| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,711,617 | G→A | 6.9% | intergenic (‑76/‑152) | gpsA ← / → PP_4171 | glycerol‑3‑phosphate dehydrogenase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,711,617 | 0 | G | A | 6.9% | 165.7 / 2.3 | 58 | intergenic (‑76/‑152) | gpsA/PP_4171 | glycerol‑3‑phosphate dehydrogenase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (29/25); new base A (2/2); total (31/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.20e-01 | |||||||||||
GCCAGCAGGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACC‑AGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATCGAGCACCGCCACGCGGTACTTTTCAAGACGTACGCCCGACACCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGC > NC_002947/4711471‑4711765 | gCCAGCAGGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACC‑AGTGTCTGGa > 1:136337/1‑149 (MQ=255) tttGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACC‑AGTGTCTGGACGTTTGCgg < 6:103072/149‑1 (MQ=255) ccACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACC‑AGTGTCTGGACGTTTGCGGTACt > 3:308367/1‑149 (MQ=255) cACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGCACC‑AGTGACTGGACGTTTGCGGTACtt > 3:266932/1‑149 (MQ=255) gcggTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACC‑AGTGTCTGGACGTTTGCgg > 6:89061/1‑140 (MQ=255) gcggTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACC‑AGTGTCTGGACGTTTGCgg < 5:89061/140‑1 (MQ=255) gcggTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACC‑AGTGTCTGGACGTTTGCGGTACTTGCCa < 2:93055/149‑1 (MQ=255) gAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACC‑AGTGTCTGGACGTTTGCGGTACTTGCCATGGCAttt > 7:93259/1‑149 (MQ=255) gAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACC‑AGTGTCTGGACGTTTGCGGTACTTGCCATGGCAttt > 1:7169/1‑149 (MQ=255) cgccCAGAACCGCAACAGCTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGATAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACC‑AGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTc > 2:230240/1‑149 (MQ=255) cccAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACC‑AGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCga < 4:158932/149‑1 (MQ=255) cccAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACC‑AGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCga < 8:93259/149‑1 (MQ=255) cAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACC‑AGCGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTc > 5:221436/1‑145 (MQ=255) gCTTTTCAGTCATATCCAATCCATTAACGACACAAGTTAAGTTTCTACCCGGCCATTATACGGATCGACTGCTACAGAACA‑AGTGTCTGGACGTTTGAGGTACTTGCCTTGTCATTTTCTGAGCCTTCGAGATCGAGAACCGGCAcgc < 7:303707/148‑1 (MQ=255) gCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACC‑AGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATCGAGCACCGCCAcgc < 2:136337/148‑1 (MQ=255) cTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACC‑AGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATCGAGCACCG‑CACGCgg < 4:308367/148‑1 (MQ=255) gTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACC‑AGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATCGAGCACCGCCACGCGGTa > 7:216003/1‑149 (MQ=255) tCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACC‑AGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGGGATCGAGCACCGCCACGCGGTACtttt > 1:99630/1‑148 (MQ=255) atatCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACC‑AGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATCGAGCACCGCCACGCGGTACTTTTCa < 5:224983/148‑1 (MQ=255) atatCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACC‑AGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATCGAGCACCGCCACGCGGTACTTTTCa > 6:224983/1‑148 (MQ=255) tccaatccaTTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACC‑AGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATCGAGCACCGCCACGCGGTACTTTTCAAGAc > 3:349841/1‑149 (MQ=255) ccaatccaTTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTGCAGAACC‑AGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATCGAGCACCGCCACGCGGTACTTTTCAAGACg > 7:340754/1‑149 (MQ=255) caatccaTTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACC‑AGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATCGAGCACCGCCACGCGGTACTTTTCAAGACg > 8:113976/1‑148 (MQ=255) caatccaTTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACC‑AGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATCGAGCACCGCCACGCGGTACTTTTCAAGACg > 3:229629/1‑148 (MQ=255) aTTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACC‑AGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATCGAGCACCGCCACGCGGTACTTTTCAAGACGTACGccc > 3:137680/1‑149 (MQ=255) aTTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACC‑AGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATCGAGCACCGCCACGCGGTACTTTTCAAGACGTACGccc > 7:6577/1‑149 (MQ=255) ttAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACC‑AGTGTCTAGACGTTTGCGGTACtt < 1:328709/82‑1 (MQ=255) ttAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACC‑AGTGTCTAGACGTTTGCGGTACtt > 2:328709/1‑82 (MQ=255) ttAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACC‑AGTGTCTAGACGTTTGCGGTACtt < 1:324342/82‑1 (MQ=255) ttAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCAAAAGAACC‑AGTGTCTAGACCTATGCGGTAcat > 2:324342/1‑80 (MQ=255) aaGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACC‑AGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATCGAGCACCGCCACGCGGTACTTTTCAAGACGTACGCCCGaca > 2:39373/1‑149 (MQ=255) aaGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACC‑AGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATCGAGCACCGCCACGCGGTACTTTTCAAGACGTACGCCCGaca > 7:178867/1‑149 (MQ=255) ccAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACC‑AGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATCGAGCACCGCCACGCGGTACTTTTCAAGACGTACGCCCGacac < 5:156144/147‑1 (MQ=255) ccAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACC‑AGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATCGAGCACCGCCACGCGGTACTTTTCAAGACGTACGCCCGacac > 6:156144/1‑147 (MQ=255) aaaaGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACC‑AGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATCGAGCCCCGCCACGCGGTACTTTTCAAGACGTACGCCCGACACCCAc > 7:73947/1‑149 (MQ=255) aGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACC‑AGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATCGAGCACCGCCACGCGGTACTTTTCAAGACGTACGCCCGACACCCACTgg < 4:349841/149‑1 (MQ=255) gTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACC‑AGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATCGAGCACCGCCACGCGGTAGTTTTCAAGACGTACGCCCGACACCCACTGGCAAAAc < 1:230240/149‑1 (MQ=255) tGGCGACCCGGCCATTATACGGCTCGCCTGCTGCAGAACC‑AGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATCGAGCACCGCCACGCGGTACTTTTCAAGACGTACGCCCGACACCCACTGGCAAAAcc < 8:340754/149‑1 (MQ=255) cGACCCGGCCATTATACGGCTCGCCTGCTACAGAACC‑AGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATCGAGCACCGCCACGCGGTACTTTTCAAGACGTACGCCCGACACCCACTGGCAAAAccccc < 8:6577/149‑1 (MQ=255) ccGGCCATTATACGGCTCGCCTGCTACAGAACC‑AGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATCGAGCACCGCCACGCGGTACTTTTCAAGACGTACGCCCGACACCCACTGGCAAAACCCCCACgg > 1:43961/1‑149 (MQ=255) cGGCCATTATACGGCTCGCCTGCTACAGAACC‑AGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGGGATCGAGCACCGCCACGCGGTACTTTTCAAGACGTACGCCCGACACCCACTGGCAAAACCCCCACGGt < 2:99630/149‑1 (MQ=255) ggCCATTATACGGCTCGCCTGCTACAGAACC‑AGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATCGAGCAACGCCACGCGGTACTTTTCAAGACGTACGCCCGACACCCACTGGCAAAACCCCCACGGt > 1:171580/1‑148 (MQ=255) gCCATTATACGGCTCGCCTGCTACAGAACC‑AGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATCGAGCACCGCCACGCGGTACTTTTCAAGACGTACGCCCGACACCCACTGGCAAAACCCCCACGGTCa < 4:137680/149‑1 (MQ=255) cATTATACGGCTCGCCTGCTACAGAACC‑AGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATCGAGCACCGCCACGCGGTACTTTTCAAGACGTACGCCCGACACCCACTGGCAAAACCCCCACGGTCAGt < 4:229629/149‑1 (MQ=255) aTTATACGGCTCGCCTGCTACAGAACCGAGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATCGAGCACCGCCACGCGGTACTTTTCAAGACGTACGCCCGACACCCACTGGCAAAACCCCCACGGTCAGt > 1:199492/1‑149 (MQ=255) cTCGCCTGCTACAGAACC‑AGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATCGAGCACCGCCACGCGGTACTTTTCAAGACGTACGCCCGACACCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCt < 2:171580/149‑1 (MQ=255) gCCTGCTACAGAACC‑AGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATCGAGCACCGCCACGCGGTACTTTTCAAGACGTACGCCCGACACCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGtt < 2:7169/149‑1 (MQ=255) tGCTACAGAACC‑AGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATCGAGCACCGCCACGCGGTACTTTTCAAGACGTACGCCCGACACCCACTGGCAAAACCCCAACGGTCAGTTACCATGCCTGTTTc > 5:328041/1‑148 (MQ=255) gCTACAGAACC‑AGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATCGAGCACCGCCACGCGGTACTTTTCAAGACGTACGCCCGACACCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCaa > 8:148715/1‑149 (MQ=255) cTACAGAACC‑AGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATCGAGCACCGCCACGCGGTACTTTTCAAGACGTACGCCCGACACCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCaa < 6:328041/148‑1 (MQ=255) aCAGAACC‑AGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATCGAGCACCGCCACGCGGTACTTTTCAAGACGTACGCCCGACACCCACTGGCAAAACCCCCACGGTCAGtt < 5:281811/130‑1 (MQ=255) aCAGAACC‑AGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATCGAGCACCGCCACGCGGTACTTTTCAAGACGTACGCCCGACACCCACTGGCAAAACCCCCACGGTCAGtt > 6:281811/1‑130 (MQ=255) cAGAACC‑AGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATCGAGCACCGCCACGCGGTACTTTTCAAGACGTACGCCCGACACCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCt > 3:282075/1‑149 (MQ=255) cAGAACC‑AGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATCGAGCACCGCCACGCGGTACTTTTCAAGACGTACGCCCGACACCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCt < 2:43961/149‑1 (MQ=255) aGAACC‑AGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATCGAGCACCGCCACGCGGTACTTTTCAAGACGTACGCCCGACACCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCt > 7:201948/1‑148 (MQ=255) gtgtCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATCGAGCACCGCCACGCGGTACTTTTCAAGACGTACGCCCGACACCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACaaa < 7:113976/149‑1 (MQ=255) gtgtCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATCGAGCACCGCCACGCGGTACTTTTCAAGACGTACGCCCGACACCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACaaa < 8:178867/149‑1 (MQ=255) tgtCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATCGAGCACCGCCACGCGGTACTTTTCAAGACGTACGCCCGACACCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAg < 8:201948/149‑1 (MQ=255) ggACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATCGAGCACCGCCACGCGGTACTTTTCAAGACGTACGCCCGACACCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGc > 4:341952/1‑149 (MQ=255) | GCCAGCAGGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACC‑AGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATCGAGCACCGCCACGCGGTACTTTTCAAGACGTACGCCCGACACCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGC > NC_002947/4711471‑4711765 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |