Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	363,402	C→T	14.9%	L27L (CTG→CTA)	lcdH ←	L‑carnitine dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	363,402	0	C	T	14.9%	157.1 / 17.3	67	L27L (CTG→CTA)	lcdH	L‑carnitine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (25/32);  new base T (4/6);  total (29/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.43e-01

ACGAACTTCAGGCGGTCTTGCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTGGCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGATTTCAGTGATAAAGGGCATAGCTGGTCCTCAGGGTAAAACACAGTAATCAGGGTTCATGGCTAACCCTGTGGGAGCGGGCATGCCC  >  NC_002947/363262‑363549                                                                                                                                             |                                                                                                                                                      aCGAACTTCAGGCGGTCTTGCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTGGCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAgg                                                                                                                                                    >  4:265956/1‑144 (MQ=255) aCGAACTTCAGGCGGTCTTGCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTGGCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAgg                                                                                                                                                    <  3:265956/144‑1 (MQ=255)       ttCAGGCGGTCTTGCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTGGCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCTAGGGCGCGGGCGAc                                                                                                                                         >  3:63489/1‑149 (MQ=255)          aGGCGGTCTTGCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTGGCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGAccc                                                                                                                                       <  8:280565/148‑1 (MQ=255)           ggCGGTCTTGCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTGGCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAg                                                                                                                                     <  8:320869/149‑1 (MQ=255)           ggCGGTCTTGCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTGGCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAg                                                                                                                                     >  6:85702/1‑149 (MQ=255)               gTCTTGCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTGGCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGc                                                                                                                                 <  1:354112/149‑1 (MQ=255)                    gCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTGGCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGTGCCAGGGCGCGGGCGACCCAGCCGCTGc                                                                                                                              <  4:177329/147‑1 (MQ=255)                    gCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTGGCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCTAGGGCGCGGGCGACCCAGCCGCTGc                                                                                                                              <  4:63489/147‑1 (MQ=255)                    gCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTGGCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGcc                                                                                                                             >  4:155515/1‑148 (MQ=255)                      gATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTGGCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGAt                                                                                                                          >  4:98461/1‑149 (MQ=255)                        tGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTGGCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCa                                                                                                                        <  3:155515/149‑1 (MQ=255)                           gcCAGGGTCCAGTCCTTGTTTTTCCAGTGCCGGTCAGTCGTTGGCGACACGTTTGCGCAGTGCCTGTTCCGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGTCCATGGGCTAGGGCGCGGGCGACCCAGACGCTGCCGATCAcgc                                                                                                                     <  5:22904/149‑1 (MQ=255)                                 ggCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTGGCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTAc                                                                                                               <  4:45240/149‑1 (MQ=255)                                  gCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTGGCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTAcc                                                                                                              >  2:313284/1‑149 (MQ=255)                                    cAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTGGCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCTAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTAcc                                                                                                              >  3:243996/1‑147 (MQ=255)                                     aGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTGGCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAg                                                                                                           >  3:187410/1‑149 (MQ=255)                                        ccTTGTTTTTCCAGTGCCGGCCAGGCGTTGGCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGggc                                                                                                        >  5:353711/1‑149 (MQ=255)                                            gTTTTTCCAGTGCCGGCCAGGCGTTGGCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTa                                                                                                                <  5:262064/137‑1 (MQ=255)                                                    aGTGCCGGCCAGGCGTTGTCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGtttt                                                                                          <  4:273546/149‑1 (MQ=255)                                                      tGCCGGCCAGGCGTTGGCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCTAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGa                                                                                        >  6:155050/1‑149 (MQ=255)                                                           gCCAGGCGTTGGCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGATTTCa                                                                                   <  4:289042/149‑1 (MQ=255)                                                           gCCAGGCGTTGGCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGATTTCa                                                                                   <  1:220309/149‑1 (MQ=255)                                                            ccAGGCGTTGGCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCTAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGATTTCAg                                                                                  <  5:155050/149‑1 (MQ=255)                                                            ccAGGCGTTGGCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCTAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGATTTCAg                                                                                  <  4:243996/149‑1 (MQ=255)                                                                   ttGGCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGATTTCAGTGATaaa                                                                           <  3:98461/149‑1 (MQ=255)                                                                      gCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAAGGCG‑‑GCGAAGGTTTTGATTTCAGTGATAAAggg                                                                        <  5:85702/149‑1 (MQ=255)                                                                        gACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGATTTCAg                                                                                  <  6:952/137‑1 (MQ=255)                                                                        gACACGTTTGCGCAGTACCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGa                                                                                        >  7:228714/1‑131 (MQ=255)                                                                           acGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGATTTCAGTGATAAAGGGCATAg                                                                   >  8:64675/1‑149 (MQ=255)                                                                             gTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGATTTCAGTg                                                                                >  4:90560/1‑134 (MQ=255)                                                                             gTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGATTTCAGTg                                                                                <  3:90560/134‑1 (MQ=255)                                                                              tttGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGATTTCAGTGAAAAAGGGCATAGCTg                                                                >  8:132307/1‑149 (MQ=255)                                                                                 gcgcAGTGCCTGTTCGGCACCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGATTTCAGTGATAAAg                                                                          >  6:333759/1‑136 (MQ=255)                                                                                 gcgcAGTGCCTGTTCGGCACCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGATTTCAGTGATAAAg                                                                          <  5:333759/136‑1 (MQ=255)                                                                                   gcAGTGCCTGTTCTGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGATTTCAGTGATAAAGGGCATAGCTGGTCCt                                                           >  5:13651/1‑149 (MQ=255)                                                                                    cAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGATTTCAGTGATAAAGGGCATAGCTGGTCCTc                                                          <  4:238318/149‑1 (MQ=255)                                                                                    cAGTGCCTGTTCGGCACCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGATTTCAGTGATAAAGGGCATAGCTGGTCCt                                                           >  8:137177/1‑148 (MQ=255)                                                                                     aGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGATTTCAGTGATAAAGGGCATAGCTGGTCCt                                                           >  5:305974/1‑147 (MQ=255)                                                                                     aGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGATTTCAGTGATAAAGGGCATAGCTGGTCCTCa                                                         <  7:64675/149‑1 (MQ=255)                                                                                     aGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGATTTCAGTGATAAAGGGCATAGCTGGTCCTCa                                                         <  5:201062/149‑1 (MQ=255)                                                                                      gTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGATTTCAGTGATAAAGGGCATAGCTGGTCCTCAg                                                        <  6:13651/149‑1 (MQ=255)                                                                                       tGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCTAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGATTTCAGTGATAAAGGGCATAGCTGGTCCTCAgg                                                       <  7:74712/149‑1 (MQ=255)                                                                                       tGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGATTTCAGTGATAAAGGGCATAGCTGGTCCTCAgg                                                       <  6:353711/149‑1 (MQ=255)                                                                                       tGCCTGTTCGGCACCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGATTTCAGTGATAAAGGGCATAGCTGGTCCTCAgg                                                       <  7:137177/149‑1 (MQ=255)                                                                                           tGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCTAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGATTTCa                                                                                   >  4:274948/1‑117 (MQ=255)                                                                                           tGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCTAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGATTTCa                                                                                   <  3:274948/117‑1 (MQ=255)                                                                                           tGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGATTTCAGTGATAAAGGGCATAGATGGTCCTCAGGGTa                                                    >  6:4181/1‑148 (MQ=255)                                                                                                 gcgccgggcgccggGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGATTTc                                                                                    <  1:150911/110‑1 (MQ=255)                                                                                                 gcgccgggcgccggGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGATTTc                                                                                    >  2:150911/1‑110 (MQ=255)                                                                                                  cgccgggcgccgggTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGATTTCAGTGATAAAGGGCATAGCTGGTCCTCAGGGTAAAACACAg                                            <  1:313284/149‑1 (MQ=255)                                                                                                         cgccgggTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGATTTCAGTGATAAAGGGCATAGCTGGTCCTCAGGGTAAAACACAGTAATCAg                                     <  5:4181/149‑1 (MQ=255)                                                                                                          gccgggTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGATTTCAGTGATAAAGGGCATAGCTGGTCCTCAGGGTAAAACACAGTAATCAgg                                    <  4:187410/149‑1 (MQ=255)                                                                                                          gccgggTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGATTTCAGTGATAAAGGGCATAGCTGGTCCTCAGGGTAAAACACAGTAATCAgg                                    <  2:352630/149‑1 (MQ=255)                                                                                                                 cccAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGATTTCAGTGAAAAAGGGCATAGCTGGTCCTCAGGGTAAAACACAGTAATCAGGGTTCATg                             <  7:132307/149‑1 (MQ=255)                                                                                                                    aGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGATTTCAGTGATAAAGGGCATAGCTGGTCCTCAGGGTAAAACACAGTAATCAGGGTTCATGGc                           >  8:135222/1‑148 (MQ=255)                                                                                                                      gCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGATTTCAGTGATAAAGGGCATAGCTGGTCCTCAGGGTAAAAcaca                                             <  7:119673/128‑1 (MQ=255)                                                                                                                      gCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGATTTCAGTGATAAAGGGCATAGCTGGTCCTCAGGGTAAAAcaca                                             >  8:119673/1‑128 (MQ=255)                                                                                                                              aTCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGATTTCAGTGATAAAGGGCATAGCTGGTCCTCAGGGTAAAACACAGTAATCAGGGTGCATGGCTACCCCTGTgg                >  4:77048/1‑149 (MQ=255)                                                                                                                              aTCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGATGTCAGTGATAAAGGGCATAGCGGGTCCTCAGGGTAAAACACAGTAATCAGGGTTCCTGGCTAACCCTgggg                >  4:72032/1‑149 (MQ=255)                                                                                                                              aTCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTGTTGATTTCAGTGATAAAGGGCATAGCTGGTCCTCAGGGTAAAACACAGTAATCAGGGTTCATGGCTAACCCTGTgg                <  6:305974/149‑1 (MQ=255)                                                                                                                                   ggCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGATTTCAGTGATAAAGGGCATAGCTGGTCCTCAGGGTAAAACACAGTAATCAGGGTTCATGGCTAACCCTGTGGGAGCg           <  2:182000/149‑1 (MQ=255)                                                                                                                                     ccATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGATTTCAGTGATAAAGGGCATAGCTGGTCCTCAGGGTAAAACACAGTAATCAGGGTTCATGGCTAACCCTGTGGGAGCggg         >  5:161708/1‑149 (MQ=255)                                                                                                                                      cATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCGGAGCGAAGGTTTTGATTTCAGTGATAAAGGGCATAGCTGGTCCTCAGGGTAAAACACAGTAATCAGGGTTCATGGCTAACCCTGTGGGAGCgg          >  1:98035/1‑149 (MQ=255)                                                                                                                                      cATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGATTTCAGTGATAAAGGGCATAGCTGGTCCTCAGGGTAAAACACAGTAATCAGGGTTCATGGCTAACCCTGTGGGAGCggg         >  8:191730/1‑148 (MQ=255)                                                                                                                                      cATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGCTTTTGATTTCAGTGATAAAGGGCATAGCTGGTCCTCAGGGTAAAACACAGTAATCAGGGTTCATGGCTAACCCTGTGGGAGCGGGc        <  7:191730/149‑1 (MQ=255)                                                                                                                                           gCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGATTTCAGTGATAAAGGGCATAGCTGGTCCTCAGGGTAAAACACAGTAATCAGGGTTCATGGCTAACCCTGTGGGAGCGGGCATGcc   >  2:275955/1‑149 (MQ=255)                                                                                                                                            ccAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGATTTCAGTGATAAAGGGCATAGCTGGTCCTCAGGGTAAAACACAGTAATCAGGGTTCATGGCTAACCCTGTGGGAGCGGGCATGccc  >  6:238722/1‑149 (MQ=255)                                                                                                                                             |                                                                                                                                                      ACGAACTTCAGGCGGTCTTGCGATGCGCCAGGGGCCAGGCCTTGTTTTTCCAGTGCCGGCCAGGCGTTGGCGACACGTTTGCGCAGTGCCTGTTCGGCGCCGGGCGCCGGGTCCCAGGCCACCACATCCAGGCCATGGGCCAGGGCGCGGGCGACCCAGCCGCTGCCGATCACGCCGCTACCCAGGGCG‑‑GCGAAGGTTTTGATTTCAGTGATAAAGGGCATAGCTGGTCCTCAGGGTAAAACACAGTAATCAGGGTTCATGGCTAACCCTGTGGGAGCGGGCATGCCC  >  NC_002947/363262‑363549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑