Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,287,468 C→G 9.5% G90R (GGC→CGC)  PP_3758 ← two component system response regulator/sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,287,4680CG 9.5% 146.8 / 3.2 50G90R (GGC→CGC) PP_3758two component system response regulator/sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base C (23/22);  new base G (3/2);  total (26/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGGCCAAGCACCTTGCGCTGGCGGAACAGGTCGACGAACACCGAGACCTTGCTTTTTACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCGGCATCTGCACATCGAGAATGGCCAGTGCGAACTCGTGCTCCAGCAGCAACGACAAGG  >  NC_002947/4287328‑4287611
                                                                                                                                            |                                                                                                                                               
ccGGCCAAGCACCTTGCGCTGGCGGAACAGGTCGACGAACACCGAGACCTTGCTTTTTACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCcgcg                                                                                                                                          <  4:181694/148‑1 (MQ=255)
ccGGCCAAGCACCTTGCGCTGGCGGAACAGGTCGACGAACACCGAGACCTTGCTTTTTACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCgcgc                                                                                                                                         <  3:79840/149‑1 (MQ=255)
  ggCCAAGCACCTTGCGCTGGCGGAACAGGTCGACGAACACCGAGACCTTGCTTTTTACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCGGGCcgcgaga                                                                                                                                       <  6:231390/149‑4 (MQ=255)
    ccAAGCACCTTGCGCTGGCGGAACAGGTCGACGAACACCGAGACCTTGCTTTTTACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCGGGCcgcgagatg                                                                                                                                     <  1:114741/149‑6 (MQ=255)
    ccAAGCACCTTGCGCTGGCGGAACAGGTCGACGAACACCGAGACCTTGCTTTTTACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGAc                                                                                                                                     <  4:327479/149‑1 (MQ=255)
           ccTTGCGCTGGCGGAACAGGTCGACGAACACCGAGACCTTGCTTTTTACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGAc                                                                                                                               >  5:98281/1‑148 (MQ=255)
                   tGGCGGAACAGGTCGACGAACACCGAGACCTTGCTTTTTACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGAt                                                                                                                      >  5:142767/1‑149 (MQ=255)
                     gCGGAACAGGTCGACGAACACCGAGACCTTGCTTTTTACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGAtg                                                                                                                     <  5:319811/148‑1 (MQ=255)
                      cGGAACAGGTCGACGAACACCGAGACCTTGCTTTTTACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGAtgtg                                                                                                                   <  4:356739/149‑1 (MQ=255)
                      cGGAACAGGTCGACGAACACCGAGACCTTGCTTTTTACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGAtgtg                                                                                                                   >  2:51658/1‑149 (MQ=255)
                      cGGAACAGGTCGACGAACACCGAGACCTTGCTTTTTACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGAtgt                                                                                                                    <  6:142767/148‑1 (MQ=255)
                          aCAGGTCGACGAACACCGAGACCTTGCTTTTTACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTg                                                                                                               <  1:44885/149‑1 (MQ=255)
                           cAGGTCGACGAACACCGAGACCTTGCTTTTTACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACg                                                                                                                                    >  8:323095/1‑127 (MQ=255)
                           cAGGTCGACGAACACCGAGACCTTGCTTTTTACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACg                                                                                                                                    <  7:323095/127‑1 (MQ=255)
                                      acacCGAGACCTTGCTTTTTACGGGCAGGGTGTCGAGCGGCTTGTGCAGAAAGGCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCTCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCg                                                                                                      <  5:230916/146‑1 (MQ=255)
                                      acacCGAGACCTTGCTTTTTACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCGCCGCTCTCGTAGCCCTTGAAGGCATAATTAATCTCTCGGCGCGCCGCGCTGACGAAGACGATCGGGATGTGATAGGTCTTGTCg                                                                                                      >  6:230916/1‑146 (MQ=255)
                                                cTTGCTTTTTACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCa                                                                                         >  2:189660/1‑149 (MQ=255)
                                                 ttGCTTTTTACCTCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCa                                                                                            <  8:239390/145‑1 (MQ=255)
                                                 ttGCTTTTTACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCa                                                                                            >  7:239390/1‑145 (MQ=255)
                                                            cGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGtt                                                                             >  5:208735/1‑149 (MQ=255)
                                                                  ggTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAAc                                                                       >  4:46306/1‑149 (MQ=255)
                                                                    tgtCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCCCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCg                                                                     >  7:308859/1‑149 (MQ=255)
                                                                        gAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTc                                                                  <  3:46306/148‑1 (MQ=255)
                                                                        gAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTc                                                                  <  7:56292/148‑1 (MQ=255)
                                                                        gAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTc                                                                  <  7:242027/148‑1 (MQ=255)
                                                                             gCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGccc                                                            <  1:189660/149‑1 (MQ=255)
                                                                              cTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCg                                                           <  1:51658/149‑1 (MQ=255)
                                                                                   gCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCAAGTTCGAAACCGTTCATGCCCGGCATc                                                      >  5:25669/1‑149 (MQ=255)
                                                                                        aaGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCGGCATCTGcac                                                 <  6:98281/149‑1 (MQ=255)
                                                                                           tCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCGGCATCTGCACATc                                              >  6:77593/1‑149 (MQ=255)
                                                                                             cACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCGGCATc                                                      <  8:187275/139‑1 (MQ=255)
                                                                                             cACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCGGCATc                                                      >  7:187275/1‑139 (MQ=255)
                                                                                             cACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCGGCATCTGCACGTc                                              >  7:78779/1‑147 (MQ=255)
                                                                                             cACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCGGCATCTGCACATc                                              >  1:64102/1‑147 (MQ=255)
                                                                                             cACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCGGCATCTGCACATc                                              >  2:324558/1‑147 (MQ=255)
                                                                                              aCGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCGGCATCTGCACATc                                              >  1:10143/1‑146 (MQ=255)
                                                                                              aCGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCAACTCGCGGCCCGCCGCGCTGACGAAGACGCTCGGGAAGTGCTTGCTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAACCCGTTCATGCCCCGCAGCTGCACATc                                              >  7:272891/1‑146 (MQ=255)
                                                                                               cGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCGGCATCTGCACATCgaga                                          <  3:131996/149‑1 (MQ=255)
                                                                                                 gCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCGGCATCTGCACATCGAGAAt                                        <  7:31663/149‑1 (MQ=255)
                                                                                                     cGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCACCTCGGCCAGTTCGAAACCGTTCATGCCCGGCATCTGCACATCGAGAATGGc                                     >  8:262177/1‑148 (MQ=255)
                                                                                                            gTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCGGCATCTGCACATCGAGAATGGCCAGTGc                               <  6:25669/147‑1 (MQ=255)
                                                                                                             tAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCGGCATCTGCACATCGAGAATGGCCAGTGCGaa                            <  2:64102/149‑1 (MQ=255)
                                                                                                                cccTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCGGCATCTGCACATCGAGAATGGCCAGTGCGAACTc                         <  1:324558/149‑1 (MQ=255)
                                                                                                                 ccTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGCCGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCGGCATCTGCACATCGAGAATGGCCAGTGCGAACTCg                        >  5:288921/1‑149 (MQ=255)
                                                                                                                       aGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCGGCATCTGCACATCGAGAATGGCCAGTGCGAACTCGTGCTc                   >  4:218202/1‑148 (MQ=255)
                                                                                                                                  aTCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCGGCATCTGCACATCGAGAATGGCCAGTGCGAACTCGTGCTCCAGCAGCAACGa       >  1:39042/1‑149 (MQ=255)
                                                                                                                                   cagcgcgGCGGGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCGGCATCTGCACATCGAGAATGGCCAGTGCGAACTCGTGCTCCAGCAGCAACGa       >  6:336559/4‑148 (MQ=255)
                                                                                                                                   cagcgcgGCGGGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGACCGGCATCTGCACATCGAGAATGGCCAGTGCGAACTCGTGCTCCAGCAGCAACGa       >  6:331186/4‑148 (MQ=255)
                                                                                                                                       gcgGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCGGCATCTGCACATCGAGAATGGCCAGTGCGAACTCGTGCTCCAGCAGCAACGACAAgg  >  4:138343/1‑149 (MQ=255)
                                                                                                                                       gcgGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCACGGCATCTGCACATCGAGAATGTCCAGTGCGAACTCGTGATACAGCAGCAACGACAAgg  >  8:156025/1‑149 (MQ=255)
                                                                                                                                            ccgccgCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCGGCATCTGCACATCGAGAATGGCCAGTGCGAACTCGTGCTCCAGCAGCAAc         >  6:179539/1‑137 (MQ=255)
                                                                                                                                            ccgccgCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCGGCATCTGCACATCGAGAATGGCCAGTGCGAACTCGTGCTCCAGCAGCAAc         <  5:179539/137‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                               
CCGGCCAAGCACCTTGCGCTGGCGGAACAGGTCGACGAACACCGAGACCTTGCTTTTTACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCGGCATCTGCACATCGAGAATGGCCAGTGCGAACTCGTGCTCCAGCAGCAACGACAAGG  >  NC_002947/4287328‑4287611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑