| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 358,091 | G→A | 11.6% | intergenic (+59/‑71) | tdcG‑I → / → PP_5441 | L‑serine dehydratase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 358,091 | 0 | G | A | 11.6% | 78.8 / 2.0 | 36 | intergenic (+59/‑71) | tdcG‑I/PP_5441 | L‑serine dehydratase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (19/12); new base A (2/2); total (21/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.93e-01 | |||||||||||
CATGCGCGATACCGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGG‑CCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTC‑TTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAGTGTGGGCGTTGTCGG > NC_002947/357948‑358237 | cATGCGCGATACCGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATc > 5:355120/1‑149 (MQ=255) ccGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGGATTGCACAGTTCCTGTAGGAg < 8:84108/148‑1 (MQ=37) cGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTATTCGCGGGCAAGCCCGCTCCTAAACGAACAGTGGAATCCCTGTAGGAGCg > 2:279441/1‑149 (MQ=255) ggCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGGATTGCACAGTTCCTGTAGGAGCgg < 4:331585/149‑1 (MQ=37) aaGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCCCGGGCAAGCCCCCACCTACAGGAACAGTGCCAAACCTGTAGGAGCCGGCTTGCCCGCGAACAGG‑c > 2:42723/1‑149 (MQ=255) gTACAAAGAGACCTCGCGCGGCGCCCTGGCCGTCACCGCTATCGAGAGCATATTTTCTGACCTGACCTTCTGCGCCTCTTCGCGGGCAAGCCCCATACTTCACGAACAGTACATTACCAGTACGAGCCGGCTTTCCCCCCAAGCGG‑ccc > 6:358143/1‑149 (MQ=255) tACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGG‑ccca > 1:144488/1‑148 (MQ=255) aaaGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCGACAGGGACTGTGGAATGCCGGTAGGCGCGGGCGTGGCCGCGGAGAGG‑CCCGTgg > 1:196953/1‑148 (MQ=37) aaaGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCGACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGG‑CCCtt > 6:168882/1‑147 (MQ=255) cTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGG‑CCCTTGAGGCTGATg > 7:113998/1‑149 (MQ=255) tCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTTCAGGAACGGTGCAATACCGGTAGGAGCGGGCTTGCCCGCGCAGCGG‑CCCGTGAGGCTGATg > 7:19069/1‑148 (MQ=38) cggcggCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGG‑CCCTTGAGGCTGATGCAGGTg < 6:355120/149‑1 (MQ=255) ggcggggcggcCCGCGCTATCCTGTGGTTATTTTCTGCCCTCCCTTGCTGGGCCTCTTCGCGGCAACCCCCGCCCCTCCCGAAACTGTCCAACCCGTGTCGGCGGGGGCTGCCCCGCGAAGAGG‑CCCTTGAGGCTGATGCAGGTGCAac < 2:196953/144‑1 (MQ=255) gggccgcgcTATCGAGTGCTGTTTTTCTTAGCGGACCTTCTGGGCCTCTTCGCGGCCAAGCCGGCTCCTACCGCAACTGTGCAATCCCTGTAGGAGGGGGCTTGCCCGCGAAGAGG‑CCCTTGAGGCTGATGCAGGTGCAACACAACCGc < 8:19069/144‑1 (MQ=37) aGCGCTATCGTGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTa < 1:42723/149‑1 (MQ=255) aGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGG‑CCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTAt < 2:116255/149‑1 (MQ=255) cTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGG‑CCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTC‑TTTATGGTg > 5:170378/1‑149 (MQ=255) tGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGG‑CCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTC‑TTTATGGTGc > 5:338101/1‑149 (MQ=255) tGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCAACAGGAACAGTGCAATCCCTGGAGGAGCGGGCTTGCCCGCGAAGAGG‑CCCTTGAGGCTGATGCAGGTGAAACACAACCGCCTATGCCGC‑TTTAAGGTGc > 5:326239/1‑149 (MQ=38) tttttttGAGCTCACCTGCTGGGCTGTTTCGCGGCAATGCCCGGTCATACTGCAATGTTGCAAACCATGTTGGAGCGGGCTTGCCCGCGAAGAGG‑CCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTC‑TTTATGGTGCg < 1:279441/147‑1 (MQ=255) ttttCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGG‑CCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTC‑TTTATGGTGCgg < 6:338101/148‑1 (MQ=255) cTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACTGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGG‑CCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTC‑TTTATGGTGCGGTGATg > 1:254741/1‑149 (MQ=255) cTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGG‑CCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTC‑TTTATGGTGCGGTGAt < 6:170378/148‑1 (MQ=255) cTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTCGGAGCGGGCTTGCCCGCGAAGAGG‑CCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTC‑TTTATGGTGCGGTGATGGGCGCCCCg < 7:36522/145‑1 (MQ=255) cTGGGCCTCTTCGCGGGCAAAACCGCTCCCACAGGAACAGTGGAATCCCGGTTAGAACCCGCATGGCCCCCAAGACG‑CCCGTGGCGCGGATGCACCTGCAACCAAACCGCCAAAGGCTCGTTTAT‑GTTAGGGGATGGGCGgcccc > 8:36522/1‑144 (MQ=255) tctTCGCGGGCAAGCCCGCAACGACAGGAAAAGTGCAATACCAGGAGGAGCGGGGTTGGCCGCGAAGAGG‑CCCTTGAGGCTGATGCAAGGGAAACACAACCGCCAATTCCTA‑TTTATGGTGGGGTGATGGGCGCCCCGCCAACCGCtcg > 2:278785/1‑149 (MQ=255) ttCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCGGTAGGAGCGGGCTTGCCCGCGAAGAGG‑CCCGTGAGGCTGCTGCAGGTGCAACACACCCGCCTATGCCTC‑GTTATGGTGCGGGGCTGGGCGCCCCGCCTACCGAacgtc > 3:296624/1‑149 (MQ=37) cgcgGGCAAGCCCGCTCCTACAGGACCTGTGCAAGCCCTGTCGTAGCGGGCTTGCCCGCGAAGAGG‑CCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTC‑TTTATGGTGCGGTGATGGGCGCCCCGCCTACCGAtcgt < 1:232059/146‑1 (MQ=255) gcgGGCAAGCCCGCTACTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGG‑CCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTC‑TTTATGGTGCGGTGATGGGCGCCCCGCCTACCGCTCGTCGGa > 4:140029/1‑149 (MQ=255) gCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGG‑CCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTC‑TTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTc > 5:28433/1‑149 (MQ=255) gCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGG‑CCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTC‑TTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTc > 7:273856/1‑149 (MQ=255) gCTCCTACAGGGATTGCACAGTTCCTGTAGGAGCGGGCTTGCCCGCGAAGAGG‑CCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTC‑TTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAg > 7:345105/1‑149 (MQ=37) tCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGG‑CCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTC‑TTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAgtg > 1:95734/1‑149 (MQ=255) cTACTGGAACTGGGCAATCCCGGTCGGCGGGGGCTTGCCCGCGAAGAGG‑CCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTC‑TTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAGtgtg < 6:326239/149‑1 (MQ=255) catcTGAGCATTCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGG‑CCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTC‑TTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAGTGTGGGCGtt < 5:358143/146‑1 (MQ=255) aaCTGTGCAATCCCTGTTGGTGCGGGCTTGCCCGCGAAGAGG‑CCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTC‑TTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAGTGTGGGCGTTg < 4:296624/149‑1 (MQ=255) tgCAATCCGTGTAGGAGCGGGCTTGCCCGCGAAGAGG‑CCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTC‑TTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAGTGTGGGCGTTGTCgg < 8:113998/148‑1 (MQ=255) | CATGCGCGATACCGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGG‑CCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTC‑TTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAGTGTGGGCGTTGTCGG > NC_002947/357948‑358237 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |