Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,682,236 G→A 7.8% D176N (GAC→AAC)  mltD → membrane‑bound lytic murein transglycosylase D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,682,2360GA 7.8% 181.7 / 4.7 64D176N (GAC→AAC) mltDmembrane‑bound lytic murein transglycosylase D
Reads supporting (aligned to +/- strand):  ref base G (30/29);  new base A (2/3);  total (32/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.30e-01

ATGCCGCTGGAACTGGCCCTGCTGCCAGCCATCGAAAGTGCCTACAACCCCATGGCCTACTCACGCGCCAGCGCTGCCGGCATGTGGCAGTTCATGCCGGCTACCGGTCGCCACTTCAACCTGCGCCAGACCAACTTCTATGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGACCCGCCTGCACGACATGTTCAACGGCGACTGGCTGTTGGCCCTGGCGGCCTACAATGCCGGCGAAGGCACCGTCAGCCGAGCCATCGAGCGCAACG  >  NC_002947/4682095‑4682383
                                                                                                                                             |                                                                                                                                                   
aTGCCGCTGGAACTGGCCCTGCTGCCAGCCATCGAAAGTGCCTACAACCCCATGGCCTACTCACGCGCCAGCGCTGCCGGCATGTGGCAGTTCATGCCGGCTACCGGTCGCCACTTCAACCTGCGCCAGACCAACTTCTATGACGgccg                                                                                                                                              >  1:141571/1‑149 (MQ=255)
  gCCGCTGGAACTGGCCCTGCTGCCAGCCATCGAAAGTGCCTACAACCCCATGGCCTACTCACGCGCCAGCGCTGCCGGCATGTGGCAGTTCATGCCGGCTACCGGTCGCCACTTCAACCTGCGCCAGACCAACTTCTATGACGgccgcc                                                                                                                                            >  8:192370/1‑149 (MQ=255)
     gCTGGAACTGGCCCTGCTGCCAGCCATCGAAAGTGCCTACAACCCCATGGCCTACTCACGCGCCAGCGCTGCCGGCATGTGGCAGTTCATGCCGGCTACCGGTCGCCACTTCAACCTGCGCCAGACCAACTTCTATGACGGCCGCcgcg                                                                                                                                         <  6:65611/149‑1 (MQ=255)
         gAACTGGCCCTGCTGCCAGCCATCGAAAGTGCCTACAACCCCATGGCCTACTCACGCGCCAGCGCTGCCGGCATGTGGCAGTTCATGCCGGCTACCGGTCGCCACTTCAATCTGCGCCAGACCAACTTCTATGACGGCCGCCGCGAt                                                                                                                                       <  1:57396/147‑1 (MQ=255)
         gAACTGGCCCTGCTGCCAGCCATCGAAAGTGCCTACAACCCCATGGCCTACTCACGCGCCAGCGCTGCCGGCATGTGGCAGTTCATGCCGGCTACCGGTCGCCACTTCAACCTGCGCCAGACCAACTTCTATGACGGCCGCCGCGATGt                                                                                                                                     <  7:192370/149‑1 (MQ=255)
              ggCCCTGCTGCCAGCCATCGAAAGTGCCTACAACCCCATGGCCTACTCACGCGCCAGCGCTGCCGGCATGTGGCAGTTCATGCCGGCTACCGGTCGCCACTTCAACCTGCGCCAGACCAACTTCTATGACGGCCGCCGCGATGTCACcg                                                                                                                                >  2:116954/1‑149 (MQ=255)
                     ctgcCAGCCATCGAAAGTGCCTACAACCCCATGGCCTACTCACGCGCCAGCGCTGCCGGCATGTGGCAGTTCATGCCGGCTACCGGTCGCCACTTCAACCTGCGCCAGACCAACTTCTATGACGGCCGCCGCGATGTCACCGCGTCGAc                                                                                                                         <  5:233521/149‑1 (MQ=255)
                       gccagccaTCGAAAGTGCCTACAACCCCATGGCCTACTCACGCGCCAGCGCTGCCGGCATGTGGCAGTTCATGCCGGCTACCGGTCGCCACTTCAACCTGCGCCAGACCAACTTCTATGACGGCCGCCGCGATGTCACCGCGTCGAcc                                                                                                                        >  7:123258/1‑148 (MQ=255)
                        ccagccaTCGAAAGTGCCTACAACCCCATGGCCTACTCACGCGCCAGCGCTGCCGGCATGTGGCAGTTCATGCCGGCTACCGGTCGCCACTTCAACCTGCGCCAGACCAACTTCTATGACGGCCGCCGCGATGTCACCGCGTCGACCaa                                                                                                                      >  7:174384/1‑149 (MQ=255)
                          agccaTCGAAAGTGCCTACAACCCCATGGCCTACTCACGCG‑CAGCGCTGCCGGCATGTGGCAGTTCATGCCGGCTACCGGTCGCCACTTCACACTGCGCCATACCAACTTCTATGACGTCCGCCGCGATGTCACCGCGTCGCCCAAcga                                                                                                                   >  4:229918/1‑148 (MQ=255)
                              aTCGAAAGTGCCTACAACCCCATGGCCTACTCACGCGCCAGCGCTGCCGGCATGTGGCAGTTCATGCCGGCTACCGGTCGCCACTTCAACCTGCGCCAGACCAACTTCTATGACGGCCGCCGCGATGTCACCGCGTCGACCAAcgccgc                                                                                                                >  3:76551/1‑149 (MQ=255)
                                 gAAAGTGCCTACAACCCCATGGCCTACTCACGCGCCAGCGCTGCCGGCATGTGGCAGTTCATGCCGGCTACCGGTCGCCACTTCAACCTGCGCCAGACCAACTTCTATGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCt                                                                                                             >  4:285705/1‑149 (MQ=255)
                                  aaaGTGCCTACAACCCCATGGCCTACTCACGCGCCAGCGCTGCCGGCATGTGGCAGTTCATGCCGGCTACCGGTCGCCACTTCAACCTGCGCCAGACCAACTTCTATGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTg                                                                                                            <  8:174384/149‑1 (MQ=255)
                                  aaaGTGCCTACAACCCCATGGCCTACTCACGCGCCAGCGCTGCCGGCATGTGGCAGTTCATGCCGGCTACCGGTCGCCACTTCAACCTGCGCCAGACCAACTTCCATGACGGCCGCCGCGATGTCACCGCGTCGAcc                                                                                                                        <  7:23544/137‑1 (MQ=255)
                                  aaaGTGCCTACAACCCCATGGCCTACTCACGCGCCAGCGCTGCCGGCATGTGGCAGTTCATGCCGGCTACCGGTCGCCACTTCAACCTGCGCCAGACCAACTTCCATGACGGCCGCCGCGATGTCACCGCGTCGAcc                                                                                                                        >  8:23544/1‑137 (MQ=255)
                                   aaGTGCCTACAACCCCATGGCCTACTCACGCGCCAGCGCTGCCGGCATGTGGCAGTTCATGCCGGCTACCGGTCGCCACTTCAACCTGCGCCAGACCAACTTCTATGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTgg                                                                                                           <  8:79202/149‑1 (MQ=255)
                                              aCCCCATGGCCTACTCACGCGCCAGCGCTGCCGGCATGTGGCAGTTCATGCCGGCTACCGGTCGCCACTTCAACCTGCGCCAGACCAACTTCTATGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGAcc                                                                                                <  5:365529/149‑1 (MQ=255)
                                                  cATGGCCTACTCACGCGCCAGCGCTGCCGGCATGTGGCAGTTCATGCCGGCTACCGGTCGCCACTTCAACCTGCGCCAGACCAACTTCTATGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGACCCGcc                                                                                            >  4:290136/1‑149 (MQ=255)
                                                   aTGGCCTACTCACGCGCCAGCGCTGCCGGCATGTGGCAGTTCATGCCGGCTACCGGTCGCCACTTCAACCTGCGCCAGACCAACTTCTATGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGACCCGCCt                                                                                           >  6:71467/1‑149 (MQ=255)
                                                   aTGGCCTACTCACGCGCCAGCGCTGCCGGCATGTGGCAGTTCATGCCGGCTACCGGTCGCCACTTCAACCTGCGCCAGACCAACTTCTATAACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGACCCGCCt                                                                                           <  7:20046/149‑1 (MQ=255)
                                                           cTCACGCGCCAGCGCTGCCGGCATGTGGCAGTTCATGCCGGCTACCGGTCGCCACTTCAACCTGCGCCAGACCAACTTCTATGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGAc                                                                                                 >  8:293974/1‑135 (MQ=255)
                                                           cTCACGCGCCAGCGCTGCCGGCATGTGGCAGTTCATGCCGGCTACCGGTCGCCACTTCAACCTGCGCCAGACCAACTTCTATGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGAc                                                                                                 <  7:293974/135‑1 (MQ=255)
                                                              aCGCGCCAGCGCTGCCGGCATGTGGCAGTTCATGCCGGCTACCGGTCGCCACTTCAACCTGCGCCAGACCAACTTCTATGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGACCCGCCTGCACGACATGt                                                                                >  7:258500/1‑149 (MQ=255)
                                                              aCGCGCCAGCGCTGCCGGCATGTGGCAGTTCATGCCGGCTACCGGTCGCCACTTCAACCTGCGCCAGACCAACTTCTATGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCCCGCTGGACTACCTGACCCGCCTGCACGACATg                                                                                 >  7:56946/1‑148 (MQ=255)
                                                                   ccAGCGCTGCCGGCATGTGGCAGTTCATGCCGGCTACCGGTCGCCACTTCAACCTGCGCCAGACCAACTTCTATGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGACCCGCCTGCACGACATGTTCAAc                                                                           <  8:123258/149‑1 (MQ=255)
                                                                      gcgcTGCCGGCATGTGGCAGTTCATGCCGGCTACCGGTCGCCACTTCAACCTGCGCCAGACCAACTTCTATGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGACCCGCCTGCACGACATGTTCAACGGc                                                                        >  3:240988/1‑149 (MQ=255)
                                                                      gcgcTGCCGGCATGTGGCAGTTCATGCCGGCTACCGGTCGCCACTTCAACCTGCGCCAGACCAACTTCTATGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGACCCGCCTGCACGACATGTTCAACGGc                                                                        <  3:151276/149‑1 (MQ=255)
                                                                                      gCAGTTCATGCCGGCTACCGGTCGCCACTTCAACCTGCGCCAGACCAACTTCTATGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGACCCGCCTGCACGACATGTTCAACg                                                                          >  1:172832/1‑131 (MQ=255)
                                                                                      gCAGTTCATGCCGGCTACCGGTCGCCACTTCAACCTGCGCCAGACCAACTTCTATGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGACCCGCCTGCACGACATGTTCAACg                                                                          <  2:172832/131‑1 (MQ=255)
                                                                                      gCAGTTCATGCCGGCTACCGGTCGCCACTTCAACCTGCGCCAGACCAACTTCTATGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGACCCGCCTGCACGACATGTTCAACAGCCAGTCGCCGTTgaac                                                         <  8:258500/148‑4 (MQ=255)
                                                                                          ttCATGCCGGCTACCGGTCGCCACTTCAACCTGCGCCATACCAACTTCTATGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGACCCGCCTGCACGACATGTTCAACGGCGACTGGCTGTTGGCCCTggc                                                    <  3:229918/149‑1 (MQ=255)
                                                                                           tCATGCCGGCTACCGGTCGCCACTTCAACCTGCGCCAGACCAACTTCTATGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGACCCGCCTGCACGACATGTTCAACGGCGACTGGCTGTTGGCCCTggcg                                                   >  3:232849/1‑149 (MQ=255)
                                                                                             aTGCCGGCTACCGGTCGCCACTTCAACCTGCGCCAGACCAACTTCTATAACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGACCCGCCTGCACGAc                                                                                    >  3:105182/1‑114 (MQ=255)
                                                                                             aTGCCGGCTACCGGTCGCCACTTCAACCTGCGCCAGACCAACTTCTATAACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGACCCGCCTGCACGAc                                                                                    <  4:105182/114‑1 (MQ=255)
                                                                                             aTGCCGGCTACCGGTCGCCACTTCAACCTGCGCCAGACCAACTTCTATAACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGACCCGCCTGCACGACATGTTCAACGGCGACTGGCTGTTGGCCCTggcggc                                                 <  7:123810/149‑1 (MQ=255)
                                                                                              tGCCGGCTACCGGTCGCCACTTCAACCTGCGCCAGACCAACTTCTATGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGACCCGCCTGCACGACATGTTCAACGGCGACTGGCTGTTGGCCCTGGCGGcc                                                <  3:285705/149‑1 (MQ=255)
                                                                                              tGCCGGCTACCGGTCGCCACTTCAACCTGCGCCAGACCAACTTCTATGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGACCCGCCTGCACGACATGTTCAACGGCGACTGGCTGTTGGCCCTGGCGGcc                                                >  1:14039/1‑149 (MQ=255)
                                                                                                       ccGGTCTCCACTTCAACCTGCGCCAGACCAACTTCTATGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGACCCTCCTGCACGACATGTTCAACGGCGACTGGCTGTTGGCCCTGGCGGCCTACAATGcc                                       <  5:71467/149‑1 (MQ=255)
                                                                                                       ccGGTCGCCACTTCAACCTGCGCCAGACCAACTTCTATGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGACCCGCCTGCACGACATGTTCAACGGCGACTGGCTGTTGGCCCTGGCGGCCTACAATGc                                        <  4:240988/148‑1 (MQ=255)
                                                                                                        cGGTCGCCACTTCAACCTGCGCCAGACCAACTTCTATGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGACCCGCCTGCACGACATGTTCAACGGCGACTGGCTGTTGGCCCTGGCGGCCTACAAt                                          >  3:70850/1‑145 (MQ=255)
                                                                                                        cGGTCGCCACTTCAACCTGCGCCAGACCAACTTCTATGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGACCCGCCTGCACGACATGTTCAACGGCGACTGGCTGTTGGCCCTGGCGGCCTACAAt                                          <  4:70850/145‑1 (MQ=255)
                                                                                                          gTCGCCACTTCAACCTGCGCCAGACCAACTTCTATGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGACCCGCCTGCACGACATGTTCAACGGCGACTGGCTGTTGGCCCTGGCGGCCTACAATGCCGGc                                    >  5:141058/1‑149 (MQ=255)
                                                                                                             gCCACTTCAACCTGCGCCAGACCAACTTCTATGACGGCCGCCGCGATGTCACCGCGTCGCCCAACGCCGCGCTGGACTACCTGACCCGCCTGCACGACATGTTCAACGGCGACTGGCTGTTGGCCCTGGCGGCCTACAATGCCGGCg                                   >  1:118720/1‑147 (MQ=255)
                                                                                                             gCCACTTCAACCTGCGCCAGACCAACTTCTATGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGACCCGCCTGCACGACATGTTCAACGGCGACTGGCTGTTGGCCCTGGCGGCCTACAATGCCGGCg                                   >  7:76653/1‑147 (MQ=255)
                                                                                                             gCCACTTCAACCTGCGCCAGACCAACTTCTATGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGACCCGCCTGCACGACATGTTCAACGGCGACTGGCTGTTGGCCCTGGCGGCCTACAATGCCGGCGaa                                 <  4:76551/149‑1 (MQ=255)
                                                                                                             gCCACTTCAACCTGCGCCAGACCAACTTCTATGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGACCCGCCTGCACGACATGTTAAACGGCGACTGGCTGTTGGCCCTGGCGGCCTACAATGACGGCg                                   >  7:70697/1‑147 (MQ=255)
                                                                                                              ccACTTCAACCTGCGCCAGACCAACTTCTATGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGACCCGCCTGCACGACATGTTCAACGGCGACTGGCTGTTGGCCCTGGCGGCCTACAATGCCGGCGAAg                                >  7:293995/1‑149 (MQ=255)
                                                                                                               cACTTCAACCTGCGCCAGACCAACTTCTATGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGACCCGCCTGCACGACATGTTCAACGGCGACTGGCTGTTGGCCCTGGCGGCCTACAATGCCGGCGAAgg                               >  2:55504/1‑149 (MQ=255)
                                                                                                                        cTGCGCCAGACCAACTTCTATGACGGCCGCCTCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGACCCGCCTGCACGACATGTTCAACGGCGACTGGCTGTTGGCCCTGGCGGCCTACAATGCCGGCGAAGGCACCGTCAg                      <  6:84340/149‑1 (MQ=255)
                                                                                                                        cTGCGCCAGACCAACTTCTATGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGACCCGCCTGCACGACATGTTCAACGGCGACTGGCTGTTGGCCCTGGCGGCCTACAATGCCGGCGAAGGCACCGTCAg                      <  8:76653/149‑1 (MQ=255)
                                                                                                                          gcgcCAGACCAACTCCTATGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGACCCGCCTGCACGACATGTTCAACGGCGACTGGCTGTTGGCCCTGGCGGCCTACAATGCCGGCGAAGGCACCGTCAGcc                    <  5:264229/149‑1 (MQ=255)
                                                                                                                           cgcCAGACCAACTTCTATGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGACCCGCCTGCACGACATGTTCAACGGCGACTGGCTGTTGGCCCTGGCGGCCTACAATGCCGGCGAAGGCACCGTCAGcc                    <  3:290136/148‑1 (MQ=255)
                                                                                                                            gcCAGACCAACTTCTATGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGACCCGCCTGCACGACATGTTCAACGGCGACTGGCTGTTGGCCCTGGCGGCCTACAATGCCGGCGAAg                                >  7:259115/1‑135 (MQ=255)
                                                                                                                            gcCAGACCAACTTCTATGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGACCCGCCTGCACGACATGTTCAACGGCGACTGGCTGTTGGCCCTGGCGGCCTACAATGCCGGCGAAg                                <  8:259115/135‑1 (MQ=255)
                                                                                                                            gcCAGACCAACTTCTATGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGACCCGCCTGCACGACATGTTCAACGGCGACTGGCTGTTGGCCCTGGCGGCCTACAATGCCGGCGAAGGCACCGTCAGcc                    >  3:164955/1‑147 (MQ=255)
                                                                                                                             ccAGACCAACTTCTATGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGACCCGCCTGCACGACATGTTCAACGGCGACTGGCTGTTGGCCCTGGCGGCCTACAATGCCGGCGAAGGCACCGTCAGCCGa                  <  8:56946/148‑1 (MQ=255)
                                                                                                                              cAGACCAACTTCTATGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGACCCGCCTGCACGACATGTTCAACGGCGACTGGCTGTTGGCCCTGGCGGCCTACAATGCCGGCGAAGGCACCGTCAGCCGAGc                >  4:70569/1‑149 (MQ=255)
                                                                                                                                gACCAACTTCTATAACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGACCCGCCTGCACGACATGTTCAACGGCGACTGGCTGTTGGCCCTGGCGGCCTACAATGCCGGCGAAGGCACCGTCAGCCGAGCCa              >  2:265243/1‑149 (MQ=255)
                                                                                                                                       ttCTATGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGACCCGCCTGCACGACATGTTCAACGGCGACTGGCTGTTGGCCCTGGCGGCCTACAATGCCGGCGAAGGCACCGTCAGCCGAGCCATCGAgcg       <  8:95150/149‑1 (MQ=255)
                                                                                                                                          tATGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGACCCGCCTGCACGACATGTTCAACGGCGACTGGCTGTTGGCCCTGGCGGCCTACAATGCCGGCGAAGGCACCGTCAGCCGAGCCATCGAGCGCaa    >  4:119900/1‑149 (MQ=255)
                                                                                                                                            tGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGACCCGCCTGCACGACATGTTCAACGGCGACTGGCTGTTGGCCCTGGCGGCCTACAATGCCGGCGAAGGc                              >  8:210802/1‑121 (MQ=255)
                                                                                                                                            tGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGACCCGCCTGCACGACATGTTCAACGGCGACTGGCTGTTGGCCCTGGCGGCCTACAATGCCGGCGAAGGc                              <  7:210802/121‑1 (MQ=255)
                                                                                                                                            tGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGACCCGCCTGCACGACATGTTCAACGGCGACTGGCTGTTGGCCCTGGCGGCCTACAATGCCGGCGAAGGCACCGTCAGCCGAGCCATCGAGCGCaa    <  1:116954/147‑1 (MQ=255)
                                                                                                                                            tGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGACCCGCCTGCACGACATGTTCAACGGCGACTGGCTGTTGGCCCTGGCGGCCTACAATGCCGGCGAAGGCACCGTCAGCCGAGCCATCGAGCGCAACg  <  6:141058/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                   
ATGCCGCTGGAACTGGCCCTGCTGCCAGCCATCGAAAGTGCCTACAACCCCATGGCCTACTCACGCGCCAGCGCTGCCGGCATGTGGCAGTTCATGCCGGCTACCGGTCGCCACTTCAACCTGCGCCAGACCAACTTCTATGACGGCCGCCGCGATGTCACCGCGTCGACCAACGCCGCGCTGGACTACCTGACCCGCCTGCACGACATGTTCAACGGCGACTGGCTGTTGGCCCTGGCGGCCTACAATGCCGGCGAAGGCACCGTCAGCCGAGCCATCGAGCGCAACG  >  NC_002947/4682095‑4682383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑