Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,740,844 C→G 13.4% intergenic (+81/+672) gltA → / ← yeiW citrate synthase/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,740,8440CG13.4% 121.8 / 11.5 52intergenic (+81/+672)gltA/yeiWcitrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (24/20);  new base G (3/4);  total (28/24)
Fisher's exact test for biased strand distribution p-value = 6.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.09e-01

CGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACAC‑CACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTG‑AACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCGGTGTGCACCTG  >  NC_002947/4740698‑4740991
                                                                                                                                                  |                                                                                                                                                     
cGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCcg                                                                                                                                                    >  3:90952/1‑149 (MQ=255)
    cgcgCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGaa                                                                                                                                                <  3:180302/149‑1 (MQ=255)
    cgcgCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGa                                                                                                                                                 <  1:91866/148‑1 (MQ=255)
      cgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAaga                                                                                                                                              <  5:155687/149‑1 (MQ=255)
       gcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAagag                                                                                                                                             <  3:18973/149‑1 (MQ=38)
          aGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGcc                                                                                                                                          <  4:90952/149‑1 (MQ=255)
                  acCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAagag                                                                                                                                             <  6:202456/138‑1 (MQ=255)
                  acCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAagag                                                                                                                                             >  5:202456/1‑138 (MQ=255)
                  acCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCACGCGAAGAGGCCGGCACAgc                                                                                                                                  >  8:80706/1‑148 (MQ=255)
                            aGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAaga                                                                                                                                              >  8:153787/1‑127 (MQ=255)
                            aGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAaga                                                                                                                                              <  7:153787/127‑1 (MQ=255)
                                 cgcgACAGCGTTGCCCTTAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGACGGCTGCCCTCGGGCAGCC‑TTTTTAATTTGACCTGTCCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACAC‑CACAAAACCTCa                                                                                                                  <  3:226276/149‑1 (MQ=255)
                                                 tGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAaga                                                                                                                                              <  3:163833/106‑1 (MQ=25)
                                                 tGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAaga                                                                                                                                              >  4:163833/1‑106 (MQ=25)
                                                   aaaGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACCC‑CACAAAACCTCAGTTTTTATACGCCCGCt                                                                                                 >  8:301340/1‑148 (MQ=255)
                                                      caCCGAAAGTAAGCGGCAGTAGCCTAACGAAAGAAGGCTGCCCTCGGGCAG‑CTTTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGTGGCCGGCACAGACAC‑CACAAAACCTCAGTTTTTCTCCGCCCGCTCACg                                                                                             <  8:116639/148‑1 (MQ=255)
                                                              gTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGACCGCGAAGAGGCCGGCACAGACAC‑CACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCtt                                                                                     >  2:54581/1‑149 (MQ=255)
                                                               tAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACAC‑CACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCttt                                                                                    >  7:198557/1‑149 (MQ=255)
                                                                   cTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGCGGCCGGCACAGACAC‑CCCAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAgg                                                                                >  5:352618/1‑149 (MQ=255)
                                                                   cTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACAC‑CACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAgg                                                                                <  8:198557/149‑1 (MQ=255)
                                                                      cAGTAGCCTACCGCATCAAGGCGGCCCTCGGGCAG‑CTTTTTTTATTTGTCGTGTGCCGGCCTCTTCGCGGCCTTGCCCGCTAAGAGGCCGGCACAGACAC‑CACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTa                                                                             <  4:350610/149‑1 (MQ=255)
                                                                                    aagaagGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGCAGAGGCCGGCACAGCCCC‑CACAAAACCTCAGGTTTTCTCCGCCCGCTCCCGCAGCCCTTTCAGGGGATTG‑CACGGCGCATc                                                              >  7:56001/1‑149 (MQ=255)
                                                                                      gaagGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACAC‑CACAAAACCTCAGTTTTTCTACGCCCGCACCCGCAGCCCTTTCAGGGTATTG‑AACGGCGCCTcca                                                            >  4:255224/1‑149 (MQ=255)
                                                                                            tGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCCAAGACAC‑CACAAAACCTCAGTTTTTCTCAGCCCGCTAACGCAGCCCTTTAAGGGTATTG‑AACGGCGCATCCACCACg                                                       >  7:249603/1‑148 (MQ=255)
                                                                                            tGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACAC‑CACAAAACCTCACTTTTTATCCCCCCGCTCACGCAGCCCTTTCAGGGTATTG‑AACGGCGCATCCACCACg                                                       >  1:198702/1‑148 (MQ=255)
                                                                                              cccTCGGTCAGGC‑TTTTTTATTTGCCCTGTGCCGGCCTCTTCGCGGCCTTGCCAGCGAAGAGGCCGGCACAGACAC‑CACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTG‑AACGGCGCATCCACCACGAAc                                                    <  8:241129/149‑1 (MQ=255)
                                                                                                cTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCCGCCATGCCCGCGAAGAGGCCGGCACAAAAAC‑CACAAAACCACACATTTTCTCCGCCCGCACCCGCAGCCCATTAAGGGTATTG‑AACCGCGCATCCACCACGAACta                                                  >  2:285735/1‑148 (MQ=255)
                                                                                                 tCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACAC‑CACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTG‑AACGGCGCATCCACCACGAACttg                                                 >  8:149691/1‑149 (MQ=255)
                                                                                                 tCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCATCGCGGCCTTTCCCGCGAAGAGGCCGGCACAGAAAC‑CACAAAACAACAGTTTTTATACCCCCCCTCACGCACCCCGTTCAGTGTATTT‑AACGGCGCATc                                                              >  8:221383/1‑136 (MQ=255)
                                                                                                 tCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTATGCGCGGCCTTGCCCGCTAATAGGCCGGCACAGACAC‑CACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTG‑AACGGCGCATc                                                              <  7:221383/136‑1 (MQ=255)
                                                                                                     gcctgcattttttATTTGCCCTGTGCGGGCCTATTCGCGGCCTTGCCCGCTAAGAGGCCGGCACAGACAC‑CACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTG‑AACGGCGCATCCACCACGAACttgttg                                              <  7:301340/142‑1 (MQ=255)
                                                                                                     gCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCACTTCGCGGACATGCCCCCGAAGCGGACGGCACACCCAC‑CACAAAACCACACTTTTTCTCCCCCCGCTAACGCAGCCCTTTCAGGGTAATT‑AACGGCGCATCCACCACGAACTTGATgg                                             >  2:100556/1‑149 (MQ=255)
                                                                                                          ccttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACAC‑CACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAggg                                                                               <  8:267592/110‑1 (MQ=255)
                                                                                                          ccttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACAC‑CACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAggg                                                                               >  7:267592/3‑112 (MQ=255)
                                                                                                            ttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACAC‑CACAAAACCTCAGTTTTTCTcc                                                                                                        <  6:177011/85‑1 (MQ=38)
                                                                                                            ttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACAC‑CACAAAACCTCAGTTTTTCTcc                                                                                                        >  5:177011/1‑85 (MQ=38)
                                                                                                            ttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACAC‑CACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTG‑AACGGCGCATCCACCACGAACTTGTTGGccacca                                       >  5:186011/1‑149 (MQ=255)
                                                                                                                 tATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACAC‑CACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTG‑AACGGCGCATCCACCACGAACTTGTTGGCCACCATCGc                                   <  6:186011/148‑1 (MQ=255)
                                                                                                                         ctgTGCCGGCCTCTTAGCGGCCTTTCCCCCGAAGAGGCCGGCCCAGACAC‑CACAAAAACACAGTTTTTATACGCCCGCTCACGCAGCCCGTTAAGGGTATTT‑AAAGGCGCATCCACCACGAACTTGTTGGCCACCATAGCCGGCCCGc                           >  4:350583/3‑148 (MQ=255)
                                                                                                                              ccGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACAC‑CACAAAACCTCCGTTTGTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTG‑AACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTaccac                     >  5:254081/1‑149 (MQ=255)
                                                                                                                                 gCCTCTTCGCGGGCAAGGCCGCGAAGAGGCCGGCACAGACAC‑CACAAAACCTCAGTTTTTCTCAGCCCGCTCACGCAGCCCTTTCAGGGTATTG‑ACCGGCGCATCCACCACGAACTTGTTTGCCACCATCGCCGGCACGCTACCACCTg                  <  7:179529/149‑1 (MQ=39)
                                                                                                                                    tctTCGCGGGCAAGGCCGCGAAGAGGCCGGCACAGACAC‑CACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTG‑AACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCt               >  7:8529/1‑149 (MQ=255)
                                                                                                                                    tctTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACAC‑CACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTG‑AACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCt               >  6:271917/1‑149 (MQ=255)
                                                                                                                                     ctTCGCGGGCAAGGCCGCGAAGAGGCCGGCACAGACAC‑CACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTG‑AACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTgg                 >  7:183251/1‑146 (MQ=255)
                                                                                                                                     ctTCGCGGGCAAGGCCGCGAAGAGGCCGGCACAGACAC‑CACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTG‑AACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTgg                 <  8:183251/146‑1 (MQ=255)
                                                                                                                                      ttCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACAC‑CACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTG‑AACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCg             >  4:196786/1‑149 (MQ=255)
                                                                                                                                         gcgGCCTTGCCCGCGAAGAGGCCGGCACAGACAC‑CACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTG‑AACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCGgtg          <  1:54581/149‑1 (MQ=255)
                                                                                                                                          cgGCCTTGCCCGCGAAGAGGCCGGCACAGACAC‑CACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCGgtg          <  1:285735/149‑1 (MQ=255)
                                                                                                                                            gCCTTGCCCGCGAAGAGGCCGGCACAGACAC‑CACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTG‑AACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCGGTGTGc       <  3:196786/149‑1 (MQ=255)
                                                                                                                                             ccTTGCCCGCGAAGAGGCCGGCACAGACACGCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTG‑AACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCGGtgtg        >  1:31862/1‑148 (MQ=255)
                                                                                                                                                 gCCCGCGAAGAGGCCGGCACAGACAC‑CACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTG‑AACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCGGTGTGCACCt   <  7:80706/148‑1 (MQ=255)
                                                                                                                                                 gCCCGCGAAGAGGCCGGCACAGACAC‑CACAAAACCTCAGTTTATCTCCGACCGCTCACGCAGCCCTATCAGGCTATTG‑AACGGCGCATCCACCACGAACATGTTGGACACCATCGCCCGCACGCTACCACCTGGCTCGGTGTGCACCTg  >  4:306038/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
CGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACAC‑CACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTG‑AACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCGGTGTGCACCTG  >  NC_002947/4740698‑4740991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑