Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,273,232	C→G	7.2%	L215F (TTG→TTC)	glcC ←	transcriptional regulator GlcC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,273,232	0	C	G	7.2%	206.0 / 2.6	65	L215F (TTG→TTC)	glcC	transcriptional regulator GlcC
Reads supporting (aligned to +/- strand):  ref base C (32/28);  new base G (3/2);  total (35/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGTATGCACCAGCACTGGGTTATGCGACGCCTCACTGATGG  >  NC_002947/4273089‑4273380                                                                                                                                                 |                                                                                                                                                      ccccAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACcg                                                                                                                                                  >  1:218760/1‑149 (MQ=255) ccccAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACcg                                                                                                                                                  >  7:112967/1‑149 (MQ=255)  cccAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACcgc                                                                                                                                                 >  7:204192/1‑149 (MQ=255)         atccatACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CAtgct                                                                                                                                         >  2:93847/1‑149 (MQ=255)             atACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGcc                                                                                                                                      >  5:240855/1‑148 (MQ=255)                      gTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTAGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTCTTCCATGACTGCGCGG‑TACAACCGCG‑CATCCTGCCGATCAATCTg                                                                                                                            >  8:233633/1‑149 (MQ=255)                       tCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTgg                                                                                                                           <  2:218760/149‑1 (MQ=255)                         gCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCg                                                                                                                         >  3:336097/1‑149 (MQ=255)                          cccTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGt                                                                                                                        >  6:231052/1‑149 (MQ=255)                           ccTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGtt                                                                                                                       >  5:321670/1‑149 (MQ=255)                                 cAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTgcg                                                                                                                 >  5:142571/1‑149 (MQ=255)                                 cAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTgcg                                                                                                                 >  8:346276/1‑149 (MQ=255)                                 cAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCA‑CATGCTGCCGATCAATCTGGCGTTTCTgcg                                                                                                                 <  8:288849/149‑1 (MQ=255)                                      gctgctCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTTGc                                                                                                            <  6:321670/149‑1 (MQ=255)                                      gctgctCTTCCTGCTCGATCTCCAGCAAACTGTCGCGTTTGCCGTTGATATGCTCACTGGCCGCCGGCTGCGCCTGGTCGGGCAGTTGTTCGATGACTGCGGGG‑TAGAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTgga                                                                                                            <  7:233633/149‑2 (MQ=255)                                        tgctCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCg                                                                                                          >  7:32276/1‑149 (MQ=255)                                          ctctTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCgg                                                                                                         >  8:4685/1‑148 (MQ=255)                                             ttCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCa                                                                                                       <  3:196216/147‑1 (MQ=255)                                                 tGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTAc                                                                                                   >  6:103607/1‑147 (MQ=255)                                                 tGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACaa                                                                                                 >  1:278882/1‑149 (MQ=255)                                                   cTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACaa                                                                                                 <  8:204192/147‑1 (MQ=255)                                                   cTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACaa                                                                                                 <  7:346276/147‑1 (MQ=255)                                                      gATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAgtt                                                                                              <  1:110076/147‑1 (MQ=255)                                                          tcCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAg                                                                                                <  4:293892/141‑1 (MQ=255)                                                          tcCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAg                                                                                                >  3:293892/1‑141 (MQ=255)                                                           cccGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGAcc                                                                                       >  6:5035/1‑149 (MQ=255)                                                            ccGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCGACATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGttgttg                                                                                          <  1:93847/146‑1 (MQ=255)                                                            ccGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAgttgtt                                                                                           <  8:112967/144‑1 (MQ=255)                                                            ccGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGa                                                                                         <  7:4685/146‑1 (MQ=255)                                                            ccGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGAcc                                                                                       >  3:300226/1‑148 (MQ=255)                                                            ccGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGAcc                                                                                       <  2:335545/148‑1 (MQ=255)                                                             cGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAgttgt                                                                                            >  2:176855/1‑142 (MQ=255)                                                             cGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAgttgt                                                                                            <  1:176855/142‑1 (MQ=255)                                                             cGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGa                                                                                     >  8:44304/1‑149 (MQ=255)                                                                   cTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCaaa                                                                               <  5:103607/149‑1 (MQ=255)                                                                    tGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCaaa                                                                               >  8:90293/1‑148 (MQ=255)                                                                      tCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAacac                                                                            >  3:44764/1‑149 (MQ=255)                                                                         cgTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGt                                                                         >  5:302681/1‑149 (MQ=255)                                                                                    gATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTc                                                               <  7:90293/148‑1 (MQ=255)                                                                                      tatGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAg                                                             <  7:44304/148‑1 (MQ=255)                                                                                       atGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAgg                                                            <  8:330442/148‑1 (MQ=255)                                                                                             aCGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGa                                                     <  6:240855/149‑1 (MQ=255)                                                                                               gcgcCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGc                                                   <  4:336097/149‑1 (MQ=255)                                                                                                   cgcccgcTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGAtt                                               <  7:273383/149‑1 (MQ=255)                                                                                                   cgcccgcTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGAtt                                               <  4:300226/149‑1 (MQ=255)                                                                                                      ccgcTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGCa                                            <  7:115921/149‑1 (MQ=255)                                                                                                        gcTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCGACGTCAGGTCACTGAGCGATTGCAgg                                          <  8:345785/149‑1 (MQ=255)                                                                                                           gcgcCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGTa                                       >  3:264492/1‑149 (MQ=255)                                                                                                             gcCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGTATg                                     >  6:237473/1‑149 (MQ=255)                                                                                                                   tCGGGCAGTTGTTCCATGACTGCGCGGTTACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGTATGCAc                                  >  1:22197/1‑147 (MQ=255)                                                                                                                   tCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACGGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGTATGCACCa                                <  5:5035/148‑1 (MQ=255)                                                                                                                    cGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGTATGCACCAGc                              <  6:302681/149‑1 (MQ=255)                                                                                                                     gggCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGTATGCACCAGCa                             >  3:164631/1‑149 (MQ=255)                                                                                                                         aGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGTATGCACCAGCACTgg                         <  2:278882/149‑1 (MQ=255)                                                                                                                         aGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGTATGCACCAGCACTgg                         <  6:142571/149‑1 (MQ=255)                                                                                                                          gttgttCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGTATGCACCAGCACTggg                        <  5:231052/149‑1 (MQ=255)                                                                                                                           ttgttCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACTCAAACACAGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGTATGCACCAGCACTGGGt                       >  1:90676/1‑149 (MQ=255)                                                                                                                                ccATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGATCGATTGCAGGGTATGCACCAGCACTGGGTTATga                  >  3:30121/1‑148 (MQ=255)                                                                                                                                     catGCGCGG‑TTGTACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGTATGCACCAGCACTGGGTTATGCGACGc             >  6:323562/3‑149 (MQ=255)                                                                                                                                      atGCGCGG‑TTGTACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGTATGCACCAGCACTggg                        <  5:8036/136‑1 (MQ=255)                                                                                                                                      atGCGCGG‑TTGTACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGTATGCACCAGCACTGGGTTATGCGACGcc            >  5:279622/2‑149 (MQ=255)                                                                                                                                      atGCGCGG‑TTGTACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGAGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGTATGCACCAGCACTggg                        >  6:8036/2‑137 (MQ=255)                                                                                                                                       tGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGTATGCACCAGCACTGGGTTATGCGACGCCt           >  5:185771/1‑149 (MQ=255)                                                                                                                                         cgcgG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGTATGCACCAGCACTGGGTTATGCGACGCCTCa         >  6:54096/1‑149 (MQ=255)                                                                                                                                               tACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGAAGGGTATGCACCAGCACTGGGTTATGAGACGCCTCACTGAt    >  3:322902/1‑149 (MQ=255)                                                                                                                                                 cAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGTATGCACCAGCACTGGGTTATGCGACGCCTCACTGATgg  >  4:275452/1‑149 (MQ=255)                                                                                                                                                 cAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGTATGCACCAGCACTGGGTTATGCGACGCCTCACTGATg   <  4:44764/148‑1 (MQ=255)                                                                                                                                                 cAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGTATGCACCAGCACTGGGTTATGCGACGCCTCACTGATg   <  6:185771/148‑1 (MQ=255)                                                                                                                                                 cAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGTATGCACCAGCACTGGGTTATGCGACGCCTCACTGATg   <  5:54096/148‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                      CCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGG‑TACAACCGCG‑CATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGTATGCACCAGCACTGGGTTATGCGACGCCTCACTGATGG  >  NC_002947/4273089‑4273380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑