Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,467,805 G→A 7.9% W17* (TGG→TAG) ‡ PP_3959 → voltage‑gated chloride channel family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,467,8050GA 7.9% 181.5 / 4.1 63W17* (TGG→TAG) ‡PP_3959voltage‑gated chloride channel family protein
Reads supporting (aligned to +/- strand):  ref base G (28/30);  new base A (2/3);  total (30/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01

GCCACTCCCGGTGCGGCTACAACCTGTGTCGGGTAGGCCTGTGCGGGAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCT‑ACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAGCGCTTGCTGGAATGGGCACCCTGGTCACCTCTTCT  >  NC_002947/4467663‑4467946
                                                                                                                                               |                                                                                                                                             
gCCACTCCCGGTGCGGCTACAACCTGTGTCGGGTAGGCCTGTGCGGGAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCT‑ACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCgag                                                                                                                                          >  5:338560/1‑148 (MQ=255)
 ccACTCCCGGTGCGGCTACAACCTGTGTCGGGTAGGCCTGTGCGGGAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCT‑ACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGACgccat                                                                                                                                        >  2:15239/1‑145 (MQ=255)
  cACTCCCGGTGCGGCTACAACCTGTGTCGGGTAGGCCTGTGCGGGAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGTCCCT‑ACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAAt                                                                                                                                       <  4:152494/149‑1 (MQ=255)
   aCTCCCGGTGCGGCTACAACCTGTGTCGGGTAGGCCTGTGCGGGAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCTCACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAAt                                                                                                                                       <  1:261666/149‑1 (MQ=255)
   aCTCCCGGTGCGGCTACAACCTGTGTCGGGTAGGCCTGTGCGGGAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCT‑ACCCGACCTGCACCTTCACTCCCCCGCCATTCTCGCCAGCGTGaacg                                                                                                                                      >  2:208966/1‑147 (MQ=255)
     tCCCGGTGCGGCTACAACCTGTGTCGGGTAGGCCTGTGCGGGAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCT‑ACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGc                                                                                                                                    >  8:56121/1‑149 (MQ=255)
       ccGGTGCGGCTACAACCTGTGTCGGGTAGGCCTGTGCGGGAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCT‑ACCCGACCTGCACCTTCACTCCCCCGCCATTCTCGCCAGCGTGAACGGc                                                                                                                                    <  1:208966/147‑1 (MQ=255)
       ccGGTGCGGCTACAACCTGTGTCGGGTAGGCCTGTGCGGGAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCT‑ACCCGACCTGCACCTTCACTCCCCCGCCATTCTCGCCAGCGTGAACGGCgg                                                                                                                                  <  6:166871/149‑2 (MQ=255)
              ggCTACAACCTGTGTCGGGTAGGCCTGTGCGGGAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCT‑ACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGAc                                                                                                                           <  6:207032/149‑1 (MQ=255)
               gCTACAACCTGTGTCGGGTAGGCCTGTGCTGGAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCT‑ACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACt                                                                                                                          <  7:56121/149‑1 (MQ=255)
               gCTACAACCTGTGTCGGGTAGGCCTGTGCGGGAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCT‑ACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACt                                                                                                                          <  7:33902/149‑1 (MQ=255)
                       cTGTGTCGGGTAGGCCTGTGCGGGAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCT‑ACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCt                                                                                                                  <  7:182576/149‑1 (MQ=255)
                          tgtCGGGTAGGCCTGTGCGGGAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCT‑ACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTg                                                                                                                 <  6:76826/147‑1 (MQ=255)
                              gggTAGGCCTGTGCGGGAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCT‑ACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCgg                                                                                                           <  3:82128/149‑1 (MQ=255)
                               ggTAGGCCTGTGCGGGAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCT‑ACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCggg                                                                                                          <  2:101969/149‑1 (MQ=255)
                                     ccTGTGCGGGAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCT‑ACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCt                                                                                                                  <  4:204979/135‑1 (MQ=255)
                                     ccTGTGCGGGAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCT‑ACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCt                                                                                                                  >  3:204979/1‑135 (MQ=255)
                                         tgCGGGAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCT‑ACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCggg                                                                                                          >  3:323027/1‑139 (MQ=255)
                                         tgCGGGAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCT‑ACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCggg                                                                                                          <  4:323027/139‑1 (MQ=255)
                                         tgCGGGAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCT‑ACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCtgg                                                                                                >  6:274549/1‑149 (MQ=255)
                                            gggAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCT‑ACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGTGTTCTGGATCGGGGCATTGCtggtg                                                                                              <  3:358987/148‑1 (MQ=255)
                                            gggAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCT‑ACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGTGTTCTGGATCGGGGCATTGCtggtg                                                                                              >  4:358987/1‑148 (MQ=255)
                                              gAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGAGAATGTCCGACCCT‑ACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTgggg                                                                                           >  1:254141/1‑149 (MQ=255)
                                              gAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCT‑ACCCGACCTGCACCTTCACTCCCCCGCCCTACACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTCGATCGGGGCATTGCtggtggcc                                                                                           >  2:305615/1‑147 (MQ=255)
                                               aaaaCACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCT‑ACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGc                                                                                          <  7:7825/149‑1 (MQ=255)
                                                      gCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCT‑ACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTgc                                                                                   >  7:163192/1‑149 (MQ=255)
                                                             tCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCT‑ACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCt                                                                            >  3:24727/1‑149 (MQ=255)
                                                             tCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCT‑ACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCt                                                                            >  3:341128/1‑149 (MQ=255)
                                                              cACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCT‑ACCCGACCTGCACCTTCACTCCCCCGCCATTCTCGCCAGCGTGAACGGCgg                                                                                                                                  <  6:299177/94‑2 (MQ=37)
                                                              cACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCT‑ACCCGACCTGCACCTTCACTCCCCCGCCATTCTCGCCAGCGTGAACGGCgg                                                                                                                                  >  5:299177/1‑93 (MQ=37)
                                                               aCGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCT‑ACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCttt                                                                          >  8:67724/1‑149 (MQ=255)
                                                               aCGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCT‑ACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCttt                                                                          <  8:163192/149‑1 (MQ=255)
                                                                 ggCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCT‑ACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCg                                                                        >  2:181815/1‑149 (MQ=255)
                                                                                 cATGTGTGGACAATGTCCGACCCT‑ACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGAcgc                                                        <  2:174754/149‑1 (MQ=255)
                                                                                         gACAATGTCCGACCCT‑ACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTc                                                 <  5:147855/148‑1 (MQ=255)
                                                                                         gACAATGTCCGACCCT‑ACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTc                                                 >  6:147855/1‑148 (MQ=255)
                                                                                         gACAATGTCCGACCCT‑ACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCg                                                <  6:338560/149‑1 (MQ=255)
                                                                                          aCAATGTCCGACCCT‑ACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGc                                               >  1:11456/1‑149 (MQ=255)
                                                                                            aaTGTCCGACCCT‑ACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGc                                                                             <  4:24929/117‑1 (MQ=255)
                                                                                            aaTGTCCGACCCT‑ACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGc                                                                             >  3:24929/1‑117 (MQ=255)
                                                                                            aaTGTCCGACCCT‑ACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCtt                                             >  7:159397/1‑149 (MQ=255)
                                                                                              tGTCCGACCCT‑ACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCg                                           >  2:95471/1‑149 (MQ=255)
                                                                                                 ccGACCCT‑ACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGGGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGtt                                         >  7:91551/1‑148 (MQ=255)
                                                                                                   gACCCT‑ACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCaa                                      <  4:24727/149‑1 (MQ=255)
                                                                                                     cccT‑ACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAgc                                    <  2:11456/149‑1 (MQ=255)
                                                                                                     cccT‑ACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAgc                                    >  8:265554/1‑149 (MQ=255)
                                                                                                       cT‑ACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGc                                                                                                                                    >  5:17629/1‑51 (MQ=255)
                                                                                                       cT‑ACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGc                                                                                                                                    <  6:17629/51‑1 (MQ=255)
                                                                                                       cT‑ACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAgcgc                                  <  1:181815/149‑1 (MQ=255)
                                                                                                           cccGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAGCGCTTg                               <  4:341128/149‑1 (MQ=255)
                                                                                                              gACCTGCACCATCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTACTGGATCGGGGAATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAGCGCTTGCTg                            >  4:76357/1‑149 (MQ=255)
                                                                                                                   gCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAGCGCTTGCTGGAATg                       >  7:32644/1‑149 (MQ=255)
                                                                                                                     aCCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAGCGCTTGCTGGAATggg                     <  8:91551/149‑1 (MQ=255)
                                                                                                                       cTTCACTCCGCCGCCGTTCACGCTGGCGAGTATGGCGTCAGCGTCTGGCGTTCTGGATCGGGGCTTTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGc                                                                  <  6:247363/102‑1 (MQ=255)
                                                                                                                        ttCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAGCGCTTGCTGGAATGGGCAc                  >  5:49938/1‑149 (MQ=255)
                                                                                                                           aCTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAGCGCTTGCTGGAATGGGCACCCt               >  3:164800/1‑149 (MQ=255)
                                                                                                                              cccccGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAGCGCTTGCTg                            <  2:295963/133‑1 (MQ=255)
                                                                                                                              cccccGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAGCGCTTGCTg                            >  1:295963/1‑133 (MQ=255)
                                                                                                                                  cgccgTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAGCGCTTGCTGGAAt                        >  7:325289/1‑133 (MQ=255)
                                                                                                                                  cgccgTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAGCGCTTGCTGGAAt                        <  8:325289/133‑1 (MQ=255)
                                                                                                                                    ccgTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAGCGCTTGCTGGAATGGGCACCCTGGTCACctc      <  5:274549/149‑1 (MQ=255)
                                                                                                                                      gTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAGCGCTTGCTGGAATGGGCACCCTGGTCACCtctt    <  8:32644/149‑1 (MQ=255)
                                                                                                                                         cACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAGCGCTTGCTGGAATGGGCACCCTGGTCACCtcttct  <  4:153235/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                             
GCCACTCCCGGTGCGGCTACAACCTGTGTCGGGTAGGCCTGTGCGGGAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCT‑ACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAGCGCTTGCTGGAATGGGCACCCTGGTCACCTCTTCT  >  NC_002947/4467663‑4467946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑