTGCACCGACAAGCCACGTTCTGc                                                                                                                   >  1:222612/1‑56 (MQ=255)
                                                                                                                        aCCAGGCATTGTTGCAACGCAGCGCGTGCTTGCACCGACAAGCCACGTTCTGCAGCCTGACGCGCCGCCAACCCCTCCAGCACACCGCGCACCTCGACCGCACCCGCGATGTCATCGGCGCTGACCGAGCGCACCTGAAACCCCCGGc                    >  5:318397/1‑148 (MQ=255)
                                                                                                                                  gttgCAACACAGCGCGTGCTTGCACCGACAAGCCACGTTCTGCAGCCTGACGCGCCGCCAACCCCTCCAGCACACCGCGCACCTCGACCGCACCCGCGATGTCATCGGCGCTGACCGAGCGCACCTGAAACCCCCGGCCACCAAAgcg          <  4:43214/148‑1 (MQ=255)
                                                                                                                                       aaCGCAGCGCGTGCTTGCACCGACAAGCCACGTTCTGCAGCCTGACGCGCCGCCAACCCCTCCAGCACACCGCGCACCTCGACCGCACCCGCGATGTCATCGgcgc                                               <  6:130488/106‑1 (MQ=255)
                                                                                                                                       aaCGCAGCGCGTGCTTGCACCGACAAGCCACGTTCTGCAGCCTGACGCGCCGCCAACCCCTCCAGCACACCGCGCACCTCGACCGCACCCGCGATGTCATCGgcgc                                               >  5:130488/1‑106 (MQ=255)
                                                                                                                                         cGCAGCGCGTGCTTGCACCGACAAGCCACGTTCTGCAGCCTGACGCGCCGCCAACCCCTCCAGCACACCGCGCACCTCGACCGCACCCGCGATGTCATCGGCGCTGACCGAGCGCACCTGAAACCCCCGGCCACCAAAGCGCACcagca  <  6:318397/149‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                                   
GCAATGGCCGGGTTGTGGCTGGCGTCGATGATCACCTGATGAAAGCGCATGTTCAGGTCGTGATAGACCTGCAAGTCGTCTTCGCTCACAAAGCCCTTGTCGAACAGCTGATCGCCGTCGACCAGGCATTGTTGCAACGCAGCGCGTGCTTGCACCGACAAGCCACGTTCTGCAGCCTGACGCGCCGCCAACCCCTCCAGCACACCGCGCACCTCGACCGCACCCGCGATGTCATCGGCGCTGACCGAGCGCACCTGAAACCCCCGGCCACCAAAGCGCACCAGCA  >  NC_002947/4266010‑4266295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑