Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 363,360 G→A 6.6% G41G (GGC→GGT)  lcdH ← L‑carnitine dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947363,3600GA 6.6% 176.5 / 2.3 60G41G (GGC→GGT) lcdHL‑carnitine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (23/33);  new base A (2/2);  total (25/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.50e-01

CTTTCCTGGATGAAATCGGCGTTGCGCACGCATTCCTCGATGGTTGCCACGAACTTCAGGCGGTCTTGCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCGGCGAAGGTTTTGATTTCAGTGATAAAGGGCATAGCTGGTCCTCAGGGTAAAACACAG  >  NC_002947/363214‑363507
                                                                                                                                                    |                                                                                                                                                   
cTTTCCTGGATGAAATCGGCGTTGCGCACGCATTCCTCGATCGTTGCCACGAACTTCAGGCGGTCTTGCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCggcgcc                                                                                                                                                   <  5:315136/149‑1 (MQ=255)
 tttCCTGGATGAAATCGGCGTTGCGCACGCATTCCTCGATGGTTGCCACGAACTTCAGGCGGTCTTGCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCggcgccg                                                                                                                                                  >  3:45240/1‑149 (MQ=255)
      tGGATGAAATCGGCGTTGCGCACGCATTCCTCGATGGTTGCCACGAACTTCAGGCGGTCTTGCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCggcgccgggcgc                                                                                                                                             >  3:289042/1‑149 (MQ=255)
      tGGATGAAATCGGCGTTGCGCACGCATTCCTCGATGGTTGCCACGAACTTCAGGCGGTCTTGCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCggcgccgggcgc                                                                                                                                             >  2:354112/1‑149 (MQ=255)
       ggATGAAATCGGCGTTGCGCACGCATTCCTCGATGGTTGCCACGAACTTCAGGCGGTCTTGCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCggcgccgggcgcc                                                                                                                                            >  7:280565/1‑149 (MQ=255)
         aTGAAATCGGCGTTGCGCACGCATTCCTCGATGGTTGCCACGAACTTCAGGCGGTCTTGCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCggcgcccggcgccg                                                                                                                                           >  2:53363/1‑148 (MQ=255)
          tGAAATCGGCGTTGCGCACGCATTCCTCGATGGTTGCCACGAACTTCAGGCGGTCTTGCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCGgcgccgggcgccgg                                                                                                                                          >  8:101772/1‑148 (MQ=255)
          tGAAATCGGCGTTGCGCACGCATTCCTCGATGGTTGCCACGAACTTCAGGCGGTCTTGCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCGgcgccgggcgccgg                                                                                                                                          <  7:101772/148‑1 (MQ=255)
             aaTCGGCGTTGCGCACGCATTCCTCGATGGTTGCCACGAACTTCAGGCGGTCTTGCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCggcgcccggcgccgaacag                                                                                                                                      <  1:53363/149‑6 (MQ=255)
              aTCGGCGTTGCGCACGCATTCCTCGATGGTTGCCACGAACTTCAGGCGGTCTTGCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCggcgccgg                                                                                                                                                 <  4:145094/137‑1 (MQ=255)
              aTCGGCGTTGCGCACGCATTCCTCGATGGTTGCCACGAACTTCAGGCGGTCTTGCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCggcgccgg                                                                                                                                                 >  3:145094/1‑137 (MQ=255)
              aTCGGCGTTGCGCACGCATTCCTCGATGGTTGCCACGAACTACAGGCGGTCTTGCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTcc                                                                                                                                      >  2:116515/1‑148 (MQ=255)
               tCGGCGTTGCGCACGCATTCCTCGATGGTTGCCACGAACTTCAGGCGGTCTTGCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCa                                                                                                                                    <  6:89558/149‑1 (MQ=255)
                 ggCGTTGCGCACGCATTCCTCGATGGTTGCCACGAACTTCAGGCGGTCTTGCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAg                                                                                                                                   <  4:30563/148‑1 (MQ=255)
                  gCGTTGCGCACGCATTCCTCGATGGTTGCCACGAACTTCAGGCGGTCTTGCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGc                                                                                                                                 >  8:74712/1‑149 (MQ=255)
                   cGTTGCGCACGCATTCCTCGATGGTTGCCACGAACTTCAGGCGGTCTTGCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGcc                                                                                                                                <  6:225651/149‑1 (MQ=255)
                     ttGCGCACGCATTCCTCGATGGTTGCCACGAACTTCAGGCGGTCTTGCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGccac                                                                                                                              <  5:190002/149‑1 (MQ=255)
                         gcacgcaTTCCTCGATGGTTGCCACGAACTTCAGGCGGTCTTGCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACcaca                                                                                                                          >  1:352630/1‑149 (MQ=255)
                              caTTCCTCGATGGTTGCCACGAACTTCAGGCGGTCTTGCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAg                                                                                                                     <  7:281255/149‑1 (MQ=255)
                               aTTCCTCGATGGTTGCCACGAACTTCAGGCGGTCTTGCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTGTTGCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCa                                                                                                                      <  1:284830/149‑1 (MQ=255)
                               aTTCCTCGATGGTTGCCACGAACTTCAGGCGGTCTTGCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAgg                                                                                                                    >  7:320869/1‑149 (MQ=255)
                                                aCGAACTTCAGGCGGTCTTGCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCa                                                                                                                 >  2:282492/1‑135 (MQ=255)
                                                aCGAACTTCAGGCGGTCTTGCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCa                                                                                                                 <  1:282492/135‑1 (MQ=255)
                                                aCGAACTTCAGGCGGTCTTGCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAgg                                                                                                        >  4:265956/1‑144 (MQ=255)
                                                aCGAACTTCAGGCGGTCTTGCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAgg                                                                                                        <  3:265956/144‑1 (MQ=255)
                                                      ttCAGGCGGTCTTGCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCTAGGGCGCGGGCGAc                                                                                             >  3:63489/1‑149 (MQ=255)
                                                         aGGCGGTCTTGCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGAccc                                                                                           <  8:280565/148‑1 (MQ=255)
                                                          ggCGGTCTTGCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAg                                                                                         <  8:320869/149‑1 (MQ=255)
                                                          ggCGGTCTTGCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAg                                                                                         >  6:85702/1‑149 (MQ=255)
                                                              gTCTTGCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGc                                                                                     <  1:354112/149‑1 (MQ=255)
                                                                   gCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGTGCCAGGGCGCGGGCGACCCAGCCGCTGc                                                                                  <  4:177329/147‑1 (MQ=255)
                                                                   gCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCTAGGGCGCGGGCGACCCAGCCGCTGc                                                                                  <  4:63489/147‑1 (MQ=255)
                                                                   gCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGcc                                                                                 >  4:155515/1‑148 (MQ=255)
                                                                     gATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGAt                                                                              >  4:98461/1‑149 (MQ=255)
                                                                       tGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCa                                                                            <  3:155515/149‑1 (MQ=255)
                                                                          gcCAGGGTCCAGTCCTTGTTTTTCCAGTGCCGGTCAGTCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCCGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGTCCATGGGCTAGGGCGCGGGCGACCCAGACGCTGCCGATCAcgc                                                                         <  5:22904/149‑1 (MQ=255)
                                                                                ggCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTAc                                                                   <  4:45240/149‑1 (MQ=255)
                                                                                 gCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTAcc                                                                  >  2:313284/1‑149 (MQ=255)
                                                                                   cAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCTAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTAcc                                                                  >  3:243996/1‑147 (MQ=255)
                                                                                    aGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAg                                                               >  3:187410/1‑149 (MQ=255)
                                                                                       ccTTGTTTTTCCAGTGCCGGCCAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGggc                                                            >  5:353711/1‑149 (MQ=255)
                                                                                           gTTTTTCCAGTGCCGGCCAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTa                                                                    <  5:262064/137‑1 (MQ=255)
                                                                                                   aGTGCCGGCCAGGCGTTG‑‑TCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCGGCGAAGGtttt                                                <  4:273546/149‑1 (MQ=255)
                                                                                                     tGCCGGCCAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCTAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCGGCGAAGGTTTTGa                                              >  6:155050/1‑149 (MQ=255)
                                                                                                          gCCAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCGGCGAAGGTTTTGATTTCa                                         <  1:220309/149‑1 (MQ=255)
                                                                                                          gCCAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCGGCGAAGGTTTTGATTTCa                                         <  4:289042/149‑1 (MQ=255)
                                                                                                           ccAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCTAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCGGCGAAGGTTTTGATTTCAg                                        <  5:155050/149‑1 (MQ=255)
                                                                                                           ccAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCTAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCGGCGAAGGTTTTGATTTCAg                                        <  4:243996/149‑1 (MQ=255)
                                                                                                                  ttG‑‑GCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCGGCGAAGGTTTTGATTTCAGTGATaaa                                 <  3:98461/149‑1 (MQ=255)
                                                                                                                       gCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAAGGCGGCGAAGGTTTTGATTTCAGTGATAAAggg                              <  5:85702/149‑1 (MQ=255)
                                                                                                                         gACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCGGCGAAGGTTTTGATTTCAg                                        <  6:952/137‑1 (MQ=255)
                                                                                                                         gACACGTTTGCGCAGTACCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCGGCGAAGGTTTTGa                                              >  7:228714/1‑131 (MQ=255)
                                                                                                                            acGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCGGCGAAGGTTTTGATTTCAGTGATAAAGGGCATAg                         >  8:64675/1‑149 (MQ=255)
                                                                                                                              gTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCGGCGAAGGTTTTGATTTCAGTg                                      >  4:90560/1‑134 (MQ=255)
                                                                                                                              gTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCGGCGAAGGTTTTGATTTCAGTg                                      <  3:90560/134‑1 (MQ=255)
                                                                                                                               tttGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCGGCGAAGGTTTTGATTTCAGTGAAAAAGGGCATAGCTg                      >  8:132307/1‑149 (MQ=255)
                                                                                                                                  gcgcAGTGCCTGTTCGGCACCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCGGCGAAGGTTTTGATTTCAGTGATAAAg                                <  5:333759/136‑1 (MQ=255)
                                                                                                                                  gcgcAGTGCCTGTTCGGCACCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCGGCGAAGGTTTTGATTTCAGTGATAAAg                                >  6:333759/1‑136 (MQ=255)
                                                                                                                                    gcAGTGCCTGTTCTGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCGGCGAAGGTTTTGATTTCAGTGATAAAGGGCATAGCTGGTCCt                 >  5:13651/1‑149 (MQ=255)
                                                                                                                                     cAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCGGCGAAGGTTTTGATTTCAGTGATAAAGGGCATAGCTGGTCCTc                <  4:238318/149‑1 (MQ=255)
                                                                                                                                     cAGTGCCTGTTCGGCACCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCGGCGAAGGTTTTGATTTCAGTGATAAAGGGCATAGCTGGTCCt                 >  8:137177/1‑148 (MQ=255)
                                                                                                                                      aGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCGGCGAAGGTTTTGATTTCAGTGATAAAGGGCATAGCTGGTCCt                 >  5:305974/1‑147 (MQ=255)
                                                                                                                                      aGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCGGCGAAGGTTTTGATTTCAGTGATAAAGGGCATAGCTGGTCCTCa               <  7:64675/149‑1 (MQ=255)
                                                                                                                                      aGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCGGCGAAGGTTTTGATTTCAGTGATAAAGGGCATAGCTGGTCCTCa               <  5:201062/149‑1 (MQ=255)
                                                                                                                                       gTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCGGCGAAGGTTTTGATTTCAGTGATAAAGGGCATAGCTGGTCCTCAg              <  6:13651/149‑1 (MQ=255)
                                                                                                                                        tGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCTAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCGGCGAAGGTTTTGATTTCAGTGATAAAGGGCATAGCTGGTCCTCAgg             <  7:74712/149‑1 (MQ=255)
                                                                                                                                        tGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCGGCGAAGGTTTTGATTTCAGTGATAAAGGGCATAGCTGGTCCTCAgg             <  6:353711/149‑1 (MQ=255)
                                                                                                                                        tGCCTGTTCGGCACCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCGGCGAAGGTTTTGATTTCAGTGATAAAGGGCATAGCTGGTCCTCAgg             <  7:137177/149‑1 (MQ=255)
                                                                                                                                            tGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCTAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCGGCGAAGGTTTTGATTTCa                                         >  4:274948/1‑117 (MQ=255)
                                                                                                                                            tGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCTAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCGGCGAAGGTTTTGATTTCa                                         <  3:274948/117‑1 (MQ=255)
                                                                                                                                            tGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCGGCGAAGGTTTTGATTTCAGTGATAAAGGGCATAGATGGTCCTCAGGGTa          >  6:4181/1‑148 (MQ=255)
                                                                                                                                                  gcgccgggcgccggGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCGGCGAAGGTTTTGATTTc                                          >  2:150911/1‑110 (MQ=255)
                                                                                                                                                  gcgccgggcgccggGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCGGCGAAGGTTTTGATTTc                                          <  1:150911/110‑1 (MQ=255)
                                                                                                                                                   cgccgggcgccgggTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCGGCGAAGGTTTTGATTTCAGTGATAAAGGGCATAGCTGGTCCTCAGGGTAAAACACAg  <  1:313284/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CTTTCCTGGATGAAATCGGCGTTGCGCACGCATTCCTCGATGGTTGCCACGAACTTCAGGCGGTCTTGCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTG‑‑GCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCGGCGAAGGTTTTGATTTCAGTGATAAAGGGCATAGCTGGTCCTCAGGGTAAAACACAG  >  NC_002947/363214‑363507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑