Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,268,299 C→T 8.7% L334L (CTG→CTA)  PP_3740 ← MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,268,2990CT 8.7% 130.2 / 2.2 46L334L (CTG→CTA) PP_3740MFS transporter
Reads supporting (aligned to +/- strand):  ref base C (21/21);  new base T (2/2);  total (23/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.61e-01

CCCAACCCACACCGGTGGCGCGTACCGATGCGTCATAGACCACCGGCGACAAGGCATACAGCCCAGCCACGCAGCCGTTGGAAAACAGCCCGATCAGCAGGCCCAACGCCAGCGCCGCAGTGACCGAGGCACCATTGGCCACGAACAGCACCAGCAGCGCGGCGGTGATGAGCATGAACAACGCCAGCACGCGAGTCAGTGGCCAGCGCGAAGCCAGGCCGCCGATCAGCGCCGCGCCAACGATGCCGCCCACGCTCAACAGCACGCCGCCGGTGATGCCTTGCTGGG  >  NC_002947/4268154‑4268441
                                                                                                                                                 |                                                                                                                                              
cccAACCCACACCGGTGGCGCGTACCGATGCGTCATAGACCACCGGCGACAAGGCATACAGCCCAGCCACGCAGCCGTTGGAAAACAGCCCGATCAGCAGGCCCAACGCCAGCGCCGCAGTGACCGAGGCACCATTGGCCACGAACAGc                                                                                                                                             >  3:128262/1‑149 (MQ=255)
 ccAACCCACACCGGTGGCGCGTACCGATGCGTCATAGACCACCGGCGACAAGACATACAGCCCAGCCACGCAGCCGTTGGAAAACAGCCCGATCAGCAGGCCCAACGCCAGCGCCGCAGTGACCGAGGCACCATTGGCCACGAACAGCa                                                                                                                                            >  1:275635/1‑149 (MQ=255)
    aCCCACACAGGTGGCGCGTACCGATGCGTCATAGACCACCCGCGACAAGGCATACAGCCAAGACACGCAGCCGTTGGAAAACAGCCAGATCAGCAGGCCCAACGCCAGCGCCGCACTGACCGAGGCTCCATTGGACACGAACCccacca                                                                                                                                         >  8:154630/1‑149 (MQ=255)
           ccGGTGGCGCGTACCGATGCGTCATAGACCACCGGCGACAAGGCATACAGCCCAGCCACGCAGCCGTTGGAAAACAGCCCGATCAGCAGGCCCAACGCCAGCGCCGCAGTGACCGAGGCACCATTGGCCACGAACAGCACCAGCAgcgc                                                                                                                                  <  3:153580/149‑1 (MQ=255)
            cGGTGGCGCGTACCGATGCGTCATAGACCACCGGCGACAAGGCATACAGCCCAGCCACGCAGCCGTTGGAAAACAGCCCGATCAGCAGGCCCAACGCCAGCGCCGCAGTGACCGAGGCACCATTGGCCACGAACAGCACCAGCAgcgcc                                                                                                                                 >  7:244214/1‑148 (MQ=255)
             ggTGGCGCGTACCGATGCGTCATAGACCACCGGCGACAAGGCATACAGCCCAGCCACGCAGCCGTTGGAAAACAGCCCGATCAGCAGGCCCAACGCCAGCGCCGCAGTGACCGAGGCACCATTGGCCACGAACAGCACCAGCAGCgcgg                                                                                                                                <  5:5209/149‑1 (MQ=255)
                  cgcgTACCGATGCGTCATAGACCACCGGCGACAAGGCATACAGCCCAGCCACGCAGCCGTTGGAAAACAGCCCGATCAGCAGGCCCAACGCCAGCGCCGCAGTGACCGAGGCACCATTGGCCACGAACAGCACCAGCAGCGCGGCGGtg                                                                                                                           <  4:344508/149‑1 (MQ=255)
                    cgTACCGATGCGTCATAGACCACCGGCGACAAGGCATACAGCCCAGCCACGCAGCCGTTGGAAAACAGCCCGATCAGCAGGCCCAACGCCAGCGCCGCAGTGACCGAGGCACCATTGGCCACGAACAGCACCAGCAGCGCGGCGGtgat                                                                                                                         <  5:324941/149‑1 (MQ=255)
                     gTACCGATGCGTCATAGACCACCGGCGACAAGGCATACAGCCCAGCCACGCAGCCGTTGGAAAACAGCCCGATCAGCAGGCCCAACGCCAGCGCCGCAGTGACCGAGGCACCATTGGCCACGAACAGCACCAGCAGCGCGGCGGtgatg                                                                                                                        <  2:275635/149‑1 (MQ=255)
                      tACCGATGCGTCATAGACCACCGGCGACAAGGCATACAGCCCAGCCACGCAGCCGTTGGAAAACAGCCCGATCAGCAGGCCCAACGCCAGCGCCGCAGTGACCGAGGCACCATTGGCCACGAACAGCACCAGCAGCGCGGCGGtgatga                                                                                                                       <  3:230990/149‑1 (MQ=255)
                           aTGCGTCATAGACCACCGGCGACAAGGCATACAGCCCAGCCACGCAGCCGTTGGAAAACAGCCCGATCAGCAGGCCCAACGCCAGCGCCGCAGTGACCGAGGCACCATTGGCCACGAACAGCACCAGCAGCGCGGCGGTGATGAGCATg                                                                                                                  <  2:86215/149‑1 (MQ=255)
                              cGTCATAGACCACCGGCGACAAGGCATACAGCCCAGCCACGCAGCCGTTGGAAAACAGCCCGATCAGCAGGCCCAACGCCAGCGCCGCAGTGACCGAGGCACCATTGGCCACGAACAGCACCAGCAGCGCGGCGGTGATGAGCATGaac                                                                                                               <  4:128262/149‑1 (MQ=255)
                              cGTCATAGACCACCGGCGACAAGGCATACAGCCCAGCCACGCAGCCGTTGGAAAACAGCCCGATCAGCAGGCCCAACGCCAGCGCCGCAGTGACCGAGGCACCATTGGCCACGAACAGCACCAGCAGCGCGGCGGTGATGAGCATGaa                                                                                                                <  5:300905/148‑1 (MQ=255)
                                tCATAGACCACCGGCGACAAGGCATACAGCCCAGCCACGCAGCCGTTGGAAAACAGCCCGATCAGCAGGCCCAACGCCAGCGCCGCAGTGACCGAGGCACCATTGGCCACGAATAGCACCAGCAGCGCGGCGGTGATGAGCATGaacaa                                                                                                             >  2:242200/1‑149 (MQ=255)
                                tCATAGACCACCGGCGACAAGGCATACAGCCCAGCCACGCAGCCGTTGGAAAACAGCCCGATCAGCAGGCCCAACGCCAGCGCCGCAGTGACCGAGGCACCATTGGCCACGAATAGCACCAGCAGCGCGGCGGTGATGAGCATGaacaa                                                                                                             >  2:247369/1‑149 (MQ=255)
                                tCATAGACCACCGGCGACAAGGCATACAGCCCAGCCACGCAGCCGTTGGAAAACAGCCCGATCAGCAGGCCCAACGCCAGCGCCGCAGTGACCGAGGCACCATTGGCCACGAACAGCACCAGCAGCGCGGCGGTGATGAGCATGaacaa                                                                                                             >  7:158309/1‑149 (MQ=255)
                                        caccGGCGACAAGGCATACAGCCCAGCCACGCAGCCGTTGGAAAACAGCCCGATCAGCAGGCCCAACGCCAGCGCCGCAGTGACCGAGGCACCATTGGCCACGAACAGCACCAGCAGCGCGGCGGTGATGAGCATGAACAACGCCAGCa                                                                                                     >  6:164634/1‑149 (MQ=255)
                                        caccGGCGACAAGGCATACAGCCCAGCCACGCAGCCGTTGGAAAACAGCCCGATCAGCAGGCCCAACGCCAGCGCCGCAGTGACCGAGGCACCATTGGCCACGAACAGCACCAGCAGCGCGGCGGTGATGAGCATGAACAACGCCAGCa                                                                                                     >  6:225956/1‑149 (MQ=255)
                                           cGGCGACAAGGCATACAGCCCAGCCACGCAGCCGTTGGAAAACAGCCCGATCAGCAGGCCCAACGCCAGCGCCGCAGTGACCGAGGCACCATTGGCCACGAATAGCACCAGCAGCGCGGCGGTGATGAGCATGAACAACGCCAGCAcgc                                                                                                  <  1:247369/149‑1 (MQ=255)
                                           cGGCGACAAGGCATACAGCCCAGCCACGCAGCCGTTGGAAAACAGCCCGATCAGCAGGCCCAACGCCAGCGCCGCAGTGACCGAGGCACCATTGGCCACGAATAGCACCAGCAGCGCGGCGGTGATGAGCATGAACAACGCCAGCAcgc                                                                                                  <  1:242200/149‑1 (MQ=255)
                                           cGGCGACAAGGCATACAGCCCAGCCACGCAGCCGTTGGAAAACAGCCCGATCAGCAGGCCCAACGCCAGCGCCGCAGTGACCGAGGCACCATTGGCCACGAACAGCACCAGCAGCGCTGCGGTGATGAGCATGAACAACGCCAGCAcgc                                                                                                  <  8:244214/149‑1 (MQ=255)
                                             gCGACAAGGCATACAGCCCAGCCACGCAGCCGTTGGAAAACAGCCCGATCAGCAGGCCCAACGCCAGCGCCGCAGTGACCGAGGCACCATTGGCCACGAACAGCACCAGCAGCGCGGCGGTGATGAGCATGAACAACGCCAGCAcgc                                                                                                  >  6:155162/1‑147 (MQ=255)
                                             gCGACAAGGCATACAGCCCAGCCACGCAGCCGTTGGAAAACAGCCCGATCAGCAGGCCCAACGCCAGCGCCGCAGTGACCGAGGCACCATTGGCCACGAACAGCACCAGCAGCGCGGCGGTGATGAGCATGAACAACGCCAGCACGCGa                                                                                                <  5:164634/149‑1 (MQ=255)
                                                         aCAGCCCAGCCACGCAGCCGTTGGAAAACAGCCCGATCAGCAGGCCCAACGCCAGCGCCGCAGTGACCGAGGCACCATTGGCCACGAACAGCACCAGCAGCGCGGCGGTGATGAGCATGAACAACGCCAGCACGCGAGTCAGTGGCCAg                                                                                    >  3:177689/1‑149 (MQ=255)
                                                              ccagccACGCAGCCGTTGGAAAACAGCCCGATCAGCAGGCCCAACGCCAGCGCCGCAGTGACCGAGGCACCATTGGCCACGAACAGCACCAGCAGCGCGGCGGTGATGAGCATGAACAACGCCAGCACGCGAGTCAGTGGCCAGcgcg                                                                                >  4:19670/1‑148 (MQ=255)
                                                                agccACGCAGCCGTTGGAAAACAGCCCGATCAGCAGGCCCAACGCCAGCGCCGCAGTGACCGAGGCACCATTGGCCACGAACAGCACCAGCAGCGCGGCGGTGATGAGCATGAACAACGCCAGCACGCGAGTCAGTGGCCAGCGCGAAg                                                                             <  7:154630/149‑1 (MQ=255)
                                                                 gccACGCAGCCGTTGGAAAACAGCCCGATCAGCAGGCCCAACGCCAGCGCCGCAGTGACCGAGGCACCATTGGCCACGAACAGCACCAGCAGCGCGGCGGTGATGAGCATGAACAACGCCAGCACGCGAGTCAGTGGCCAGCGCGAAGc                                                                            >  4:238847/1‑149 (MQ=255)
                                                                    aCGCAGCCGTTGGAAAACAGCCCGATCAGCAGGCCCAACGCCAGCGCCGCAGTGACCGAGGCACCATTGGCCACGAACAGCACCAGCAGCGCGGCGGTGATGAGCATGAACAACGCCAGCACGCGAGTCAGTGGCCAGCGCGAAGCCAg                                                                         <  8:158309/149‑1 (MQ=255)
                                                                          ccGTTGGAAAACAGCCCGATCAGCAGGCCCAACGCCAGCGCCGCAGTGACCGAGGCACCATTGGCCACGAACAGCACCAGCAGCGCGGCGGTGATGAGCATGAACAACGCCAGCACGCGAGTCAGTGGCCAGCGCGAAGCCAGgccgcc                                                                   >  2:221526/1‑149 (MQ=255)
                                                                           cGTTGGAAAACAGCCCGATCAGCAGGCCCAACGCCAGCGCCGCAGTGACCGAGGCACCATTGGCCACGAACAGCACCAGCAGCGCGGCGGTGATGAGCATGAACAACGCCAGCACGCGAGTCAGTGGCCAGCGCGAAGCCAGgccg                                                                     <  7:308727/146‑1 (MQ=255)
                                                                           cGTTGGAAAACAGCCCGATCAGCAGGCCCAACGCCAGCGCCGCAGTGACCGAGGCACCATTGGCCACGAACAGCACCAGCAGCGCGGCGGTGATGAGCATGAACAACGCCAGCACGCGAGTCAGTGGCCAGCGCGAAGCCAGgccg                                                                     >  8:308727/1‑146 (MQ=255)
                                                                               ggAAAACAGCCCGATCAGCAGGCCCAACGCCAGCGCCGCAGTGACCGAGGCACCATTGGCCACGAACAGCACCAGCAGCGCGGCGGTGATGAGCATGAACAACGCCAGCACGCGAGTCAGTGGCCAGCGCGAAGCCAGGCCGCCGATc                                                               <  3:238847/148‑1 (MQ=255)
                                                                                      aGCCCGATCAGCAGGCCCAACGCCAGCGCCGCAGTGACCGAGGCACCATTGGCCACGAACAGCACCAGCAGCGCGGCGGTGATGAGCATGAACAACGCCAGCACGCGAGTCAGTGGCCAGCGCGAAGCCAGGCCGCCGATCAgcgccgc                                                       <  5:225956/149‑1 (MQ=255)
                                                                                                gcagGCCCAACGCCAGCGCCGCAGTGACCGAGGCACCATTGGCCACGAACAGCACCAGCAGCGCGGCGGTGATGAGCATGAACAACGCCAGCACGCGAGTCAGTGGCCAGCGCGaa                                                                              >  8:335771/1‑116 (MQ=255)
                                                                                                gcagGCCCAACGCCAGCGCCGCAGTGACCGAGGCACCATTGGCCACGAACAGCACCAGCAGCGCGGCGGTGATGAGCATGAACAACGCCAGCACGCGAGTCAGTGGCCAGCGCGaa                                                                              <  7:335771/116‑1 (MQ=255)
                                                                                                gcagGCCCAACGCCAGCGCCGCAGTGACCGAGGCACCATTGGCCACGAACAGCACCAGCAGCGCGGCGGTGATGAGCATGAACAACGCCAGCACGCGAGTCAGTGGCCAGCGCGAAGCCAg                                                                         <  2:338248/121‑1 (MQ=255)
                                                                                                gcagGCCCAACGCCAGCGCCGCAGTGACCGAGGCACCATTGGCCACGAACAGCACCAGCAGCGCGGCGGTGATGAGCATGAACAACGCCAGCACGCGAGTCAGTGGCCAGCGCGAAGCCAg                                                                         >  1:338248/1‑121 (MQ=255)
                                                                                                       cAACGCCAGCGCCGCAGTGACCGAGGCACCATTGGCCACGAACAGCACCAGCAGCGCGGCGGTGATGAGCATGAACAACGCCAGCACGCGAGTCAGTGGCCAGCGCGAAGCCAGGCCGCCGATCAGCGCCGCGCCAACGATGCCGCCCa                                      >  5:361105/1‑149 (MQ=255)
                                                                                                               gcgcCGCAGTGACCGAGGCACCATTGGGCACGAACAGCACCAGCAGCGCGGCGTTGATGAGCATGAACAACGCCAGCACGCGAGTCAGTGGCCAGCGCGAAGCCAGGCCGCCGATCAGCGCCGCGCCAACGATGCCGCCCACGCTCAAc                              <  3:293304/149‑1 (MQ=255)
                                                                                                               gcgcCGCAGTGACCGAGGCACCATTGGCCACGAACAGCACCAGCAGCGCGGCGGTGATGAGCATGAACAACGCCAGCACGCGAGTCAGTGGCCAGCGCGAAGCCAGGCCGCCGATCAGCGCCGCGCCAACGATGCCGCCCACGCTCaa                               >  1:144006/1‑148 (MQ=255)
                                                                                                                            cGAGGCACCATTGGCCACGAACAGCACCAGCAGCGCGGCGGTGATGAGCATGAACAACGCCAGCACGCGAGTCAGTGGCCAGCGCGAAGCCAGGCCGCCGATCAGCGCCGCGCCAACGATGCCGCCCACGCTCAACAGCACGCCGCCgg                 >  7:18368/1‑149 (MQ=255)
                                                                                                                                    cATTGGCCACGAACAGCACCAGCAGCGCGGCGGTGATGAGCATGAACAACGCCAGCACGCGAGTCAGTGGCCAGCGCGAAGCCAGGCCGCCGATCAGCGCCGCGCCAACGATGCCGCCCACGCTCAACAGCACGCCGCCGGTGATGCCt         >  1:319682/1‑149 (MQ=255)
                                                                                                                                      ttGGCCACGAACAGCACCAGCAGCGCGGCGGTGATGAGCATGAACAACGCCAGCACGCGAGTCAGTGGCCAGCGCGAAGCCAGGCCGCCGATCAGCGCCGCGCCAACGATGCCGCCCACGCTCAACAGCACGCCGCCGGTGATGCCTTg       <  2:319682/149‑1 (MQ=255)
                                                                                                                                          ccACGAACAGCACCAGCAGCGCGGCGGTGATGAGCATGAACAACGCCAGCACGCGAGTCAGTGGCCAGCGCGAAGCCAGGCCGCCGATCAGCGCCGCGCCAACGATGCCGCCCACGCTCAACAGCACGCCGCCGGTGATGCCTTGCTgg   <  1:221526/149‑1 (MQ=255)
                                                                                                                                           cACGAACAGCACCAGCAGCGCGGCGGTGATGAGCATGAACAACGCCAGCACGCGAGTCAGTGGCCAGCGCGAAGCCAGGCCGCCGATCAGCGCCGCGCCAACGATGCCGCCCACGCTCAACAGCACGCCGCCGGTGATGCCTTGCTggg  >  5:318341/1‑149 (MQ=255)
                                                                                                                                            aCGAACAGCACCAGCAGCGCGGCGGTGATGAGCATGAACAACGCCa                                                                                                        >  2:167149/1‑46 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
CCCAACCCACACCGGTGGCGCGTACCGATGCGTCATAGACCACCGGCGACAAGGCATACAGCCCAGCCACGCAGCCGTTGGAAAACAGCCCGATCAGCAGGCCCAACGCCAGCGCCGCAGTGACCGAGGCACCATTGGCCACGAACAGCACCAGCAGCGCGGCGGTGATGAGCATGAACAACGCCAGCACGCGAGTCAGTGGCCAGCGCGAAGCCAGGCCGCCGATCAGCGCCGCGCCAACGATGCCGCCCACGCTCAACAGCACGCCGCCGGTGATGCCTTGCTGGG  >  NC_002947/4268154‑4268441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑