Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,612,253	C→G	5.9%	intergenic (+1215/‑892)	hcpC‑II → / → PP_4084	hemolysin‑coregulated protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,612,253	0	C	G	5.9%	215.0 / 3.4	68	intergenic (+1215/‑892)	hcpC‑II/PP_4084	hemolysin‑coregulated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (34/30);  new base G (2/2);  total (36/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

GACCTCAATGCGCATTGGCGGGTCAACACGGTTACCCATAAAGGCCACCAGTTCACCAGCCTGCAGGAAGAAGCCGCCGGCGCCGATGTAAGCACGCGCTACGAGCAGACCGCAGTGCTGGTCCCCGGTCGGACCGAATGGCGCCCTGCGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCACCGTCGTCGGCCCGCCTGGCGAGGAAATCTACTGCGATGAATGGGGCCGGGTAAAAGTCAGTTTCCCCTGGGACCGCGAAAGCCAGAACAACGAGTTCAGT  >  NC_002947/4612108‑4612398                                                                                                                                                  |                                                                                                                                                  gacCTCAATGCGCATTGGCGGGTCAACACGGTTACCCATAAAGGCCACCAGTTCACCAGCCTGCAGGAAGAAGCCGCCGGCGCCGATGTAAGCACGCGCTACGAGCAGACCGCAGTGCTGGTCCCCGGTCGGACCGAATGGCGCCCTgc                                                                                                                                                >  2:263270/1‑149 (MQ=255)    cTCAATGCGCATTGGCGGGTCAACACGGTTACCCATAAAGGCCACCAGTTCACCAGCCTGCAGGAAGAAGCCGCCGGCGCCGATGTAAGCACGCGCTACGAGCAGACCGCAGTGCTGGTCCCCGGTCGGACCGAATGGCGCCCTgcgc                                                                                                                                              >  5:218640/1‑148 (MQ=255)      cAATGCGCATTGGCGGGTCAACACGGTTACCCATAAAGGCCACCAGTTCACCAGCCTGCAGGAAGAAGCCGCCGGCGCCGATGTAAGCACGCGCTACGAGCAGACCGCAGTGCTGGTCCCCGGTCGGACCGAATGGCGCCCTGCGCCAt                                                                                                                                           >  1:228307/1‑149 (MQ=255)      cAATGCGCATTGGCGGGTCAACACGGTTACCCATAAAGGCCACCAGTTCACCAGCCTGCAGGAAGAAGCCGCCGGCGCCGATGTAAGCACGCGCTACGAGCAGACCGCAGTGCTGGTCCCCGGTCGGACCGAATGGCGCAGGGCGCCAt                                                                                                                                           >  5:9831/1‑149 (MQ=255)        aTGCGCATTGGCGGGTCAACACGGTTACCCATAAAGGCCACCAGTTCACCAGCCTGCAGGAAGAAGCCGCCGGCGCCGATGTAAGCACGCGCTACGAGCAGACCGCAGTGCTGGTCCCCGGTCGGACCGAATGGCGCAGGGCGCCAttc                                                                                                                                         <  6:9831/149‑2 (MQ=255)           cgcATTGGCGGTTCAACACGGTTACCCATAAATGCCACCAGTTCACCTGCCTGCAGTAAGAAGCCGCCGGCGCCGATGTAAGCACGCGCTACGAGCAGAGGGCAGTGCTGGTCCCCGTTGGGAGCGATTGGCGCCGTGCGCCATTGaaa                                                                                                                                      <  4:315900/149‑1 (MQ=255)           cgcATTGGCGGGTCAACACGGTTACCCATAAAGGCCACCAGTTCACCAGCCTGCAGGAAGAAGCCGCCGGCGCCGATGTAAGCACGCGCTACGAGCAGACCGCAGTGCTGGTCCCCGGTCGGACCGAATGGCGCCCTGCGCCATTGaa                                                                                                                                       <  5:253542/148‑1 (MQ=255)                    gggTCAACACGGTTACCCATAAAGGCCACCAGTTCACCAGCCTGCAGGAAGAAGCCGCCGGCGCCGATGTAAGCACGCGCTACGAGCAGACCGCAGTGCTGGTCCTCGGTCGGACCGAATGGCGCCCTGCGCCATTGAAAAAACCACGa                                                                                                                             <  1:69449/149‑1 (MQ=255)                    gggTCAACACGGTTACCCATAAAGGCCACCAGTTCACCAGCCTGCAGGAAGAAGCCGCCGGCGCCGATGTAAGCACGCGCTACGAGCAGACCGCAGTGCTGGTCCCCGGTCGGACCGAATGGCGCCCTGCGCCATTGAAAAAACCACg                                                                                                                              >  5:161377/1‑148 (MQ=255)                    gggTCAACACGGTTACCCATAAAGGCCACCAGTTCACCAGCCTGCAGGAAGAAGCCGCCGGCGCCGATGTAAGCACGCGCTACGAGCAGACCGCAGTGATGGTCCCCGGTCGGACCGAATGGCGCCCTGCGCCATTGAAAAAACCAc                                                                                                                               <  4:75563/147‑1 (MQ=255)                    gggTCAACAAGGTTACCCATAAAGGCCACCAGTTCACCAGCCTGCAGGAAGAAGCCGCCTGCGCCGATGTAAGCACGCGCTACGAGCAGACCGCAGTGCTGGTCCCCGGTCGGACCGAATGGCGCCCTGCGCCATTGAAAAAACCAc                                                                                                                               >  3:75563/1‑147 (MQ=255)                         aaCACGGTTACCCATAAAGGCCACCAGTTCACCAGCCTGCAGGAAGAAGCCGCCGGCGCCGATGTAAGCACGCGCTACGAGCAGACCGCAGTGCTGGTCCCCGGTCGGACCGAATGGCGCCCTGCGCCATTGAAAAAACCACGAATCGa                                                                                                                        >  5:298014/1‑149 (MQ=255)                                    ccATAAAGGCCACCAGTTCACCAGCCTGCAGGAAGAAGCCGCCGGCGCCGATGTAAGCACGCGCTACGAGCAGACCGAAGTGCTGGTCCCCGGTCGGACCGAATGCCGCCCTGCGCCATTGAAAAAACCACGAATCGACGGGCCGcaca                                                                                                             >  8:155592/1‑149 (MQ=255)                                    ccATAAAGGCCACCAGTTCACCAGCCTGCAGGAAGAAGCCGCCGGCGCCGATGTAAGCACGCGCTACAAGCAGACCGCAGTGCTGGTCCCCGGTCGGACCGAATGGCGCCCTGCGCCATTGAAAAAACCACGAATCGACGGGCCGcaca                                                                                                             >  5:14197/1‑149 (MQ=255)                                          aGGCCACCAGTTCACCAGCCTGCAGGAAGAAGCCGCCGGCGCCGATGTAAGCACGCGCTACGAGCAGACCGCAGTGCTGGTCCCCGGTCGGACCGAATGGCGCCCTGCGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCa                                                                                                       <  2:220528/149‑1 (MQ=255)                                           ggCCACCAGTTCACCAGCCTGCAGGAAGAAGCCGCCGGCGCCGATGTAAGCACGCGCTACGAGCAGACCGCAGTGCTGGTCCCCGGTCGGACCGAATGGCGCCCTGCGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCAc                                                                                                      <  6:298014/149‑1 (MQ=255)                                               accaGTTCACCAGCCTGCAGGAAGAAGCCGCCGGCGCCGATGTAAGCACGCGCTACGAGCAGACCGCAGTGCTGGTCCCCGGTCGGACCGAATGGCGCCCTGCGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCACcgtc                                                                                                  <  6:218640/149‑1 (MQ=255)                                                    ttCACCAGCCTGCAGGAAGAAGCCGCCGGCGCCGATGTAAGCACGCGCTACGAGCAGACCGCAGTGCTGGTCCCCGGTCGGACCGAATGGCGCCCTGCGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCACCGTCGTCgg                                                                                             <  6:175528/149‑1 (MQ=255)                                                       aCCAGCCTGCAGGAAGAAGCCGCCGGCGCCGATGTAAGCACGCGCTACGAGCAGACCGCAGTGCTGGTCCCCGGTCGGACCGAATGGCGCCCTGCGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCACCGTCGTCGGccc                                                                                          >  4:93169/1‑149 (MQ=255)                                                       aCCAGCCTGCAGGAAGAAGCCGCCGGCGCCGATGTAAGCACGCGCTACGAGCAGACCGCAGTGCTGGTCCCCGGTCGGACCGAATGGCGCCCTGCGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCACAGTCCTCGGccc                                                                                          >  4:240493/1‑149 (MQ=255)                                                         cAGCCTGCAGGAAGAAGCCGCCGGCGCCGATGTAAGCACGCGCTACGAGCAGACCGCAGTGCTGGTCCCCGGTCGGACCGAATGGCGCCCTGCGCCATTGAAAAAACCACGAATCGAc                                                                                                                       >  3:102636/1‑118 (MQ=255)                                                         cAGCCTGCAGGAAGAAGCCGCCGGCGCCGATGTAAGCACGCGCTACGAGCAGACCGCAGTGCTGGTCCCCGGTCGGACCGAATGGCGCCCTGCGCCATTGAAAAAACCACGAATCGAc                                                                                                                       <  4:102636/118‑1 (MQ=255)                                                            ccTGCAGGAAGAAGCCGCCGGCGCCGATGTAAGCACGCGCTACGAGCAGACCGCAGTGCTGGTCCCCGGTCGGACCGAATGGCGCCCTGCGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCACCGTCGTCGGCCCGCCTg                                                                                     >  3:136666/1‑149 (MQ=255)                                                             cTGCAGGAAGAAGCCGCCGGCGCCGATGTAAGCACGCGCTACGAGCAGACCGCAGTGCTGGTCCCCGGTCGGACCGAATGGCGCCCTGCGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCACCGTCGTCGCACCGCCTgg                                                                                    >  4:25148/1‑149 (MQ=255)                                                               gCAGGAAGAAGCCGCCGGCGCCGATGTAAGCACGCGCTACGAGCAGACCGCAGTGCTGGTCCCCGGTCGGACCGAATGGCGCCCTGCGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCACCGGCGTCGGCCCGCCTGGCg                                                                                  >  7:295775/1‑149 (MQ=255)                                                                 aGGAAGAAGCCGCCGGCGCCGATGTAAGCACGCGCTACGAGCAGACCGCAGTGCTGGTCCCCGGTCGGACCGAATGGCGCCCTGTGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCACCGTCGTCGGCCCGCCTGGCGAg                                                                                >  4:106711/1‑149 (MQ=255)                                                                   gaagaaGCCGCCGGCGCCGATGTAAGCACGCGCTACGAGCAGACCGCAGTGCTGGTCCCCGGTCGGACCGAATGGCGCCCTGCGCCATTGAAAAAACCACGAATCGACg                                                                                                                      <  7:307300/109‑1 (MQ=255)                                                                   gaagaaGCCGCCGGCGCCGATGTAAGCACGCGCTACGAGCAGACCGCAGTGCTGGTCCCCGGTCGGACCGAATGGCGCCCTGCGCCATTGAAAAAACCACGAATCGACg                                                                                                                      >  8:307300/1‑109 (MQ=255)                                                                    aagaagCCGCCGGCGCCGATGTAAGCACGCGCTACGAGCAGACCGCAGTGCTGGTCCCCGGTCGGACCGAATGGCGCCCTGCGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCACCGTCGTCGGCCCGCCTGGCGAGGa                                                                              >  4:73621/1‑148 (MQ=255)                                                                     agaagCCGCCGGCGCCGATGTAAGCACGCGCTACGAGCAGACCGCAGTGCTGGTCCCCGGTCGGACCGAATGGCGCCCTGCGCCATTGAAAAAACCACGAAGCGACGGGCCGCACATGGCCACCGTCGTCGGCCCGCCTGGCGAGGaaa                                                                            <  1:263270/149‑1 (MQ=255)                                                                      gaagCCGCCGGCGCCGATGTAAGCACGCGCTACGAGCAGACCGCAGTGCTGGTCCCCGGTCGGACCGAATGGCGCCCTGCGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCACCGTCGTCGGCCCGCCTGGCGAGGAAAt                                                                           <  6:305338/149‑1 (MQ=255)                                                                       aagCCGCCGGCGCCGATGTAAGCACGCGCTACGAGCAGACCGCAGTGCTGGTCCCCGGTCGGACCGAATGGTGCCCTGCGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCACCGTCGTCGGCCCGCCTGGCGAGGAAATc                                                                          <  3:175733/149‑1 (MQ=255)                                                                         gccgccGGCGCCGATGTAAGCACGCGCTACGAGCAGACCGCAGTGCTGGTCCCCGGTCGGACCGAATGGCGCCCTGCGCCATTTAAAAAACCACGAATCGACGGGCCGCACATGGCCACCGTCGTCGGCCCGCCTGGCGAGGAAATCTa                                                                        >  6:298586/1‑149 (MQ=255)                                                                         gccgccGGCGCCGATGTAAGCACGCGCTACGAGCAGACCGCAGTGCTGGTCCCCGGTCGGACCGAATGGCGCCCTGCGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCACCGTCGTCGGCCCGCCTGGCGAGGAAATCTa                                                                        <  3:93169/149‑1 (MQ=255)                                                                           cgccgGCGCCGATGTAAGCACGCGCTACGAGCAGACCGCAGTGCTGGTCCCCGGTCGGACCGAATGGCGCCCTGCGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCACCGTCGTCGGCCCGCCTGGCGAGGAAATCTACt                                                                      <  7:155592/149‑1 (MQ=255)                                                                                    cGATGTAAGCACGCGCTACGAGCAGACCGCAGTGCTGGTCCCCGGTCGGACCGAATGGCGCCCTGCGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCACCGTCGTCGGCCCGCCTGGCGAGGAAATCTACTGCGATGAAt                                                             <  2:228307/149‑1 (MQ=255)                                                                                           aGCACGCGCTACAAGCAGACCGCAGTGCTGGTCCCCGGTCGGACCGAATGGCGCCCTGCGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCACCGTCGTCGGCCCGCCTGGCGAGGAAATCTACTGCGATGAATGGGGcc                                                       <  6:14197/148‑1 (MQ=255)                                                                                            gCACGCGCTACGAGCAGACCGCAGTGCTGGTCCCCGGTCGGACCGAATGGCGCCCTGCGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCACCGTCGTCGGCCCGCCTGGCGAGGAAATCTACTGCGATGAATGGGGCCgg                                                     <  6:161377/149‑1 (MQ=255)                                                                                                  gcTACGAGCAGACCGCAGTGCTGGTCCCCGGTCGGACCGAATGGCGCCCTGCGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCACCGTCGTCGGCCCGCCTGGCGAGGAAATCTACTGCGATGAATGGGGCCGGGTaaaa                                               >  2:146155/1‑149 (MQ=255)                                                                                                    tACGAGCAGACCGCAGTGCTGGTCCCCGGTCGGACCGAATGGCGCCCTGCGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCACCGTCGTCGGCCCGCCTGGCGAGGAAATCTACTGCGATGAAt                                                             >  4:20221/1‑133 (MQ=255)                                                                                                    tACGAGCAGACCGCAGTGCTGGTCCCCGGTCGGACCGAATGGCGCCCTGCGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCACCGTCGTCGGCCCGCCTGGCGAGGAAATCTACTGCGATGAAt                                                             <  3:20221/133‑1 (MQ=255)                                                                                                    tACGAGCAGACCGCAGTGCTGGTCCCCGGTCGGACCGAATGGCGCCCTGCGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCACCGTCGTCGGCCCGCCTGGCGAGGAAATCTACTGCGATGAATGGGGCCGGGTAAAAGt                                             >  6:196978/1‑149 (MQ=255)                                                                                                      cGAGCAGACCGCAGTGCTGGTCCCCGGTCGGACCGAATGGCGCCCTGCGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCACCGTCGTCGGCCCGCCTGGCGAGGAAATCTACTGCGATGAATGGGGCCGGGTAAAAGTCa                                           <  8:108532/149‑1 (MQ=255)                                                                                                         gCAGACCGCAGTGCTGGTCCCCGGTCGGACCGAATGGCGCCCTGCGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCACCGTCGTCGGCCCGCCTGGCGAGGAAATCTACTGCGATGAATGGGGCCGGGTAAAAGTCAGtt                                        >  1:176833/1‑149 (MQ=255)                                                                                                            gACCGCAGTGCTGGTCCCCGGTCGGACCGAATGGCGCCCTGCGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCACCGTCGTCGGCCCGCCTGGCGAGGAAATCTACTGCGATGAATGGGGCCGGGTAAAAGTCAGTTTcc                                     >  3:189805/1‑149 (MQ=255)                                                                                                            gACCGCAGTGCTGGTCCCCGGTCGGACCGAATGGCGCCCTGCGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCACCGTCGTCGGCCCGCCTGGCGAGGAAATCTACTGCGATGAATGGGGCCGGGTAAAAGTCAGTTTcc                                     >  1:226860/1‑149 (MQ=255)                                                                                                              ccGCAGTGCTGGTCCCCGGTCGGACCGAATGGCGCCCTGCGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCACCGTCGTCGGCCCGCCTGGCGAGGAAATCTACTGCGATGAATGGGGCCGGGTAAAAGTCAGTTTcccc                                   <  4:189805/149‑1 (MQ=255)                                                                                                               cGCAGTGCTGGTCCCCGGTCGGACCGAATGGCGCCCTGCGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCACCGTCGTCGGCCCGCCTGGCGAGGAAATCTACTGCGATGAATGGGGCCGGGTAAAAGTCAGTTTCCCCt                                  >  5:138843/1‑149 (MQ=255)                                                                                                               cGCAGTGCTGGTCCCCGGGCGGACCGAATGGCGCCCTGCGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCACCGACGTCGGCCCGCCTGGCGAGGAAATCTACTGCGATGAATTGGGCCGGGTAAAAGTCAGTTTCCCCt                                  <  6:171450/149‑1 (MQ=255)                                                                                                                 cAGTGCTGGTCCCCGGTCGGACCGAATGGCGCCCTGCGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCACCGTCGTCGGCCCGCCTGGCGAGGAAATCTACTGCGATGAATGGGGCCGGGTAAAAGTCAGTTTCCCCTgg                                <  3:73621/149‑1 (MQ=255)                                                                                                                   gTGCTGGTCCCCGGTCGGACCGTATGGCGCCCTGCGCCATTGAAAAAACCACGAATCGACGGTCCGCACATGGCCAGCGTCGTCGGCCCGCCTGGCGATGAAATCTACTGCGATGAATGGGGCCGGGTAAAAGTCAGTTTCCCCTGGGa                              <  3:240493/149‑1 (MQ=255)                                                                                                                    tGCTGGTCCCCGGTCGGACCGAATGGCGCCCTGCGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCACCGTCGTCGGCCCGCCTGGCGAGGAAATCTACTGCGATGAATGGGGCCGGGTAAAAGTCAGTTTCCCCTGGGAc                             >  7:26001/1‑149 (MQ=255)                                                                                                                        ggTCCCCGGTCGGACCGAATGGCGCCCTGCGCCATTGAAAAAACCACGAATAGACGGGCCGCACATGGCCACCGTCGTCGGCCCGCCTGGCGAGGAAATCTAGTGCGATGAATGGGGCCGGGTAAAAGTCAGTTTCCCCTGGGACcgc                          >  1:5531/1‑148 (MQ=255)                                                                                                                            cccGGTCGGACCGAATGGCGCCCTGCGCCATTTAAAAAAACACGAATCGACGGGCCGCACATGGCCACCCTCGTCGGCCCGCCAGGCGAGGAAAACAACAGCGATGAATGGGGCCGGGTAAAAGTCAATTTACCAGGGGCCCGCGAAAg                     >  2:265521/1‑149 (MQ=18)                                                                                                                            cccGGTCGGACCGAATGGCGCCCTGCGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCACCGTCGTCGGCCCGCCTGGCGAGGAAATCTACTGCGATGAATGGGGCCGGGTAAAAGTCAGTTTCCCCTGGGACCGCGaaa                      <  6:138843/148‑1 (MQ=255)                                                                                                                             ccGGTCGGACCGAATGGCGCCCTGTGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCACCGTCGTCGGCCCGCCTGGCGAGGAAATCTACTGCGATGAATGGGGCCGTGTAAAAGTCAGTTTCCCCTGGGACCGCGAAAGc                    <  3:106711/149‑1 (MQ=255)                                                                                                                               ggTCGGACCGAATGGCGCCCTGCGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCACCGTCGTCGGCCCGCCTGGCGAGGAAATCTACTGCGATGAATGGGGCCGGGTAAAAGTCAGTTTCCCCTGGGACCGCGAAAGCCa                  <  8:26001/149‑1 (MQ=255)                                                                                                                                    gACCGAATGGCGCCCTGCGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCACCGTCGTCGGCCCGCCTGGCGAGGAAATCTACTGCGATGAATGGGGCCGGGTAAAAGTCAGTTTCCCCTGGGACCGCGAAAGCCAGaac              >  2:331010/1‑148 (MQ=255)                                                                                                                                    gACCGAATGCCGCCCTGCGCAATTGAAAAAACCACGAATCGACGGGCCGCAAATGGCCACCGTCGTCGGCCCCCCTGGCGAGTAAATCTACACGGATGAAAGGGAACGGGTAAAAGTCAGTTACACATGGGACCCCCAAAGCCAGaac              >  6:215251/1‑148 (MQ=18)                                                                                                                                     aCCGAATGGCGCCCTGCGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCACCGTCGTCGGCCCGCCTGGCGAGGAAATCTACTGCGATGAATGGGGCCGGGTAAAAGTCAGTTTCCCCTGGGACCGCGAAAGCCAGagcaa            >  3:264686/1‑149 (MQ=255)                                                                                                                                     aCCGAATGGCGCCCTGCGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCACCGTCGTCGGCCCGCCTGGCGAGGAAATCTACTGCGATGAATGGGGCCGGGTAAAAGTCAGTTTCCCCTGGGACCGCGAAAGCCAGaacac            >  5:250254/1‑148 (MQ=255)                                                                                                                                     aCCGAATGGCGCCCTGCGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCACCGTCGTCGGCCCGCCTGGCGAGGAAATCTACTGCGATGAATGGGGCCGGGTAAAAGTCAGTTTCCCCTGGGACCGCGAAAGCCAGaacaa            >  1:262576/1‑149 (MQ=255)                                                                                                                                        gAATGGCGCCCTGCGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCACCGTCGTCGGCCCGCCTGGCGAGGAAATCTACTGCGATGAATGGGGCCGGGTAAAAGTCAGTTTCCCCTGGGACCGCGAAAGCCAGAACAACGa         <  5:196978/149‑1 (MQ=255)                                                                                                                                         aaTGGCGCCCTGCGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCACCGTCGTCGGCCCGCCTGGCGAGGAAATCTACTGCGATGAATGGGGCCGGGTAAAAGTCAGTTTCCCCTGGGACCGCGAAAGCCAGAACAACGag        <  4:136666/149‑1 (MQ=255)                                                                                                                                         aaTGGCGCCCTGCGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCACCGTCGTCGGCCCGCCTGGCGAGGAAATCTACTGCGATGAATGGGGCCGGGTAAAAGTCAGTTTCCCCTGGGACCGCGAAAGCCAGAACAACGag        >  6:231965/1‑149 (MQ=255)                                                                                                                                         aaTGGCGCAGGGCGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCACCGTCGTCGGCCCGCCTGGCGAGGAAATCTACTGCGATGAATGGGGCCGGGTAAAAGTCAGTTTCCCCTGGGACCGCGAAAGCCAGAACAACGag        >  6:165528/1‑149 (MQ=255)                                                                                                                                              cgcCCTGCGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCACCGTCGTCGGCCCGCCTGGCGAGGAAATCTACTGCGATGAATGGGGCCGGGTAAAAGTCAGTTTCCCCTGGGACCGCGAAAGCCAGAACAACGagttcag   <  1:146155/149‑1 (MQ=255)                                                                                                                                               gcCCTGCGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCACCGTCGTCGGCCCGCCTGGCGAGGAAATCTACTGCGATGAATGGGGCCGGGTAAAAGTCAGTTTCCCCTGGGACCGCGAAAGCCAGAACAACGagttcagt  <  2:176833/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                  GACCTCAATGCGCATTGGCGGGTCAACACGGTTACCCATAAAGGCCACCAGTTCACCAGCCTGCAGGAAGAAGCCGCCGGCGCCGATGTAAGCACGCGCTACGAGCAGACCGCAGTGCTGGTCCCCGGTCGGACCGAATGGCGCCCTGCGCCATTGAAAAAACCACGAATCGACGGGCCGCACATGGCCACCGTCGTCGGCCCGCCTGGCGAGGAAATCTACTGCGATGAATGGGGCCGGGTAAAAGTCAGTTTCCCCTGGGACCGCGAAAGCCAGAACAACGAGTTCAGT  >  NC_002947/4612108‑4612398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑