Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,521,834 C→T 7.5% intergenic (‑97/‑318) icd ← / → idh NADP(+)‑specific isocitrate dehydrogenase/isocitrate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,521,8340CT 7.5% 153.9 / 2.3 53intergenic (‑97/‑318)icd/idhNADP(+)‑specific isocitrate dehydrogenase/isocitrate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (27/22);  new base T (2/2);  total (29/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

GACGGTAATTTTGGTGCCGTCGGTCGGAACCTTGATTTTCTGGTATCCCATGCTTGCACTACTCCGCTGTCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGCCCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATGAAT  >  NC_002947/4521687‑4521980
                                                                                                                                                   |                                                                                                                                                  
gACGGTAATTTTGGTGCCGTCGGTCGGAACCTTGATTTTCTGGTATCCCATGCTTGCACTACTCCGCTGTCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACccgcc                                                                                                                                                    <  6:121959/148‑1 (MQ=255)
             gTGCCGTCGGTCGGAACCTTGATTTTCTGGTATCCCATGCTTGCACTACTCCGCTGTCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTa                                                                                                                                       <  2:16421/148‑1 (MQ=255)
                ccGTCGGTCGGAACCTTGATTTTCTGGTATCCCATGCTTGCACTACTCCGCTGTCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCAt                                                                                                                                                >  1:54752/1‑136 (MQ=255)
                ccGTCGGTCGGAACCTTGATTTTCTGGTATCCCATGCTTGCACTACTCCGCTGTCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCAt                                                                                                                                                <  2:54752/136‑1 (MQ=255)
                 cGTCGGTCGGAACCTTGATTTTCTGGTATCCCATGCTTGCACTACTCCGCTGTCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCtt                                                                                                                                  >  5:7325/1‑149 (MQ=255)
                   tcggtcggAACCTTGATTTTCTGGTATCCCATGCTTGCACTACTCCGCTGTCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTg                                                                                                                                <  7:38925/149‑1 (MQ=255)
                      gtcggAACCTTGATTTTCTGGTATCCCATGCTTGCACTACTCCGCTGTCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTc                                                                                                                             <  4:114597/149‑1 (MQ=255)
                          gAACCTTGATTTTCTGGTATCCCATGCTTGCACTACTCCGCTGTCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTta                                                                                                                          >  5:132256/1‑148 (MQ=255)
                              cTTGAATTTCTGGTATCCCATGCTTGCACTACTCCGCTGTCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAAt                                                                                                                     >  2:292596/1‑149 (MQ=255)
                                   ttttCTGGTATCCCATGCTTGCACTACTCCGCTGTCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCa                                                                                                                                                 <  5:41475/116‑1 (MQ=255)
                                   ttttCTGGTATCCCAAGCTTGCACTACTCCGCTGTCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCa                                                                                                                                                 >  6:41475/1‑116 (MQ=255)
                                        tGGTATCCCATGCTTGCACTACTCCGCTGTCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGt                                                                                                                                     <  4:189870/123‑1 (MQ=255)
                                        tGGTATCCCATGCTTGCACTACTCCGCTGTCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGt                                                                                                                                     >  3:189870/1‑123 (MQ=255)
                                        tGGTATCCCATGCTTGCACTACTCCGCTGTCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGatat                                                                                                           >  4:145730/1‑149 (MQ=255)
                                          gTATCCCATGCTTGCACTACTCCGCTGTCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATc                                                                                                          <  6:132256/148‑1 (MQ=255)
                                              cccATGCTTGCACTACTCCGCTGTCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACtt                                                                                                      <  2:330082/148‑1 (MQ=255)
                                               ccATGCTTGCACTACTCCGCTGTCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTc                                                                                                                                    >  8:174372/1‑117 (MQ=255)
                                               ccATGCTTGCACTACTCCGCTGTCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTc                                                                                                                                    <  7:174372/117‑1 (MQ=255)
                                               ccATGCTTGCACTACTCCGCTGTCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTgg                                                                                                    >  5:52175/1‑149 (MQ=255)
                                                    cTTGCACTACTCCGCTGTCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTg                                                                                               <  1:292596/149‑1 (MQ=255)
                                                       gCACTACTCCGCTGTCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGc                                                                                              <  3:145730/147‑1 (MQ=255)
                                                         actactCCGCTGTCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGAt                                                                                          <  6:7325/149‑1 (MQ=255)
                                                           tactCCGCTGTCGGGTGTGCTTTGGACATCGTGCGAACCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCAGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACATCGCCTTGATGATTa                                                                                        >  7:127603/1‑149 (MQ=255)
                                                             ctCCGCTGTCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTa                                                                                        <  6:50060/147‑1 (MQ=255)
                                                                         gtgtGGTTTGGACATCGTGCGATCCACTGAGCCTAACCCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGCCCAAATGGTTTg                                                                          <  2:102688/149‑1 (MQ=255)
                                                                               tttGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGCCCAAATGGTTTGGTATAc                                                                    >  1:136534/1‑149 (MQ=255)
                                                                                   gACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTtat                                                                                                                         <  2:71802/92‑1 (MQ=255)
                                                                                   gACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTtat                                                                                                                         >  1:71802/1‑92 (MQ=255)
                                                                                   gACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGCCCAAATGGTTTGGTATACTccg                                                                >  7:279840/1‑149 (MQ=255)
                                                                                   gACATCGTGCGATCCACTGAGCCTAAACCACATATGCCGCCATGAAAGCCATGCGACACCCCCCCGATTTCCTACTTAGTATTTGGAATTATAAATGGGCCGATAAACATTGGCCTTGATGATTa                                                                                        >  6:85068/1‑125 (MQ=255)
                                                                                         gTGCGATCCACTGAGCCTAAACCACATCTGCCTACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGCCCAAATGGTTTGGTATACTCCGCCGCGa                                                          <  1:51603/149‑1 (MQ=255)
                                                                                               tCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGCCCAAATGGTTTGGTATACTCCGCCGCGACCGAAg                                                    <  6:52175/149‑1 (MQ=255)
                                                                                                  aCTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTa                                                                                                                                       <  5:123660/63‑1 (MQ=255)
                                                                                                  aCTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTa                                                                                                                                       >  6:123660/1‑63 (MQ=255)
                                                                                                   cTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCTGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGCCCAAATGGTTTGGTATACTCCGCCGCGACCGAAgg                                                   >  1:122665/1‑146 (MQ=255)
                                                                                                   cTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCTGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGCCCAAATGGTTTGGTATACTCCGCCGCGACCGAAgg                                                   <  2:122665/146‑1 (MQ=255)
                                                                                                        ccTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGCCCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCgg                                           >  3:9081/1‑149 (MQ=255)
                                                                                                              ccACATCTGCCGACCTGCAAGGCATGCGACAACCCGCTGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGCCCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGaa                                     >  5:224349/1‑149 (MQ=255)
                                                                                                              ccACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAAt                                                                                                                     >  8:270034/1‑69 (MQ=255)
                                                                                                              ccACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAAt                                                                                                                     <  7:270034/69‑1 (MQ=255)
                                                                                                              ccACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGCCCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGaa                                     >  7:121822/1‑149 (MQ=255)
                                                                                                              ccACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGCCCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGaa                                     >  3:227781/1‑149 (MQ=255)
                                                                                                                    cTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGCCCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAg                               >  6:101920/1‑149 (MQ=255)
                                                                                                                     tGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGCCCAAATGGTTTGGta                                                                       <  8:239113/108‑1 (MQ=255)
                                                                                                                     tGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGCCCAAATGGTTTGGta                                                                       >  7:239113/1‑108 (MQ=255)
                                                                                                                         gACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGCCCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGaaaa                          >  2:102131/1‑149 (MQ=255)
                                                                                                                          aCCTGCAAGGCATGCGACAACCCGCTGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGCCCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAAt                         <  6:224349/149‑1 (MQ=255)
                                                                                                                            cTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGCCCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGc                       >  4:71635/1‑149 (MQ=255)
                                                                                                                             tGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGCCCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCg                      >  6:325077/1‑149 (MQ=255)
                                                                                                                                aaGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGCCCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGAc                   >  6:300707/1‑149 (MQ=255)
                                                                                                                                 aGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACGTGGCCTTGCTGATTAGCCGAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAAGCCCAGGAAAAGGCGGAcc                  >  8:71262/1‑149 (MQ=255)
                                                                                                                                        cGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGCCCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTa            <  5:101920/148‑1 (MQ=255)
                                                                                                                                         gACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGCCCAAATGGTTTGGTATTCTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAgg          <  1:102131/149‑1 (MQ=255)
                                                                                                                                              cccGCCGCATTGCTGCTTAGTC‑TTGGTATTATAAATGGGCCGATATAAATTGGCCTTGATGATTAGACAAAATGTTTTGTTATAATAACCCGCGACCGAAGGTTAATCGGGGCGAATACCAGGAAAATGAGGACCGCCATAGGACAtt     >  7:228034/1‑147 (MQ=255)
                                                                                                                                               ccgccgCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGCCCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATg     >  8:171319/1‑148 (MQ=255)
                                                                                                                                                 gccgcATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGCCCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATGAAt  <  2:136534/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GACGGTAATTTTGGTGCCGTCGGTCGGAACCTTGATTTTCTGGTATCCCATGCTTGCACTACTCCGCTGTCGGGTGTGGTTTGGACATCGTGCGATCCACTGAGCCTAAACCACATCTGCCGACCTGCAAGGCATGCGACAACCCGCCGCATTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGCCCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATGAAT  >  NC_002947/4521687‑4521980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑