| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,725,040 | G→A | 14.2% | intergenic (+25/+43) | PP_5661 → / ← PP_4183 | putative lipoprotein/membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,725,040 | 0 | G | A | 14.2% | 130.7 / 12.8 | 57 | intergenic (+25/+43) | PP_5661/PP_4183 | putative lipoprotein/membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (24/24); new base A (3/5); total (27/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.07e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.47e-01 | |||||||||||
AAGCCGCCGAGGAAAACGCCGAAGCTGCCAAAGATCAGGCCGAAGCCGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTC‑‑GGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAGTACACTTTCGGTCGGTGTGAACACCATCACATCGAGTACATCCGAATGGAACTCTCGACGG > NC_002947/4724894‑4725185 | aaGCCGCCGAGGAAAACGCCGAAGCTGCCAAAGATCAGGCCGAAGCCGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGAt > 6:198688/1‑149 (MQ=255) ccgAGGAAAACGCCGAAGCTGCCAAAGATCAGGCCGAAGCCGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCACCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGAAGTC‑‑ggg > 3:219029/1‑149 (MQ=255) gAGGAAAACGCCGAAGCTGCCAAAGATCAGGCCGAAGCCGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACATCGTC‑‑GGGtt < 6:353731/149‑1 (MQ=255) gAAAACGCCGAAGCTGCCAAAGATCAGGCCGAAGCCGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTC‑‑GGGttttt > 1:291371/1‑149 (MQ=255) aaaCGCCGAAGCTGCCAAAGATCAGGCCGAAGCCGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTC‑‑GGGttttttt < 1:214238/149‑1 (MQ=255) aaaCGCCGAAGCTGCCAAAGATCAGGCCGAAGCCGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTC‑‑GGGttttttt > 8:245697/1‑149 (MQ=255) aaCGCCGAAGCTGCCAAAGATCAGGCCGAAGCCGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTC‑‑GGGTTTTTTtg < 2:291371/149‑1 (MQ=255) cGCCGAAGCTGCCAAAGATCAGGCCGAAGCCGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTC‑‑GGGTTTTTTtgtg > 8:132704/1‑149 (MQ=255) cGAAGCTGCCAAAGATCAGGCCGAAGCCGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTC‑‑GGGTTTTTTTGTGCCt > 5:24291/1‑149 (MQ=255) cGAAGCTGCCAAAGATCAGGCCGAAGCCGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACATCGTC‑‑GGGTTTTTTtgtttta < 1:209555/149‑5 (MQ=255) cAAAGATCAGGCCGAAGCCGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTC‑‑GGGTTTTTTTGTGCCTGCGAACttt > 3:154237/1‑149 (MQ=255) cAAAGATCAGGCCGAAGCCGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTC‑‑GGGTTTTTTTGTGCCTGCGAACttt > 5:268522/1‑149 (MQ=255) cAAAGATCAGGCCGAAGCCGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACATCGTC‑‑GGGttttttt > 7:322460/1‑134 (MQ=255) cAAAGATCAGGCCGAAGCCGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACATCGTC‑‑GGGttttttt < 8:322460/134‑1 (MQ=255) aaaGATCAGGCCGAAGCCGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTC‑‑GGGTTTTTTTGTGCCTGCGAACTTTc < 7:132704/149‑1 (MQ=255) aaaGATCAGGCCGAAGCCGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTC‑‑GGGTTTTTTTGTGCCTGCGAACTTTc < 5:198688/149‑1 (MQ=255) aaaGATCAGGCCGAAGCCGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACATCGTC‑‑GGGTTTTTTtgtttta > 8:96907/1‑135 (MQ=255) aaaGATCAGGCCGAAGCCGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACATCGTC‑‑GGGTTTTTTtgtttta < 7:96907/139‑5 (MQ=255) aGATCAGGCCGAAGCCGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAAC‑AAAAAACCCGACGATGTC‑‑GGGt < 4:275037/124‑1 (MQ=255) aGATCAGGCCGAAGCCGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAAC‑AAAAAACCCGACGATGTC‑‑GGGt > 3:275037/1‑124 (MQ=255) cAGGCCGAAGCCGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTC‑‑GGGtttt > 3:11004/1‑124 (MQ=255) cAGGCCGAAGCCGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTC‑‑GGGtttt < 4:11004/124‑1 (MQ=255) aGGCCGAAGCCGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTC‑‑GGGTTTTTTTGTGCCTGCGAACTTTCGATTaa < 7:245697/148‑1 (MQ=255) gCCGAAGCCGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTC‑‑GGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCg > 3:230109/1‑149 (MQ=255) gAAGCCGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTC‑‑GGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGttt < 8:238867/149‑1 (MQ=255) ccGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTC‑‑GGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGt < 6:268522/149‑1 (MQ=255) gCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTC‑‑GGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTgg < 6:24291/148‑1 (MQ=255) gAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTC‑‑GGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAaac < 2:331301/149‑1 (MQ=255) aaGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTC‑‑GGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGttt > 7:111590/1‑137 (MQ=255) aaGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTC‑‑GGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGttt < 8:111590/137‑1 (MQ=255) gagCCGGAGTAGCCGGAGCGGCTTCTTCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTC‑‑GGGttttttt < 2:68636/103‑1 (MQ=37) gagCCGGAGTAGCCGGAGCGGCTTCTTCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTC‑‑GGGttttttt > 1:68636/3‑105 (MQ=37) gCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTC‑‑GGGTTTTTTTGTGTCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAg > 3:55864/1‑149 (MQ=255) agCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTC‑‑GGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCtt < 4:230109/149‑1 (MQ=255) gCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTC‑‑GGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTATCCTTg > 7:142816/1‑149 (MQ=255) cTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTC‑‑GGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTTGAAACAACCGAGCTTTCCTTGGCCg > 7:77066/1‑149 (MQ=255) agcGGCTTCTTCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGTCTAAAACAAAAAAACCCGACGATGTC‑‑GGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGcc < 2:193633/145‑1 (MQ=255) cGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAATCATTAATTGGCTAAAAAAAAAAAACCCGACTATGTCTACGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGcc < 1:111661/147‑1 (MQ=255) tCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTC‑‑GGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAg > 3:286744/1‑149 (MQ=255) cGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTC‑‑GGGTTTTTTTGTGTCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAg < 4:55864/147‑1 (MQ=255) cacCGGCAGAACCTGCCAAGCAGTAATTGGATAAAACAAAAAAACCCGACGATGTC‑‑GGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAGTACACTTtcggtcg < 8:21591/149‑1 (MQ=255) gCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTC‑‑GGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCt < 6:293419/114‑1 (MQ=255) gCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTC‑‑GGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCt > 5:293419/1‑114 (MQ=255) aaGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTC‑‑GGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAGTACACTTTCGGTCGGTGTGAACACCAt < 7:169797/145‑1 (MQ=255) aaGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTC‑‑GGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAGTACACTTTCGGTCGGTGTGAACACCAt > 8:169797/1‑145 (MQ=255) aGTAATTGGCTAAAACAAAAAAACCCGACGATGTC‑‑GGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTTGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAGTACACTTTCGGTCGGTGTGAACACCATCACATCGa < 8:77066/149‑1 (MQ=255) aGTAATTGGCTAAAACAAAAAAACCCGACGATGTC‑‑GGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAGTACACTTTCGGTCGGTGTGAACACCATCACATc > 1:356383/1‑147 (MQ=255) aGTAATTGGCTAAAACAAAAAAACCCGACGATGTC‑‑GGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAGTACACTTTCGGTCGGTGTGAACACCATCACATc < 2:356383/147‑1 (MQ=255) gTAATTGGCTAAAACAAAAAAACCCGACGATGTC‑‑GGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTc > 1:170675/1‑73 (MQ=255) gTAATTGGCTAAAACAAAAAAACCCGACGATGTC‑‑GGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTc < 2:170675/73‑1 (MQ=255) tAAAACAAAAAAACCCGACGATGTC‑‑GGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAAACGAGCTTTCCTTTGCCGGCTCCGACAGTTCACTTTCGGTCGGTGTGAACACCATCACATAGAGTACATACg > 6:165216/1‑148 (MQ=255) aaaaaaaCCCGACGATGTC‑‑GGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAGTACACTTTCGGTCGGTGTGAACACCATCACATCGAGTACATCCGaa < 3:130175/144‑1 (MQ=255) aaaaaaaCCCGACGATGTC‑‑GGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAGTACACTTTCGGTCGGTGTGAACACCATCACATCGAGTACATCCGaa > 4:130175/1‑144 (MQ=255) aaaaaaaCCCGACATCGTC‑‑GGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAGTACACTTTCGGTCGGTGTGAACACCATCACATCGAGTACATCCGAATg < 2:176482/146‑1 (MQ=255) aaaaaaaCCCGACATCGTC‑‑GGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAGTACACTTTCGGTCGGTGTGAACACCATCACATCGAGTACATCCGAATg > 1:176482/1‑146 (MQ=255) cccGACGATGTC‑‑GGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAGTACACTTTCGGTCGGTGTGAACACCATCACATCGAGTACATCCGAATGGAACTCTCGa < 4:154237/149‑1 (MQ=255) ccGACGAAGTC‑‑GGATTTTTTTGTTACTTCTAACTATCGATTAATCGTTTCAGTTGCAAACAACAGAGCAATCGTTGGACGGCTCAGACAGTACACTTTAGGACGGTGTGAACACCATAACATAGATTACAAACCAAAGGAACGCTCTAc > 2:57070/1‑149 (MQ=255) gacgaTGTC‑‑GGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAGTACACTTTCGGTCGGTGTGAACACCATCACATCGAGTACATCCGAATGGAACTCTCGACgg > 8:279325/1‑149 (MQ=255) | AAGCCGCCGAGGAAAACGCCGAAGCTGCCAAAGATCAGGCCGAAGCCGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTC‑‑GGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAGTACACTTTCGGTCGGTGTGAACACCATCACATCGAGTACATCCGAATGGAACTCTCGACGG > NC_002947/4724894‑4725185 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |