Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,820,436 C→T 8.9% G3585D (GGC→GAC)  pvdL ← non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,820,4360CT 8.9% 122.4 / 2.4 45G3585D (GGC→GAC) pvdLnon‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base C (20/21);  new base T (2/2);  total (22/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.30e-01

ATCACCCGGGTAACACACCGCCGTCAGCGGGAAGTTGGTGTGGGTACGGCCCGAGTCGGAGCTGGCGTTCAGGTGCTGGGCGTGATTGAGCACGGCGGTTTCAACCGGCGCATTCTCGAACACGAACAGGCTGTCGAACAGCGGCTGGCCCTTGGGCAGCTCGCTGCACGCCTGGATCGCCACCAGCGGCAGGTATTCGTACTCGCGCAGCTCCATGTTGCTTTGCAGCAGGCCTTGCAGCCACTGGCGCACACTGCAGCGCTCACTCGCCGCAGGCAACTGCACACGCAGGGC  >  NC_002947/4820288‑4820581
                                                                                                                                                    |                                                                                                                                                 
atcaCCCGGGTAACACACCGCCGTCAGCGGGAAGTTGGTGTGGGTACGGCCCGAGTCGGAGCTGGCGTTCAGGTGCTGGGCGTGATTGAGCACGGCGGTTTCAACCGGCGCATTCTCGAACACGAACAGGCTGTCGAACAGCGGCTGGc                                                                                                                                                   >  3:86284/1‑149 (MQ=255)
     ccGGGTAACACACCGCCGTCAGCGGGAAGTTGGTGTGGGTACGGCCCGAGTCGGAGCTGGCGTTCAGGTGCTGGGCGTGATTGAGCACGGCGGTTTCAACCGGCGCATTCTCGAACACGAACAGGCTGTCGAACAGCGGCTGGCCCTTg                                                                                                                                              <  5:136891/149‑1 (MQ=255)
       gggTAACACACCGCCGTCAGCGGGAAGTTGGTGTGGGTACGGCCCGAGTCGGAGCTGGCGTTCAGGTGCTGGGCGTGATTGAGCACGGCGGTTTCAACCGGCGCATTCTCGAACACGAACAGGCTGTCGAACAGCGGCTGGCCCTTggg                                                                                                                                            <  4:94792/149‑1 (MQ=255)
        ggTAACACACCGCCGTCAGCGGGAAGTTGGTGTGGGTACGGCCCGAGTCGGAGCTGGCGTTCAGGTGCTGGGCGTGATTGAGCACGGCGGTTTCAACCGGCGCATTCTCGAACACGAACAGGCTGTCGAACAGCGGCTGGCCCTTggg                                                                                                                                            >  3:269966/1‑148 (MQ=255)
              acacCGCCGTCAGCGGGAAGTTGGTGTGGGTACGGCCCGAGTCGGAGCTGGCGTTCAGGTGCTGGGCGTGATTGAGCACGGCGGTTTCAACCGGCGCATTCTCGAACACGAACAGGCTGTCGAACAGCGGCTGGCCCTTGGGCAGCTCg                                                                                                                                     >  7:121849/1‑149 (MQ=255)
               cacCGCCGTCAGCGGGAAGTTGGTGTGGGTACGGCCCGAGTCGGAGCTGGCGTTCAGGTGCTGGGCGTGATTGAGCACGGCGGTTTCAACCGGCGCATTCTCGAACACGAACAGGCTGTCGAACAGCGGCTGGCCCTTGGGCAGCTCGc                                                                                                                                    >  6:182463/1‑149 (MQ=255)
                              gAAGTTGGTGTGGGTACGGCCCGAGTCGGAGCTGGCGTTCAGGTGCTGGGCGTGATTGAGCACGGCGGTTTCAACCGGCGCATTCTCGAACACGAACAGGCTGTCGAACAGCGGCTGGCCCTTGGGCAGCTCGCTGCACGCCTGGATCg                                                                                                                     >  3:351541/1‑149 (MQ=255)
                              gAAGTTGGTGTGGGTACGGCCCGAGTCGGAGCTGGCGTTCAGGTGCTGGGCGTGATTGAGCACGGCGGTTTCAACCGGCGCATTCTCGAACACGAACAGGCTGTCGAACAGCGCCTGGCCCTAGGGCAGCTCGCTGCACGCCTGGATCg                                                                                                                     >  7:192264/1‑149 (MQ=255)
                                      tgtgGGTACGGCCCGAGTCGGAGCTGGCGTTCAGGTGCTGGGCGTGATTGAGCACGGCGGTTTCAACCGGCGCATTCTCGAACACGAACAGGCTGTCGAACAGCGGCTGGCCCTTGGGCAGCTCGCTGCACGCCTGGATCGCCACCAGc                                                                                                             <  4:269966/149‑1 (MQ=255)
                                       gtgGGTACGGCCCGAGTCGGAGCTGGCGTTCAGGTGCTGGGCGTGATTGAGCACGGCGGTTTCAACCGGCGCATTCTCGAACACGAACAGGCTGTCGAACAGCGGCTGGCCCTTGGGCAGCTCGCTGCACGCCTGGATCGcc                                                                                                                   <  1:64868/142‑1 (MQ=255)
                                       gtgGGTACGGCCCGAGTCGGAGCTGGCGTTCAGGTGCTGGGCGTGATTGAGCACGGCGGTTTCAACCGGCGCATTCTCGAACACGAACAGGCTGTCGAACAGCGGCTGGCCCTTGGGCAGCTCGCTGCACGCCTGGATCGcc                                                                                                                   >  2:64868/1‑142 (MQ=255)
                                        tgGGTACGGCCCGAGTCGGAGCTGGCGTTCAGGTGCTGGGCGTGATTGAGCACGGCGGTTTCAACCGGCGCATTCTCGAACACGAACAGGCTGTCGAACAGCGGCTGGCCCTTGGGCAGCTCGCTGCACGCCTGGATCGCCACCAGCgg                                                                                                           >  7:230286/1‑149 (MQ=255)
                                           gTACGGCCCGAGTCGGAGCTGGCGTTCAGGTGCTGGGCGTGATTGAGCACGGCGGTTTCAACCGGCGCATTCTCGAACACGAACAGGCTGTCGAACAGCGGCTGGCCCTTGGGCAGCTCGCTGCACGCCTGGATCGCCACCAGCGGCAg                                                                                                        <  2:56491/149‑1 (MQ=255)
                                               ggCCCGAGTCGGAGCTGGCGTTCAGGTGCTGGGCGTGATTGAGCACGGCGGTTTCAACCGGCGCATTCTCGAACACGAACAGGCTGTCGAACAGCGGCTGGTCCTTGGGCAGCTCGCTGCACGCCTGGATCGCCACCAGCGGCAGGTa                                                                                                     >  6:209129/1‑148 (MQ=255)
                                               ggCCCGAGTCGGAGCTGGCGTTCAGGTGCTGGGCGTGATTGAGCACGGCGGTTTCAACCGGCGCATTCTCGAACACGAACAGGCTGTCGAACAGCGGCTGGTCCTTGGGCAGCTCGCTGCACGCCTGGATCGCCACCAGCGGCAGGTa                                                                                                     >  2:335847/1‑148 (MQ=255)
                                               ggCCCGAGTCGGAGCTGGCGTTCAGGTGCTGGGCGTGATTGAGCACGGCGGTTTCAACCGGCGCATTCTCGAACACGAACAGGCTGTCGAACAGCGGCTGGTCCTTGGGCAGCTCGCTGCACGCCTGGATCGCCACCAGCGGCAGGTa                                                                                                     <  1:335847/148‑1 (MQ=255)
                                               ggCCCGAGTCGGAGCTGGCGTTCAGGTGCTGGGCGTGATTGAGCACGGCGGTTTCAACCGGCGCATTCTCGAACACGAACAGGCTGTCGAACAGCGGCTGGTCCTTGGGCAGCTCGCTGCACGCCTGGATCGCCACCAGCGGCAGGTa                                                                                                     <  5:209129/148‑1 (MQ=255)
                                                 cccGCGTCGGAGCGGGCGTTCCGGTGCTGGGCGTGATTGAGCACGGCGGTTTCAACGGGCGCATTCTCGAACACGAACAGGCTGTGGAACAGCGGCTGGCCCTTGGGCAGCTCGCTGCACGCCTGGATCGCCACCAGCGGCAGGTATTc                                                                                                  <  8:121849/149‑1 (MQ=39)
                                                          gAGCTGGCGTTCAGGTGCTGGGCGTGATTGAGCACGGCGGTTTCAACCGGCGCATTCTCGAACACGAACAGGCTGTCGAACAGCGGCTGGCCCTTGGGCAGCTCGCTGCACGCCTGGATCGCCACCAGCGGCAGGTATTCGTACTcgcg                                                                                         >  3:118677/1‑149 (MQ=255)
                                                          gAGCTGGCGTTCAGGTGCTGGGCGTGATTGAGCACGGCGGTTTCAACCGGCGCATTCTCGAACACGAACAGGCTGTCGAACAGCGGCTGGCCCTTGGGCAGCTCGCTGCACGCCTGGATCGCCACCAGCGGCAGGTATTCGTACTcgcg                                                                                         <  8:230286/149‑1 (MQ=255)
                                                                  gTTCAGGTGCTGGGCGTGATTGAGCACGGCGGTTTCAACCGGCGCATTCTCGAACACGAACAGGCTGTCGAACAGCGGCTGGCCCTTGGGCAGCTCGCTGCACGCCTGGATCGCCACCAGCGGCAGGTATTCGTACTcgc                                                                                          <  4:121919/140‑1 (MQ=255)
                                                                  gTTCAGGTGCTGGGCGTGATTGAGCACGGCGGTTTCAACCGGCGCATTCTCGAACACGAACAGGCTGTCGAACAGCGGCTGGCCCTTGGGCAGCTCGCTGCACGCCTGGATCGCCACCAGCGGCAGGTATTCGTACTcgc                                                                                          >  3:121919/1‑140 (MQ=255)
                                                                   ttCAGGTGCTGGGCGTGATTGAGCACGGCGGTTTCAACCGGCGCATTCTCGAACACGAACAGGCTGTCGAACAGCGGCTGGCCCTTGGGCAGCTCGCTGCACGCCTGGATCGCCACCAGCGGCAGGTATTCGTACTCGCGCAGCTCCAt                                                                                >  6:163015/1‑149 (MQ=255)
                                                                      aGGTGCTGGGCGTGATTGAGCACGGCGGTTTCAACCGGCGCATTCTCGAACACGAACAGGCTGTCGAACAGCGGCTGGCCCTTGGGCAGCTCGCTGCACGCCTGGATCGCCACCAGCGGCAGGTATTCGTACTCGCGCAGCTCCATGtt                                                                             >  1:175390/1‑149 (MQ=255)
                                                                      aGGTGCTGGGCGTGATTGAGCACGGCGGTTTCAACCGGCGCATTCTCGAACACGAACAGGCTGTCGAACAGCGGCTGGCCCTTGGGCAGCTCGCTGCACGCCTGGATCGCCACCAGCGGCAGGTATTCGTACTCGCGCAGCTCCATGt                                                                              <  4:86284/148‑1 (MQ=255)
                                                                              ggCGTGATTGAGCACGGCGGTTTCAACCGGCGCATTCTCGAACACGAACAGGCTGTCGAACAGCGGCTGGCCCTTGGGCAGCTCGCTGCACGCCTGGATCGCCACCAGCGGCAGGTATTCGTACTCGCGCAGCTCCATGTTGCTTTgca                                                                     <  8:255164/149‑1 (MQ=255)
                                                                              ggCGTGATTGAGCACGGCGGTTTCAACCGGCGCATTCTCGAACACGAACAGGCTGTCGAACAGCGGCTGGCCCTTGGGCAGCTCGCTGCACGCCTGGATCGCCACCAGCGGCAGGTATTCGTACTCGCGCAGCTCCATGTTGCTTTg                                                                       <  5:182463/147‑1 (MQ=255)
                                                                                    aTTGAGCACGGCGGTTTCAACCGGCGCATTCTCGAACACGAACAGGCTGTCGAACAGCGGCTGGCCCTTGGGCAGCTCGCTGCACGCCTGGATCGCCACCAGCGGCAGGTATTCGTACTCGCGCAGCTCCATGTTGCTTTGCAGCAGGc                                                               <  2:175390/149‑1 (MQ=255)
                                                                                        aGCACGTCGGTTTCAACCGGCGCATTCTCGAACACGAACAGGCTGTCGAACAGCGGCTGGCCCTTGGGCAGCTCGCTGCACGCCTGGATCGCCACCAGCGGCAGGTATTCGTACTCGCGCAGCTCCATGTTGCTTTGCAGCAGGCCTTg                                                           <  7:344887/149‑1 (MQ=255)
                                                                                          cACGGCGGTTTCTACCGGCGCATTCTCGAACACGAACAGGCTGTCGAACAGCGGCTGGCCCTTGGGCAGCTCGCTGCACGCCTGGATCGCCACCAGCGGCAGGTATTCGTACTCGCGCAGCTCCATGTTGCTTTGCAGCAGGCCTTGCa                                                         <  5:163015/149‑1 (MQ=255)
                                                                                                      aaCCGGCGCATTCTCGAACACGAACAGGCTGTCGAACAGCGGCTGGCCCTTGGGCAGCTCGCTGCACGCCTGGATCGCCACCAGCGGCAGGTATTCGTACTCGCGCAGCTCCATGTTGCTTTGCAGCAGGCCTTGCAGCCACTGGCGca                                             >  8:221603/1‑149 (MQ=255)
                                                                                                      aaCCGGCGCATTCTCGAACACGAACAGGCTGTCGAACAGCGGCTGGCCCTTGGGCAGCTCGCTGCACGCCTGGATCGCCACCAGCGGCAGGTATTCGTACTCGCGCAGCTCCATGTTGCTTTGCAGCAGGCCTTGCAGCCACTGGCGca                                             >  2:65422/1‑149 (MQ=255)
                                                                                                                     gaacacgaacaGGCTGTCGAACAGCGGCTGGCCCTTGGGCAGCTCGCTGCACGCCTGGATCGCCACCAGCGGCAGGTATTCGTACTCGCGCAGCTCCATGTTGCTTTGCAGCAGGCCTTGCAGCCACTGGCGCACACTGCAGCGCTCAc                              >  5:252645/1‑149 (MQ=255)
                                                                                                                          cgaacaGGCTGTCGAACAGCGGCTGGCCCTTGGGCAGCTCGCTGCACGCCTGGATCGCCACCAGCGGCAGGTATTCGTACTCGCGCAGCTCCATGTTGCTTTGCAGCAGGCCTTGCAGCCACTGGCGCACACTGCAGCGCTCACTcgcc                         <  4:118677/149‑1 (MQ=255)
                                                                                                                           gaacaGGCTGTCGAACAGCGGCTGGCCCTTGGGCAGCTCGCTGCACGCCTGGATCGCCACCAGCGGCAGGTATTCGTACTCGCGCAGCTCCATGTTGCTTTGCAGCAGGCCTTGCAGCCACTgg                                                 >  1:310277/1‑124 (MQ=255)
                                                                                                                           gaacaGGCTGTCGAACAGCGGCTGGCCCTTGGGCAGCTCGCTGCACGCCTGGATCGCCACCAGCGGCAGGTATTCGTACTCGCGCAGCTCCATGTTGCTTTGCAGCAGGCCTTGCAGCCACTgg                                                 <  2:310277/124‑1 (MQ=255)
                                                                                                                             acaGGCTGTCGAACAGCGGCTGGCCCTTGGGCAGCTCGCTGCACGCCTGGATCGCCACCAGCGGCAGGTATTCGTACTCGCGCAGCTCCATGTTGCTTTGCAGCAGGCCTTGCAGCCACTGGCGCACACTGCAGCGCTCACTCGCCGCa                      <  4:351541/149‑1 (MQ=255)
                                                                                                                                 gCTGTCGAACAGCGGCTGGCCCTTGGGCAGCTCGCTGCACGCCTGGATCGCCACCAGCGGCAGGTATTCGTACTCGCGCAGCTCCATGTTGCTTTGCAGCAGGCCTTGCAGCCACTGGCGCACACTGCCGCGCTCACTCGCCGCAGGc                   >  6:20920/1‑148 (MQ=255)
                                                                                                                                     tccaaCAGCGGCTGGCCCTTGGGCAGCTCGCTGCACGCCTGGATCGCCACCAGCGGCAGGTATTCGTACTCGCGCAGCTCCATGTTGCTTTGCAGCAGGCCTTGCAGCCACTGGCGCACACTGCAGCGCTCACTCGCCGCAGGCAACTg              <  5:20920/146‑1 (MQ=255)
                                                                                                                                       gAACAGCGGCTGGCCCTTGGGCAGCTCGCTGCACGCCTGGATCGCCACCAGCGGCAGGTATTCGTACTCGCGCAGCTCCATGTTGCTTTGCAGCAGGCCTTGCAGCCACTGGCGCACACTGCAGCGCTCACTCGCCGCAGGCAACTGca            <  7:221603/149‑1 (MQ=255)
                                                                                                                                            gCGGCTGGCCCTTGGGCAGCTCGCTGCACGCCTGGATCGCCACCAGCGGCAGGTATTCGTACTCGCGCAGCTCCATGTTGCTTTGCAGCAGGCCTTGCAGCCACTGGCGCACACTGCAGCGCTCACTCGCCGCAGGCAACTGCACACGc       <  6:111812/149‑1 (MQ=255)
                                                                                                                                              ggCTGGCCCTTGGGCAGCTCGCTGCACGCCTGGATCGCCACCAGCGGCAGGTATTCGTACTCGCGCAGCTCCATGTTGCTTTGCAGCAGGCCTTGCAGCCACTGGCGCACACTGCAGCGCTCACTCGCCGCAGGCAACTGCACACGCAg     >  2:184117/1‑149 (MQ=255)
                                                                                                                                                cTGGCCCTTGGGCAGCTCGCTGCACGCCTGGATCGCCACCAGCGGCAGGTATTCGTACTCGCGCAGCTCCATGTTGCTTTGCAGCAGGCCTTGCAGCCACTGGCGCACACTGCAGCGCTCACTCGCCGCAGGCAACTGCACACGCAggg   >  2:90696/1‑149 (MQ=255)
                                                                                                                                                 tGGCCCTTGGGCAGCTCGCTGCACGCCTGGATCGCCACCAGCGGCAGGTATTCGTACTCGCGCAGCTCCATGTTGCTTTGCAGCAGGCCTTGCAGCCACTGGCGCACACTGCAGCGCTCACTCGCCGCAGGCAACTGCACACGCAGGGc  >  1:40314/1‑149 (MQ=255)
                                                                                                                                                   gCCCTTGGGCAGCTCGCTGCACGCCTGGATCGCCACCAGCGGCAGGTATTCGTACTCGCGCAGCTCCATGTTGCTTTGCAGCAGGCCTTGCAGCCACTGGCGCACACTGCAGCGCTCACTCGCCGCAGGCaa                 <  6:237047/132‑1 (MQ=255)
                                                                                                                                                   gCCCTTGGGCAGCTCGCTGCACGCCTGGATCGCCACCAGCGGCAGGTATTCGTACTCGCGCAGCTCCATGTTGCTTTGCAGCAGGCCTTGCAGCCACTGGCGCACACTGCAGCGCTCACTCGCCGCAGGCaa                 >  5:237047/1‑132 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
ATCACCCGGGTAACACACCGCCGTCAGCGGGAAGTTGGTGTGGGTACGGCCCGAGTCGGAGCTGGCGTTCAGGTGCTGGGCGTGATTGAGCACGGCGGTTTCAACCGGCGCATTCTCGAACACGAACAGGCTGTCGAACAGCGGCTGGCCCTTGGGCAGCTCGCTGCACGCCTGGATCGCCACCAGCGGCAGGTATTCGTACTCGCGCAGCTCCATGTTGCTTTGCAGCAGGCCTTGCAGCCACTGGCGCACACTGCAGCGCTCACTCGCCGCAGGCAACTGCACACGCAGGGC  >  NC_002947/4820288‑4820581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑