Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,587,700 A→C 9.1% S39A (TCC→GCC) ‡ PP_4063 ← long‑chain fatty acid‑‑CoA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,587,7000AC 9.1% 161.5 / 7.3 55S39A (TCC→GCC) ‡PP_4063long‑chain fatty acid‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base A (24/26);  new base C (3/2);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

CTGCAAGATGCACCACTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTTGTGGTTGTTTTGGGCACGG  >  NC_002947/4587554‑4587847
                                                                                                                                                  |                                                                                                                                                   
cTGCAAGATGCACCACTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGaca                                                                                                                                                   >  4:243350/1‑149 (MQ=255)
 tGCAAGATGCACCACTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGccac                                                                                                                                                  >  3:147864/1‑149 (MQ=255)
     aatctcaccacTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGTCCCGCCACCAgg                                                                                                                                              <  7:93109/144‑1 (MQ=255)
     aGATGCACCACTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGTCCCGCCACCAgg                                                                                                                                              <  3:79212/149‑1 (MQ=255)
     aGATGCACCACTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAgg                                                                                                                                              <  4:147864/149‑1 (MQ=255)
           accacTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGTCCCGCCACCAgg                                                                                                                                              >  8:93109/1‑143 (MQ=255)
               cTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGt                                                                                                                                    <  8:186776/149‑1 (MQ=255)
                tGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACAcc                                                                                                                                                 <  1:278090/135‑1 (MQ=255)
                tGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACAcc                                                                                                                                                 >  2:278090/1‑135 (MQ=255)
                  ggCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCg                                                                                                                                  >  8:75616/1‑148 (MQ=255)
                  ggCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGc                                                                                                                                 <  8:118466/149‑1 (MQ=255)
                     gcAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCaa                                                                                                                               >  8:34000/1‑148 (MQ=255)
                           gggCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCggg                                                                                                                        >  7:158168/1‑149 (MQ=255)
                                         cAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATc                                                                                                           >  3:64153/1‑148 (MQ=255)
                                              cGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGc                                                                                                     <  2:153822/149‑1 (MQ=255)
                                                            cACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCt                                                                                       >  8:153438/1‑149 (MQ=255)
                                                             aCGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCt                                                                                       <  1:329815/148‑1 (MQ=255)
                                                             aCGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTg                                                                                      >  8:166686/1‑149 (MQ=255)
                                                             aCGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTg                                                                                      <  4:64153/149‑1 (MQ=255)
                                                               gCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTggg                                                                                    >  7:12938/1‑149 (MQ=255)
                                                                 ccAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGt                                                                                   >  5:207935/1‑148 (MQ=255)
                                                                   aGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAg                                                                                >  5:275183/1‑149 (MQ=255)
                                                                        gATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGt                                                                                         <  8:314827/135‑1 (MQ=255)
                                                                        gATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGt                                                                                         >  7:314827/1‑135 (MQ=255)
                                                                          tCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGggt                                                                         <  7:34000/149‑1 (MQ=255)
                                                                               gcgcGGGCGTATATTTCGACGTGTTCGGCCATGTGCCGCCAGCTGTAACGGAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGc                                                                                                              <  4:99072/107‑1 (MQ=255)
                                                                               gcgcGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGggtcggtc                                                                    <  7:166686/149‑1 (MQ=255)
                                                                               gcgcGGGCGTATATTTCGACCTGTTCGGCCAACTGACGCCACCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGCTGGc                                                                                                              >  3:99072/1‑107 (MQ=255)
                                                                                       gTATATTTCGACCTGTACGGACAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACCCCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGGCGGTCCTGGCGAc                                                            <  8:308444/149‑1 (MQ=255)
                                                                                        tataTTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCATGCCCTGGTGGCGGGACACCAGGGACTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTc                                                                                  >  1:1999/1‑126 (MQ=255)
                                                                                         atatTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGt                                                                                         >  1:166292/1‑118 (MQ=255)
                                                                                         atatTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGt                                                                                         <  2:166292/118‑1 (MQ=255)
                                                                                         atatTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGggtcgg                                                                      >  1:263185/1‑137 (MQ=255)
                                                                                         atatTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGggtcgg                                                                      <  2:263185/137‑1 (MQ=255)
                                                                                          tatTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACcgc                                                         <  7:153438/149‑1 (MQ=255)
                                                                                                 aCCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGta                                                    >  5:271742/1‑147 (MQ=255)
                                                                                                  ccTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAg                                                 >  1:169981/1‑149 (MQ=255)
                                                                                                      ttCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCg                                             <  1:216914/149‑1 (MQ=255)
                                                                                                         ggCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGtt                                          <  8:158168/149‑1 (MQ=255)
                                                                                                            cAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGAc                                       <  2:169981/149‑1 (MQ=255)
                                                                                                                  ccgccAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATgg                                 <  6:275183/149‑1 (MQ=255)
                                                                                                                     ccAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTgg                                                                                     >  2:203664/1‑94 (MQ=255)
                                                                                                                     ccAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTgg                                                                                     <  1:203664/94‑1 (MQ=255)
                                                                                                                          tGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCct                         >  1:136503/1‑149 (MQ=255)
                                                                                                                             aaCGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTGGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCcttc                       >  7:197314/1‑148 (MQ=255)
                                                                                                                             aaCGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCcttc                       <  8:197314/148‑1 (MQ=255)
                                                                                                                               cGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGtt                                          >  4:187992/1‑127 (MQ=255)
                                                                                                                                 gaGGCCCTGGTGTCCCGCCACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTtgtg                  >  4:113186/2‑149 (MQ=255)
                                                                                                                                   ggCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTTGTGgt                <  2:136503/149‑1 (MQ=255)
                                                                                                                                    gCCCTGGTGTCCCGCCACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTTGTGgtt               >  6:13931/1‑149 (MQ=255)
                                                                                                                                       cTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTTGTGgttgtt            <  8:12938/149‑1 (MQ=255)
                                                                                                                                          gtggCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTTGTGGTTGTTTTg         <  6:207935/149‑1 (MQ=255)
                                                                                                                                               gggACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTg                                                                 <  8:202275/88‑1 (MQ=255)
                                                                                                                                               gggACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTg                                                                 >  7:202275/1‑88 (MQ=255)
                                                                                                                                                 gACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGggt                                                                         <  1:21702/78‑1 (MQ=255)
                                                                                                                                                 gACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGggt                                                                         >  2:21702/1‑78 (MQ=255)
                                                                                                                                                 gACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTTGTGGTTGTTTTGGGCACgg  >  8:331664/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CTGCAAGATGCACCACTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTTGTGGTTGTTTTGGGCACGG  >  NC_002947/4587554‑4587847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑