Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,795,645 A→C 7.7% R266R (CGT→CGG)  pvdH ← diaminobutyrate‑2‑oxoglutarate transaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,795,6450AC 7.7% 147.7 / 2.3 49R266R (CGT→CGG) pvdHdiaminobutyrate‑2‑oxoglutarate transaminase
Reads supporting (aligned to +/- strand):  ref base A (24/21);  new base C (2/2);  total (26/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

GTACCGGCATGCGCACCTGGCTTCCAGGTGTCCAGCCAGTCGCGATAGACCACTACCGCCAGCGGCAGGCTGCCACCGATGGCTTTGGACAAGGTGACCACATCAGGCACGATACCGGCGTGCTCGAAGG‑CAAACATGCGCCCGGTACGGGCGAAGCCGCTCTGGATCTCGTCGACGATCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGC  >  NC_002947/4795499‑4795792
                                                                                                                                                   |                                                                                                                                                   
gTACCGGCATGCGCACCTGGCTTCCAGGTGTCCAGCCAGTCGCGATAGACCACTACCGCCAGCGGCAGGCTGCCACCGATGGCTTTGGACAAGGTGACCACATCAGGCACGATACCGGCGTGCTCGAAGG‑CAAACATGCGCCCGGTACg                                                                                                                                                   >  4:263641/1‑149 (MQ=255)
   ccGGCATGCGCACCTGGCTTCCAGGTGTCCAGCCAGTCGCGATAGACCACTACCGCCAGCGGCAGGCTGCCACCGATGGCTTTGGACAAGGTGACCACATCAGGCACGATACCGGCGTGCTCGAAGG‑CAAACATGCGCCCGGTACggg                                                                                                                                                 <  1:279454/148‑1 (MQ=255)
      gCATGCGCACCTGGCTTCCAGGTGTCCAGCCAGTCGCGATAGACCACTACCGCCAGCGGCAGGCTGCCACCGATGGCTTTGGACAAGGTGACCACATCAGGCACGATACCGGCGTGCTCGAAGG‑CAAACATGCGCCCGTACCGGGCgca                                                                                                                                             <  8:40710/149‑3 (MQ=255)
      gCATGCGCACCTGGCTTCCAGGTGTCCAGCCAGTCGCGATAGACCACTACCGCCAGCGGCAGGCTGCCACCGATGGCTTTGGACAAGGTGACCACATCAGGCACGATACCGGCGTGCTCGAAGG‑CAAACATGCGCCCGGTACGGGCGaa                                                                                                                                             <  7:208052/149‑1 (MQ=255)
         tGCGCACCTGGCTTCCAGGTGTCCAGACAGTCGCGATAGACCACTACCGCCAGCGGCAGGCTGCCAACGATGGCTTTGGACAAGGTGACCACATCAGGCACGATACCGGCGTGCTCGAAGG‑CAAACATGCGCCCGGTACGGGCGAAGcc                                                                                                                                          >  7:185027/1‑149 (MQ=255)
          gcgcACCTGGCTTCCAGGTGTCCAGCCAGTCGCGATAGACCACTACCGCCAGCGGCAGGCTGCCACCGATGGCTTTGGACAAGGTGACCACATCAGGCACGATACCGGCGTGCTCGAAGG‑CAAACATGCGCCCGGTACGGGCGAAGCCg                                                                                                                                         >  5:129623/1‑149 (MQ=255)
             cACCTGGCTTCCAGGTGTACAGCCAGTCGCGATAGACCACTACCGCGATCGGCAGGCTGCCACCGATGGCTTTGTACAAGGTGACCACATCAGGCACGATACCGGCGTGCTCGAAGG‑CAAACATGCGCCCGGTACGGGCGAAGCCGctc                                                                                                                                      <  8:185027/149‑1 (MQ=255)
                     ttCCAGGTGTCCAGCCAGTCGCGATAGACCACTACCGCCAGCGGCAGGCTGCCACCGATGGCTTTGGACAAGGTGACCACATCAGGCACGATACCGGCGTGCTCGAAGG‑CAAACATGCGCCCGGTACGGGCGAAGCCGCTCTGGAtctc                                                                                                                              >  5:244076/1‑149 (MQ=255)
                        cAGGTGTCCAGCCAGTCGCGATAGACCACTACCGCCAGCGGCAGGCTGCCACCGATGGCTTTGGACAAGGTGACCACATCAGGCACGATACCGGCGTGCTCGAAGG‑CAAACATGCGCCCGGTACGGGCGAAGCCGCTCTGGATCtcgtc                                                                                                                           >  8:46500/1‑149 (MQ=255)
                         aGGTGTCCAGCCAGTCGCGATAGACCACTACCGCCAGCGGCAGGCTGCCACCGATGGCTTTGGACAAGGTGACCACATCAGGCACGATACCGGCGTGCTCGAAGG‑CAAACATGCGCCCGGTACGGGCGAAGCCGCTCTGGATCtcgtc                                                                                                                           >  5:263328/1‑148 (MQ=255)
                              tCCAGCCAGTCGCGATAGACCACTACCGCCAGCGGCAGGCTGCCACCGATGGCTTTGGACAAGGTGACCACATCAGGCACGATACCGGCGTGCTCGAAGG‑CAAACATGCGCCCGGTCCGGGCGAAGCCGCTCTGGATCTCGTcgacga                                                                                                                      >  4:246388/1‑148 (MQ=255)
                              tCCAGCCAGTCGCGATAGACCACTACCGCCAGCGGCAGGCTGCCACCGATGGCTTTGGACAAGGTGACCACATCAGGCACGATACCGGCGTGCTCGAAGG‑CAAACATGCGCCCGGTACGGGCGAAGc                                                                                                                                           >  6:230907/1‑127 (MQ=255)
                              tCCAGCCAGTCGCGATAGACCACTACCGCCAGCGGCAGGCTGCCACCGATGGCTTTGGACAAGGTGACCACATCAGGCACGATACCGGCGTGCTCGAAGG‑CAAACATGCGCCCGGTACGGGCGAAGc                                                                                                                                           <  5:230907/127‑1 (MQ=255)
                               gaagccagTCGCGATAGACCACTACCGCCAGCGGCAGGCTGCCACCGATGGCTTTGGACAAGGTGACCACATCAGGCACGATACCGGCGTGCTCGAAGG‑CAAACATGCGCCCGGTACGGGCGAAGCCGCTCTGGATCTCGTCGACGATc                                                                                                                    <  1:100672/147‑1 (MQ=255)
                                    cagTCGCGATAGACCACTACCGCCAGCGGCAGGCTGCCACCGATGGCTTTGGACAAGGTGACCACATCAGGCACGATACCGGCGTGCTCGAAGG‑CAAACATGCGCCCGGTACGGGCGAAGCCGCTCTGGATCTCGTCGACGATCAacgc                                                                                                               >  4:99967/1‑149 (MQ=255)
                                          cgATAGACCACTACCGCCAGCGGCAGGCTGCCACCGATGGCTTTGGACAAGGTGACCACATCAGGCACGATACCGGCGTGCTCGAAGG‑CAAACATGCGCCCGGTACGGGCGAAGCCGCTCTGGATCTCGTCGACGATCAacgccacgcc                                                                                                         >  4:187838/1‑149 (MQ=255)
                                             tAGACCACTACCGCCAGCGGCAGGCTGCCACCGATGGCTTTGGACAAGGTGACCACATCAGGCACGATACCGGCGTGCTCGAAGG‑CAAACATGCGCCCGGTACGGGCGAAGCCGCTCTGGATCTCGTCGACGATCAACGCCAcgcccgc                                                                                                      <  7:46500/149‑1 (MQ=255)
                                              aGACCACTACCGCCAGCGGCAGGCTGCCACCGATGGCTTTGGACAAGGTGACCACATCAGGCACGATACCGGCGTGCTCGAAGG‑CAAACATGCGCCCGGTACGGGCGAAGCCGCTCTGGATCTCGTCGACGATCAACGCCAcgcccgc                                                                                                      <  3:187838/148‑1 (MQ=255)
                                               gACCACTACCGCCAGCGGCAGGCTGCCACCGATGGCTTTGGACAAGGTGACCACATCAGGCACGATACCGGCGTGCTCGAAGG‑CAAACATGCGCCCGGTACGGGCGAAGCCGCTCTGGATCTCGTCGACGATCAACGCCAcgcccgcc                                                                                                     <  6:202778/148‑1 (MQ=255)
                                                 ccACTACCGCCAGCGGCAGGCTGCCACCGATGGGTTTGGACAAGGTGACCACATCAGGCACGATACCGCCGGGCTCGAAGG‑CAAACATGCGCCCGGTACGGGCGAAGCCGCTCTGGATCTCGTCGACGATCAACGCCACGCCCGCCTGc                                                                                                  <  4:70653/149‑1 (MQ=255)
                                                  cACTACCGCCAGCGGCAGGCTGCCACCGATGGCTTTGGACAAGGTGACCACATCAGGCACGATACCGGCGTGCTCGAAGG‑CAAACATGCGCCCGGTACGGGCGAAGCCGCTCTGGATCTCGTCGACGATc                                                                                                                    >  1:6004/1‑130 (MQ=255)
                                                  cACTACCGCCAGCGGCAGGCTGCCACCGATGGCTTTGGACAAGGTGACCACATCAGGCACGATACCGGCGTGCTCGAAGG‑CAAACATGCGCCCGGTACGGGCGAAGCCGCTCTGGATCTCGTCGACGATc                                                                                                                    <  2:6004/130‑1 (MQ=255)
                                                       ccGCCAGCGGCAGGCTGCCACCGATGGCTTTGGACAAGGTGACCACATCAGGCACGATACCGGCGTGCTCGAAGG‑CAAACATGCGCCCGGTACGGGCGAAGCCGCTCTGGATCTCGTCGACGATCAACGCCACGCCCGCCTGCTCgg                                                                                              <  7:87064/147‑1 (MQ=255)
                                                        cGCCAGCGGCAGGCTGCCACCGATGGCTTTGGACAAGGTGACCACATCAGGCACGATACCGGCGTGCTCGAAGG‑CAAACATGCGCCCGGTACGGGCGAAGCCGCTCTGGATCTCGTCGACGATCAACGCCACGCCCGCCTGCTCGGTaa                                                                                           >  4:22593/1‑149 (MQ=255)
                                                         gCCAGCGGCAGGCTGCCACCGATGGCTTTGGACAAGGTGACCACATCAGGCACGATACCGGCGTGCTCGAAGG‑CAAACATGCGCCCGTACCGGGCgcat                                                                                                                                            >  2:254675/1‑96 (MQ=255)
                                                         gCCAGCGGCAGGCTGCCACCGATGGCTTTGGACAAGGTGACCACATCAGGCACCATACCGGCGTGCTCGAAGG‑CAAACATGCGCCCGTACCGGGCgcat                                                                                                                                            <  1:254675/99‑4 (MQ=255)
                                                             gCGGCAGGCTGCCACCGATGGCTTTGGACAAGGTGACCACATCAGGCACGATACCGGCGTGCTCGAAGG‑CAAACATGCGCCCGGTACGGGCGAAGCCGCTCTGGATCTCGTCGACGATCAACGCCACGCCCGCCTGCTCGGTAATgcgc                                                                                      <  3:99967/149‑1 (MQ=255)
                                                                gCAGGCTGCCACCGATGGCTTTGGACAAGGTGACCACATCAGGCACGATACCGGCGTGCTCGAAGG‑CAAACATGCGCCCGGTACGGGCGAAGCCGCTCTGGATCTCGTCGACGATCAACGCCACGCCCGCCTGCTCGGTAATGcgccgc                                                                                   <  6:129623/149‑1 (MQ=255)
                                                                gCAGGCTGCCACCGATGGCTTTGGACAAGGTGACCACATCAGGCACGATAACGGCGTGCTCGAAGG‑CAAACATGCGCCCGGTACGGGCGAAGCCGCTCTGGATCTCGTCGACGATCAactccacg                                                                                                           >  5:279020/1‑125 (MQ=255)
                                                                        ccACCGATGGCTTTGGACAAGGTGACCACATCAGGCACGATACCGGCGTGCTCGAAGG‑CAAACATGCGCCCGGTACGGGCGAAGCCGCTCTGGATCTCGTCGACGATCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCtt                                                                           >  4:337452/1‑149 (MQ=255)
                                                                              aTGGCTTTGGACAAGGTGACCACATCAGGCACGATACCGGCGTGCTCGAAGGACAAACATGCGCCCGGTACGGGCGAAGCCGCTCTGGATCTCGTCGACGATCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGc                                                                       >  1:271176/1‑148 (MQ=255)
                                                                                ggCTTTGGACATGGTTACCACATCTGGCACGATACCGGCGTGCTGGAAGC‑AAAACTTGCGCCGGGTAGGGGCGAAGCCGCTCTGGATCTCGTCGACGATCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCAtt                                                                   <  4:2993/149‑1 (MQ=255)
                                                                                        aCAAGGTGACCACATCAGGCACGATACCGGCGTGCTCGAAGG‑CAAACATGCGCCCGGTACGGGCGAAGCCGCTCTGGATCTCGTCGACGATCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCg                                                           >  5:91320/1‑149 (MQ=255)
                                                                                        aCAAGGTGACCACATCAGGCACGATACCGGCGTGCTCGAAGG‑CAAACATGCGCCCGGTACGGGCGAAGCCGCTCTGGATCTCGTCGACGATCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCg                                                           <  2:271176/149‑1 (MQ=255)
                                                                                          aaGGTGACCACATCAGGCACGATACCGGCGTGCTCGAAGG‑CAAACATGCGCCCGGTACGGGCGAAGCCGCTCTGGATCTCTTCGACGATCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCACTTGAGCCATTCGATGTCGGc                                                         >  5:73726/1‑149 (MQ=255)
                                                                                                aCCACATCAGGCACGATACCGGCGGGCTCGAAGG‑CAAACATGCGCCCGGTACGGGCGAAGCCGCTCTGGATCTCGTCGACGATCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAac                                                   <  5:94506/149‑1 (MQ=255)
                                                                                                 ccACATCAGGCACGTTACGGGCGTGCTCGAAGG‑CAAACATGCGCCCGGTACGGGCGAAGCCGCTCTGGATCTCGTCGACGATCAACGCGACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAacc                                                  <  3:312173/149‑1 (MQ=255)
                                                                                                            aCGATACCGGCGTGCTCGAAGG‑CAAACATGCGCCCGGTACGGGCGAAGCCGCTCTGGATCTCGTCGACGATCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCa                                                                                  >  8:37342/1‑106 (MQ=255)
                                                                                                                           tCGAAGG‑CAAACATGCGCCCGGTACGGGCGAAGCCGCTCTGGATCTCGTCGACGATCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAac                                                   >  5:58921/1‑122 (MQ=255)
                                                                                                                           tCGAAGG‑CAAACATGCGCCCGGTACGGGCGAAGCCGCTCTGGATCTCGTCGACGATCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAac                                                   <  6:58921/122‑1 (MQ=255)
                                                                                                                             gAAGG‑CAAACATGCGCCCGGTACGGGCGAAGCCGCTCTGGATCTCGTCGACGATCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGa                      <  3:22593/149‑1 (MQ=255)
                                                                                                                                 g‑CAAACATGCGCCCGGTACGGGCGAAGCCGGTCTGGATCTCGTCGACGATCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCtt                                                                           >  1:29554/1‑92 (MQ=255)
                                                                                                                                 g‑CAAACATGCGCCCGGTACGGGCGAAGCCGCTCTGGATCTCGTCGACGATCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCtt                                                                           <  2:29554/92‑1 (MQ=255)
                                                                                                                                 g‑CAAACATGCGCCCGGTACGGGCGAAGCCGCTCTGGATCTCGTCGACGATCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGa                   >  1:190657/1‑148 (MQ=255)
                                                                                                                                    aaaCATGCGCCCGGTACGGGCGAAGCCGCTCTGGATCTCGTCGACGATCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCa                >  7:17285/1‑149 (MQ=255)
                                                                                                                                    aaaCATGCGCCCGGTACGGGCGAAGCCGCTCTGGATCTCGTCGACGATCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCa                >  7:335041/1‑149 (MQ=255)
                                                                                                                                            gcCCGGTACGGGCGAAGCCGCTCTGGATCTCGTCGACGATCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCAcggcggcg        <  6:244076/149‑1 (MQ=255)
                                                                                                                                             cccGGTACGGGCGAAGCCGCTCTGGATCTCGTCGACGATCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCAcggcggcgt       >  3:234965/1‑148 (MQ=255)
                                                                                                                                                  tACGGGCGAAGCCGCTCTGGATCTCGTCGACGATCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGc  >  3:246417/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GTACCGGCATGCGCACCTGGCTTCCAGGTGTCCAGCCAGTCGCGATAGACCACTACCGCCAGCGGCAGGCTGCCACCGATGGCTTTGGACAAGGTGACCACATCAGGCACGATACCGGCGTGCTCGAAGG‑CAAACATGCGCCCGGTACGGGCGAAGCCGCTCTGGATCTCGTCGACGATCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGC  >  NC_002947/4795499‑4795792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑