Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,425,141 G→C 7.7% intergenic (‑199/‑601) PP_5647 ← / → PP_3917 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,425,1410GC 7.7% 206.0 / 5.6 65intergenic (‑199/‑601)PP_5647/PP_3917hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (28/32);  new base C (3/2);  total (31/34)
Fisher's exact test for biased strand distribution p-value = 6.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

TATCCATTTCGCTTAAATGCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTGCGCGC  >  NC_002947/4424993‑4425282
                                                                                                                                                    |                                                                                                                                             
tatCCATTTCGCTTAAATGCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGcgcg                                                                                                                                               >  1:326741/1‑149 (MQ=255)
 atCCATTTCGCTTAAATGCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACGGCGCCAGTAGAGAACCGAGCGCGATCACTGAGCGCGTCCAGCAGACAGAAGGTCAAGTAGCAAAAAGCTCAGCGCgg                                                                                                                                              >  8:263770/1‑149 (MQ=255)
      tttCGCTTAAATGCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGgggctg                                                                                                                                         <  3:87366/149‑1 (MQ=255)
         cGCTTTAATGCGACCGGTGGAATATGATCGGGCCTATTCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGGCCAGCCCCgcgctggg                                                                                                                                       <  4:99854/148‑1 (MQ=255)
          gCTTAAATGCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGAAGACAGAAGATCAAGTAGAAAAAAGCCCAGCGCGgggctggggt                                                                                                                                     >  8:315457/1‑147 (MQ=255)
            ttAAATGCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCGCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTc                                                                                                                                   >  6:363909/1‑149 (MQ=255)
            ttAAATGCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGgggctggg                                                                                                                                       <  8:55925/145‑1 (MQ=255)
            ttAAATGCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGgggctggg                                                                                                                                       >  7:55925/1‑145 (MQ=255)
            ttAAATGCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGACCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTc                                                                                                                                   >  3:36037/1‑149 (MQ=255)
              aaaTGCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCt                                                                                                                                  <  4:227525/148‑1 (MQ=255)
              aaaTGCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCt                                                                                                                                  <  3:147944/148‑1 (MQ=255)
              aaaTGCGAACGGTGGAAAATGATCAGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCt                                                                                                                                  <  3:77376/148‑1 (MQ=255)
                   cGACCGGTTGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTcag                                                                                                                             <  2:326741/148‑1 (MQ=255)
                        ggTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGc                                                                                                                       >  1:207218/1‑149 (MQ=255)
                            gAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGa                                                                                                                   >  5:42336/1‑149 (MQ=255)
                               aaTGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTa                                                                                                                <  1:319118/149‑1 (MQ=255)
                                 tGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGa                                                                                                              <  5:363909/149‑1 (MQ=255)
                                             aTCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCGAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCaa                                                                                                  <  8:265315/149‑1 (MQ=255)
                                             aTCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCaa                                                                                                  <  1:161682/149‑1 (MQ=255)
                                               ccTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAAtt                                                                                                <  2:207218/149‑1 (MQ=255)
                                                cTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAAtt                                                                                                <  4:36037/148‑1 (MQ=255)
                                                     tGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCCCCGCGCTGGGCtttttg                                                                                                                                >  4:23534/1‑107 (MQ=255)
                                                     tGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCCCCGCGCTGGGCtttttg                                                                                                                                <  3:23534/111‑5 (MQ=255)
                                                       atagataTCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTgg                                                                                        >  4:555/1‑149 (MQ=255)
                                                       atagataTCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTgg                                                                                        >  7:83574/1‑149 (MQ=255)
                                                       atagataTCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTgg                                                                                        >  3:268578/1‑149 (MQ=255)
                                                        tagataTCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTgg                                                                                        <  8:83574/148‑1 (MQ=255)
                                                           atatCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGGTTCTGTCAGCAGAGCTCGACt                                                                                                                 <  1:313/120‑1 (MQ=255)
                                                           atatCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACt                                                                                                                 >  2:313/1‑120 (MQ=255)
                                                                aGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTc                                                                               >  2:5793/1‑149 (MQ=255)
                                                                 gCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTg                                                                                                                                 >  4:167600/1‑98 (MQ=255)
                                                                 gCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTg                                                                                                                                 <  3:167600/98‑1 (MQ=255)
                                                                     gACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTgg                                                                                        <  8:215484/135‑1 (MQ=255)
                                                                     gACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTgg                                                                                        >  7:215484/1‑135 (MQ=255)
                                                                      aCAGCGCCAGTAGAGAACCGAGCCCGATCACTGAG‑CCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTgg                                                                                        <  8:47316/133‑1 (MQ=255)
                                                                      aCAGCGCCAGTAGAGAACCGAGCCCGATCACTGAG‑CCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTgg                                                                                        >  7:47316/1‑133 (MQ=255)
                                                                             cAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTtg                                                                  >  5:136224/1‑149 (MQ=255)
                                                                                tAGAGAACCGCGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGa                                                               <  2:346946/149‑1 (MQ=255)
                                                                                      aCCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCta                                                         <  6:136224/149‑1 (MQ=255)
                                                                                        cGAGCCCGATCACTGAGCCCGTCCATCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGTCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCtata                                                       <  7:315457/149‑1 (MQ=255)
                                                                                        cGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCtata                                                       <  6:42336/149‑1 (MQ=255)
                                                                                                 tCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGc                                              <  3:555/149‑1 (MQ=255)
                                                                                                   aCTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGgc                                                                       >  1:245208/1‑122 (MQ=255)
                                                                                                   aCTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGgc                                                                       <  2:245208/122‑1 (MQ=255)
                                                                                                           cGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTc                                                                               >  4:48743/1‑106 (MQ=255)
                                                                                                           cGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTc                                                                               <  3:48743/106‑1 (MQ=255)
                                                                                                            gTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGccc                                   >  2:333236/1‑149 (MQ=255)
                                                                                                                agcagACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGa                               <  4:268578/149‑1 (MQ=255)
                                                                                                                 gcagACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGAt                              <  7:107238/149‑1 (MQ=255)
                                                                                                                 gcagACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGAt                              <  3:285495/149‑1 (MQ=255)
                                                                                                                    gacagaAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATggg                           >  2:6962/1‑149 (MQ=255)
                                                                                                                       agaAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATa                                                             <  3:150935/112‑1 (MQ=255)
                                                                                                                       agaAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATa                                                             >  4:150935/1‑112 (MQ=255)
                                                                                                                       agaAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGt                          >  8:314863/1‑147 (MQ=255)
                                                                                                                       agaAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTcc                        >  1:146994/1‑149 (MQ=255)
                                                                                                                       agaACTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCCATGGGCGCTTGTGATAGCTTTAGCGCTCGGCATTGGATGCCCTCGATGGGTcc                        >  5:267446/1‑149 (MQ=255)
                                                                                                                         aaGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCgg                      >  7:315460/1‑149 (MQ=255)
                                                                                                                               aaGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGa                                                               <  3:9472/102‑1 (MQ=255)
                                                                                                                               aaGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGa                                                               >  4:9472/1‑102 (MQ=255)
                                                                                                                               aaGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATa                                                             <  1:63731/104‑1 (MQ=255)
                                                                                                                               aaGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATa                                                             >  2:63731/1‑104 (MQ=255)
                                                                                                                                    gCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGtt           >  1:185253/1‑149 (MQ=255)
                                                                                                                                        aaaGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTg       <  2:146994/149‑1 (MQ=255)
                                                                                                                                         aaGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTgc      <  8:315460/149‑1 (MQ=255)
                                                                                                                                         aaGCCCAGCCCCGCGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTgc      >  8:313001/1‑149 (MQ=255)
                                                                                                                                         aaGCCCAGCCCCGCGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTgc      >  4:104883/1‑149 (MQ=255)
                                                                                                                                             ccAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCGTTGTTTCTgcgcgc  >  3:333167/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
TATCCATTTCGCTTAAATGCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTGCGCGC  >  NC_002947/4424993‑4425282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑