| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,783,691 | C→T | 8.7% | R1208Q (CGG→CAG) | pvdJ ← | non‑ribosomal peptide synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,783,691 | 0 | C | T | 8.7% | 160.5 / 4.8 | 57 | R1208Q (CGG→CAG) | pvdJ | non‑ribosomal peptide synthetase |
| Reads supporting (aligned to +/- strand): ref base C (31/21); new base T (3/2); total (34/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.61e-01 | |||||||||||
TCGCGCCCATTGGCGCAGGGGTGCCGACCTGGACGGCAGGCTCACCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAG‑GCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTCCAGAGTGACTACCTGCAGGAGGGCCTGCAC > NC_002947/4783543‑4783833 | tCGCGCCCATTGGCGCAGGGGTGCCGACCTGGACGGCAGGCTCACCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACTGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGcc < 5:361912/149‑1 (MQ=255)tCGCGCCCATTGGCGCAGGGGTGCCGACCTGGACGGCAGGCTCACCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGcc < 8:31156/149‑1 (MQ=255) gcgcCCATTGGCGCAGGGGTGCCGACCTGGACGGCAGGCTCACCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGt > 3:294093/1‑149 (MQ=255) ccATTGGCGCAGGGGTGCCGACCTGGACGGCAGGCTCACCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACGGCTGTTGCt > 7:211609/1‑149 (MQ=255) aTTGGCGCAGGGGTGCCGACCTGGACGGCAGGCTCACCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGa < 8:144068/149‑1 (MQ=255) ggCGCAGGGGTGCCGACCTGGACGGCAGGCTCACCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACGGCTGTTGCTggcg < 8:211609/148‑4 (MQ=255) cTGGACGGCAGGCTCACCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACaa > 4:239395/1‑149 (MQ=255) gACGGCAGGCTCACCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAAt < 5:135715/147‑1 (MQ=255) aCGGCAGGCTCACCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTc < 8:232555/149‑1 (MQ=255) ggCAGGCTCACCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGc > 6:168273/1‑149 (MQ=255) aCCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGc > 2:153836/1‑111 (MQ=255) cGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGGCACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCt > 5:302030/1‑149 (MQ=255) ccTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCg > 2:267180/1‑149 (MQ=255) cccTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGaa > 6:204914/1‑138 (MQ=255) cccTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGaa < 5:204914/138‑1 (MQ=255) cccTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCAc < 4:360111/149‑1 (MQ=255) ggCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGCATGAGACAAGACACCCCATCCACCACCAGGTGATGCACCTACAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACATTGTCGCCCTGAACAGCTCGCCACGGGcc < 6:335544/149‑1 (MQ=255) gCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGc > 3:259458/1‑147 (MQ=255) gCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGc > 3:198728/1‑147 (MQ=255) tgctgGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAg > 1:51886/1‑149 (MQ=255) gTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGaa > 8:277/1‑105 (MQ=255) tAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAG‑GCTGGc > 3:40211/1‑149 (MQ=255) ggCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAG‑GCTGGcc < 2:267371/148‑1 (MQ=255) ccGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCCGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAG‑GCTGGCCTg > 3:353754/1‑148 (MQ=255) gTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAG‑GCTGGCCTGCACCTCGTTgg < 4:259458/149‑1 (MQ=255) cTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAG‑GCTGGCCTGCACCTCGTTggcg > 2:138311/1‑149 (MQ=255) ttCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAG‑GCTGGCCTGCACCTCGTTggcgg > 7:246793/1‑149 (MQ=255) cGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAG‑GCTGGCCTGCACCTCGTTGgcggcg > 4:101596/1‑149 (MQ=255) aaCAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAG‑GCTGGCCTGCACCTCGTTGGCGGCGGt < 5:168273/149‑1 (MQ=255) gATGCGCCAAGGCACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAG‑GCTGGCCTGCACCTCGTTGGCGGCGGTTTCTa < 6:302030/149‑1 (MQ=255) tGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAG‑GCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAgc > 4:34523/1‑149 (MQ=255) cAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAG‑GCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGc < 3:101596/149‑1 (MQ=255) acacCCCATCCACCACCAGGTGATGCACCGCCACCAACAGCCGTTGCTGCCCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAG‑GCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAgg > 1:130489/1‑149 (MQ=255) acCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGc < 5:188593/88‑1 (MQ=255) acCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGc > 6:188593/1‑88 (MQ=255) acCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAG‑GCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCt > 6:166710/1‑149 (MQ=255) cccATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAG‑GCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGt > 6:229751/1‑149 (MQ=255) ccATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAG‑GCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTc > 7:22248/1‑149 (MQ=255) cATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAGTGCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTc < 1:267180/149‑1 (MQ=255) aTCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAG‑GCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTCCa < 5:229751/149‑1 (MQ=255) aTCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCCGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAG‑GCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTCCa < 4:353754/149‑1 (MQ=255) caccaccaGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAG‑GCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTCCaga < 8:246793/148‑1 (MQ=255) accaccaGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAG‑GCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTCCAGAGt < 4:294093/149‑1 (MQ=255) accaccaGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAG‑GCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTCCAGAGt > 4:276162/1‑149 (MQ=255) caccaGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAG‑GCTGGCCTGCACCTCGTTggcgg > 1:152642/1‑116 (MQ=255) caccaGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAG‑GCTGGCCTGCACCTCGTTggcgg < 2:152642/116‑1 (MQ=255) caccaGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAG‑GCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTCCAGAGTGa > 8:124107/1‑149 (MQ=255) accaGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAG‑GCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTCCAGAGTGAc > 6:334858/1‑149 (MQ=255) accaGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAG‑GCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTCCAGAGTGAc < 5:334858/149‑1 (MQ=255) gCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAG‑GCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTCCaga > 6:184130/1‑134 (MQ=255) gCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAG‑GCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTCCaga < 5:184130/134‑1 (MQ=255) cGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAG‑GCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTCCAGAGTGACTACCTGCAGGAggg > 6:34320/1‑149 (MQ=255) cGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAG‑GCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTCCAGAGTGACTACCTGCAGGAggg > 1:201345/1‑149 (MQ=255) gtcAGCAACGGCTGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAG‑GCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTCCAGAGTGACTACCTg < 7:32718/138‑1 (MQ=255) gtcAGCAACGGCTGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAG‑GCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTCCAGAGTGACTACCTg > 8:32718/3‑140 (MQ=255) tcAGCAACGGCTGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAG‑GCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTCCAGAGTGACTACCTGCAGGAGGGcc > 4:103451/2‑149 (MQ=255) ccAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAG‑GCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTCCAGAGTGACTACCTGCAGGAGGGcc < 4:198728/149‑1 (MQ=255) cAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAG‑GCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTCCAGAGTGACTACCTGCAGGAGGGCCTGCa > 4:94139/1‑149 (MQ=255) aaCAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAG‑GCTGGCCTGCACCTCGTTGGGGGCGGTTTCTAGCGCTGCAAGGCTGTCCAGAGTGACTACCTGCAGGAGGGCCTGCAc > 5:116276/1‑149 (MQ=255) | TCGCGCCCATTGGCGCAGGGGTGCCGACCTGGACGGCAGGCTCACCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAG‑GCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTCCAGAGTGACTACCTGCAGGAGGGCCTGCAC > NC_002947/4783543‑4783833 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |