Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,299,634 G→A 8.3% A89T (GCA→ACA)  PP_3772 → repressor protein‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,299,6340GA 8.3% 136.4 / 2.7 48A89T (GCA→ACA) PP_3772repressor protein‑like protein
Reads supporting (aligned to +/- strand):  ref base G (22/22);  new base A (2/2);  total (24/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01

CTGGGGGATGGCGCCTGCACACCTTAGCCAGATCCTTTCCGACAAGACCGTGAAAAACCTGGGCGATGACGTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATGCCACGCTGCT  >  NC_002947/4299492‑4299782
                                                                                                                                              |                                                                                                                                                    
cTGGGGGATGGCGCCTGCACACCTTAGCCAGATCCTTTCCGACAAGACCGTGAAAAACCTGGGCGATGACGTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAg                                                                                                                                                <  7:198039/149‑1 (MQ=255)
   ggggATGGCGCCTGCACACCTTAGCCAGATTCTTTCCGACAAGACCGTGAAAAACCTGGGCGATGACGTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGc                                                                                                                                               <  8:326644/147‑1 (MQ=255)
           cgcCTGCACACCTTAGCCAGATCCTTTCCGACAAGACCGTGAAAAACCTGGGCGATGACGTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAg                                                                                                                                     <  6:245715/149‑1 (MQ=255)
            gcCTGCACACCTTAGCCAGATCCTTTCAGACAAGACCGTGAAAAACCTGGGCGATGACGTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGc                                                                                                                                    >  1:9660/1‑149 (MQ=255)
                 cacaCCTTAGCCAGATCCTTTCCGACAAGACCGTGAAAAACCTGGGCGATGACGTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAg                                                                                                                                 <  7:110052/147‑1 (MQ=255)
                   cacCTTAGCCAGATCCTTTCCGACAAGACCGTGAAAAACCTGGGCGATGACGTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGa                                                                                                                             <  2:92947/149‑1 (MQ=255)
                    acCTTAGCCAGATCCTTTCCGACAAGACCGTGAAAAACCTGGGCGATGACGTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGa                                                                                                                             >  2:333520/1‑148 (MQ=255)
                    acCTTAGCCAGATCCTTTCCGACAAGACCGTGAAAAACCTGGGCGATGACGTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGa                                                                                                                             >  4:289170/1‑148 (MQ=255)
                    acCTTAGCCAGATCCTTTCCGACAAGACCGTGAAAAACCTGGGCGATGACGTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGa                                                                                                                             >  7:159358/1‑148 (MQ=255)
                    acCTTAGCCAGATCCTTTCCGACAAGAACGTGAAAAACCTGGGCGATGACGTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACACACAAGCGGCTGTCGAGCCAGGTGa                                                                                                                             >  3:161789/1‑148 (MQ=255)
                      cTTAGCCAGATCCTTTCCGACAAGACCGTGAAAAACCTGGGCGATGACGTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGacaa                                                                                                                          <  6:124052/149‑1 (MQ=255)
                      cTTAGCCAGATCCTTTCCGACAAGACCGTGAAAAACCTGGGCGATGACGTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGacaa                                                                                                                          >  6:306963/1‑149 (MQ=255)
                      cTTAGCCAGATCCTTTCCGACAAGACCGTGAAAAACCTGGGCGATGACGTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGacaa                                                                                                                          <  8:159358/149‑1 (MQ=255)
                       ttAGCCAGATCCTTTCCGACAAGACCGTGAAAAACCTGGGCGATGACGTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGacaa                                                                                                                          >  4:251529/1‑148 (MQ=255)
                         aGCCAGATCCTTTCCGACAAGACCGTGAAAAACCTGGGCGATGACGTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGacaac                                                                                                                         <  3:289170/147‑1 (MQ=255)
                             aGATCCTTTCCGACAAGACCGTGAAAAACCTGGGCGATGACGTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGctc                                                                                                                   >  5:155772/1‑149 (MQ=255)
                               aTCCTTTCCGACAAGACCGTGAAAAACCTGGGCGATGACGTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGa                                                                                                                             <  6:95349/137‑1 (MQ=255)
                               aTCCTTTCCGACAAGACCGTGAAAAACCTGGGCGATGACGTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGa                                                                                                                             >  5:95349/1‑137 (MQ=255)
                               aTCCTTTCCGACAAGACCGTGAAAAACCTGGGCGATGACGTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGctctc                                                                                                                 <  2:238330/149‑1 (MQ=255)
                                  cTTTCCGACAAGACCGTGAAAAACCTGGGCGATGACGTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTg                                                                                                               <  6:155772/148‑1 (MQ=255)
                                          cAAGACCGTGAAAAACCTGGGCGATGACGTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGAcc                                                                                                      >  6:45208/1‑149 (MQ=255)
                                           aaGACCGTGAAAAACCTGGGCGATGACGTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCt                                                                                                     <  3:251529/149‑1 (MQ=255)
                                             gACCGTGAAAAACCTGGGCGATGACGTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTgg                                                                                                   <  8:37110/149‑1 (MQ=255)
                                                  tGAAAAACCTGGGCGATGACGTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACACACAAGCGGCTGTCGAGcc                                                                                                                                   >  7:342681/1‑112 (MQ=255)
                                                  tGAAAAACCTGGGCGATGACGTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACACACAAGCGGCTGTCGAGcc                                                                                                                                   <  8:342681/112‑1 (MQ=255)
                                                           tGGGCGATGACGTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCAGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTg                                                                                     >  4:116492/1‑149 (MQ=255)
                                                                          cccGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCaaa                                                                      <  3:116492/149‑1 (MQ=255)
                                                                               cgcATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCTGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGGCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTAt                                                                 <  5:80523/149‑1 (MQ=255)
                                                                                  aTCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTAtctc                                                              >  6:249359/1‑149 (MQ=255)
                                                                                    cGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCAGAAGCGGCAAAGGTATCTcc                                                             >  5:218198/1‑148 (MQ=255)
                                                                                       gggCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCg                                                            <  6:218198/146‑1 (MQ=255)
                                                                                          cATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGaaa                                                      <  5:306963/149‑1 (MQ=255)
                                                                                           aTCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGAc                                                                                                       >  5:335709/1‑99 (MQ=255)
                                                                                           aTCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGAc                                                                                                       <  6:335709/99‑1 (MQ=255)
                                                                                              gAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCg                                                  >  6:105041/1‑149 (MQ=255)
                                                                                               aaGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCgg                                                 >  2:62132/1‑149 (MQ=255)
                                                                                               aaGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCgg                                                 >  3:284140/1‑149 (MQ=255)
                                                                                                    ttGCCACGGGGCTGGTTCGACGCGCTGTCGGCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCgaa                                                          <  8:236147/135‑1 (MQ=255)
                                                                                                    ttGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCgaa                                                          >  7:236147/1‑135 (MQ=255)
                                                                                                    ttGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAg                                            >  8:245180/1‑149 (MQ=255)
                                                                                                       ccACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGgct                                         >  6:68476/1‑149 (MQ=255)
                                                                                                       ccACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGgc                                          <  5:105041/148‑1 (MQ=255)
                                                                                                         aCGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGgctgc                                       <  1:333520/149‑1 (MQ=255)
                                                                                                              gCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCa                                  >  4:235607/1‑149 (MQ=255)
                                                                                                                  gTTCGACGCGCTGTCGCGAGGCGAACACACACAAGCGGCTGTCGAGCCAGGTGACATCAAGCTCTCTGCAGCCGACCTTGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGTCAAAGGCTGCTGGCAgcg                               <  4:161789/148‑1 (MQ=255)
                                                                                                                   ttCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCAgcggc                             <  7:245180/149‑1 (MQ=255)
                                                                                                                      gACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCAGCGgcgc                           >  2:237518/1‑148 (MQ=255)
                                                                                                                                  ccAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCAGCGGCGCAAGAGCCTg                  >  6:86460/1‑145 (MQ=255)
                                                                                                                                              gCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGGGGGGGGGGGTCCGAAGGAACCCCGCAAAGGATGCTGGCAGCGGCGCAAGAGCCTGCAGATGCCACgctcct  >  1:116816/1‑149 (MQ=255)
                                                                                                                                              gCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCAGCGGCGCaa                         <  2:321544/126‑1 (MQ=255)
                                                                                                                                              gCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCAGCGGCGCaa                         >  1:321544/1‑126 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
CTGGGGGATGGCGCCTGCACACCTTAGCCAGATCCTTTCCGACAAGACCGTGAAAAACCTGGGCGATGACGTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATGCCACGCTGCT  >  NC_002947/4299492‑4299782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑