Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,410,832	T→C	76.9%	L103L (CTA→CTG)	PP_3894 ←	replication protein O

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,410,832	0	T	C	76.9%	115.3 / 32.2	65	L103L (CTA→CTG)	PP_3894	replication protein O
Reads supporting (aligned to +/- strand):  ref base T (7/8);  new base C (25/25);  total (32/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.50e-01

TCGACAACCAATGCGTTAGCATTGGTATTTGTATTTCTGTCTTTTATGTGTGTAATTTTCAACACAGTGGCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGATCGCCCGAGACACGTTGGCGCGCTCGGCAGCGGCCTTCGCTTCATCCGGATACATCATCTTGGCAACGTACAGGG  >  NC_002947/4410689‑4410974                                                                                                                                                |                                                                                                                                               tCGACAACCAATGCGTTAGCATTGGTATTTGTATTTCTGTCTTTTATGTGTGTAATTTTCAACACAGTGGCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGCAGGa                                                                                                                                            >  1:336230/1‑148 (MQ=255)        ccAATGCGTTAGCATTGGTATTTGTATTTCTGTCTTTTATGTGTGTAATTTTCAACACAGTGGCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGCAGGAAAGTGAtt                                                                                                                                    >  7:9282/1‑149 (MQ=255)        ccAATGCGTTAGCATTGGTATTTGTATTTCTGTCTTTTATGTGTGTAATTTTCAACACAGTGGCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGCAGGAAAGTGAtt                                                                                                                                    <  7:172569/149‑1 (MQ=255)           aTGCGTTAGCATTGGTATTTGTATTTCTGTCTTTTATGTGTGTAATTTTCAACACAGTGGCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGCAGGAAAGTGATTggg                                                                                                                                 <  8:9282/149‑1 (MQ=255)                  aGCATTGGTATTTGTATTTCTGTCTTTTATGTGTGTAATTTTCAACACAGTGGCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGa                                                                                                                           <  3:142796/148‑1 (MQ=255)                         gtatttgtatttCTGTCTTTTATGTGTGTAATTTTCAACACAGTGGCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGCAGGAAAGTGATTGGGTCGCGACTgccgc                                                                                                                    >  6:101980/1‑148 (MQ=255)                         gtatttgtatttCTGTCTTTTATGTGTGTAATTTTCAACACAGTGGCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGCAGGAAAGTGATTGGGTCGCGACTgccgc                                                                                                                    >  8:75152/1‑148 (MQ=255)                            tttgtatttCTGTCTTTTATGTGTGTAATTTTCAACACAGTGGCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATc                                                                                                                >  7:146713/1‑149 (MQ=255)                             ttgtatttCTGTCTTTTATGTGTGTAATTTTCAACACAGTGGCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGCAGGAAAGTGATTgg                                                                                                                                  >  8:112203/1‑130 (MQ=255)                             ttgtatttCTGTCTTTTATGTGTGTAATTTTCAACACAGTGGCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGCAGGAAAGTGATTgg                                                                                                                                  <  7:112203/130‑1 (MQ=255)                                    tCTGTCTTTTATGTGTGTAATTTTCAACACAGTGGCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGCAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGaaa                                                                                                         <  5:273870/148‑1 (MQ=255)                                    tCTGTCTTTTATGTGTGTAATTTTCAACACAGTGGCAGGGGTGTTTTCTAAACAGTGTGTATATTTAAACACGGTTGGCTTGGTGTAGTTTATTCAATCAGGTACCGGCAGGAAAGTGATTTGGTCGGGACTGCCGCCATACCGGaa                                                                                                          >  1:271277/1‑147 (MQ=255)                                      tGTCTTTTATGTGTGTAATTTTCAACACAGTGGCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGCAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAg                                                                                                       >  7:69166/1‑148 (MQ=255)                                          ttttATGTGTGTAATTTTCAACACAGTGGCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGCAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCAcgc                                                                                                  >  6:22352/1‑149 (MQ=255)                                          ttttATGTGTGTAATTTTCAAAACAGTGGCAGGGGTGTTTTCTAAACAGTGTGTAGATTTCAACACTGTTGGCTTGCTGTCGATTCTCCACTCAGGTAACGGTAGGAAAGTGATTGTGTCTCGAATTCCGACATAGCGGAAAAGCActc                                                                                                  >  5:114111/1‑147 (MQ=255)                                             tATGTGTGTAATTTTCAACACAGTGGCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGCAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACgcgc                                                                                                <  5:101980/148‑1 (MQ=255)                                                gtgtgtAATTTTCAACACAGTGGCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGa                                                                                             <  8:146713/148‑1 (MQ=255)                                                 tgtgtAATTTTCAACACAGTGGCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGCAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACg                                                                                           >  5:318594/1‑149 (MQ=255)                                                   tgtAATTTTCAACACAGTGGCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGCAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGa                                                                                         >  7:65902/1‑149 (MQ=255)                                                    gtAATTTTCAACACAGTGGCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGCAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGAt                                                                                        >  1:75723/1‑149 (MQ=255)                                                     tAATTTTCAACACAGTGGCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGaaaa                                                                                                        >  1:352341/1‑132 (MQ=255)                                                     tAATTTTCAACACAGTGGCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGaaaa                                                                                                        <  2:352341/132‑1 (MQ=255)                                                     tAATTTTCAACACAGTGGCAGGGGGGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGCAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATc                                                                                       <  8:251278/149‑1 (MQ=255)                                                        ttttCAACACAGTGGCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGCAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAAc                                                                                    <  8:152103/149‑1 (MQ=255)                                                        ttttCAACACAGTGGCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGCAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAAc                                                                                    <  6:172752/149‑1 (MQ=255)                                                          ttCAACACAGTGGCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGCAGGAAAGTGATTGGGTCGCGAATGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACg                                                                                   >  6:20696/1‑148 (MQ=255)                                                            cAACACAGTGGCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAAt                                                                                >  4:86513/1‑149 (MQ=255)                                                               cacaGTGGCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGCAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGa                                                                             >  3:197814/1‑149 (MQ=255)                                                                 caGTGGCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGATc                                                                           <  4:237229/149‑1 (MQ=255)                                                                   gTGGCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCTGGTACCGGCAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGATCGc                                                                         >  7:88836/1‑149 (MQ=255)                                                                      gCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGCAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGATCGccc                                                                       <  5:20696/148‑1 (MQ=255)                                                                       cAGCGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTTGCTTGCTGTCGATTCTCCACTCAGGTACCGGCAGGAAAGTGATTGGGGCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGATCGCCCga                                                                     <  6:87555/149‑1 (MQ=255)                                                                        aGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGCAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGATCGccc                                                                       >  7:335346/1‑146 (MQ=255)                                                                              gtTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGATCGCCCGAGACACGt                                                              >  3:64254/1‑149 (MQ=255)                                                                               ttttCTACACAGTGTGTACATTTCAACACGGTTGGCTTGCAGTCGATTCTCCACTCAGGTACCGGCAGGAAAGACATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTCACGGATCAACGAATTGATCGCCCGAGACAAGtt                                                             >  5:54080/1‑149 (MQ=255)                                                                                 ttCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGCAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGATCGCCCGAGACACGTTg                                                            <  8:335346/148‑1 (MQ=255)                                                                                       acacTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGCAGGaaa                                                                                                                                          >  1:13611/1‑64 (MQ=255)                                                                                       acacTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGCAGGaaa                                                                                                                                          <  2:13611/64‑1 (MQ=255)                                                                                         aGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGCAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGATCGCCCGAGACACGTTGGCGCGCTCg                                                   >  3:279786/1‑149 (MQ=255)                                                                                              gtAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGCAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGaaaa                                                                                                        <  5:205246/91‑1 (MQ=255)                                                                                              gtAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGCAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGaaaa                                                                                                        >  6:205246/1‑91 (MQ=255)                                                                                                     tCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGCAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGATCGCCCGAGACACGTTGGCGCGCTCGGCAGCGGCCtt                                        <  7:75152/148‑1 (MQ=255)                                                                                                       aaCACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGCAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGATCGCCCGAGACACGTTGGCGCGCTCGGCAGCGGCCTTCGc                                     <  4:304438/149‑1 (MQ=255)                                                                                                       aaCACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGCAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGATCGCCCGAGACACGTTGGCGCGCTCGGCAGCGGCCTTCGc                                     >  1:25452/1‑149 (MQ=255)                                                                                                         cacGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGCAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGATCGccc                                                                       <  5:298614/113‑1 (MQ=255)                                                                                                         cacGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGCAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGATCGccc                                                                       >  6:298614/1‑113 (MQ=255)                                                                                                          acGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGaaaa                                                                                                        >  7:83352/1‑79 (MQ=255)                                                                                                          acGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGaaaa                                                                                                        <  8:83352/79‑1 (MQ=255)                                                                                                            ggTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGCAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGATCGCCCGAGACACGTTGGCGCGCTCGGCAGCGGCCTTCGCTTCAt                                <  6:318594/149‑1 (MQ=255)                                                                                                              ttGGCTTGCTGTCGATTCTCCACTCAGGTACCGGCAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGATCGCCCGAGACACGTTGGCGCGCTCGGCAGCGGCCTTCGCTTCATcc                              <  6:362634/149‑1 (MQ=255)                                                                                                                ggCTTGCTGTCGATTCTCCACTCAGGTACCGGCAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGTGGAAAAGCACGCGCTGACGGATCAACGAATTGATCGCCCGAGACACGTTGGCGCGCTCGGCAGCGGCCTTCGCTTCATCCg                             >  6:325359/1‑148 (MQ=255)                                                                                                                 gCTTGCTGTCGATTCTCCACTCAGGTACCGGCAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGATCGCCCGAGACACGTTGGCGCGCTCGGCAGCGGCCTTCGCTTCATCCGGa                           <  2:25452/149‑1 (MQ=255)                                                                                                                      cTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGATCGCCCGAGACACGTTGGCGCGCTCGGCAGCGGCCTTCGCTTCATCCGGATAcat                      >  4:13176/1‑149 (MQ=255)                                                                                                                         tCGATTCTCCACTCAGGTACCGGCAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGATCGCCCGAGACACGTTGGCGCGCTCGGCAGCGGCCTTCGCTTCATCCGGATAcatca                    >  6:298345/1‑148 (MQ=255)                                                                                                                           gATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGATCGCCCGAGACACGTTGGCGCGCTCGGCAGCGGCCTTCGCTTCATCCGGATACATCATCt                 <  4:64254/149‑1 (MQ=255)                                                                                                                           gATTCTCAACTCATGTACCTGTAGGAAAGTGATTGGGTCGCGACTGACGCCATCGAGGAAAAGCACGCGCTGAAGTATCAACGAATTGATCGCCCTAGACACGTTGGCGCGCTCGTCAGCTGACTTAGCTTAATCCGGATACatcatc                  <  6:114111/148‑1 (MQ=255)                                                                                                                             ttCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGATCGCCCGAGACACGTTGGCGCGCTCGGCAGCGGCCTTCGCTTCATCCGGATACATCATCTTg               <  3:86513/149‑1 (MQ=255)                                                                                                                             ttCTCCACTCAGGTACCGGCAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGATCGCCCGAGACACGTTGGCGCGCTCGGCAGCGGCCTTCGCTTCATCCGGATACATCATCTTg               <  4:279786/149‑1 (MQ=255)                                                                                                                              tctcCACTCAGGTACCGGCAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGATCGCCCGAGACACGTTGGCGCGCTCGGCAGCGGCCTTCGCTTCATCCGGATAc                        <  6:95045/139‑1 (MQ=255)                                                                                                                              tctcCACTCAGGTACCGGCAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGATCGCCCGAGACACGTTGGCGCGCTCGGCAGCGGCCTTCGCTTCATCCGGATAc                        >  5:95045/1‑139 (MQ=255)                                                                                                                                  cACTCAGGTACCGGCAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGATCGCCCGAGACACGTTGGCGCGCTCGGCAGCGGCCTTCGCTTCATCCGGATACATCATCTTGGc             <  8:69166/146‑1 (MQ=255)                                                                                                                                    cTCAGGTACCGGCAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGATCGCCCGAGACACGTTGGCGCGCTCGGCAGCGGCCTTCGCTTCATCCGGATACATCATCTTGGCAACg         >  3:44511/1‑148 (MQ=255)                                                                                                                                       aGGTACCGGCAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGATCGCCCGAGACACGTTGGCGCGCTCGGCAGCGGCCTTCGCTTCATCCGGATACATCATCTTGGCAACGTACa     <  5:22352/149‑1 (MQ=255)                                                                                                                                        ggTACCGGCAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGTGGAAAAGCACGCGCTGACGGATCAACGAATTGATCGCCCGAGACACGTTGGCGCGCTCGGCAGCGGCCTTCGCTTCATCCGGATACATCATCTTGGCAACGTACAg    <  5:325359/149‑1 (MQ=255)                                                                                                                                          tACCGGCAGGAAAGTGATTGGGTCGCGACTGCCGCAATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGATCGCCCGAGACACGTTGGCGCGCTCGGCAGCGGCCTTCGCTTCATCCGGATACATCATCTTGGCAACGTACAggg  <  8:65902/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                               TCGACAACCAATGCGTTAGCATTGGTATTTGTATTTCTGTCTTTTATGTGTGTAATTTTCAACACAGTGGCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGATCGCCCGAGACACGTTGGCGCGCTCGGCAGCGGCCTTCGCTTCATCCGGATACATCATCTTGGCAACGTACAGGG  >  NC_002947/4410689‑4410974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑