Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	345,707	C→A	5.7%	L713L (CTG→CTT)	PP_0287 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	345,707	0	C	A	5.7%	217.7 / 3.4	70	L713L (CTG→CTT)	PP_0287	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (31/34);  new base A (2/2);  total (33/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.69e-01

CAGTCCAGCACTGCGCTGGAGAACTGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAGGCCGGGTCCGGCATGGCACGCTG  >  NC_002947/345565‑345853                                                                                                                                               |                                                                                                                                                   cAGTCCAGCACTGAGCTGGAGAACTGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTAAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCAGGCCAGATTg                                                                                                                                              >  4:302062/1‑149 (MQ=255)      cAGCACTGCGCTGGAGAACTGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTggcggc                                                                                                                                         <  5:9644/149‑1 (MQ=255)      cAGCACTGCGCTGGAGAACTGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTggcg                                                                                                                                           <  3:38938/147‑1 (MQ=255)       aGCACTGCGCTGGAGAACTGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTggcggc                                                                                                                                         >  7:264926/1‑148 (MQ=255)                 tGGAGAACTGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGcc                                                                                                                                >  1:179879/1‑147 (MQ=255)                   gagaACTGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCa                                                                                                                              <  1:254094/147‑1 (MQ=255)                        cTGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGtt                                                                                                                       <  6:255342/149‑1 (MQ=255)                           cTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTg                                                                                                                    >  7:141083/1‑149 (MQ=255)                              ggggCTCATCGGTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTc                                                                                                                 >  8:82042/1‑149 (MQ=255)                              ggggCTCATCGGTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTc                                                                                                                 >  8:70683/1‑149 (MQ=255)                                         gTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAgg                                                                                                      >  2:257307/1‑149 (MQ=255)                                            gAACAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCt                                                                                                   >  7:173173/1‑149 (MQ=255)                                              aCAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCttt                                                                                                 <  7:70683/149‑1 (MQ=255)                                                aGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTgc                                                                                               >  2:14009/1‑149 (MQ=255)                                                  ccccTTGAGCGAGTCTATCACATCCGGCCTCCCTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCACCCTAGCCCCGGCCGTCCTg                                                                                                                    >  6:264047/1‑126 (MQ=255)                                                     cTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGcc                                                                                            >  4:135927/1‑147 (MQ=255)                                                        gcgcgcGGCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGGTTATCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAg                                                                                         <  8:19645/145‑1 (MQ=255)                                                         aGCGCGTCTTTCAGTTCCGGGCGCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTc                                                                                      <  8:329368/149‑1 (MQ=255)                                                          gcgcGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTc                                                                                      >  4:226599/1‑148 (MQ=255)                                                           cgcgTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGGCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCa                                                                                    <  1:257307/149‑1 (MQ=255)                                                           cgcgTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTcc                                                                                     <  8:141083/148‑1 (MQ=255)                                                             cgTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGc                                                                                  >  2:310050/1‑149 (MQ=255)                                                                 tttCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGAc                                                                              >  3:1398/1‑149 (MQ=255)                                                                   tCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGa                                                                               <  3:302062/146‑1 (MQ=255)                                                                     aGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACg                                                                          <  8:264926/149‑1 (MQ=255)                                                                       ttCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGc                                                                        <  8:173173/149‑1 (MQ=255)                                                                             gCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAgcg                                                                    >  3:237247/1‑147 (MQ=255)                                                                                 aCTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAgg                                                                <  7:258295/147‑1 (MQ=255)                                                                                   tttGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGc                                                                                  >  7:149377/1‑127 (MQ=255)                                                                                   tttGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGc                                                                                  <  8:149377/127‑1 (MQ=255)                                                                                   tttGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGa                                                            >  1:140333/1‑149 (MQ=255)                                                                                          ccAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCaa                                                      >  2:50156/1‑148 (MQ=255)                                                                                           cAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAaca                                                    >  6:4621/1‑149 (MQ=255)                                                                                             aGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACAcc                                                  >  2:230256/1‑149 (MQ=255)                                                                                                     gTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAAc                                          >  2:349095/1‑149 (MQ=255)                                                                                                      tCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGc                                                                                  >  1:110800/1‑108 (MQ=255)                                                                                                      tCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGc                                                                                  <  2:110800/108‑1 (MQ=255)                                                                                                        aaGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAAcgtt                                       <  7:82042/149‑1 (MQ=255)                                                                                                        aaGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAAcgtt                                       <  3:135927/149‑1 (MQ=255)                                                                                                        aaGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAAcgtt                                       >  3:117483/1‑149 (MQ=255)                                                                                                        aaGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAAcgt                                        <  1:310050/148‑1 (MQ=255)                                                                                                         aGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAAcgttc                                      >  8:83198/1‑149 (MQ=255)                                                                                                           tttttCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAAcgttcgt                                    >  6:292207/1‑149 (MQ=255)                                                                                                             tttCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAAcgttcgtt                                   <  5:130325/148‑1 (MQ=255)                                                                                                                cATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGAc                                <  2:179879/148‑1 (MQ=255)                                                                                                                 aTCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCt                              <  5:4621/149‑1 (MQ=255)                                                                                                                 aTCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCt                              <  2:140333/149‑1 (MQ=255)                                                                                                                 aTCGATCTTGTATTTCAGGCGGTTGCCCGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCACGCATTTGCGCCCACTTACAGCGCACCACGGCAGAGCATCGGGACTTCAACACACACATAACGAACGTAGACCt                              >  8:101678/1‑149 (MQ=255)                                                                                                                  tCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTg                             >  5:138963/1‑149 (MQ=255)                                                                                                                  tCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTG‑CCAGGGCGTTGATGTCGAGGCCGGAGGCCTTTTCCCCCAATTGCCTCCGCACACGGCAGGGCAGGGGGCAATGAACACCCACATAACGTTAGTTTACCTgg                            >  5:264992/1‑149 (MQ=255)                                                                                                                    gATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTgg                            <  4:237247/148‑1 (MQ=255)                                                                                                                          gTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCa                          <  7:101678/144‑1 (MQ=255)                                                                                                                          gTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAgg                        <  4:117483/146‑1 (MQ=255)                                                                                                                          gTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAg                         <  1:230256/145‑1 (MQ=255)                                                                                                                          gTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAGGcc                      <  1:50156/148‑1 (MQ=255)                                                                                                                            cTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAaca                                                    <  1:357376/116‑1 (MQ=255)                                                                                                                            cTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAaca                                                    >  2:357376/1‑116 (MQ=255)                                                                                                                               tctgggggTTGCGGGCATGCTTGGGGGAAACCTTG‑CCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAGGCCGGGTcc                <  6:264992/142‑1 (MQ=255)                                                                                                                                 aGGCGGTTGCCGGCGAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCATGCCTTTGCGCCGAGTTCGAGCGGACCACGGCAGCGCAGTGTGACTTCAACACCCACATAACGTTCGTTTACCCGGCCTGCCAGGTcaa               <  3:265/148‑3 (MQ=255)                                                                                                                                  ggCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAGGCCGGGTCCgg              >  7:48418/1‑148 (MQ=255)                                                                                                                                   aaggTTGCCGCCAAGCTGGCCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAGGCCGGGTCCGGCa            >  1:195792/3‑149 (MQ=255)                                                                                                                                     ggTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAGGCCGGGTCCGGCATg          <  3:225959/149‑1 (MQ=255)                                                                                                                                     ggTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAGGCCGGGTCCGGCATg          <  8:48418/149‑1 (MQ=255)                                                                                                                                     ggTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAGGCCGGGTCCGGCATg          <  4:1398/149‑1 (MQ=255)                                                                                                                                        tGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAGGCCGGGTCCGGCAtagtc       >  8:341041/1‑145 (MQ=255)                                                                                                                                         gccgccAAGCTGGCCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAAcgttcgt                                    <  3:67948/119‑1 (MQ=255)                                                                                                                                         gccgccAAGCTGGCCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAAcgttcgt                                    >  4:67948/1‑119 (MQ=255)                                                                                                                                         gCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGCCATGCCGGACCCGGCCTGCCa       <  1:349095/148‑1 (MQ=255)                                                                                                                                             gCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAGGCCGGGTCCGGCATGGCACGCTg  <  5:292207/149‑1 (MQ=255)                                                                                                                                             gCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAGGCCGGGTCCGGCATGGCACGCTg  <  1:14009/149‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                   CAGTCCAGCACTGCGCTGGAGAACTGCTCGGGGCTCATCGGTTGAACAGCCCCTTGAGCGCGTCTTTCAGTTCCGGGCTCACTTTGTCTCCAAGTTTTTCGTCAAGTTTTTCATCGATCTTGTCTTTCAGGCGGTTGCCGGCCAGCTTGGCGGCAACCTTGCCCAGGCCGTTCTGGTCGAGGCGGCAGGCCTTTGCGCCGAGTTCCAGCGGACCACGGCAGCGCAGGGGGACTTCAACACCCACATAACGTTCGTTGACCTGGCAGGCCGGGTCCGGCATGGCACGCTG  >  NC_002947/345565‑345853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑