Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,301,577	G→C	8.1%	intergenic (+67/‑1233)	PP_3774 → / → PP_5627	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,301,577	0	G	C	8.1%	188.3 / 6.2	62	intergenic (+67/‑1233)	PP_3774/PP_5627	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (28/29);  new base C (2/3);  total (30/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.02e-01

CCAGGTCGACTACACCGACGCAACCCCCGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAG‑TACTTCCGCCAGCCTTTACACACTGTACT‑‑GGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCCGCGATGGGTCCGGCGGGCCTGATCT  >  NC_002947/4301429‑4301717                                                                                                                                                     |                                                                                                                                                ccAGGTCGACTACACCGACGCAACCCCCGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGcgcg                                                                                                                                                 >  3:46638/1‑149 (MQ=255)  cAGGTCGACTACACCGACGCAACCCCCGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCgg                                                                                                                                                >  7:233674/1‑149 (MQ=255)    ggTCGACTACACCGACGCAACCCCCGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCgggg                                                                                                                                              >  7:157273/1‑149 (MQ=255)        gACTACACCGACGCAACCCCCGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGgggctgg                                                                                                                                          <  3:184978/149‑1 (MQ=255)        gACTACACCGACGCAACCCCCGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGgggctgg                                                                                                                                          >  7:81183/1‑149 (MQ=255)              acCGACGCAACCCCCGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCt                                                                                                                                    <  4:46638/149‑1 (MQ=255)               ccGACGCAACCCCCGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCCCCGCGCTGGGCttttt                                                                                                                                   <  6:271725/149‑4 (MQ=255)                cGACGCAACCCCCGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCCCCGCGCTGGGCtttttg                                                                                                                                  <  5:187890/149‑5 (MQ=255)                 gACGCAACCCCCGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATc                                                                                                                                 <  5:137110/149‑1 (MQ=255)                  aCGCAACCCCCGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCCCCGCGCTGGGCtttttgca                                                                                                                                <  4:135314/149‑7 (MQ=255)                        cccccGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTggg                                                                                                                          >  2:111593/1‑149 (MQ=255)                        cccccGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTggg                                                                                                                          >  3:182301/1‑149 (MQ=255)                         ccccGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCtttttgca                                                                                                                                <  3:334310/142‑7 (MQ=255)                         ccccGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCtttttgca                                                                                                                                >  4:334310/1‑136 (MQ=255)                         ccccGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGc                                                                                                                         >  4:229509/1‑149 (MQ=255)                          cccGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCt                                                                                                                        >  3:186785/1‑149 (MQ=255)                          cccGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCt                                                                                                                        <  4:186785/149‑1 (MQ=255)                          cccGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCt                                                                                                                        <  8:233674/149‑1 (MQ=255)                           ccgccgAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGgggctggg                                                                                                                                         <  3:122338/131‑1 (MQ=255)                           ccgccgAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGgggctggg                                                                                                                                         >  4:122338/1‑131 (MQ=255)                           ccgccgAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTg                                                                                                                            >  8:235158/1‑144 (MQ=255)                           ccgccgAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTg                                                                                                                            <  7:235158/144‑1 (MQ=255)                                   gACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAG‑TAc                                                                                                              >  2:254385/1‑149 (MQ=255)                                      ggCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAG‑TACTTc                                                                                                           >  2:202516/1‑149 (MQ=255)                                       gCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAG‑TACTTcc                                                                                                          <  2:302926/149‑1 (MQ=255)                                        cTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAG‑TACTTCCg                                                                                                         >  8:4539/1‑149 (MQ=255)                                           gATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAG‑TACTTCCGc                                                                                                        >  5:187174/1‑147 (MQ=255)                                           gATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAG‑TACTTCCGc                                                                                                        <  6:187174/147‑1 (MQ=255)                                              cTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAG‑TACTTCCGCCAGcc                                                                                                   >  7:188333/1‑149 (MQ=255)                                                  atcatcAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAG‑TACTTCCGCCAGCCTTTa                                                                                               <  7:4539/149‑1 (MQ=255)                                                    catcaGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAG‑TACTTCCGCCAGCCTTTaca                                                                                             >  5:334137/1‑149 (MQ=255)                                                       caGCGAGCTCTCGCTCAACCATCGCTGAACGTCGATGCCCAGCACTGCTATCAAAACCACTCAGGGCGTCGCATGAATGCAAAAAGCCAAGCGCGGGGCTCGGCTTCTATCAGCTGGGCTCAACAG‑TACTTCCGCCAGCCTTTacaaaa                                                                                          >  8:135568/1‑146 (MQ=255)                                                           gAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAG‑TACTTCCGCCAGCCTTTACACACTGTa                                                                                      <  3:227979/149‑1 (MQ=255)                                                             gCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAG‑TACTTCCGCCAGCCTTTACACACTGTACt                                                                                    <  8:188333/149‑1 (MQ=255)                                                                   gCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAG‑TACTTCCGCCAGCCTTTACACACTGTACT‑‑GGAAGt                                                                            >  4:19812/1‑149 (MQ=255)                                                                        tccatcGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAG‑TACTTCCGCCAGCCTTTACACACTGTACT‑‑GGAAGTTACTc                                                                       >  8:14515/1‑149 (MQ=255)                                                                        tccatcGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAG‑TACTTCCGCCAGCCTTTACACACTGTACT‑‑GGAAGTTACTc                                                                       <  7:100344/149‑1 (MQ=255)                                                                        tccatcGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAG‑TACTTCCGCCAGCCTTTACACACTGTACT‑‑GGAAGTTACTc                                                                       <  7:14515/149‑1 (MQ=255)                                                                        tccatcGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAG‑TACTTCCGCCAGCCTTTACACACTGTACT‑‑GGAAGTTACTc                                                                       <  8:81183/149‑1 (MQ=255)                                                                         ccatcGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAG‑TACTTCCGCCAGCCTTTACACACTGTACT‑‑GGAAGTTACTc                                                                       >  7:224128/1‑148 (MQ=255)                                                                          catcGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAG‑TACTTCCGCCAGCCTTTACACACTGTACT‑‑GGAAGTTACTCCa                                                                     <  3:229509/149‑1 (MQ=255)                                                                           atcGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAG‑TACTTCCGCCAGCCTTTACACACTGTACT‑‑GGAAGTTACTCCAg                                                                    <  4:182301/149‑1 (MQ=255)                                                                            tcGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAG‑TACTTCCGCCAGCCTTTACACACTGTACT‑‑GGAAGTTACTCCAGt                                                                   <  1:202516/149‑1 (MQ=255)                                                                                tGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAG‑TACTTCCGCCAGCCTTTACACACTGTACT‑‑GGAAGTTACTCCAGTGGgc                                                               >  1:35568/1‑149 (MQ=255)                                                                                tGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAG‑TACTTCCGCCAGCCTTTACACACTGTACT‑‑GGAAGTTACTCCAGTGGgc                                                               >  2:26500/1‑149 (MQ=255)                                                                                            cccAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAG‑TACTTCCGCCAGCCTTTACACACTGTACT‑‑GGAAGTTACTCCAGTGGGCGCTTGTGATaa                                                    <  8:157273/148‑1 (MQ=255)                                                                                             ccAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAG‑TACTTCCGCCAGCCTTTACACACTGTACT‑‑GGAAGTTACTc                                                                       >  1:291798/1‑128 (MQ=255)                                                                                             ccAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAG‑TACTTCCGCCAGCCTTTACACACTGTACT‑‑GGAAGTTACTc                                                                       <  2:291798/128‑1 (MQ=255)                                                                                                gCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAG‑TACTTCCGCCAGCCTTTACACACTGTACT‑‑GGAAGTTACTCCAGTGGGCGCTTGTGATAACtata                                               <  3:19812/149‑1 (MQ=255)                                                                                                 cccTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAG‑TACTTCCGCCAGCCTTTACACACTGTACT‑‑GGAAGTTACTCCAGTGGGCGCTTGTGATAACTATaa                                              <  1:26500/149‑1 (MQ=255)                                                                                                    tGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAG‑TACTTCCGCCAGCCTTTACACACTGTACT‑‑GGAAGTTACTCCAGTGGGCGCTtgt                                                         <  4:51471/135‑1 (MQ=255)                                                                                                    tGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAG‑TACTTCCGCCAGCCTTTACACACTGTACT‑‑GGAAGTTACTCCAGTGGGCGCTtgt                                                         >  3:51471/1‑135 (MQ=255)                                                                                                            aaaCCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAG‑TACTTCCGCCAGCCTTTACACACTGTACT‑‑GGAAGTTACTCCAGTGGGCGCTTCTGATAACTATAACGCTCGGCAtt                                   >  3:300924/1‑149 (MQ=255)                                                                                                             aaCCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAG‑TACTTCCGCCAGCCTTTACACACTGTACT‑‑GGAAGTTACTCCAGTgg                                                                 >  4:356831/1‑118 (MQ=255)                                                                                                             aaCCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAG‑TACTTCCGCCAGCCTTTACACACTGTACT‑‑GGAAGTTACTCCAGTgg                                                                 <  3:356831/118‑1 (MQ=255)                                                                                                                  cTCAGGTCGTCGCATGAATGGAAAAAGCCCAGGGCGGGGCTTGGCTTCTATCAGCTGGGCTGAACAG‑TACTTCCGCCAGCGTTTACACACTGTACT‑‑GGAAGTTACTCCAGTGGGCGGTTGTGATAACTATAACGCTAGGCATTGGATGc                             <  4:300924/149‑1 (MQ=255)                                                                                                                        gCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAG‑TACTTCCGCCAGCCTTTACACACTGTACT‑‑GGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCCGCGa                       >  8:178601/1‑149 (MQ=255)                                                                                                                              cATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAG‑TACTTCCGCCAGCCTTTACACACTGTACT‑‑GGAAGTTACTCCAGTGGGCCCTTGTGATAACTATAACGCTCGGCATTGGATGCCCGCGATGGGTc                 <  1:254385/149‑1 (MQ=255)                                                                                                                                  aaTGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAG‑TACTTCCGCCAGCCTTTACACACTGTACT‑‑GGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCCGCGATGGGTCcggc             <  6:334137/149‑1 (MQ=255)                                                                                                                                     gatagaaGCCCAGCCCCGCGCTGGGCATCTATCAGCTGGGCTGAACAG‑TACTTCCGCCAGCCTTTACACACTGTACT‑‑GGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCCGCGATGGGTCCGGCggg          >  1:10945/6‑149 (MQ=255)                                                                                                                                      cAAAAAGCCCAGCGCGGGGCTGCGCTTCTATCAGCTGCGCTGAACAG‑TACAGACGCCAGCCTTTACACACTGTACT‑‑GGAAGGTACACCACTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCCGCGATGGGTCCGGCGGGc         >  1:89821/1‑149 (MQ=37)                                                                                                                                        agaaGCCCAGCCCCGCGCTGGGCTTTTATCAGCTGGGCTGAACAG‑TACTTCCGCCAGCCTTTACACACTGTACT‑‑GGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCCGCGATGGGTCCGGCGGGCCt       >  1:204199/3‑149 (MQ=255)                                                                                                                                         aaaaCCCCGAGCGGGGGTGGGGTTATATAACCTGGGGTGAAAAGATAATACCCCCCCCTCTTACCCACTTTACTAATGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCCGCGATGGGTCCGGCGGGc         <  1:111593/149‑1 (MQ=255)                                                                                                                                         aaaGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAG‑TACTTCCGCCAGCCTTTACACACTGTACT‑‑GGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCCGCGATGGGTCCGGCGGGCCt       >  3:200508/1‑148 (MQ=255)                                                                                                                                             cccAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAG‑TACTTCCGCCAGCCTTTACACACTGTACT‑‑GGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCCGCGATGGGTCCGGCGGGCCTGAtct  >  3:108970/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                CCAGGTCGACTACACCGACGCAACCCCCGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAG‑TACTTCCGCCAGCCTTTACACACTGTACT‑‑GGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCCGCGATGGGTCCGGCGGGCCTGATCT  >  NC_002947/4301429‑4301717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑