Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,359,592 G→A 6.2% L122L (CTG→TTG)  PP_3834 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,359,5920GA 6.2% 191.2 / 2.0 64L122L (CTG→TTG) PP_3834hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (32/28);  new base A (2/2);  total (34/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01

CTGCCCTCGTCGAGCCGGGCACTGGTCAGCAACAGTACGGTGCAACCGGCGAAGGCAGCATGCCCCTGCAGCTCAGAGACGAACTTCTTGGTGT‑CCAGCGCGGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGCACCACCGCATCCAGCCCGTCCTGCTCTAGGGTATCAAAGGCGCTGACGAACTGGATTTTGTCACCGACCAGCGCCGGGTCGAAGAAGTCCAGCGTGGACAGGTAC  >  NC_002947/4359447‑4359740
                                                                                                                                                  |                                                                                                                                                    
cTGCCCTCGTCGAGCCGGGCACTGGTCAGCAACAGTACGGTGCAACCGGCGAAGGCAGCATGCCCCTGCAGCTCAGAGACGAACTTCTTGGTGT‑CCAGCGCGGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATcagcag                                                                                                                                                   <  8:182922/149‑1 (MQ=255)
    ccTCGTCGAGCCGGGCACTGGTCAGCAACAGTACGGTGAAACCCGCGACGGCAGCATCCCCCTGCAGCTCAGAGACGAACATCATGGTGT‑CCAGCGCGGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTg                                                                                                                                               >  5:63180/1‑149 (MQ=255)
     cTCGTCGAGCCGGGCACTGGTCAGCAACAGTACGGTGCAACCGGCGAAGGCAGCATGCCCCTGCAGCTCAGAGACGAACTTCTTGGTGT‑CCAGCGCGGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAACAGGcca                                                                                                                                               <  3:252067/148‑3 (MQ=255)
     cTCGTCGAGCCGGGCACTGGTCAGCAACAGTACGGTGCAACCGGCGAAGGCAGCATGCCCCTGCAGCTCAGAGACGAACTTCTTGGTGT‑CCAGCGCGGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAACAGGCTgg                                                                                                                                              >  7:291485/1‑149 (MQ=255)
     cTCGTCGAGCCGGGCACTGGTCAGCAACAGTACGGTGCAACCGGCGAAGGCAGCATGCCCCTGCAGCTCAGAGACGAACTTCTTGGTGT‑CCAGCGCGGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAACAGGCTg                                                                                                                                               <  8:291485/148‑1 (MQ=255)
     cTCGTCGAGCCGGGCACTGGTCAGCAACAGTACGGTGCAACCGGCGAAGGCAGCATGCCCCTGCAGCTCAGAGACGAACTTCTTGGTGT‑CCAGCGCGGTTTCCGCACGTACCCGGGCAAAGAGCACACCGTCGACCATCAACATGc                                                                                                                                                 >  4:252067/1‑146 (MQ=255)
        gtcgAGCCGGGCACTGGTCAGCAACAGTATGGTGCAACCGGCGAAGGCAGCATGCCCCTGCAGCTCAGAGACGAACTTCTTGGTGT‑CCAGCGCGGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGcc                                                                                                                                            <  4:151634/148‑1 (MQ=255)
        gtcgAGCCGGGCACTGGTCAGCAACAGTACGGTGCAACCGGCGAAGGCAGCATGCCCCTGCAGCTCAGAGACGAACTTCTTGGTGT‑CCAGCGCGGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGc                                                                                                                                             <  5:106973/147‑1 (MQ=255)
        gtcgAGCCGGGCACTGGTCAGCAACAGTACGGTGCAACCGGCGAAGGCAGCATGCCCCTGCAGCTCAGAGACGAACTTCTTGGTGT‑CCAGCGCGGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCt                                                                                                                                           >  7:181238/1‑149 (MQ=255)
                    aCTGGTCAGCAACAGTACGGTGCAACCGGCGAAGGCAGCATGCCCCTGCAGCTCAGAGACGAACTTCTTGGTGT‑CCAGCGCGGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCaa                                                                                                                               <  6:270630/149‑1 (MQ=255)
                         tCAGCAACAGTACGGTGCAACCGGCGAAGGCAGCATGCCCCTGCAGCTCAGAGACGAACTTCTTGGTGT‑CCAGCGCGGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTcc                                                                                                                          >  6:301306/1‑149 (MQ=255)
                          cAGCAACAGTACGGTGCAACCGGCGAAGGCAGCATGCCCCTGCAGCTCAGAGACGAACTTCTTGGTGT‑CCAGCGCGGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTg                                                                                                                                               <  1:152910/127‑1 (MQ=255)
                          cAGCAACAGTACGGTGCAACCGGCGAAGGCAGCATGCCCCTGCAGCTCAGAGACGAACTTCTTGGTGT‑CCAGCGCGGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTg                                                                                                                                               >  2:152910/1‑127 (MQ=255)
                            gCAACAGTACGGTGCAACCGGCGAAGGCAGCATGCCCCTGCAGCTCAGAGACGAACTTCTTGGTGT‑CCAGCGCGGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTgg                                                                                                                       >  7:244494/1‑149 (MQ=255)
                             cAACAGTACGGTGCAACCGGCGAAGGCAGCATGCCCCTGCAGCTCAGAGACGAACTTCTTGGTGT‑CCAGCGCGGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGgc                                                                                                                      <  5:301306/149‑1 (MQ=255)
                             cAACAGTACGGTGCAACCGGCGAAGGCAGCATGCCCCTGCAGCTCAGAGACGAACTTCTTGGTGT‑CCAGCGCGGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGgc                                                                                                                      >  3:354710/1‑149 (MQ=255)
                                   tACGGTGCAACCGGCGAAGGCAGCATGCCCCTGCAGCTCAGAGACGAACTTCTTGGTGT‑CCAGCGCGGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCgcagc                                                                                                                 >  4:116674/1‑148 (MQ=255)
                                        tGCAACCGGCGAAGGCAGCATGCCCCTGCAGCTCAGAGACGAACTTCTTGGTGT‑CCAGCGCGGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGc                                                                                                           <  3:116674/149‑1 (MQ=255)
                                        tGCAACCGGCGAAGGCAGCATGCCCCTGCAGCTCAGAGACGAACTTCTTGGTGT‑CCAGCGCGGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGc                                                                                                           >  3:56160/1‑149 (MQ=255)
                                           aaCCGGCGAAGGCAGCATGCCCCTGCAGCTCAGAGACGAACTTCTTGGTGT‑CCAGCGCGGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGcacc                                                                                                        <  7:182399/149‑1 (MQ=255)
                                           aaCCGGCGAAGGCAGCATGCCCCTGCAGCTCAGAGACGAACTTCTTGGTGT‑CCAGCGCGGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGcac                                                                                                         >  4:133966/1‑148 (MQ=255)
                                              cGGCGAAGGCAGCATGCCCCTGCAGCTCAGAGACGAACTTCTTGGTGT‑CCAGCGCGGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGCaccacc                                                                                                     >  8:137498/1‑149 (MQ=255)
                                              cGGCGAAGGCAGCATGCCCCTGCAGCTCAGAGACGAACTTCTTGGTGT‑CCAGCGCGGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGCaccacc                                                                                                     <  5:204807/149‑1 (MQ=255)
                                                gCGAAGGCAGCATGCCCCTGCAGCTCAGAGACGAACTTCTTGGTGT‑CCAGCGCGGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGCACCACCGc                                                                                                   <  2:319303/149‑1 (MQ=255)
                                                  gAAGGCAGCATGCCCCTGCAGCTCAGAGACGAACTTCTTGGTGT‑CCAGCGCGGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTTGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGCACCACCTCAt                                                                                                 <  7:120080/149‑1 (MQ=255)
                                                   aaGGCAGCATGCCCCTGCAGCTCAGAGACGAACTTCTTGGTGT‑CCAGCGCGGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTTGCGCAGCAACCGCACCACCGCATc                                                                                                <  7:226335/149‑1 (MQ=255)
                                                          caTGCCCCTGCAGCTCAGAGACGAACTTCTTGGTGTACCAGCGCGGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGCACCACCGCATCCAGccc                                                                                          >  1:240966/1‑149 (MQ=255)
                                                            tGCCCCTGCAGCTCAGAGACGAACTTCTTGGTGT‑CCAGCGCGGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGCACCACCGCATCCAGCCCGTc                                                                                       <  8:244494/149‑1 (MQ=255)
                                                                ccTGCAGCTCAGAGACGAACTTCTTGGTGT‑CCAGCGCGGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGCACCACCGCATCCAGCCCGTCCTGc                                                                                   <  4:56160/149‑1 (MQ=255)
                                                                    cAGCTCAGAGACGAACTTCTTGGTGT‑CCAGCGCGGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGCACCACCGCATCCAGCCCGTCCTGCTCTa                                                                               <  7:137498/149‑1 (MQ=255)
                                                                          agagACGAACTTCTTGGTGT‑CCAGCGCGGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGCACCACCGCATCCAGCCCGTCCTGCTCTAGGGt                                                                           <  8:181238/147‑1 (MQ=255)
                                                                          agagACGAACTTCTTGGTGT‑CCAGCGCGGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGCACCACCGCATCCAGCCCGTCCTGCTCTAGGGTa                                                                          >  7:50090/1‑148 (MQ=255)
                                                                          agagACGAACTTCTTGGTGT‑CCAGCGCGGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGCACCACCGCATCCAGCCCGTCCTGCTCTAGGGTa                                                                          >  7:147645/1‑148 (MQ=255)
                                                                           gagaCGAACTTCTTGGTGT‑CCAGCGCGGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGCACCACCGCATCCAGCCCGTCCTGCTCTAGGGTAt                                                                         >  4:299088/1‑148 (MQ=255)
                                                                             gaCGAACTTCTTGGTGT‑CCAGCGCGGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGCACCAACGCATCCCGCCCGGCCTCCTCTAGGAGATCaa                                                                      >  6:253780/1‑149 (MQ=255)
                                                                             gaCGAACTTCTTCGGGT‑CCAGCGGCGTTTCCGCACGTAGCCGGGCATTGAGCACACCTTCGTCGATCAGCCTGCTGGGCTGTGGGCGGGCAATTTCCTGGCGCAGCAACCGCACCACAGCATCCAGGCCGTCCTGCTCTAGGGTATCaa                                                                      <  6:103913/149‑1 (MQ=255)
                                                                              aCGAACTTCTTGGTGT‑CCAGCGCGGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGAAACCGCACCACCGCATCCAGCCCGTCCTGCTCTAGGGTATCa                                                                       >  1:172393/1‑147 (MQ=255)
                                                                                gAACTTCTTGGTGT‑CCAGCGCGGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGCACCACCGCATCCAGCCCGTCCTGCTCTAGGGTATCAAAgg                                                                   <  3:133966/149‑1 (MQ=255)
                                                                                gAACTTCTTGGTGT‑CCAGCGCGGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGCACCACCGCATCCAGCCCGTCCTGCTCTAGGGTATCAAAgg                                                                   >  2:119485/1‑149 (MQ=255)
                                                                                gAACTTCTTGGTGT‑CCAGCGCGGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGCACCACCGCATCCAGCCCGTCCTGCTCTAGGGTATCAAAgg                                                                   >  6:234354/1‑149 (MQ=255)
                                                                                         ggTGT‑CCAGCGCGGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGCACCACCGCATCCAGCCCGTCCTGctc                                                                                 <  5:309565/126‑1 (MQ=255)
                                                                                         ggTGT‑CCAGCGCGGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGCACCACCGCATCCAGCCCGTCCTGctc                                                                                 >  6:309565/1‑126 (MQ=255)
                                                                                          gtgt‑CCAGCGCGGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGTAGCAACCGCACCACCGCATCCAGCCCGTCCTGCTCTAGGGTATCAAAGGCGCTGACGaa                                                         >  3:186478/1‑149 (MQ=255)
                                                                                            gt‑CCAGCGCGGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGCACCACCGCATCCAGCCCGTCCTGCTCTAGGGTATCAAAGGCGCTGACGAACt                                                       >  2:276095/1‑149 (MQ=255)
                                                                                                cAGCGCGGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGCACCACCGCATCCAGCCCGTCCTGCTCTAgg                                                                             <  3:63329/124‑1 (MQ=255)
                                                                                                cAGCGCGGTTTCCGCAAGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGCACCACCGCATCCAGCCCGTCCTGCTCTAgg                                                                             >  4:63329/1‑124 (MQ=255)
                                                                                                    gcgGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGTAGCAACCGCACCACCGCATCCAGCCCGTCCTGCTCTAGGGTATCAAAGGCGCTGACGAACTGGAtttt                                                <  4:186478/149‑1 (MQ=255)
                                                                                                       gTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGCACCACCGCATCCAGCCCGTCCTGCTCTAGGGTATCAAAGGCGCTGACGAACTGGATTTTGt                                              >  2:338463/1‑148 (MQ=255)
                                                                                                       gTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGCACCACCGCATCCAGCCCGTCCTGCTCTAGGGTATCAAAGGCGCTGACGAACTGGATTTTGTc                                             <  1:338463/149‑1 (MQ=255)
                                                                                                        tttCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGCACCACCGCATCCAGCCCGTCCTGCTCTAGGGTATCAAAGGCGCTGACGAACTGGAtttt                                                <  4:245552/145‑1 (MQ=255)
                                                                                                        tttCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGCACCACCGCATCCAGCCCGTCCTGCTCTAGGGTATCAAAGGCGCTGACGAACTGGAtttt                                                >  3:245552/1‑145 (MQ=255)
                                                                                                           ccGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGCACCACCGCATCCAGCCCGTCCTGCTCTAGGGTATCAAAGGCGCTGACGAACTGGATTTTGTCAcc                                          <  2:240966/148‑1 (MQ=255)
                                                                                                             gCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGCACCACCGCATCCAGCCCGTCCTGCTCTAGGGTATCAAAGGCGCTGACGAACTGGATTTTGTCACCGAc                                       >  3:329383/1‑149 (MQ=255)
                                                                                                                   aCCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGCACCACCGCATCCAGCCCGTCCTGCTCTAGGGTATCAAAGGCGCTGACGAACTGGAtttt                                                <  3:86423/134‑1 (MQ=255)
                                                                                                                   aCCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGCACCACCGCATCCAGCCCGTCCTGCTCTAGGGTATCAAAGGCGCTGACGAACTGGAtttt                                                >  4:86423/1‑134 (MQ=255)
                                                                                                                     ccGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGCACCACCGCATCCAGCCCGTCCTGCTCTAGGGTATCAAAGGCGCTGACGAACTGGATTTTGTCACCGACCAGCGCCg                               <  4:354710/149‑1 (MQ=255)
                                                                                                                             tGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGCACCACCGCATCCAGCCCGTCCTGCTCTAGGGTATCAAAGGCGCTGACGAACTGGATTTTGTCACCGACCAGCGCCggg                             <  5:2529/143‑1 (MQ=255)
                                                                                                                             tGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGCACCACCGCATCCAGCCCGTCCTGCTCTAGGGTATCAAAGGCGCTGACGAACTGGATTTTGTCACCGACCAGCGCCggg                             >  6:2529/1‑143 (MQ=255)
                                                                                                                              gAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGCACCACCGCATCCAGCCCGTCCTGCTCTAGGGTATCAAAGGCGCTGACGAACTGGATTTTGTCACCGACCAGCGCCGGGTCgaag                       >  8:336913/1‑148 (MQ=255)
                                                                                                                               aGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGCACCACCGCATCCAGCCCGTCCTGCTCTAGGGTATCAAAGGCGCTGACGAACTGGATTTTGTCACCGACCAGCGCCGGGTCgaagaa                     <  1:276095/149‑1 (MQ=255)
                                                                                                                               aGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTACCTGGCGCAGCAACCGCACCACCGCATCCAGCCCGTCCTGCTCTAGGGTATCAAAGGCGCTGACGAACTGGATTTTGTCACCGACCAGCGCCGGGTCgaag                       >  8:304241/1‑147 (MQ=255)
                                                                                                                                 cacaCCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGCACCACCGCATCCAGCCCGTCCTGCTCTAGGGTATCAAAGGCGCTGACGAACTGGATTTTGTCACCGACCAGCGCCGGGTCGAAGAAGt                   >  2:159787/1‑149 (MQ=255)
                                                                                                                                    acCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGCACCACCGCATCCAGCCCGTCCTGCTCTAGGGTATCAAAGGCGCTGACGAACTGGATTTTGTCACCGACCAGCGCCGGGTCGAAGAAGTc                  >  6:351024/1‑147 (MQ=255)
                                                                                                                                    acCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGCACCACCGCATCCAGCCCGTCCTGCTCTAGGGTATCAAAGGCGCTGACGAACTGGATTTTGTCACCGACCAGCGCCGGGTCGAAGAAGTCCa                <  4:329383/149‑1 (MQ=255)
                                                                                                                                           acgaTCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGCACCACCGCATCCAGCCCGTCCTGCTCTAGGGTATCAAAGGCGCTGACGATCTAGATTTTGTCACCGACCAGCGACGGGTCGAAGAAGTACAGCGTgg          >  6:199498/1‑148 (MQ=255)
                                                                                                                                                  gcagGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGCACCACCGCATCCAGCCCGTCCTGCTCTAGGGTATCAAAGGCGCTGACGAACTGGATTTTGTCACCGACCAGCGCCGGGTCGAAGAAGTCCAGCGTGGACAGGTAc  >  2:37541/1‑149 (MQ=255)
                                                                                                                                                  gcagGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGCACCACCGCATCCAGCCCGTCCTGCTCTAGGGTATCAAAGGCGCTGACGAACTGGATTTTGTCACCGACCAGCGCCGGGTCGAAGAAGTCCAGCGTGGACAGGTAc  >  3:86913/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CTGCCCTCGTCGAGCCGGGCACTGGTCAGCAACAGTACGGTGCAACCGGCGAAGGCAGCATGCCCCTGCAGCTCAGAGACGAACTTCTTGGTGT‑CCAGCGCGGTTTCCGCACGTACCCGGGCATTGAGCACACCGTCGACGATCAGCAGGCTGGCCTGTTGCCGGGCAATTTCCTGGCGCAGCAACCGCACCACCGCATCCAGCCCGTCCTGCTCTAGGGTATCAAAGGCGCTGACGAACTGGATTTTGTCACCGACCAGCGCCGGGTCGAAGAAGTCCAGCGTGGACAGGTAC  >  NC_002947/4359447‑4359740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑