Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,732,994 G→T 7.7% Q881K (CAG→AAG)  sucA ← 2‑oxoglutarate dehydrogenase subunit E1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,732,9940GT 7.7% 154.3 / 3.2 52Q881K (CAG→AAG) sucA2‑oxoglutarate dehydrogenase subunit E1
Reads supporting (aligned to +/- strand):  ref base G (27/21);  new base T (2/2);  total (29/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

CGTGCTTCGAAGCGTAACCACAGGCTGGCGCGGCAGAAGCGTCGCGACCGGCGTATTCCAGGACCAGTGCCTTGTTGTGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGATGTCTTCGCGGCCTTCGGCACGGCGTTTTTCCAGCAGGTCGTAGTAGAC  >  NC_002947/4732847‑4733141
                                                                                                                                                   |                                                                                                                                                    
cGTGCTTCGAAGCGTAACCACAGGCTGGCGCGGCAGAAGCGTCGCGACCGGCGTATTCCAGGACCAGTGCCTTGTTGTGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGa                                                                                                                                                     >  6:355343/1‑149 (MQ=255)
     ttCGAAGCGTAACCACAGGCTGGCGCGGCAGAAGCGTCGCGACCGGCGTATTCCAGGACCAGTGCCTTGTTGTGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGacac                                                                                                                                                  >  7:265177/1‑147 (MQ=255)
       cGAAGCGTAACCACAGGCTGGCGCGGCAGAAGCGTCGCGACCGGCGTATTCCAGGACCAGTGCCTTGTTGTGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAg                                                                                                                                               <  5:355343/148‑1 (MQ=255)
            cGTAACCACAGGCTGGCGCGGCAGAAGCGTCGCGACCGGCGTATTCCAGGACCAGTGCCTTGTTGTGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCa                                                                                                                                         >  4:164513/1‑149 (MQ=255)
              tAACCACAGGCTGGCGCGGCAGAAGCGTCGCGACCGGCGTATTCCAGGACCAGTGCCTTGTTGTGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATg                                                                                                                                       >  5:207110/1‑149 (MQ=255)
                  cacaGGCTGGCGCGGCAGAAGCGTCGCGACCGGCGTATTCCAGGACCAGTGCCTTGTTGTGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCATGACCCAAGACCGCATGCtt                                                                                                                                    >  6:14323/1‑148 (MQ=255)
                   acaGGCTGGCGCGGCAGAAGCGTCGCGACCGGCGTATTCCAGGACCAGTGCCTTGTTGTGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGa                                                                                                                                  <  4:86203/149‑1 (MQ=255)
                    caGGCTGGCGCGGCAGAAGCGTCGCGACCGGCGTATTCCAGGACCAGTGCCTTGTTGTGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACCCCAGACCGCATGCTTGAg                                                                                                                                 >  6:297688/1‑149 (MQ=255)
                                      gCGTCGCGACCGGCGTATTCCAGGACCAGTGCCTTGTTGTGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAg                                                                                                              <  3:164513/149‑1 (MQ=255)
                                        gTCGCGACCGGCGTATTCCAGGACCAGTGCCTTGTTGTGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGgt                                                                                                                          <  4:284565/135‑1 (MQ=255)
                                        gTCGCGACCGGCGTATTCCAGGACCAGTGCCTTGTTGTGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGgt                                                                                                                          >  3:284565/1‑135 (MQ=255)
                                         tCGCGACCGGCGTATTCCAGGACCAGTGCCTTGTTGTGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGAt                                                                                                           >  2:18912/1‑149 (MQ=255)
                                             gACCGGCGTATTCCAGGACCAGTGCCTTGTTGTGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTc                                                                                                        >  4:284216/1‑148 (MQ=255)
                                              aCCGGCGTATTCCAGGACCAGTGCCTTGTTGTGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACCCCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGa                                                                                                      >  2:252003/1‑149 (MQ=255)
                                                    gTATTCCAGGACCAGTGCCTTGTTGTGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTAATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGAc                                                                                                     >  7:154951/1‑144 (MQ=255)
                                                         ccAGGACCAGTGCCTTGTTGTGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTTACACCA‑ACCTCAAGCATGCGGTCTGGTGTCAggaag                                                                                                                 <  3:228866/127‑4 (MQ=25)
                                                         ccAGGACCAGTGCCTTGTTGTGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTTACACCA‑ACCTCAAGCATGCGGTCTGGTGTCAggaag                                                                                                                 >  4:228866/1‑124 (MQ=25)
                                                         ccAGGACCAGTGCCTTGTTGTGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTc                                                                                            <  1:18912/148‑1 (MQ=255)
                                                         ccAGGACCAGTGCCTTGTTGTGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTc                                                                                            <  4:244383/148‑1 (MQ=255)
                                                                cAGTGCCTTGTTGTGGCGGCCCAGGATACGGCGCATGTGGAGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCAGACACCAGACCGCATGCATGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAggg                                                                                     >  6:87352/1‑148 (MQ=255)
                                                                  gTGCCTTGTTGTGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAAc                                                                                  <  3:284216/149‑1 (MQ=255)
                                                                         tttgtGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATAc                                                                            <  8:154951/147‑1 (MQ=255)
                                                                         gttgtGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGa                                                                               >  2:355093/1‑145 (MQ=255)
                                                                         gttgtGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGa                                                                               <  1:355093/145‑1 (MQ=255)
                                                                         gttgtGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACa                                                                           >  4:208198/1‑149 (MQ=255)
                                                                              ggcggcCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAgctgc                                                                      <  8:265177/149‑1 (MQ=255)
                                                                                         aTACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAg                                                           >  6:342503/1‑149 (MQ=255)
                                                                                                gcATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGc                                                     >  1:227058/1‑148 (MQ=255)
                                                                                                gcATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCg                                                    <  6:207110/149‑1 (MQ=255)
                                                                                                  aTGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGAt                                                  <  3:208198/149‑1 (MQ=255)
                                                                                                       gtgCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGATGTCtt                                             >  4:209375/1‑149 (MQ=255)
                                                                                                          ctggctgCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGATGTCTTc                                            >  2:128071/1‑147 (MQ=255)
                                                                                                          ctggctgCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGATGTCTTc                                            <  1:128071/147‑1 (MQ=255)
                                                                                                           tggctgCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTATGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGATGTCTTcgcg                                         >  6:66655/1‑149 (MQ=255)
                                                                                                                  tgTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGATGTCTTCGCGGCCTTCg                                  >  8:143692/1‑149 (MQ=255)
                                                                                                                   gTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCg                                                                                                       >  5:4830/1‑79 (MQ=255)
                                                                                                                   gTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCg                                                                                                       <  6:4830/79‑1 (MQ=255)
                                                                                                                    tACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGATGTCTTCGCGGCCTTCgg                                 >  8:204554/1‑148 (MQ=255)
                                                                                                                      ccAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGATGTCTTCGCGGCCTTCGGCAc                              >  8:312781/1‑149 (MQ=255)
                                                                                                                      ccAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGATGTCTTCGCGGCCTTCGGCAc                              >  2:66076/1‑149 (MQ=255)
                                                                                                                            gcCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGATGTCTTCGCGGCCTTCGGCACGGCGtt                        <  7:196244/149‑1 (MQ=255)
                                                                                                                                  gTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCTAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGATGTCTTCGCGGCCTTCGGCACGGCGTTTTTc                    <  5:297688/147‑1 (MQ=255)
                                                                                                                                  gTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGATGTCTTCGCGGCCTTCGGCACGGCGTTTTTCca                  <  5:342503/149‑1 (MQ=255)
                                                                                                                                      aTCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGATGTCTTcgcg                                         <  6:114584/122‑1 (MQ=255)
                                                                                                                                      aTCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGATGTCTTcgcg                                         >  5:114584/1‑122 (MQ=255)
                                                                                                                                            tctTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGATGTCTTCGCGGCCTTCGGCACGGCGTTTTTCCAGCAGGTCgta        >  7:133043/1‑149 (MQ=255)
                                                                                                                                              ttCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCTGGATGGCGATGTCTTCGCGGCCTTCGGCACGGCGTTTTTCCAGCAGGTCgta        <  5:87352/147‑1 (MQ=255)
                                                                                                                                               cccTTACACCA‑ACCTCAAGCATGCGGTCTGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGAt                                                  <  6:317791/103‑1 (MQ=25)
                                                                                                                                               cccTTACACCA‑ACCTCAAGCATGCGGTCTGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGAt                                                  >  5:317791/2‑104 (MQ=25)
                                                                                                                                                ccTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGATGTCTTCGCGGCCTTCGGCACGGCGTTTTTCCAGCAGGTCgtagta     <  7:312781/148‑1 (MQ=255)
                                                                                                                                                 cTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGATGTCTTCGCGGCCTTCGGCACGGCGTTTTTCCAGCAGGTCGTAGTAGa   <  3:209375/149‑1 (MQ=255)
                                                                                                                                                  tGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGATGTCTTCGCGGCCTTCGGCACGGCGTTTTTCCAGCAGGTCGTAGTAGAc  >  4:239603/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CGTGCTTCGAAGCGTAACCACAGGCTGGCGCGGCAGAAGCGTCGCGACCGGCGTATTCCAGGACCAGTGCCTTGTTGTGGCGGCCCAGGATACGGCGCATGTGGTGCTGGCTGCTGTACCAGGCGCCCTGGTTCATCGGCTCTTCCTGACACCAGACCGCATGCTTGAGGT‑TGGTGTAAGGGGCGAGGATTTCGACCAGATCGTCCTCAGGGAACGGATACAGCTGCTCGATACGCAGGATGGCGATGTCTTCGCGGCCTTCGGCACGGCGTTTTTCCAGCAGGTCGTAGTAGAC  >  NC_002947/4732847‑4733141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑