Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,662,807 C→T 7.2% A557A (GCC→GCT)  nuoG → NADH‑quinone oxidoreductase subunit G

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,662,8070CT 7.2% 160.8 / 2.3 55A557A (GCC→GCT) nuoGNADH‑quinone oxidoreductase subunit G
Reads supporting (aligned to +/- strand):  ref base C (23/28);  new base T (2/2);  total (25/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01

CAACATCGCCAAGGCCCTGAAGCTGCGCGAGAAAAACGGCTCGCTGAGCCTGGTGGTGCCTGAGGCCAACAGCCTCGGCCTGGCCATGCTCGGTGGCGAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGATGCCATCGTGGTGCTGGAAAACGACCTGTACGCCCGCGTACCGGCCGCCAAGGTCGATGCTGCCCTGGCTGCGGCCAAGGTGGTGATCGTTGCCGACCACTCCAAAACCGCCACCGTCGACCGCGCCCACCTGGTGCTGCCGGCCG  >  NC_002947/4662666‑4662955
                                                                                                                                             |                                                                                                                                                    
cAACATCGCCAAGGCCCTGAAGCTGCGCGAGAAAAACGGCTCGCTGAGCCTGGTGGTGCCTGAGGCCAACAGCCTCGGCCTGGCCATGCTCGGTGGCGAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGATGCCa                                                                                                                                               <  2:234796/149‑1 (MQ=255)
cAACATCGCCAAGGCCCTGAAGCTGCGCGAGAAAAACGGCTCGCTGAGCCTGGTGGTGCCTGAGGCCAACAGCCTCGGCCTGGCCATGCTCGGTGGCGAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGATGCCa                                                                                                                                               >  4:3674/1‑149 (MQ=255)
    aTCGCCAAGGCCCTGAAGCTGCGCGAGAAAAACGGCTCGCTGAGCCTGGTGGTGCCTGAGGCCAACAGCCTCGGCCTGGCCATGCTCGGTGGCGAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGATGCCAt                                                                                                                                              >  3:299566/1‑146 (MQ=255)
    aTCGCCAAGGCCCTGAAGCTGCGCGAGAAAAACGGCTCGCTGAGCCTGGTGGTGCCTGAGGCCAACAGCCTCGGCCTGGCCATGCTCGGTGGCGAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGATGCCAt                                                                                                                                              <  4:299566/146‑1 (MQ=255)
     tCGCCAAGGCCCTGAAGCTGCGCGAGAAAAACGGCTCGCTGAGCCTGGTGGTGCCTGAGGCCAACAGCCTCGGCCTGGCCATGCTCGGTGGCGAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGATGCCATCgtg                                                                                                                                          >  6:10360/1‑149 (MQ=255)
     tCGCCAAGGCCCTGAAGCTGCGCGAGAAAAACGGCTCGCTGAGCCTGGTGGTGCCTGAGGCCAACAGCCTCGGCCTGGCCATGCTCGGTGGCGAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGATGCCATCgtg                                                                                                                                          <  5:66268/149‑1 (MQ=255)
          aaGGCCCTGAAGCTGCGCGAGAAAAACGGCTCGCTGAGCCTGGTGGTGCCTGAGGCCAACAGCCTCGGCCTGGCCATGCTCGGTGGCGAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGAt                                                                                                                                                   >  8:318114/1‑135 (MQ=255)
          aaGGCCCTGAAGCTGCGCGAGAAAAACGGCTCGCTGAGCCTGGTGGTGCCTGAGGCCAACAGCCTCGGCCTGGCCATGCTCGGTGGCGAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGAt                                                                                                                                                   <  7:318114/135‑1 (MQ=255)
          aaGGCCCTGAAGCTGCGCGAGAAAAACGGCTCGCTGAGCCTGGTGGTGCCTGAGGCCAACAGCCTCGGCCTGGCCATGCTCGGTGGCGAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGATGCCATCGTGGTGCt                                                                                                                                     <  7:279856/149‑1 (MQ=255)
             gCCCTGAAGCTGCGCGAGAAAAACGGCTCGCTGAGCCTGGTGGTGCCTGAGGCCAACAGCCTCGGCCTGGCCATGCTCGGTGGCGAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGATGCCATCGTGGTGCTgg                                                                                                                                   >  8:116260/1‑148 (MQ=255)
                cTGAAGCTGCGCGAGAAAAACGGCTCGCTGAGCCTGGTGGTGCCTGAGGCCAACAGCCTCGGCCTGGCCATGCTCGGTGGCGAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGATGCCATCGTGGTGCTGGaaaa                                                                                                                               <  1:187005/149‑1 (MQ=255)
                   aaGCTGCGCGAGAAAAACGGCTCGCTGAGCCTGGTGGTGCCTGAGGCCAACAGCCTCGGCCTGGCCATGCTCGGTGGCGAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGATGCCATCGTGGTGCTGGAAAAc                                                                                                                              <  7:222163/147‑1 (MQ=255)
                    aGCTGCGCGAGAAAAACGGCTCGCTGAGCCTGGTGGTGCCTGAGGCCAACAGCCTCGGCCTGGCCATGCTCGGTGGCGAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGATGCCATCGTGGTGCTGGAAAACGAc                                                                                                                           <  5:349317/149‑1 (MQ=255)
                         cgcgAGAAAAACGGCTCGCTGAGCCTGGTGGTGCCTGAGGACAACAGCCTCGGCCTGGCCATGCTCGGTGGCGAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGATGCCATCGTGGTGCTGGAAAACGACCTGt                                                                                                                       <  4:113269/148‑1 (MQ=255)
                          gcgAGAAAAACGGCTCGCTGAGCCTGGTGGTGCCTGAGGCCAACAGCCTCGGCCTGGCCATGCTCGGTGGCGAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGATGCCATCGTGGTGCTGGAAAACGACCTGTAc                                                                                                                     <  6:176060/149‑1 (MQ=255)
                            gagaAAAACGGCTCGCTGAGCCTGGTGGTGCCTGAGGCCAACAGCCTCGGCCTGGCCATGCTCGGTGGCGAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGATGCCATCGTGGTGCTGGAAAACGACCTGTACGc                                                                                                                   <  4:157959/149‑1 (MQ=255)
                                       cTCGCTGAGCCTGGTGGTGCCTGAGGCCAACAGCCTCGGCCTGGCCATGCTCGGTGGCGAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGATGCCATCGTGGTGCTGGAAAACGACCTGTACGCCCGCGTACCgg                                                                                                        >  6:257764/1‑149 (MQ=255)
                                        tCGCTGAGCCTGGTGGTGCCTGAGGCCAACAGCCTCGGCCTGGCCATGCTCGGTGGCGAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGATGCCATCGTGGTGCTGGAAAACGACCTGTACGCCCGCGTACCGgc                                                                                                       >  5:316588/1‑149 (MQ=255)
                                        tCGCTGAGCCTGGTGGTGCCTGAGGCCAACAGCCTCGGCCTGGCCATGCTCGGTGGCGAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGATGCCATCGTGGTGCTGGAAAACGACCTGTACGCCCGCGTACCGgc                                                                                                       >  3:351430/1‑149 (MQ=255)
                                          gCTGAGCCTGGTGGTGCCTGAGGCCAACAGCCTCGGCCTGGCCATGCTCGGTGGCGAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGATGCCATCGTGGTGCTGGAAAACGACCTGTACGCGGGCGTACAggt                                                                                                       <  6:173565/147‑2 (MQ=255)
                                                          ccTGAGGCCAACAGCCTCGGCCTGGCCATGCTCGGTGGCGAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGATGCCATCGTGGTGCTGGAAAACGACCTGTACGCCCGCGTACCGGCCGCCAAGGTCGAtgctgc                                                                                     >  3:333346/1‑149 (MQ=255)
                                                          ccTGAGGCCAACAGCCTCGGCCTGGCCATGCTCGGTGGCGAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGATGCCATCGTGGTGCTGGAAAACGACCTGTACGCCCGCGTACCGGCCGCCAAGGTCGAtgctg                                                                                      <  7:116260/148‑1 (MQ=255)
                                                             gAGGCCAACAGCCTCGGCCTGGCCATGCTCGGTGGCGAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGATGCCATCGTGGTGCTGGAAAACGACCTGTACGCCCGCGTACCGGCCGCCAAGGTCGATGCTGcctt                                                                                  >  1:26134/1‑147 (MQ=255)
                                                              aGGCCAACAGCCTCGGCCTGGCCATGCTCGGTGGCGAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGATGCCATCGTGGTGCTGGAAAACGACCTGTACGCCCGCGTACCGGCCGCCAAGGTCGATGCTGCCCt                                                                                  <  5:10360/148‑1 (MQ=255)
                                                               ggCCAACAGCCTCGGCCTGGCCATGCTCGGTGGCGAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGATGCCATCGTGGTGCTGGAAAACGACCTGTACGCCCGCGTACCGGCCGCCAAGGTCGATGCTGCCCTgg                                                                                <  1:20450/149‑1 (MQ=255)
                                                                gCCAACAGCCTCGGCCTGGCCATGCTCGGTGGCGAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGATGCCATCGTGGTGCTGGAAAACGACCTGTACGCCCGCGTACCGGCCGCCAAGGTCGATGCTGcc                                                                                    >  1:290535/1‑144 (MQ=255)
                                                                gCCAACAGCCTCGGCCTGGCCATGCTCGGTGGCGAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGATGCCATCGTGGTGCTGGAAAACGACCTGTACGCCCGCGTACCGGCCGCCAAGGTCGATGCTGcc                                                                                    <  2:290535/144‑1 (MQ=255)
                                                                   aaCAGCCTCGGCCTGGCCATGCTCGGTGGCGAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGATGCCATCgtg                                                                                                                                          >  7:122960/1‑87 (MQ=255)
                                                                   aaCAGCCTCGGCCTGGCCATGCTCGGTGGCGAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGATGCCATCgtg                                                                                                                                          <  8:122960/87‑1 (MQ=255)
                                                                   aaCAGCCTCGGCCTGGCCATGCTCGGTGGCGAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGATGCCATCGTGGTGCTGGAAAACGAc                                                                                                                           >  1:118470/1‑102 (MQ=255)
                                                                   aaCAGCCTCGGCCTGGCCATGCTCGGTGGCGAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGATGCCATCGTGGTGCTGGAAAACGAc                                                                                                                           <  2:118470/102‑1 (MQ=255)
                                                                        ccTCGGCCTGGCCATGCTCGGTGGCGAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCTGATGCCATCGTGGTGCTGGAAAACGACCTGTACGCCCGCGTACCGGCCGCCAAGGTCGATGCTGCCCTGGCTGCGGcc                                                                        >  3:230998/1‑148 (MQ=255)
                                                                        ccTCGGCCTGGCCATGCTCGGTGGCGAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCTGATGCCATCGTGGTGCTGGAAAACGACCTGTACGCCCGCGTACCGGCCGCCAAGGTCGATGCTGCCCTGGCTGCGGcc                                                                        >  3:235606/1‑148 (MQ=255)
                                                                            ggCCTGGCCATGCTCGGTGGCGAGTCGGTCGATGCCGCCCTAGACGCTGTCATCAGCGGCAAAGCTGATGCCATCGTGGTGCTGGAAAACGACCTGTACGCCCGCGTACCGGCCGCCAAGGTCGATGCTGCCCTGGCTGCGGCCAAgg                                                                    <  4:235606/148‑1 (MQ=255)
                                                                            ggCCTGGCCATGCTCGGTGGCGAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCTGATGCCATCGTGGTGCTGGAAAACGACCTGTACGCCCGCGTACCGGCCGCCAAGGTCGATGCTGCCCTGGCTGCGGCCAAgg                                                                    <  4:230998/148‑1 (MQ=255)
                                                                             gCCTGGCCATGCTCGGTGGCGAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGATGCCATCGTGGTGCTGGAAAACGACCTGTACGCCCGCGTACCGGCCGCCAAGGTCGATGCTGCCTTGGCCGCAGCCAgggca                                                                  <  2:26134/149‑3 (MQ=255)
                                                                             gCCTGGCCATGCTCGGTGGCGAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGATGCCATCGTGGTGCTGGAAAACGACCTGTACGCCCGCGTACCGGCCGCCAAGGTCGATGCTGCCCTGGCTGCGGCCAAggtg                                                                  <  1:12752/149‑1 (MQ=255)
                                                                               cTGGCCATGCTCGGTGGCGAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGATGCCATCGTGGTGCTGGAAAACGACCTGTACGccc                                                                                                                 <  4:56141/100‑1 (MQ=255)
                                                                               cTGGCCATGCTCGGTGGCGAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGATGCCATCGTGGTGCTGGAAAACGACCTGTACGccc                                                                                                                 >  3:56141/1‑100 (MQ=255)
                                                                               cTGGCCATGCTCGGTGGCGAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGATGCCATCGTGGTGCTGGAAAACGACCTGTACGCCCGCGTACCGGCCGCCAAGGTCGATGCTGCCCTGGCTGCGGCCAAggtggt                                                                <  4:351430/149‑1 (MQ=255)
                                                                                    cATGCTCGGTGGCGAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGATGCCATCGTGGTGCTGGAAAACGACCTGTACGCCCGCGTACCGGCCGCCAAGGTCGATGCTGCCCTGGCTGCGGCCAAGGTGGTGATCg                                                           <  6:316588/149‑1 (MQ=255)
                                                                                           ggTGGCGAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGATGCCATCGTGGTTCTGGAAAACGACCTGTACGCCCGCGTACCGGCCGCCAAGGTCGATGCTGCCCTGGCTGCGGCCAAGGTGGTGATCGTTGCCGa                                                    <  1:162292/149‑1 (MQ=255)
                                                                                                 gAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGATGCCATCGTGGTGCTGGAAAACGACCTGt                                                                                                                       >  7:280050/1‑76 (MQ=255)
                                                                                                 gAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGATGCCATCGTGGTGCTGGAAAACGACCTGt                                                                                                                       <  8:280050/76‑1 (MQ=255)
                                                                                                 gAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGATGCCATCGTGGTGCTGGAAAACGACCTGTACGCCCGCGTACCGGCCGCCAAGGTCGATGCTGCCCTGGCTGCGGCCAAGGTGGTGATCGTTGCCGACCACTc                                              <  3:3674/149‑1 (MQ=255)
                                                                                                      ggtcgATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGATGCCATCGTGGTGCTGGAAAACGACCTGTACGCCCGCGTACCGGCCGCCAAGGTCGAtgct                                                                                       >  2:331724/1‑103 (MQ=255)
                                                                                                       gtcgATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGATGCCATCGTGGTGCTGGAAAACGACCTGTACGCCCGCGTACCGGCCGCCAAGGTCGATGCTGCCCTGGCTGCGGCCAAGGTGGTGATCGTTGCCGACCACTCCAAAAc                                        >  6:351301/1‑149 (MQ=255)
                                                                                                         cgATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGATGCCATCGTGGTGCTGGAAAACGACCTGTACGCCCGCGTACCGGCCGCCAAGGTCGATGCTGCCCTGGCTGCGGCCAAGGTGGTGATCGTTGCCGACCACTCCAAAACCg                                      >  5:45333/1‑149 (MQ=255)
                                                                                                          gATGCCGCACTAGACGCGGTCATCAGCGGCAAAGCCGATGCCATCGTGGTGCTGGAAAACGACCTGTACGCCCGCGTACCGGCCGCCAAGGTCGATGCTGCCATGGCTGCGGCCAAGGTGGTGATCGTTGCCGACGAGTGCTAAACCGc                                     >  7:142400/1‑149 (MQ=255)
                                                                                                                gccCTAGACGCGGTCATCAGCGGCAAAGCCGATGCCATCGTGGTGCTGGAAAACGACCTGTACGCCCGCGTACCGGCCGCCAAGGTCGATGCTGCCCTGGCTGCGGCCAAGGTGGTGATCGTTGCCGACCACTCCAAAACCGCCACCGt                               >  3:93229/1‑149 (MQ=255)
                                                                                                                         gcgGTCATCAGCGGCAAAGCCGATGCCATCGTGGTGCTGGAAAACGACCTGTACGCCCGCGTACCGGCCGCCAAGGTCGATGCTGCCCTGGCTGCGGCCAAGGTGGTGATCGTTACCGACCACTCCAAAACCGCCACCGTCGACCgcgc                      >  3:233903/1‑149 (MQ=255)
                                                                                                                           ggTCATCAGCGGCAAAGCCGATGCCATCGTGGTGCTGGAAAACGACCTGTACGCCCGCGTACCGGCCGCCAAGGTCGATGCTGCCCTGGCTGCGGCCAAGGTGGTGATCGTTACCGACCACTCCAAAACCGCCACCGTCGACCGCGccc                    <  4:233903/149‑1 (MQ=255)
                                                                                                                               atcaGCGGCAAAGCCGATGCCATCGTGGTGCTGGAAAACGACCTGTACGCCCGCGTACCGGCCGCCAAGGTCGATGCTGCCCTGGCTGCGGCCAAGGTGGTGATCGTTGCCGACCACTCCAAAACCGCCACCGTCGACCGCGCCCAcc                 <  7:31839/148‑1 (MQ=255)
                                                                                                                                tcaGCGGCAAAGCCGATGCCATCGTGGTGCTGGAAAACGACCTGTACGCCCGCGTACCGGCCGCCAAGGTCGATGCTGCCCTGGCTGCGGCCAAGGTGGTGATCGTTGCCGACCACTCCAAAACCGCCACCGTCGACCGCGCCCACCTg               >  6:180467/1‑149 (MQ=255)
                                                                                                                                  aGCGGCAAAGCCGATGCCATCGTGGTGCTGGAAAACGACCTGTACGCCCGCGTACCGGCCGCCAAGGTCGATGCTGCCCTCGCTGCGGCCAAGGTGGTGATCGTTGCCGACAACTCCAAAACCGACAACGTCGACAGCGCCCACCtctt             >  2:216069/1‑146 (MQ=255)
                                                                                                                                             cGATGCCATCGTGGTGCTGGAAAACGACCTGTACGCCCGCGTACCGGCCGCCAAGGTCGATGCTGCCCTGGCTGCGGCCAAGGTGGTGATCGTTGCCGACCACTCCAAAACCGCCACCGTCGACCGCGCCCACCTGGTGCTgccggccg  >  6:8108/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                    
CAACATCGCCAAGGCCCTGAAGCTGCGCGAGAAAAACGGCTCGCTGAGCCTGGTGGTGCCTGAGGCCAACAGCCTCGGCCTGGCCATGCTCGGTGGCGAGTCGGTCGATGCCGCCCTAGACGCGGTCATCAGCGGCAAAGCCGATGCCATCGTGGTGCTGGAAAACGACCTGTACGCCCGCGTACCGGCCGCCAAGGTCGATGCTGCCCTGGCTGCGGCCAAGGTGGTGATCGTTGCCGACCACTCCAAAACCGCCACCGTCGACCGCGCCCACCTGGTGCTGCCGGCCG  >  NC_002947/4662666‑4662955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑