Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 338,065 G→T 5.1% A168A (GCC→GCA)  PP_0280 ← amino acid ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947338,0650GT 5.1% 293.2 / 3.5 90A168A (GCC→GCA) PP_0280amino acid ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (37/48);  new base T (3/2);  total (40/50)
Fisher's exact test for biased strand distribution p-value = 6.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCAGCTTGAAGCCTTGCACCAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAGCGCCCGTGCCGCTTCAATCTCACCTTTAGGGATCGC  >  NC_002947/337918‑338211
                                                                                                                                                   |                                                                                                                                                  
cagcagCTTGAAGCCTTGCACCAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAggg                                                                                                                                                   >  2:176398/1‑149 (MQ=255)
  gcagcTTGAAGCCTTGCACCAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCa                                                                                                                                                 >  5:86650/1‑149 (MQ=255)
      cTTGAAGCCTTGCACCAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGTGCCCTgg                                                                                                                                             >  5:75155/1‑149 (MQ=255)
          aaGCCTTGCACCAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGTGCCCTGGcca                                                                                                                                          <  6:75155/148‑2 (MQ=255)
           aGCCTTGCACCAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGTGCCCTGGccagt                                                                                                                                        <  7:337551/149‑4 (MQ=255)
            gCCTTGCACCAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCtt                                                                                                                                         >  8:234970/1‑147 (MQ=255)
            gCCTTGCACCAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGa                                                                                                                                       <  7:175171/149‑1 (MQ=255)
             ccTTGCACCAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAg                                                                                                                                      >  5:278425/1‑149 (MQ=255)
                  cACCAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCa                                                                                                                                 <  4:286154/149‑1 (MQ=255)
                   aCCAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAg                                                                                                                                >  6:127591/1‑149 (MQ=255)
                    ccAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAATATTTCCACCGGCAGGTAGGTGCTGGCTATGATTGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCGTCAgg                                                                                                                               <  6:342227/149‑1 (MQ=255)
                     cAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGa                                                                                                                              <  4:346587/149‑1 (MQ=255)
                        cATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCa                                                                                                                           <  6:23127/149‑1 (MQ=255)
                          tGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACt                                                                                                                         >  8:182185/1‑149 (MQ=255)
                              cGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGa                                                                                                                                       >  6:260414/1‑131 (MQ=255)
                              cGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGa                                                                                                                                       <  5:260414/131‑1 (MQ=255)
                               gagaTCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGTCTTGAGCATCAGGATCACTTCGtt                                                                                                                    >  1:307558/1‑149 (MQ=255)
                               gagaTCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGtt                                                                                                                    <  2:59921/149‑1 (MQ=255)
                                agaTCACCAGGGAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTg                                                                                                                   <  8:271693/149‑1 (MQ=255)
                                  aTCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCt                                                                                                                 <  5:127591/149‑1 (MQ=255)
                                     aCCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGa                                                                                                                                       >  5:193316/1‑124 (MQ=255)
                                     aCCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGa                                                                                                                                       <  6:193316/124‑1 (MQ=255)
                                     aCCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTa                                                                                                              >  3:169898/1‑149 (MQ=255)
                                          gTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCg                                                                                                         >  6:117019/1‑149 (MQ=255)
                                          gTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCg                                                                                                         <  4:258982/149‑1 (MQ=255)
                                          gTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCg                                                                                                         >  4:234198/1‑149 (MQ=255)
                                             gAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGc                                                                                                       <  3:353990/148‑1 (MQ=255)
                                                cacGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGACCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGc                                                                                                   <  1:311318/149‑1 (MQ=255)
                                                 acGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGcc                                                                                                  >  4:122630/1‑149 (MQ=255)
                                                        cggcgAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATgcg                                                                                            <  6:224182/148‑1 (MQ=255)
                                                        cggcgAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATgc                                                                                             <  6:278425/147‑1 (MQ=255)
                                                           cgAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCa                                                                                        <  5:117019/149‑1 (MQ=255)
                                                            gaagaaGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGa                                                                                                                                       <  4:21317/101‑1 (MQ=255)
                                                            gaagaaGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGa                                                                                                                                       >  3:21317/1‑101 (MQ=255)
                                                             aagaagATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGt                                                                                                                     <  6:304989/118‑1 (MQ=255)
                                                             aagaagATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGt                                                                                                                     >  5:304989/1‑118 (MQ=255)
                                                             aagaagATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAgc                                                                                      >  2:238475/1‑149 (MQ=255)
                                                                     ttCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGt                                                                                                               <  6:321916/116‑1 (MQ=255)
                                                                     ttCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGt                                                                                                               >  5:321916/1‑116 (MQ=255)
                                                                     ttCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTgg                                                                                >  2:166435/1‑147 (MQ=255)
                                                                        cACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGgcagca                                                                           >  1:302568/1‑149 (MQ=255)
                                                                        cACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGgcagca                                                                           >  5:30472/1‑149 (MQ=255)
                                                                        cACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGgcagc                                                                            <  7:182185/148‑1 (MQ=255)
                                                                        cACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGgcagc                                                                            <  2:358378/148‑1 (MQ=255)
                                                                          ccGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCatg                                                                         <  3:234198/149‑1 (MQ=255)
                                                                              cAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGt                                                                     <  6:30472/149‑1 (MQ=255)
                                                                               aggtaggtGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTa                                                                    <  5:328316/149‑1 (MQ=255)
                                                                               aggtaggtGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCAGCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTa                                                                    <  7:234970/149‑1 (MQ=255)
                                                                                ggtaggtGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAg                                                                   <  3:316458/149‑1 (MQ=255)
                                                                                 agaggtGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATc                                                                                                                                  <  4:211184/83‑1 (MQ=255)
                                                                                  taggtGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGaa                                                                 <  1:192468/149‑1 (MQ=255)
                                                                                    ggtGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAAc                                                                <  7:39812/148‑1 (MQ=255)
                                                                                    ggtGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAAc                                                                <  3:330607/148‑1 (MQ=255)
                                                                                      tGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGc                                                             >  5:97492/1‑149 (MQ=255)
                                                                                         gggCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCa                                                                                        <  2:354595/119‑1 (MQ=255)
                                                                                         gggCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCa                                                                                        >  1:354595/1‑119 (MQ=255)
                                                                                           gCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGtttt                                                        <  1:53891/149‑1 (MQ=255)
                                                                                           gCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGtttt                                                        <  3:122630/149‑1 (MQ=255)
                                                                                           gCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGtttt                                                        <  6:86650/149‑1 (MQ=255)
                                                                                            cGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTg                                                       >  5:16672/1‑149 (MQ=255)
                                                                                            cGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTg                                                       <  1:166435/149‑1 (MQ=255)
                                                                                              atgatgGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGcc                                                     <  2:302568/149‑1 (MQ=255)
                                                                                                 atgGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCAcg                                                  >  5:171365/1‑149 (MQ=255)
                                                                                                  tgGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCAcgc                                                 >  6:108589/1‑149 (MQ=255)
                                                                                                  tgGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCAcgc                                                 >  8:192091/1‑149 (MQ=255)
                                                                                                  tgGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCAcgc                                                 <  4:169898/149‑1 (MQ=255)
                                                                                                    gTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGTCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGa                                               <  2:307558/149‑1 (MQ=255)
                                                                                                          gCCATGCCGGTCAGTTCCAGCAGGGTAACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATAACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGAGGCAGCATGAAGTAGAACAGCGTTTTGCCACGCGACATGcc                                         >  7:297308/1‑149 (MQ=255)
                                                                                                            cATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGcc                                         >  4:202301/1‑147 (MQ=255)
                                                                                                             aTGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAg                                      >  2:269525/1‑149 (MQ=255)
                                                                                                                  ggTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAGCGccc                                 >  3:244501/1‑149 (MQ=255)
                                                                                                                   gTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAGCGCCCg                                >  7:187443/1‑149 (MQ=255)
                                                                                                                    tCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAGCGCCCGt                               <  1:269525/149‑1 (MQ=255)
                                                                                                                    tCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGAGGCAGCATGATGTAGAGCAGCGtgt                                                         >  8:121124/1‑121 (MQ=255)
                                                                                                                      aGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAGCGCCCGTGc                             >  6:124624/1‑149 (MQ=255)
                                                                                                                       gTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAGCGCCCGTGcc                            >  4:100669/1‑149 (MQ=255)
                                                                                                                           cagcagGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAGCGCCCGTGCCGCt                         <  1:176398/148‑1 (MQ=255)
                                                                                                                           cagcagGGTCACGGGACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAGCGCCCGTGCCGCt                         <  5:51618/148‑1 (MQ=255)
                                                                                                                               agGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAGCGCCCGTGCCGCTTCAAt                    <  3:100669/149‑1 (MQ=255)
                                                                                                                                 ggTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAGCGCCCGTGCCGCTTCAAtct                  >  7:256675/1‑149 (MQ=255)
                                                                                                                                        gTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAGCGCCCGTGCCGCTTCAATCTCACCttt           <  6:97492/149‑1 (MQ=255)
                                                                                                                                        ctcaaggCCAGTGCCCTGGCCTTGCGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCCCGCGACATACCCAGCGCCAGTGCCGCTTCCATCTCCCCtg            >  1:364461/6‑147 (MQ=255)
                                                                                                                                         tACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAGCGCCCGTGCCGCTTCAATCTCACCTTTa          <  3:202301/149‑1 (MQ=255)
                                                                                                                                           cTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAGCGCCCGTGCCGCTTCAATCTCACCTTTAgg        <  5:108589/149‑1 (MQ=255)
                                                                                                                                           cTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAGCGCCCGTGCCGCTTCAATCTCACCTTTAgg        <  6:171365/149‑1 (MQ=255)
                                                                                                                                           cTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAGCGCCCGTGCCGCTTCAATCTCACCTTTAg         <  8:187443/148‑1 (MQ=255)
                                                                                                                                             ggCCAGTGCCCTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAGCGCCCGTGCCGCTTCAATCTCACCTTTAGGGa      >  3:339554/1‑149 (MQ=255)
                                                                                                                                              gCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAGCGCCCGTGCCGCTTCAATCTCACCTTTAGGGAt     >  3:223737/1‑149 (MQ=255)
                                                                                                                                              gCCAGGGCACTGGCCTTGAGCATCAGGATCAATTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATCCCCACCGCCCGTGCCGCTTAAATCTCACATTTAGTGAt     >  8:266405/1‑149 (MQ=255)
                                                                                                                                                cAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAGCGCCCGTGCCGCTTCAATCTCACCTTTAGGGATCg   >  5:253639/1‑149 (MQ=255)
                                                                                                                                                 aGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAGCGCCCGTGCCGCTTCAATCTCACCTTTAGGGATCGc  <  5:124624/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CAGCAGCTTGAAGCCTTGCACCAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAAGAAGATTTCCACCGGCAGGTAGGTGCGGGCGATGATGGTACGGGCCATGCCGGTCAGTTCCAGCAGGGTCACGGTACTGGCCAGGGCACTGGCCTTGAGCATCAGGATCACTTCGTTGCTGTACGCCGGCAGGCCGATGCGCGCAGCGCGTGGCAGCATGATGTAGAACAGCGTTTTGCCACGCGACATGCCCAGCGCCCGTGCCGCTTCAATCTCACCTTTAGGGATCGC  >  NC_002947/337918‑338211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑