| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 353,067 | A→G | 100% | intergenic (+34/+84) | hisF → / ← cbcV | imidazole glycerol phosphate synthase subunit HisF/choline/betaine/carnitine ABC transporter ATP binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 353,067 | 0 | A | G | 100.0% | 87.6 / NA | 27 | intergenic (+34/+84) | hisF/cbcV | imidazole glycerol phosphate synthase subunit HisF/choline/betaine/carnitine ABC transporter ATP binding protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (13/14); total (13/14) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCAGGCACGCCCGCTCCCACAGG‑TTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAA‑CCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATCCCCACCA > NC_002947/352924‑353212 | tGGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCAc > 5:316406/1‑149 (MQ=255) cTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACCCCCGCACCCCCCGG‑ttttggga > 4:5736/1‑144 (MQ=255) cAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGc < 6:167783/120‑1 (MQ=255) cAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGc > 5:167783/1‑120 (MQ=255) ttCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGG‑TTTGCTGAGATCTCtgt > 8:182202/1‑143 (MQ=255) ttCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGG‑TTTGCTGAGATCTCtgt < 7:182202/143‑1 (MQ=255) aCTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGG‑TTTGCTGAGATCTCTGTGGGAGCGGGc < 4:96319/149‑1 (MQ=255) cTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGCTTTGCTGAGATCTCTGTGGGAGCGGGc < 1:199121/149‑1 (MQ=255) cTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGG‑TTTGCTGAGATCTCTGTGGGAGCGGGCg < 4:84529/149‑1 (MQ=255) tttGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGAACCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGG‑t > 5:276317/1‑121 (MQ=255) tttGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGAACCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGG‑t < 6:276317/121‑1 (MQ=255) cGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGG‑TTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCcg > 2:78610/1‑149 (MQ=255) aGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGG‑TTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGcc > 7:271468/1‑149 (MQ=255) ggAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCGCCCACACG‑GTTGCAGTGATCTCTGTGGGCGCGGGCGGGCCCGCGCAGGGCCGCGGAg > 1:337179/1‑149 (MQ=37) cAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGG‑TTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGccc > 3:233596/1‑149 (MQ=255) cAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGG‑TTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGccc > 6:146387/1‑149 (MQ=255) gCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCCCAGG‑GTTGCTGAGATGTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGCAGCGGCCCCAAt > 1:147905/1‑149 (MQ=38) gCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGG‑TTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCaa < 6:316406/148‑1 (MQ=255) gtaTCGGCGTTCGCTGATCATTTGCCCGGGGCGGTTTTCCTGCCCTTCGCGGGCACGCCCGATCCCAAAGG‑TTTGCTGAGATCTCTGGGGGTGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAA‑CCGTGGTTCtt < 2:337179/147‑1 (MQ=37) atggtcgtTCTCTTATCCATTGACCGGGGCTGCTTTGCTGCCCTTCGCGGGCACGCCCGCTCCCACCGG‑TTGGCTGATATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAA‑CCGTGGTTCTTGc < 4:43502/146‑1 (MQ=38) aTCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCCCAGG‑GTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGc > 8:105543/1‑119 (MQ=255) aTCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGG‑TTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGCAGGGCCGCGAAGCGGCCCCAATTGCTTCAA‑CCGGGGTTCTTGCCGCGCAGCGCGTgg > 5:169448/1‑149 (MQ=255) aTCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGG‑TTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAA‑CCGTGGTTCTTGCCGAGCAGCGCGTg < 5:146387/148‑1 (MQ=255) tCCTTTGCCGGGGGCTGCTTTGACGCCCTTCGCGGGCACGCCCGCGCCCACAGT‑TTTGCTGTGTTCTCTGGGGGAGGGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAA‑CCGTGGTTCTTGCCGAGCAGCGCGTgg < 6:223492/148‑1 (MQ=37) aCCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGG‑TTTGCTGAGCTCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAA‑CCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCt < 8:271468/148‑1 (MQ=255) tGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGG‑TTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAA‑CCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGa < 4:233596/147‑1 (MQ=255) cAGCCCTTCGCGGGCACGCCCGCTCCCACAGG‑TTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGt < 1:78610/148‑1 (MQ=255) cgcgGGCACGCCCGCTCCCACAGG‑TTTGCTGAGATCTCTGGGGGTGGGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAA‑CCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATCCccacca < 2:147905/149‑1 (MQ=255) | TGGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCAGGCACGCCCGCTCCCACAGG‑TTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAA‑CCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATCCCCACCA > NC_002947/352924‑353212 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |