Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,613,456	G→A	8.3%	A104A (GCG→GCA)	PP_4084 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,613,456	0	G	A	8.3%	164.2 / 4.6	61	A104A (GCG→GCA)	PP_4084	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (27/28);  new base A (2/3);  total (30/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

AACTGAACGTATTACGGAAGAAGTCAAAGGCGCTGACCATTACGCCCGCGAAAGTGACCCGCCTTGGAAGCAATGAGCATCCCGTCGACAGTCGGGAAATGGCCATCCAGTTCATGGTCGGCGACAAGCCCCACTACCAGCTTCAGGCGGCCTGCCTGATACCGGGACAGCAACGGATGCTGGTGCTCAACTACAGCAAGCCTGGGCCTTTGAGTGACGACGATATCAGCCACTGGCGCGCGCTTAAAGACGAGCTCCGATTTGCCTGAGCCTCTCCACGCCCCAGGAC  >  NC_002947/4613308‑4613596                                                                                                                                                     |                                                                                                                                             aaCTGAACGTATTACGGAAGAAGTCAAAGGCGCTGACCATTACGCCCGCGAAAGTGACCCGCCTTGGAAGCAATGAGCATCCCGTCGACAGTCGGGAAATGGCCATCCAGTTCATGGTCGGCGACAAGCCCCACTACCAGCTTCAggcg                                                                                                                                              <  1:87662/149‑1 (MQ=255)         gTATTACGGAAGAAGTCAAAGGCGCTGACCATTACGCCCGCGAAAGTGACCCGCCTTGGAAGCAATGAGCATCCCGTCGACAGTCGGGAAATGGCCATCCAGTTCATGGTCGGCGACAAGCCCCACTACCAGCTTCAGGCGgcctgcct                                                                                                                                      <  2:169211/149‑1 (MQ=255)            ttACGGAAGAAGTCAAAGGCGCTGACCATTACGCCCGCGAAAGTGACCCGCCTTGGAAGCAATGAGCATCCCGTCGACAGTCGGGAAATGGCCATCCAGTTCATGGTCGGCGACAAGCCCCACTACCAGCTTCAGGCGGCCTGCCTGa                                                                                                                                    >  1:35872/1‑148 (MQ=255)               cGGAAGAAGTCAAAGGCGCTGACCATTACGCCCGCGAAAGTGACCCGCCTTGGAAGCAATGAGCATCCCGTCGACAGTCGGGAAATGGCCATCCAGTTCATGGTCGGCGACAAGCCCCACTACCAGCTTCAGGCAGGCCGCCTGaagct                                                                                                                                <  7:50588/149‑5 (MQ=255)               cGGAAGAAGACAAAGGCGCTGACCATTACCCCCGCGAAAGAGACCCCCCTTGGAAGCAATGAGCATCACGTCGACAGACGGGAAAAGGCCATCCAGATCAAGGTCGGCGACAACCCCCACTACCAGCTTCAGGAAGGCCGCCTGaa                                                                                                                                   >  8:163955/1‑145 (MQ=255)               aggAAGAAGTGAAAGGCGCTGACCATTACGCCCGCGAAAGTGACCCTCGTTGGAACCAATGAGCTTCCCGTCGTCAGTAGGGAAATGGCCATCCAGTTCATGGTCGGCGACAAGCCCCACTACCAGCTTCAGGCAGGCCGCCTGaa                                                                                                                                   <  7:163955/145‑2 (MQ=255)                   tgaagTCAAAGGGGCTGACCATTACGCCGGCTTTAGTGACCCGCCTTGGAAGCAATGAGCATCCCGTCGACAGTCGTGAAATGGCCATCCTTTTCATTGTCGGCGACAAGCCCGACTACCAGCTTCTGGCGTCCTGCCTGATAcctgta                                                                                                                            <  4:242529/148‑5 (MQ=255)                       gTCAAAGGCGCTGACCATTACGCCCGCGAAAGTGACCCGCCTTGGAAGCAATGAGCATCCCGTCGACAGTCGGGAAATGGCCATCCAGTTCACGGTCGGCGACAAGCCCCACTACCAGCTTCAGGCGGCCTGCCTGATACCGGGACAGc                                                                                                                        <  4:175740/149‑1 (MQ=255)                          aaaGGCGCTGACCATTACGCCCGCGAAAGTGACCCGCCTTGGAAGCAATGAGCATCCCGTCGACAGTCGGGAAATGGCCATCCAGTTCATGGTCGGCGACAAGCCCCACTACCAGCTTCAGGCGGCCTGCCTGATACCGGGACAGCaa                                                                                                                      >  8:293778/1‑148 (MQ=255)                          aaaGGCGCTGACCATTACGCCCGCGAAAGTGACCCGCCTTGGAAGCAATGAGCATCCCGTCGACAGTCGGGAAATGGCCATCCAGTTCATGGTCGGCGACAAGCCCCACAACCAGCTTCAGGCGGCCTGCCTGATACCGGGACAGCAAc                                                                                                                     >  8:311672/1‑149 (MQ=255)                                     ccATTACGCCCGCGAAAGTGACCCGCCTTGGAAGCAATGAGCATCCCGTCGACAGTCGGGAAATGGCCATCCAGTTCATGGTCGGCGACAAGCCCCACTACCAGCTTCAGGCGGCCTGCCTGATACCGGGACa                                                                                                                          >  4:135528/1‑133 (MQ=255)                                     ccATTACGCCCGCGAAAGTGACCCGCCTTGGAAGCAATGAGCATCCCGTCGACAGTCGGGAAATGGCCATCCAGTTCATGGTCGGCGACAAGCCCCACTACCAGCTTCAGGCGGCCTGCCTGATACCGGGACa                                                                                                                          <  3:135528/133‑1 (MQ=255)                                      cATTACGCCCGCGAAAGTGACCCGCCTTGGAAGCAATGAGCATCCCGTCGACAGTCGGGAAATGGCCATCCAGTTCATGGTCGGCGACAAGCCCCACTACCAGCTTCAGGCGGCCTGCCTGATACCGGGACAGCAACGGATGCTGGTGc                                                                                                         >  5:229055/1‑149 (MQ=255)                                        ttACGCCCGCGAAAGTGACCCGCCTTGGAAGCAATGAGCATCCCGTCGACAGTCGGGAAATGGCCATCCAGTTCATGGTCGGCGACAAGCCCCACTACCAGCTTCAGGCGGCCTGCCTGATACCGGGACAGCAACGGATGCTGGTg                                                                                                          <  3:348400/146‑1 (MQ=255)                                        ttACGCCCGCGAAAGTGACCCGCCTTGGAAGCAATGAGCATCCCGTCGACAGTCGGGAAATGGCCATCCAGTTCATGGTCGGCGACAAGCCCCACTACCAGCTTCAGGCGGCCTGCCTGATACCGGGACAGCAACGGATGCTGGTg                                                                                                          >  4:348400/1‑146 (MQ=255)                                        ttACGCCCGCGAAAGTGACCCGCCTTGGAAGCAATGAGCATCCCGTCGACAGTCGGGAAATGGCCATCCAGTTCATGGTCGGCGACAAGCCCCACTACCAGCTTCAGGCGGCCTGCCTGATACCGGGACAGCAACGGATGCTGGTGCt                                                                                                        >  1:287491/1‑148 (MQ=255)                                           cGCCCGCGAAAGTGACCCGCCTTGGAAGCAATGAGCATCCCGTCGACAGTCGGGAAATGGCCATCCAGTTCATGGTCGGCGACAAGCCCCACTACCAGCTTCAGGCGGCCTGCCTGATACCGGGACAGCAACGGATGCTGGTGCTCAAc                                                                                                    >  1:185446/1‑149 (MQ=255)                                            gCCCGCGAAAGTGACCCGCCTTGGAAGCAATGAGCATCCCGTCGACAGTCGGGAAATGGCCATCCAGTTCATGGTCGGCGACAAGCCCCACTACCAGCTTCAGGCGGCCTGCCAGATACCGGGACAGCAACGGATGCTGGTGCTCAAc                                                                                                    >  2:280493/1‑148 (MQ=255)                                                gcgAAAGTGACCCGCCTTGGAAGCAATGAGCATCCCGTCGACAGTCGGGAAATGGCCATCCAGTTCATGGTCGGCGACAAGCCCCACTACCAGCTTCAGGCGGCCTGCCTGATACCGGGACAGCAACGGATGCTGGTGCTCAACTACAg                                                                                               >  5:362045/1‑149 (MQ=255)                                                  gAAAGTGACCCGCCTTGGAAGCAATGAGCATCCCGTCGACAGTCGGGAAATGGCCATCCAGTTCATGGTCGGCGACAAGCCCCACTACCAGCTTCAGGCGGCCTGCCTGATACCGGGACAGCAACGGATGCTGGTGCTCAACTACAGc                                                                                              >  1:339953/1‑148 (MQ=255)                                                          cccGCCTTGGAAGCAATGAGCATCCCGTCGACAGTCGGGAAATGGCCATCCAGTTCATGGTCGGCGACAAGCCCCACTACCAGCTTCAGGCGGCCTGCCTGATACCGGGACAGCAACGGATGCTGGTGCTCAACTACAGCAAGCCTggg                                                                                     <  6:229055/149‑1 (MQ=255)                                                           ccGCCTTGGAAGCAATGAGCATCCCTTCGACAGTCGGGAAATGGCCATCCAGTTAATGGTCGGCGACAAGCCCCACTACCAGCTTCAGGCGGCCTCGCTGATaac                                                                                                                                >  6:281247/1‑103 (MQ=255)                                                           ccGCCTTGGAAGCAATGAGCATCCCGTCGACAGTCGGGAAATGGCCATCCAGTTCATGGTCGGCGACAAGCCCCACTACCAGCTTCAGGCGGCCTGCCTGATACCGGGACAGCAACGGATGCTgg                                                                                                            <  8:278231/125‑1 (MQ=255)                                                           ccGCCTTGGAAGCAATGAGCATCCCGTCGACAGTCGGGAAATGGCCATCCAGTTCATGGTCGGCGACAAGCCCCACTACCAGCTTCAGGCGGCCTGCCTGATACCGGGACAGCAACGGATGCTgg                                                                                                            >  7:278231/1‑125 (MQ=255)                                                                ttGGAAGCAATGAGCATCCCGTCGACAGTCGGGAAATGGCCATCCAGTTCATGGTCGGCGACAAGCCCCACTACCAGCTTCAGGCGGCCTGCCTGATACCGGGACAGCAACGGATGCTGGTGCTCAACTACAGCAAGCCTGGGCCttt                                                                                >  3:304491/1‑148 (MQ=255)                                                                  ggAAGCAATGAGCATCCCGTCGACAGTCGGGAAATGGCCATCCAGTTCATGGTCGGCGACAAGCCCCACTACCAGCTTCAGGCGGCCTGCCTGATACCGGGACAGCAACGGATGCTGGTGCTCAACTACAGCAAGCCTGGGCCTTTGa                                                                              <  4:304491/148‑1 (MQ=255)                                                                  ggAAGCAATGAGCATCCCGTCGACAGTCGGGAAATGGCCATCCAGTTCATGGTCGGCGACAAGCCCCACTACCAGCTTCAGGCGGCCTGCCTGATACCGGGACAGCAACGGATGCTGGTGCTCAACTACAGCAAGCCTGGGCCTTTGa                                                                              <  7:311672/148‑1 (MQ=255)                                                                  ggAAGCAATGAGCATCCCGTCGACAGTCGGGAAATGGCCATCCAGTTCATGGTCGGCGACAAGCCCCACTACCAGCTTCAGGCGGCCTGCCTGATACCGGGACAGCAACGGATGCTGGTGCTCAACTACAGCAAGCCTGGGCCTTTGa                                                                              <  2:339953/148‑1 (MQ=255)                                                                  ggAAGCAATGAGCATCCCGTCGACAGTCGGGAAATGGCCATCCAGTTCATGGTCGGCGACAAGCCCCACTACCAGCTTCAGGCGGCCTGCCAGATACCGGGACAGCAACGGATGCTGGTGCTCAACTACAGCAAGCCTGGGCCTTTGa                                                                              <  1:280493/148‑1 (MQ=255)                                                                         aTGAGCATCCCGTCGACAGTCGGGAAATGGCCATCCAGTTCATGGTCGGCGACAAGCCCCACTACCAGCTTCAGGCGGCCTGCCTGATACCGGGACAGCAACGGATGCTGGTGCTCAACTACAGCAAGCCTGGGCCTTTGAGTgacgac                                                                      <  7:293778/149‑1 (MQ=255)                                                                             gCATCCCGTCGACAGTCGGGAAATGGCCATCCAGTTCATGGTCGGCGACAAGCCACACTACCAGCTTCAGGCCGCCTGCCTGATACCGGGACAGCAACGGATGCTGGTGCTCAACTACAGCAAGCCTGGGCCTTTGAGTGACGACGata                                                                  >  2:70601/1‑149 (MQ=255)                                                                                tCCCGTCGACAGTCGGGAAATGGCCATCCAGTTCATGGTCGGCGACAAGCCCCTCTACCAGCTTCAGGCGGCCTGCCTGATACCGGGACAGCAACGGATGCTGGTGCTCAACTACAGCAAGCCTGGGCCTTTGAGTGACGACGATATCa                                                               <  2:287491/149‑1 (MQ=255)                                                                                tCCCGTCGACAGTCGGGAAATGGCCATCCAGTTCATGGTCGGCGACAAGCCCCACTACCAGCTTCAGGCGGCCTGCCTGATACCGGGACAGCAACGGATGCTgg                                                                                                            <  2:29308/104‑1 (MQ=255)                                                                                tCCCGTCGACAGTCGGGAAATGGCCATCCAGTTCATGGTCGGCGACAAGCCCCACTACCAGCTTCAGGCGGCCTGCCTGATACCGGGACAGCAACGGATGCTgg                                                                                                            >  1:29308/1‑104 (MQ=255)                                                                                 cccGTCGACAGTCGGGAAATGGCCATCCAGTTCATGGTCGGCGACGAGCCCCACTACCAGCTTCAGGCGGCCTGCCTGATACCGGGACAGCAACGGATGCTGGTGCTCACCTACAGCAAGCCTGGGCCTTTGAGTGCCGACGCTATCAg                                                              >  4:181187/1‑149 (MQ=255)                                                                                 cccGTCGACAGTCGGGAAATGGCCATCCAGTTCATGGTCGGCGACGAGCCCCACTACCAGCTTCAGGCGGCCTGCCTGATACCGGGACAGCAACGGATGCTGGTGCTCAACTACAGCAAGCCTGGGCCTTTGAGTCACGCCGATATCAg                                                              >  4:175622/1‑149 (MQ=255)                                                                                     tCGACAGTCGGGAAATGGCCATCCAGTTCATGGTCGGCGACAAGCCCCACTACCAGCTTCAGGCGGCCTGCCTGATACCGGGACAGCAACGGATGCTGGTGCTCAACTACAGCAAGCCTGGGCCTTTGAGTGACGACGata                                                                  <  2:227785/141‑1 (MQ=255)                                                                                     tCGACAGTCGGGAAATGGCCATCCAGTTCATGGTCGGCGACAAGCCCCACTACCAGCTTCAGGCGGCCTGCCTGATACCGGGACAGCAACGGATGCTGGTGCTCAACTACAGCAAGCCTGGGCCTTTGAGTGACGACGata                                                                  >  1:227785/1‑141 (MQ=255)                                                                                         cAGTCGGGAAATGGCCATCCAGTTCATGGTCGGCGACAAGCCCCACTACCAGCTTCAGGCGGCCTGCCTGATACCGGGACAGCAACGGATGCTGGTGCTCAACTACAGCAAGCCTGGGCCTTTGAGTGACGACGATATCAGCCACTGgc                                                      <  2:185446/149‑1 (MQ=255)                                                                                          aGTCGGGAAATGGCCATCCAGTTCATGGTCGGCGACAAGCCCCACTACCAGCTTCAGGCGGCCTGCCTGATACCGGGACAGCAACGGATGCTGGTGCTCAACTACAGCAAGCCTGGGCCTTTGAGTGACGACGATATCAGCCACTGgcg                                                     >  2:178215/1‑149 (MQ=255)                                                                                            tCGGGAAATGGCCATCCAGTTCATGGTCGGCGACAAGCCCCACTACCAGCTTCAGGCGGCCTGCCTGATACCGGGACAGCAACGGATGCTGGTGCTCAACTACAGCAAGCCTgg                                                                                      >  1:221969/1‑114 (MQ=255)                                                                                            tCGGGAAATGGCCATCCAGTTCATGGTCGGCGACAAGCCCCACTACCAGCTTCAGGCGGCCTGCCTGATACCGGGACAGCAACGGATGCTGGTGCTCAACTACAGCAAGCCTgg                                                                                      <  2:221969/114‑1 (MQ=255)                                                                                               ggAAATGGCCATCCAGTTCATGGTCGGCGACGAGCCCCCCTTCCAGCTTCAGGCGGCCTGCCTGATACCGGGCCAGCAACGGATGGTGGTGCTGAACTACAGCAAGCCTGGGCCGTTGAGTGACGACGATATCAGCCACTGgcgcgcgc                                                <  3:181187/149‑1 (MQ=255)                                                                                               ggAAATGGCCATCCAGTTCATGGTCGGCGACGAGCCCCACTACCAGCTTCAGGCGGCCTGCCTGATACCGGGACAGCAACGGATGCTGGTGCTCAACTACAGCAATCCTGGGCCTTTGAGTGACGACGATATCAGCCACTGgcgcgcgc                                                <  3:175622/149‑1 (MQ=255)                                                                                                      gCCATCCAGTTCATTGTCGGCGACAAGCCCCACTACCAGCTTCAGGCGGCCTGCCTGATACCGGGACAGCAACGGATGCTGGTGCTCAACTACAGCAAGCCTGGGCCTTTGAGTGACGACGATATCAGCCACTGGCGCGCGCTTAAAg                                          <  3:10537/148‑1 (MQ=255)                                                                                                      gCCATCCAGTTCATGGTCGGCGACAAGCCCCACTACCAGCTTCAGGCGGCCTGCCTGATACCGGGACAGCAACGGATGCTGGTGCTCAACTACAGCAAGCCTGGGCCTTTGAGTGACGACGATATCAGCCACTGGCGCGCGCTTAAAg                                          <  1:70601/148‑1 (MQ=255)                                                                                                                    ggTCGGCGACAAGCCCCACTACCAGCTTCAGGCGGCCTGCCTGATACCGGGACAGCAACGGATGCTGGTGCTCAACTACAGCAAGCCTGGGCCTTTGAGTGCCGACGATATCAGCCACTGGCGCGCGCTTAAAGACGAGCTCCGATTTg                           >  1:313954/1‑149 (MQ=255)                                                                                                                    ggTCGGCGACAAGCCCCACTACCAGCTTCAGGCGGCCTGCCTGATACCGGGACAGCAACGGATGCTGGTGCTCAACTACAGCAAGCCTGGGCCTTTGAGTGACGACGATATCAGCCACTGGCGCGCGCTTAAAGACGAGCTCCGATTTg                           >  6:184808/1‑149 (MQ=255)                                                                                                                     gTCGGCGACAAGCCCCACTACCAGCTTCAGGCGGCCTGCCTGATACCGGGACAGCAACGGATGCTGGTGCTCAACTACAGCAAGCCTGGGCCTTTGAGTGACGACGATATCAGCCACTGGCGCGCGCTTAAAGACGAGCTCCGATTTGc                          <  2:35872/149‑1 (MQ=255)                                                                                                                          cGACAAGCCCCACTACCAGCTTCAGGCGGCCTGCCTGATACCGGGACAGCAACGGATGCTGGTGCTCAACTACAGCAAGCCTGGGCCTTTGAGTGACGACGATATCAGCCACTGGCGCGCGCTTAAAGACGAGCTCCCATTTGCCTGAg                     >  8:160570/1‑149 (MQ=255)                                                                                                                           gACAAGCCCCACTACCAGCTTCAGGCGGCCTGCCTGATACCGGGACAGCAACGGATGCTGGTGCTCAACTACAGCAAGCCTGGGCCTTTGAGTGACGACGATATCAGCCACTGGCGCGCGCTTAAAGACGAGCTCCGATTTGCCTGAGc                    <  4:5086/149‑1 (MQ=255)                                                                                                                                     aCTACCAGCTTCAGGCGGCCTGCCTGATACCGGGACAGCAACGGATGCTGGTGCTCAACTACAGCAAGCCTGGGCCTTTGAGTGACGACGATATCAGCCACTGGCGCGCGCTTAAAGACGAGCTCCGATTTGCCTGAGCCTCTCCACGc          <  6:362045/149‑1 (MQ=255)                                                                                                                                       ttccAGCTTCCGGCGGCCTGCCTGATAACGGCACAGCAACGTATGCTTGTGCTCAACTAAAGCAAGCCTGGGCCTTTGAGTGACGACGAAATCAGCCACTTGCGCGCGCTTAAAGACGAGCTACGATTTGCCTTAGc                    <  4:296900/135‑1 (MQ=255)                                                                                                                                       tACCATCTTCAGGCGGCTTGCCTGATACCGGGACAGCAACGGTTGCTGGTGCTCATCTACAGCAAGCCTGGGCCTTTGAGTGACGACGATATCAGCCACTGGCGCGCGCTTAAAGACGAGCTCCGATTTTCCTGAGCCTCTCCACGccc        <  2:313954/149‑1 (MQ=255)                                                                                                                                       tACCAGCTTCAGGCGGCCTGCCTGATACCGGGACAGCAACGGATGCTGGTGCTCAACTACAGCAAGCCTGGGCCTTTGAGTGACGACGATATCAGCCACTGGCGCGCGCTTAAAGACGAGCTCCGATTTGCCTGAGc                    >  3:296900/1‑137 (MQ=255)                                                                                                                                         ccAGCTTCAGGCGGCCTGCCTGATACCGGGACAGCAACGGATGCTGGTGCTCAACTACAGCAAGCCTGGGCCTTTGAGTGACGACGATATCAGCCACTGGCGCGCGCTTAAAGACGAGCTCCGATTTGCCTGAGCCTCTCCACGCCCCa      <  5:184808/149‑1 (MQ=255)                                                                                                                                          cggtatCAGGCAGGCCGCCTGATACCGGGACAGCAACGGTTGCTGGTGCTCAACTACAGCAAGCCTGGGCCTTTGAGTGACGACGATATCAGCCACTGGCGCGCGCTTAAAGACGAGCTCCGATTTGCCTGAGc                    <  6:137003/129‑1 (MQ=255)                                                                                                                                          cggtatCAGGCAGGCCGCCTGATACCGGGACAGCAACGGATGCTGGTGCTCAACTACAGCAAGCCTGGGCCTTTGAGTGACGACGATATCAGCCACTGGCGCGCGCTTAAAGACGAGCTCCGCTTTGCCTGAGc                    >  5:137003/6‑134 (MQ=255)                                                                                                                                          cAGCTTCAGGCGGCCTGCCTGATACCGGGACAGCAACGGATGCTGGTGCTCAACTACAGCAAGCCTGGGCCTTTGAGTGACGACGATATCAGCCACTGGCGCGCGCTTAAAGACGAGCTCCGATTTGCCTGAGCCTCTCCACGCCCCAg     <  1:178215/149‑1 (MQ=255)                                                                                                                                            gCTTCAGGCGGCCTGCCTGATACCGGGACAGCAACGGATGCTGGTGCTCAACTACAGCAAGCCTGGGCCTTTGAGTGACGACGATATCAGCCACTGGCGCGCGCTTAAAGACGAGCTCCGATTTGCCTGAGCCTCTCCACGCCCCAGGa   >  5:115134/1‑149 (MQ=255)                                                                                                                                             cTTCAGGCGGCCTGCCTGATACCGGGACAGCAACGGATGCTGGTGCTCAACTACAGCAAGCCTGGGCCTTTGAGTGACGACGATATCAGCCACTGGCGCGCGCTTAAAGACGAGCTCCGATTTGCCTGAGCCTCTCCACGCCCCAGGa   >  2:37168/1‑148 (MQ=255)                                                                                                                                              ttCAGGCGGCCTGCCTGATACCGGGACAGCAACGGATGCTGGTGCTCAACTACAGCAAGCCTGGGCCTTTGAGTGACGACGATATCAGCCACTGGCGCGCGCTTAAAGACGAGCTCCGATTTGCCTGAGCCTCTCCACGCCCCAGGAc  >  5:106002/1‑148 (MQ=255)                                                                                                                                                     |                                                                                                                                             AACTGAACGTATTACGGAAGAAGTCAAAGGCGCTGACCATTACGCCCGCGAAAGTGACCCGCCTTGGAAGCAATGAGCATCCCGTCGACAGTCGGGAAATGGCCATCCAGTTCATGGTCGGCGACAAGCCCCACTACCAGCTTCAGGCGGCCTGCCTGATACCGGGACAGCAACGGATGCTGGTGCTCAACTACAGCAAGCCTGGGCCTTTGAGTGACGACGATATCAGCCACTGGCGCGCGCTTAAAGACGAGCTCCGATTTGCCTGAGCCTCTCCACGCCCCAGGAC  >  NC_002947/4613308‑4613596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑