Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,557,029 T→G 13.6% K150Q (AAG→CAG)  gntZ ← 6‑phosphogluconate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,557,0290TG13.6% 150.3 / 17.7 59K150Q (AAG→CAG) gntZ6‑phosphogluconate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (27/24);  new base G (3/5);  total (30/29)
Fisher's exact test for biased strand distribution p-value = 4.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.56e-01

TACTCGATGCCGTTATGCACCATTTTCACGTAGTGCCCGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAGGCCGCGCTTGGCCAGTTCTGCAGCGCGGCGCATGTC  >  NC_002947/4556887‑4557176
                                                                                                                                              |                                                                                                                                                   
tACTCGATGCCGTTATGCACCATTTTCACGTAGTGCCCGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAg                                                                                                                                               >  5:322848/1‑149 (MQ=255)
  cTCGATGCCGTTATGCACCATTTTCACGTAGTGCCCGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGc                                                                                                                                              <  5:149518/148‑1 (MQ=255)
     gATGCCGTTATGCACCATTTTCACGTAGTGCCCGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCgg                                                                                                                                            <  8:77991/147‑1 (MQ=255)
         ccGTTATGCACCATTTTCACGTAGTGCCCGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAg                                                                                                                                      <  4:86976/149‑1 (MQ=255)
           gTTATGCACCATTTTCACGTAGTGCCCGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTGGAACCGCTGTTCAAGgc                                                                                                                                    <  6:204084/149‑1 (MQ=255)
            ttATGCACCATTTTCACGTAGTGCCCGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTGGAACCGCTGTTCAAGgcg                                                                                                                                   <  6:180262/149‑1 (MQ=255)
                 cACCATTTTCACGTAGTGCCCGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCg                                                                                                                               >  4:47026/1‑148 (MQ=255)
                  aCCATTTTCACGTAGTGCCCGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGTCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTg                                                                                                                                                    >  7:236794/1‑126 (MQ=255)
                  aCCATTTTCACGTAGTGCCCGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTg                                                                                                                                                    <  8:236794/126‑1 (MQ=255)
                  aCCATTTTCACGTAGTGCCCGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGaa                                                                                                                             >  5:144076/1‑149 (MQ=255)
                     aTTTTCACGTAGTGCCCGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAacac                                                                                                                          >  3:256836/1‑149 (MQ=255)
                     aTTTTCACGTAGTGCCCGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAacac                                                                                                                          <  6:322848/149‑1 (MQ=255)
                     aTTTTCACGTAGTGCCCGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTGGAACCGCTGTTCAAGGCGCtggcgccg                                                                                                                          <  6:42380/149‑8 (MQ=39)
                        ttCACGTAGTGCCCGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTc                                                                                                                       >  2:202824/1‑149 (MQ=255)
                            cGTAGTGCCCGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACACCGGTTCCAGGCGCTCGAACACGTCCttt                                                                                                                   <  1:202824/149‑1 (MQ=255)
                             gTAGTGCCCGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCtttt                                                                                                                  >  6:278342/1‑149 (MQ=255)
                                gTGCCCGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGTGCTCGAACACGTc                                                                                                                       >  7:163232/1‑141 (MQ=255)
                                gTGCCCGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGTGCTCGAACACGTc                                                                                                                       <  8:163232/141‑1 (MQ=255)
                                 tGCCCGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTcgcc                                                                                                              >  3:125558/1‑149 (MQ=255)
                                   cccGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTcgccgc                                                                                                            <  5:183298/149‑1 (MQ=255)
                                       gcgcCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAAGAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGat                                                                                                        >  5:311374/1‑149 (MQ=255)
                                         gcCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGatca                                                                                                      >  5:148141/1‑149 (MQ=255)
                                          cccGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGatcat                                                                                                     <  5:183339/149‑1 (MQ=255)
                                           ccGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTGGAACCGCTGTTCAAGGCGCtg                                                                                                                                >  6:81596/1‑120 (MQ=255)
                                           ccGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTGGAACCGCTGTTCAAGGCGCtg                                                                                                                                <  5:81596/121‑2 (MQ=255)
                                              ttGGACCTGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACTCGTCCTTTTCGCCGCCGATCTTCATg                                                                                                 <  7:116001/148‑1 (MQ=255)
                                                ggACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATCGCAACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGACCTTTTCGCCGCCGATCATCATg                                                                                                 >  8:116001/1‑147 (MQ=255)
                                                      gCATGGATGTACCCGTGCTCGGCAAGCTGGTGCTAGCCGCTGCGGCCATCGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACCTACTTTTCGCCTCCGATCATCATGCAGTAGcc                                                                                         >  8:94258/1‑149 (MQ=255)
                                                        aTGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCcgc                                                                                       >  6:36947/1‑149 (MQ=255)
                                                             tGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTc                                                                                                                                >  3:149873/1‑103 (MQ=255)
                                                             tGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTc                                                                                                                                <  4:149873/103‑1 (MQ=255)
                                                             tGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGAtcatca                                                                                                   <  7:316635/132‑1 (MQ=255)
                                                             tGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGAtcatca                                                                                                   >  8:316635/1‑132 (MQ=255)
                                                                  ccGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGcc                                                                             >  4:106139/1‑149 (MQ=255)
                                                                     tGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCca                                                                          <  6:144076/149‑1 (MQ=255)
                                                                          ggCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTGGAACCGCTGTTCAAg                                                                                                                                      <  3:137164/84‑1 (MQ=38)
                                                                          ggCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTGGAACCGCTGTTCAAg                                                                                                                                      >  4:137164/1‑84 (MQ=38)
                                                                             aCGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACAcgccgc                                                                  <  4:125558/149‑1 (MQ=255)
                                                                                   gTGCTGTCCGCTTCGGCCTTGTGTACGCGTGATGTCGCAGACGCCAGGCGCCAGAGCCTTGTACAGCGGTTCTAGTTTCTGTATCATGTCCTTTTGGCCTCCGATCTTCATTCTTTAGCCGCGTTGCATGCCCCACACGCCGCCGGaa                                                             <  4:256836/148‑1 (MQ=255)
                                                                                       tCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCTGTCCa                                                                                 <  5:58931/124‑1 (MQ=255)
                                                                                       tCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCa                                                                                 >  6:58931/1‑124 (MQ=255)
                                                                                       tCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTccc                                                        >  1:339761/1‑149 (MQ=255)
                                                                                        cgccgcTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCg                                                       >  4:12726/1‑149 (MQ=255)
                                                                                         gccgcTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGa                                                      >  6:350146/1‑149 (MQ=255)
                                                                                                  gCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGa                                              >  4:109390/1‑148 (MQ=255)
                                                                                                    cATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATaa                                           >  5:267982/1‑149 (MQ=255)
                                                                                                        ggTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCca                                                                          >  8:42622/1‑114 (MQ=255)
                                                                                                        ggTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCca                                                                          <  7:42622/114‑1 (MQ=255)
                                                                                                        ggTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATg                                         >  2:338189/1‑147 (MQ=255)
                                                                                                        ggTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCa                                       <  6:267982/149‑1 (MQ=255)
                                                                                                                 gATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAGGCCGCGCt                              >  6:267742/1‑149 (MQ=255)
                                                                                                                     tCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAGGCCGCGCTTGGc                          >  8:319535/1‑149 (MQ=255)
                                                                                                                          cACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAGGCCGCGCTTGGCCAGtt                     <  5:350146/149‑1 (MQ=255)
                                                                                                                             gCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAGGCCGCGCTTGGCCAGTTCTg                  <  6:148141/149‑1 (MQ=255)
                                                                                                                                    gcCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAGGCCGCGCTTGGCCAGTTCTGCAGcgcg           <  1:338189/149‑1 (MQ=255)
                                                                                                                                      cAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAGGCCGCGCTTGGCCAGTTCTGCAGCgcggc         <  2:339761/149‑1 (MQ=255)
                                                                                                                                         cgcCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGTCCACACACGCCGCCTGAAGTCCCGACGTCCAGATAATGCAGTCCGCGCTTGGCCAGTTCTGCAGCTCGGCGCa      <  7:94258/149‑1 (MQ=255)
                                                                                                                                          gcCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAGGCCGCGCTTGGCCAGTTCTGCAGCGCGGCGCAt     <  5:36947/149‑1 (MQ=255)
                                                                                                                                          gcCTGGAACCGCTGTTCAAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCAGCCGGAAGTCCCGACGTCCAGATAATGCAGGACGCGCTTGGCCATTTCTGCAGCGCGGCGCAt     >  7:318318/1‑149 (MQ=255)
                                                                                                                                             ttGAACAGTGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAGGCCGCGCTTGGCCAGTTCTGCAGCGCGGCGCATgtc  >  7:212237/1‑149 (MQ=255)
                                                                                                                                             ttGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCa                                       >  8:106031/1‑112 (MQ=255)
                                                                                                                                             ttGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCa                                       <  7:106031/112‑1 (MQ=255)
                                                                                                                                             ttGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAGGCCGCGCTTGGCCAGTTCTGCAGCGCGGCGCATgtc  >  3:194674/1‑149 (MQ=255)
                                                                                                                                             ttGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAGGCCGCGCTTGGCCAGTTCTGCAGCGCGGCGCATgtc  >  4:85281/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                   
TACTCGATGCCGTTATGCACCATTTTCACGTAGTGCCCGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAGGCCGCGCTTGGCCAGTTCTGCAGCGCGGCGCATGTC  >  NC_002947/4556887‑4557176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑