Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,896,399 T→C 10.0% intergenic (+32/‑176) PP_4304 → / → sbp‑I VIC family cation transporter/sulfate ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,896,3990TC10.0% 135.5 / 5.6 50intergenic (+32/‑176)PP_4304/sbp‑IVIC family cation transporter/sulfate ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (24/21);  new base C (3/2);  total (27/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.98e-01

AATGCCATGCGTGGGGAGCAGCTGCAGCATGATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCC  >  NC_002947/4896251‑4896546
                                                                                                                                                    |                                                                                                                                                      
aaTGCCATGCGTGGGGAGCAGCTGCAGCATGATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAAt                                                                                                                                                        <  8:346174/149‑1 (MQ=255)
    ccATGCGTGGGGAGCAGCTGCAGCATGATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGc                                                                                                                                                    <  8:150/149‑1 (MQ=255)
     cATGCGTGGGGAGCAGCTGCAGCATGATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCt                                                                                                                                                   >  6:60809/1‑149 (MQ=255)
      aTGCGTGGGGAGCAGCTGCAGCATGATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCATCTTCGCGGGTAAATCCGCTc                                                                                                                                                  >  1:130921/1‑149 (MQ=255)
           tGGGGAGCAGCTGCAGCATGATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTa                                                                                                                                               >  8:137442/1‑147 (MQ=255)
            ggggAGCAGCTGCAGCATGATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAACCCGCTCCTAcgg                                                                                                                                            >  2:327070/1‑147 (MQ=255)
                agcagcTGCAGCATGATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGgcgc                                                                                                                                        <  4:245987/149‑1 (MQ=255)
                 gcagcTGCAGCATGATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGcgcg                                                                                                                                       >  1:60713/1‑149 (MQ=255)
                    gcTGCAGCATGATTGCCCGACCTGCCACAAGAAGCCCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCGCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGAc                                                                                                                                    >  7:166882/1‑149 (MQ=255)
                       gcagcaTGATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCgc                                                                                                                                 >  5:107315/1‑148 (MQ=255)
                               aTTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTc                                                                                                                         <  7:274987/149‑1 (MQ=255)
                                ttGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTcc                                                                                                                        >  8:23453/1‑149 (MQ=255)
                                  gCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGCGCACGGTccg                                                                                                                       <  6:107315/148‑2 (MQ=255)
                                         cTGCCACAAGAAGACACACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGa                                                                                                            <  8:106777/149‑1 (MQ=255)
                                            ccACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAg                                                                                                           <  5:60809/147‑1 (MQ=255)
                                            ccACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGc                                                                                                          <  5:155501/148‑1 (MQ=255)
                                            ccACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGc                                                                                                          <  1:278228/148‑1 (MQ=255)
                                             cacaAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCgg                                                                                                        >  1:69714/1‑149 (MQ=255)
                                             cacaAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCATCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCgg                                                                                                        <  2:130921/149‑1 (MQ=255)
                                              acaAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCATGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTc                                                                                                                         >  3:234538/1‑134 (MQ=255)
                                                 agcagaCCCACGAGCAGGGGGGGGCGTTCTGTTCTCGCTGGGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGttt                                                                                                    <  4:261988/146‑1 (MQ=255)
                                                 agaagaCCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGttt                                                                                                    <  2:69714/149‑1 (MQ=255)
                                                  gaagaCCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGTGGGTAAACCCGCTCCTACGGG‑‑‑GGACCGCGCACGGTCCGCGCCTGTAGGAGCGGATTTa                                                                                                   <  5:323825/149‑1 (MQ=17)
                                                  gaagaCCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTa                                                                                                   >  6:171315/1‑149 (MQ=255)
                                                   aagaCCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTAc                                                                                                  >  7:105112/1‑149 (MQ=255)
                                                     gaCCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTAccc                                                                                                <  7:137442/149‑1 (MQ=255)
                                                        ccACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGttt                                                                                                    >  7:291850/1‑142 (MQ=255)
                                                        ccACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGttt                                                                                                    <  8:291850/142‑1 (MQ=255)
                                                          aCGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGTGCGGGGTTTCCcgcg                                                                                             >  3:246457/1‑147 (MQ=255)
                                                                     gcggcgTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCCTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGca                                                                                 >  4:290029/1‑148 (MQ=255)
                                                                                ttctCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGGGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGaa                                                                        <  8:105112/146‑1 (MQ=255)
                                                                                ttctCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCAAATAGCCGGTAAATAAGCAACAACAGGCGCGGACCCTGGCCGGTCC‑‑‑CCCCTAGGAGCGGGTTTTACCCCGAAGACGCCCGCACAGGCACAAGc                                                                      >  4:312300/1‑148 (MQ=255)
                                                                                     gCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGGACAGGCAACAGCATAAc                                                                 >  4:213505/1‑148 (MQ=255)
                                                                                         tgGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCaaa                                                             <  2:60713/147‑1 (MQ=255)
                                                                                            gAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCaaa                                                             >  6:272418/1‑145 (MQ=255)
                                                                                            gAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCaaa                                                             <  5:272418/145‑1 (MQ=255)
                                                                                             aaTGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCATAGCGGGTAAATACGCACCGACAGGCGCGGCCCGGGGGCGGTCC‑‑‑CCCGTAGGCGCGGGGTTACCCGCGAAGAGGCCGGCCCACGCAGAAGCCTaaccaaaccaat                                                        >  6:326245/1‑147 (MQ=255)
                                                                                                     gttTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGtt                                                 <  3:290029/148‑1 (MQ=255)
                                                                                                     gttTCCAAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGTTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGGGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGtt                                                 <  3:312300/148‑1 (MQ=255)
                                                                                                          ccAACGCGTGATCGCGCCTGCGCCGGCCTCTTCGCTGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTc                                                <  7:142157/144‑1 (MQ=255)
                                                                                                           cAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATACGCTCCTACAGGCGCGGCCCGTGCGCGGTCC‑‑‑CCCGGAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCAGAACCCAAGCATTTTTTGTTCTGTAAc                                          >  8:346312/1‑149 (MQ=38)
                                                                                                                    aTAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGGTTACCCGCGAAGAGGCCGGCCCAGGCAGAAGCAGAACCAAAGCATTTTTTGTTGTTTAACCCGTATtaa                                 >  1:109140/1‑149 (MQ=38)
                                                                                                                                      tctTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGc               >  2:165898/1‑149 (MQ=255)
                                                                                                                                       ctTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCCCCCGTTATAGTCGCt              >  7:162621/1‑149 (MQ=255)
                                                                                                                                        ttCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTg             >  3:88052/1‑149 (MQ=255)
                                                                                                                                         tCGCGGGTAAACCCGCTCCTACGGG‑‑‑GGACCGCGCACGGTCCGCGCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTgg            >  5:210295/1‑149 (MQ=17)
                                                                                                                                           gcgGGTAAACCCGCTCCTACGGG‑‑‑GGACCGCGCACGGTCCGCGCCTGTAGGAGCGGATTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTgg            >  7:330051/1‑147 (MQ=17)
                                                                                                                                           gcgGGTAAACCCGCTCCTACGGG‑‑‑GGACCGCGCACGGTCCGCGCCTGTAGGAGCGGATTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTgg            <  8:330051/147‑1 (MQ=17)
                                                                                                                                                  aaTCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGcc   <  7:346312/149‑1 (MQ=255)
                                                                                                                                                  aaTCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGcc   >  4:50773/1‑149 (MQ=255)
                                                                                                                                                   aTCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGccc  <  5:326245/149‑1 (MQ=255)
                                                                                                                                                    tCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCGGGCAATCTGccc  >  5:109296/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
AATGCCATGCGTGGGGAGCAGCTGCAGCATGATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCC  >  NC_002947/4896251‑4896546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: