Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,587,698 G→C 9.6% S39S (TCC→TCG) ‡ PP_4063 ← long‑chain fatty acid‑‑CoA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,587,6980GC 9.6% 148.5 / 7.0 52S39S (TCC→TCG) ‡PP_4063long‑chain fatty acid‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base G (23/24);  new base C (3/2);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.70e-01

CCAGCTGCAAGATGCACCACTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTTGTGGTTGTTTTG  >  NC_002947/4587550‑4587840
                                                                                                                                                    |                                                                                                                                              
ccAGCTGCAAGATGCACCACTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCgg                                                                                                                                                <  6:368416/149‑1 (MQ=255)
    cTGCAAGATGCACCACTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGaca                                                                                                                                            >  4:243350/1‑149 (MQ=255)
     tGCAAGATGCACCACTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGccac                                                                                                                                           >  3:147864/1‑149 (MQ=255)
         aatctcaccacTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGTCCCGCCACCAgg                                                                                                                                       <  7:93109/144‑1 (MQ=255)
         aGATGCACCACTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGTCCCGCCACCAgg                                                                                                                                       <  3:79212/149‑1 (MQ=255)
         aGATGCACCACTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAgg                                                                                                                                       <  4:147864/149‑1 (MQ=255)
               accacTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGTCCCGCCACCAgg                                                                                                                                       >  8:93109/1‑143 (MQ=255)
                   cTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGt                                                                                                                             <  8:186776/149‑1 (MQ=255)
                    tGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACAcc                                                                                                                                          <  1:278090/135‑1 (MQ=255)
                    tGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACAcc                                                                                                                                          >  2:278090/1‑135 (MQ=255)
                      ggCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCg                                                                                                                           >  8:75616/1‑148 (MQ=255)
                      ggCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGc                                                                                                                          <  8:118466/149‑1 (MQ=255)
                         gcAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCaa                                                                                                                        >  8:34000/1‑148 (MQ=255)
                               gggCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCggg                                                                                                                 >  7:158168/1‑149 (MQ=255)
                                             cAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATc                                                                                                    >  3:64153/1‑148 (MQ=255)
                                                  cGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGc                                                                                              <  2:153822/149‑1 (MQ=255)
                                                                cACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCt                                                                                >  8:153438/1‑149 (MQ=255)
                                                                 aCGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCt                                                                                <  1:329815/148‑1 (MQ=255)
                                                                 aCGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTg                                                                               >  8:166686/1‑149 (MQ=255)
                                                                 aCGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTg                                                                               <  4:64153/149‑1 (MQ=255)
                                                                   gCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTggg                                                                             >  7:12938/1‑149 (MQ=255)
                                                                     ccAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGt                                                                            >  5:207935/1‑148 (MQ=255)
                                                                       aGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAg                                                                         >  5:275183/1‑149 (MQ=255)
                                                                            gATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGt                                                                                  >  7:314827/1‑135 (MQ=255)
                                                                            gATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGt                                                                                  <  8:314827/135‑1 (MQ=255)
                                                                              tCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGggt                                                                  <  7:34000/149‑1 (MQ=255)
                                                                                   gcgcGGGCGTATATTTCGACGTGTTCGGCCATGTGCCGCCAGCTGTAACGGAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGc                                                                                                       <  4:99072/107‑1 (MQ=255)
                                                                                   gcgcGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGggtcggtc                                                             <  7:166686/149‑1 (MQ=255)
                                                                                   gcgcGGGCGTATATTTCGACCTGTTCGGCCAACTGACGCCACCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGCTGGc                                                                                                       >  3:99072/1‑107 (MQ=255)
                                                                                           gTATATTTCGACCTGTACGGACAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACCCCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGGCGGTCCTGGCGAc                                                     <  8:308444/149‑1 (MQ=255)
                                                                                            tataTTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCATGCCCTGGTGGCGGGACACCAGGGACTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTc                                                                           >  1:1999/1‑126 (MQ=255)
                                                                                             atatTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGt                                                                                  >  1:166292/1‑118 (MQ=255)
                                                                                             atatTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGt                                                                                  <  2:166292/118‑1 (MQ=255)
                                                                                             atatTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGggtcgg                                                               >  1:263185/1‑137 (MQ=255)
                                                                                             atatTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGggtcgg                                                               <  2:263185/137‑1 (MQ=255)
                                                                                              tatTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACcgc                                                  <  7:153438/149‑1 (MQ=255)
                                                                                                     aCCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGta                                             >  5:271742/1‑147 (MQ=255)
                                                                                                      ccTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAg                                          >  1:169981/1‑149 (MQ=255)
                                                                                                          ttCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCg                                      <  1:216914/149‑1 (MQ=255)
                                                                                                             ggCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGtt                                   <  8:158168/149‑1 (MQ=255)
                                                                                                                cAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGAc                                <  2:169981/149‑1 (MQ=255)
                                                                                                                      ccgccAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATgg                          <  6:275183/149‑1 (MQ=255)
                                                                                                                         ccAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTgg                                                                              >  2:203664/1‑94 (MQ=255)
                                                                                                                         ccAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTgg                                                                              <  1:203664/94‑1 (MQ=255)
                                                                                                                              tGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCct                  >  1:136503/1‑149 (MQ=255)
                                                                                                                                 aaCGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTGGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCcttc                >  7:197314/1‑148 (MQ=255)
                                                                                                                                 aaCGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCcttc                <  8:197314/148‑1 (MQ=255)
                                                                                                                                   cGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGtt                                   >  4:187992/1‑127 (MQ=255)
                                                                                                                                     gaGGCCCTGGTGTCCCGCCACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTtgtg           >  4:113186/2‑149 (MQ=255)
                                                                                                                                       ggCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTTGTGgt         <  2:136503/149‑1 (MQ=255)
                                                                                                                                        gCCCTGGTGTCCCGCCACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTTGTGgtt        >  6:13931/1‑149 (MQ=255)
                                                                                                                                           cTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTTGTGgttgtt     <  8:12938/149‑1 (MQ=255)
                                                                                                                                              gtggCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTTGTGGTTGTTTTg  <  6:207935/149‑1 (MQ=255)
                                                                                                                                                   gggACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTg                                                          <  8:202275/88‑1 (MQ=255)
                                                                                                                                                   gggACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTg                                                          >  7:202275/1‑88 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
CCAGCTGCAAGATGCACCACTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTTGTGGTTGTTTTG  >  NC_002947/4587550‑4587840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑