Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,528,353 T→A 9.6% intergenic (+28/‑48) purB → / → PP_4017 adenylosuccinate lyase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,528,3530TA 9.6% 131.7 / 5.8 52intergenic (+28/‑48)purB/PP_4017adenylosuccinate lyase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (21/26);  new base A (2/3);  total (23/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.77e-01

CTGACCTTCATCGACGGCCTGGACATGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCG‑TTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATGCGCGACTACTGGCAGAAGAAGCCGCTGCT  >  NC_002947/4528215‑4528492
                                                                                                                                          |                                                                                                                                            
ctgACCTTCATCGACGGCCTGGACATGCCAGCCGGAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCAGGCCGGGCg                                                                                                                                     <  8:25693/148‑1 (MQ=255)
 tgACCTTCATCGACGGCCTGGACATGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCG‑tt                                                                                                                                  <  6:224232/149‑1 (MQ=255)
 ggACCTTCATATAAGGCCTGGACATGCCATCCGAAGCCAAGGCCGAGCTCAAGCAGCTTACACCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAAAGCATCTAAGCTGATCGTCTCGTACAACGCCCGGCCTTTCCGGGAG‑tt                                                                                                                                  <  4:10012/148‑1 (MQ=255)
     cTTCATCGACGGCCTGGACATGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCG‑TTTTTa                                                                                                                              >  5:190164/1‑149 (MQ=255)
         aTCGACGGCCTGGACATGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCG‑TTTTTATTc                                                                                                                           <  6:81224/148‑1 (MQ=255)
          tCGACGGCCTGGACATGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTg                                                                                                                                             >  7:162833/1‑130 (MQ=255)
          tCGACGGCCTGGACATGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTg                                                                                                                                             <  8:162833/130‑1 (MQ=255)
          tCGACGGCCTGGACATGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCG‑TTTTTATTccc                                                                                                                         >  1:248672/1‑149 (MQ=255)
                  cTGGACATGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCG‑TTTTTATTCCCGTACGaaa                                                                                                                 <  1:76879/149‑1 (MQ=255)
                   tGGACATGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCG‑TTTTTATTCCCGTACGaaaa                                                                                                                >  1:233910/1‑149 (MQ=255)
                        aTGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCG‑TTTTTATTCCCGTACGAAAATTACg                                                                                                           <  7:83489/149‑1 (MQ=255)
                               ccGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCG‑TTTTTATTCCCGTACGAAAATTAAGtttttt                                                                                                     >  1:304068/1‑148 (MQ=255)
                                 gAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGATGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCG‑TTTTTATTCCCGTACGAAAATTACGTTTTTTCaa                                                                                                  >  3:127565/1‑149 (MQ=255)
                                  aaGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCG‑TTTTTATTCCCGTACGAAAATTACGTTTTTTCaa                                                                                                  >  2:271501/1‑148 (MQ=255)
                                      cAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCG‑TTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGt                                                                                             >  6:48540/1‑149 (MQ=255)
                                       aaGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCG‑TTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGtt                                                                                            <  5:11619/149‑1 (MQ=255)
                                        aGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCAGGCCGGGCG‑tt                                                                                                                                  >  5:357917/1‑110 (MQ=255)
                                        aGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCAGGCCGGGCG‑tt                                                                                                                                  <  6:357917/110‑1 (MQ=255)
                                            cGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTTGCGTACAACGCCCGGCCTGGCCGGGCG‑TTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACa                                                                                       <  1:78862/149‑1 (MQ=255)
                                                cTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCG‑TTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGaa                                                                                   >  5:160690/1‑149 (MQ=255)
                                                cTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCG‑TTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGaa                                                                                   <  3:113626/149‑1 (MQ=255)
                                                   aaGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCG‑TTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACAt                                                                                      <  7:283736/143‑1 (MQ=255)
                                                   aaGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCG‑TTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACAt                                                                                      >  8:283736/1‑143 (MQ=255)
                                                    agaagcTTACCCCCGCTACCTACATGGGTAAGGCGGCTGCCCTGGCAAAAGGAATCTATGCTTAGCGTGGCGTAAAACGCCCGGCTTGGCCGGGCGCCTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATcc                                                                                <  1:271501/146‑1 (MQ=255)
                                                      cagcTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCG‑TTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGa                                                                             <  2:233910/149‑1 (MQ=255)
                                                         cTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAGCGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCG‑TTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATAc                                                                          >  6:159845/1‑149 (MQ=255)
                                                             cccccGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCG‑TTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCa                                                                      <  6:190164/149‑1 (MQ=255)
                                                                ccGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCG‑TTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTg                                                                   >  2:277761/1‑149 (MQ=255)
                                                                ccGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCG‑TTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTg                                                                   >  1:252603/1‑149 (MQ=255)
                                                                   ctacctacATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCG‑TTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAg                                                                <  6:244568/149‑1 (MQ=255)
                                                                      cctacATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCG‑TTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAgc                                                               <  5:48540/147‑1 (MQ=255)
                                                                        tacATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTTCAACGCCCGGCCTGGCCGGGCG‑TTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAgctgct                                                           >  2:75234/1‑149 (MQ=255)
                                                                            tCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCG‑TTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAgg                                                                                              <  8:294734/110‑1 (MQ=255)
                                                                              ggTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCG‑TTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTcggcg                                                      <  2:205701/148‑1 (MQ=255)
                                                                              ggTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCG‑TTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTcggcg                                                      <  2:252603/148‑1 (MQ=255)
                                                                                     cgGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCG‑TTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGa                                                                             <  6:54568/118‑1 (MQ=255)
                                                                                     cgGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCG‑TTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGa                                                                             >  5:54568/1‑118 (MQ=255)
                                                                                         aGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCG‑TTTTTATTCCCGTACGAAAATTACGTTTTTTAAATAGGTTGAACATGAATCCTGAAAATCCACTGCAGCTGCTAGGCGGCATCTCGGccc                                          >  4:22621/1‑149 (MQ=255)
                                                                                             cAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCG‑TTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCcgcg                                       >  1:147673/1‑148 (MQ=255)
                                                                                                  cAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCG‑TTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCa                                 <  3:22621/149‑1 (MQ=255)
                                                                                                    aaCGCATCTAAGATGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCG‑TTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATg                               <  4:127565/149‑1 (MQ=255)
                                                                                                       gCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCG‑TTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATgcgc                            <  5:159845/149‑1 (MQ=255)
                                                                                                       gCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCG‑TTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATgcgc                            >  4:130884/1‑149 (MQ=255)
                                                                                                        cATCTTAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCG‑TTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATGcgcg                           <  5:92310/149‑1 (MQ=255)
                                                                                                         aTCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCG‑TTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATGCGCGa                          <  6:160690/149‑1 (MQ=255)
                                                                                                                 tGAGCGTCGCGTACAAAGCCAGGCATGGCCGGGCG‑TATTTATTCCCGTAAGAAAATTACCTTTTTTCAATAGGTTGAAAATGAATCCTGATAATCCACTGCAGCTGCTAGGCGGCATCTCGGCCAGAGAATTCATGCGCGACTACTgg                   >  8:95436/1‑148 (MQ=255)
                                                                                                                      gTCGCGTACAACGCCCGGCCTGGCCGGGCG‑TTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATGCGCGACTACTGGCag                >  6:332048/1‑146 (MQ=255)
                                                                                                                        gaataaaaaCGCCCGGCCAGGCCGGGCG‑TTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATGCGCGACTACTGGCagaag             >  3:289165/8‑147 (MQ=255)
                                                                                                                        gaataaaaaCGCCCGGCCAGGCCGGGCG‑TTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATGCGCGACTACTGGCAgaagaa           <  4:289165/142‑1 (MQ=255)
                                                                                                                            ttcAACGCCCGGCCTGGCCGGGCG‑TTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCTTCTCGGCCCGCGAATTCATGCGCGACTACTGGCAGAAGAAGCCg       <  1:75234/147‑1 (MQ=255)
                                                                                                                             aCAACGCCCGGCCTGGCCGGGCG‑TTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATGCGCGACTACTGGCAGAAGAAGCCgc      >  4:235877/1‑149 (MQ=255)
                                                                                                                                 cGCCCGGCCTGGCCGGGCG‑TTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCTCTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATGCGCGACTACTGGCAGAAGAAGCCgctgct  <  7:211262/149‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                            
CTGACCTTCATCGACGGCCTGGACATGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCG‑TTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATGCGCGACTACTGGCAGAAGAAGCCGCTGCT  >  NC_002947/4528215‑4528492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑