| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 382,714 | T→C | 10.6% | intergenic (‑170/‑97) | PP_0317 ← / → PP_0318 | methyl‑accepting chemotaxis transducer/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 382,714 | 0 | T | C | 10.6% | 121.3 / 4.6 | 48 | intergenic (‑170/‑97) | PP_0317/PP_0318 | methyl‑accepting chemotaxis transducer/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (20/22); new base C (2/3); total (22/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01 | |||||||||||
TGGGAAAAGAGCGACAAAATATGACTTGCCGGGCTATCGGTCGTGACTGGCCAGTCTTGAATGCGTCCGGGGAAAATGGTGCGACCGCGAACCTTTTCCTGACCGGCGGTTGATCCAGGTCGGAACAAGGGGTCGATTACCCTGTCAATCTGCGGGTTCGACACCTTATGAATC‑CGATATTTAATGGCGATAGGGCGCACTTTTCTTTATCGTGTGTGCCCCTCGCAACAACCTGAGATAGACCCATGCTGGAAGCTTCCCTGAATCAAATCGAGCAACTGGTCAGCGACCTGATG > NC_002947/382566‑382861 | tGGGAAAAGAGCGACAAAATATGACTTGCCGGGCTATCGGTCGTGACTGGCCAGTCTTGAATGCGTCCGGGGAAAATGGTGCGACCGCGAACCTTTTCCTGACCGGCGGTTGATCCAGGTCGGAACAAGGGGTCGATTACCCTGTCAAt < 1:355995/149‑1 (MQ=255) gAAAAGAGCGACAAAATATGACTTGCCGGGCTATCGGTCGTGACTGGCCAGTCTTGAATGCGTCCGGGGAAAATGGTGCGACCGCGAACCTTTTCCTGACCGGCGGTTGATCCAGGTCGGAACAAGGGGTCGATTACCCTGTCAATCTg > 6:259508/1‑149 (MQ=255) aaaGAGCGACAAAATATGACTTGCCGGGCTATCGGTCGTGACTGGCCAGTCTTGAATGCGTCCGGGGAAAATGGTGCGACCGCGAACCTTTTCCTGACCGGCGGTTGATCCAGGTCGGAACAAGGGGTCGATTACCCTGTCAATCTGCg < 7:313153/149‑1 (MQ=255) aaaTATGACTTGCCGGGCTATCGGTCGTGACTGGCCAGTCTTGAATGCGTCCGGGGAAAATGGTGCGACCGCGAACCTTTTCCTGACCGGCGGTTGATCCAGGTCGGAACAAGGGGTCGATTACCCTGTCAATCTGCGGGTTCGACAcc < 6:150141/149‑1 (MQ=255) tGACTTGCCGGGCTATCGGTCGTGACTGGCCAGTCTTGAATGCGTCCGGGGAAAATGGTGCGACCGCGAACCTTTTCCTGACCGGCGGTTGATCCAGGTCGGAACAAGGGGTCGATTACCCTGTCAATCTGCGGGTTCGACACCTTAt < 8:339358/148‑1 (MQ=255) gCCGGGCTATCGGTCGTGACTGGCCAGTCTTGAATGCGTCCGGGGAAAATGGTGCGACCGCGAACCTTTTCCTGACCGGCGGTTGATCCAGGTCGGAACAAGGGGTCGATTACCCTGTCAATCTGCGGGTTCGACACCTTATGAATc < 3:132657/147‑1 (MQ=255) gCCGGGCTATCGGTCGTGACTGGCCAGTCTTGAATGCGTCCGGGGAAAATGGTGCGACCGCGAACCTTTTCCTGACCGGCGGTTGATCCAGGTCGGAACAAGGGGTCGATTACCCTGTCAACCTGCGGGTTCGACACCTTATGAATc‑c > 7:172758/1‑148 (MQ=255) ccGGGCTATCGGTCGTGACTGGCCAGTCTTGAATGCGTCCGGGGAAAATGGTGCGACCGCGAACCTTTTCCTGACCGGCGGTTGATCCAGGTCGGAACAAGGGGTCGATTACCCTGTCAATCTGCGGGTTCGACACCTTATGAATC‑CGa < 6:293794/149‑1 (MQ=255) ccGGGCTATCGGTCGTGACTGGCCAGTCTTGAATGCGTCCGGGGAAAATGGTGCGACCGCGAACCTTTTCCTGACCGGCGGTTGATCCAGGTCGGAACAAGGGGTCGATTACCCTGTCAATCTGCGGGTTCGACACCTTATGAATC‑CGa > 3:156124/1‑149 (MQ=255) tATCGGTCGTGACTGGCCAGTCTTGAATGCGTCCGGGGAAAATGGTGCGACCGCGAACCTTTTCCTGACCGGCGGTTGATCCAGGTCGGAACAAGGGGTCGATTACCCTGTCAATCTGCGGGTTCGACACATTATGAATC‑CGATAttt > 5:51270/1‑148 (MQ=255) ggTCGTGACTGGCCAGTCTTGAATGCGTCCGGGGAAAATGGTGCGACCGCGAACCTTTTCCTGACCGGCGGTTGATCCAGGTCGGAACAAGGGGTCGATTACCCTGTCAACCTGCGGGTTCGACACCTTATGAATC‑CGATATTTAATg < 3:157325/148‑1 (MQ=255) gTGACTGGCCAGTCTTGAATGCGTCCGGGGAAAATGGTGCGACCGCGAACCTTTTCCTGACCGGCGGTTGATCCAGGTCGGAACAAGGGGTCGATTACCCTGTCAACCTGCGGGTTCGACACCTTATGAATC‑CGATATTTAATGGCGa > 2:157829/1‑148 (MQ=255) gCCAGTCTTGAATGCGTCCGGGGAAAATGGTGCGACCGCGAACCTTTTCCTGACCGGCGGTTGATCCAGGTCGGAACAAGGGGTCGATTACCCTGTCAACCTGCGGGTTCGACACCTTATGAATC‑CGATATTTAATGGCGATAGGgc < 1:157829/147‑1 (MQ=255) aGTCTTGAATGCGTCCGGGGAAAATGGTGCGACCGCGAACCTTTTCCTGACCGGCGGTTGATCCAGGTCGGAACAAGGGGTCGATTACCCTGTCAATCTGCGGGTTCGACACCTTAt < 4:124228/117‑1 (MQ=255) aGTCTTGAATGCGTCCGGGGAAAATGGTGCGACCGCGAACCTTTTCCTGACCGGCGGTTGATCCAGGTCGGAACAAGGGGTCGATTACCCTGTCAATCTGCGGGTTCGACACCTTAt > 3:124228/1‑117 (MQ=255) cTTGAATGCGTGCGGGGAAAATGGTGCGACCGCGAAGCTTTTCCTGACGGGCGGTTGATCCAGGTCGGAACAAGGGGTCGATTACCCTGTCAATCTGCGGGTTCGACACCTTATGAATC‑CGATATTTAATGGCGATAGGGCGCACttt < 6:51270/148‑1 (MQ=255) ttGAATGCGTCCGGGGAAAATGGTGCGACCGCGAACCTTTTCCTGACCGGCGGTTGATCCAGGTCGGAACAAGGGGTCGATTACCCTGTCAATCTGCGGGTTCGACACCTTATGAATC‑CGATATTTAATGGCGATAGGGCGCACTTTTc > 2:5615/1‑149 (MQ=255) ttGAATGCGTCCGGGGAAAATGGTGCGACCGCGAACCTTTTCCTGACCGGCGGTTGATCCAGGTCGGAACAAGGGGTCGATTACCCTGTCAATCTGCGGGTTCGACACCTTATGAATC‑CGATATTTAATGGCGATAGGGCGCACTTTTc > 6:153859/1‑149 (MQ=255) gAATGCGTCCGGGGAAAATGGTGCGACCGCGAACCTTTTCCTGACCGGCGGTTGATCCAGGTCGGAACAAGGGGTCGATTACCCTGTCAATCTGCGGGTTCGACACCTTATGAATC‑CGATATTTAATGGCGATAGGGCGCACtttt < 4:156124/146‑1 (MQ=255) cGGGGAAAATGGTGCGACCGCGAACCTTTTCCTGACCGGCGGTTGATCCAGGTCGGAACAAGGGGTCGATTACCCTGTCAATCTGCGGGTTCGACACCTTAt < 8:289812/102‑1 (MQ=255) cGGGGAAAATGGTGCGACCGCGAACCTTTTCCTGACCGGCGGTTGATCCAGGTCGGAACAAGGGGTCGATTACCCTGTCAATCTGCGGGCTCGACACCTTATGAATC‑CGATATTTAATGGCGATAGGGCGCACTTTTCTTTATCgtgt < 5:259508/148‑1 (MQ=255) ggAAAATGGTGCGACCGCGAACCTTTTCCTGACCGGCGGTTGATCCAGGTCGGAACAAGGGGTCGATTACCCTGTCAATCTGCGGGTTCGACACCTTATGAATC‑CGATATTTAATGGCGATAGGGCGCACTTTTCTTTATCGTGTGTGc > 6:169764/1‑149 (MQ=255) gAAAATGGTGCGACCGCGAACCTTTTCCTGACCGGCGGTTGATCCAGGTCGGAACAAGGGGTCGATTACCCTGTCAATCTGCGGGTTCGACACCTTATGAATC‑CGATATTTAATGGCGATAGGGCGCACTTTTCTTTATCGTGTGTGcc > 1:330845/1‑149 (MQ=255) tGCGACCGCGAACCTTTTCCTGACCGGCGGTTGATCCAGGTCGGAACAAGGGGTCGATTACCCTGTCAATCTGCGGGTTCGACACCTTATGAATC‑CGATATTTAATGGCGATAGGGCGCACTTTTCTTTATCGTGTGTGCCCCTCGcaa > 2:29155/1‑149 (MQ=255) tGCGACCGCGAACCTTTTCCTGACCGGCGGTTGATCCAGGTCGGAACAAGGGGTCGATTACCCTGTCAATCTGCGGGTTCGACACCTTATGAATC‑CGATATTTAATGGCGATAGGGCGCACTTTTCTTTATCGTGTGTGCCCCTCGcaa > 2:11068/1‑149 (MQ=255) aCCGCGAACCTTTTCCTGACCGGCGGTTGATCCAGGTCGGAACAAGGGGTCGATTACCCTGTCAATCTGCGGGTTCGACACCTTATGAATC‑CGATATTTAATGGCGATAGGGCGCACTTTTCTTTATCGTGTGTGCCCCTCGCaacaac < 5:153859/149‑1 (MQ=255) gAACCGTGTCCTGACAGGCGGTTGCTGCAGGTCGGTACACGGGTTCGAGTACCCTGGCAATCTGCGGGTTCGACACCTTATGAATC‑CGATATTTAATGGCGATAGGGCGCACTTTTCTTTCTCGTGTGTGCCCCTCGCAACAACCTgag < 8:75409/149‑1 (MQ=255) cTTTTCCTGACCGGCGGTTGATCCAGGTCGGAACAAGGGGTCGATTACCCTGTCAATCTGCGGGTTCGACACCTTATGAATC‑CGATATTTAATGGCGATAGGGCGCACTTTTCTTTAt > 6:282461/1‑118 (MQ=255) ttCCTGACCGGCGGTTGATCCAGGTCGGAACAAGGGGTCGATTACCCTGTCAATCTGCGGGTTCGACACCTTATGAATC‑CGATATTTAAt > 1:89716/1‑90 (MQ=255) ttCCTGACCGGCGGTTGATCCAGGTCGGAACAAGGGGTCGATTACCCTGTCAATCTGCGGGTTCGACACCTTATGAATC‑CGATATTTAAt < 2:89716/90‑1 (MQ=255) tGACCGGCGGTTGATCCAGGTCGGAACAAGGGGTCGATTACCCTGTCAATCTGCGGGTTCGACACCTTATGAATC‑CGATATTTAATGGCGATAGGGCGCACTTTTCTTTATCGTGTGTGCCCCTCGCAACAACCTGAGATAGACCCATg > 7:293330/1‑149 (MQ=255) tGACCGGCGGTTGATCCAGGTCGGAACAAGGGGTCGATTACCCTGTCAATCTGCGGGTTCGACACCTTATGAATC‑CGATATTTAATGGCGATAGGGCGCACTTTTCTTTATCGTGTGTGCCCCTCGCAACAACCTGAGATAGACCCATg > 6:340988/1‑149 (MQ=255) gACCGGCGGTTGATCCAGGTCGGAACAAGGGGTCGATTACCCTGTCAATCTGCGGGTTCGACACCTTATGAATC‑CGATATTTAATGGAGATAGGGCGCACTTTTCTTAATAGTGTGTGCCCCTCGCAACAACCTGAGATAGACCCATga > 6:210044/1‑148 (MQ=255) aCCGGCGGTTGATCCAGGTCGGAACAAGGGGTCGATTACCCTGTCAATCTGCGGGTTCGACACCTTATGAATC‑Cg < 3:274426/75‑1 (MQ=255) aCCGGCGGTTGATCCACGACGGAACAACGGGTCGATTACCCTGTCAATATGCGGGTTCGACACCTTATGAATcag > 4:274426/1‑73 (MQ=255) ggcggTTGATGCAGGTCGGAACAAGGGGTCGATTACCCTGTCAATCTGCGGGTTCGACAACTTATGAATC‑CGATATTTAATGGCGATAGGGCGCACTTTTCTTTATCGTGTGTGCCCCTCGCAACAACCTGAGATAGACCCATGCTGGa < 5:210044/149‑1 (MQ=255) ggcggTTGATCCAGGTCGGAACAAGGGGTCGATTACCCTGTCAATCTGCGGGTTCGACACCTTATGAATC‑CGATATTTAATGGCGATAGGGCGCACTTTTCTTTATCGTGTGTGCCCCTCGCAACAACCTGAGATAGACCCATGCTGGa < 1:29155/149‑1 (MQ=255) tGATCCAGGTCGGAACAAGGGGTCGATTACCCTGTCAATCTGCGGGTTCGACACCTTATGAATC‑CGATATTTAATGGCGATAGGGCGCACTTTTCTTTATCGTGTGTGCCCCTCGCAACAACCTGAGATAGACCCATg > 5:323662/1‑138 (MQ=255) tGATCCAGGTCGGAACAAGGGGTCGATTACCCTGTCAATCTGCGGGTTCGACACCTTATCAATC‑CGATATTTAATGGCGATAGGGCGCACTTTTCTTTATCGTGTGTGCCCCTCGCAACAACCTGAGATAGACCCATg < 6:323662/138‑1 (MQ=255) tCCAGGTCGGAACAAGGGGTCGATTACCCTGTCAATCTGCGGGTTCGACACCTTATGAATC‑CGATATTTAATGGCGATAGGGCGCACTTTTCTTTATCGTGTGTGCCCCTCGCAACAACCTGAGATAGACCCATGCTGGAAGCTTCCCt > 8:204831/1‑149 (MQ=255) ggTCGGAACAAGGGGTCGATTACCCTGTCAATCTGCGGGTTCGACACCTTATGAATC‑CGATATTTAATGGCGATAGGGCGCACTTTTCTTTATCGTGTGTGCCCCTCGCAACAACCTGAGATAGACCCATGCTGGAAGCTTCCCTGAAt < 5:169764/149‑1 (MQ=255) ccagcgctCGAATACCCTGTCAAGCTGCGGGATCGACACCATATGAATC‑CGATATTTAATGGCGATAGGGCGCACTTTTCTTTATCGTGTGTGCCCCTCGCAACAACCTGAGATAGACCCATGCTGGAAGCTTCCCTGAATCAAATCGa < 8:293330/142‑1 (MQ=255) ggTCGATTACCCTGTCAATCTGCGGGTTCGACACCTTATGAATC‑CGATATTTAATGGCGATAGGGCGCACTTTTCTTTATCGTGTGTGCCCCTCGCAACAACCTGAGATAGACCCATGCTGGAAGCTTCCCTGAATCAAATCGAGCAAc < 1:5615/149‑1 (MQ=255) cccTGTCAACCTGCGGGTTCGACACCTTATGAATC‑CGATATTTAATGGCGATAGGGCGCACTTTTCTTTATCGTGTGTGCCCCTCGCAACAACCTGAGATAGACCCATGCTGGAAGCTTCCCTGAATCAAATCGAGCAACTGGTCAGc < 8:172758/148‑1 (MQ=255) ccTGTCAATCTGCGGGTTCGACACCTTATGAATCTCGATATTTAATGGCGATAGGGCGCACTTTTCTTTATCGTGTGTGCCCCTCGCAACAACCTGAGATAGACCCATGCTGGAAGCTTCCCTGAATCAAATCGAGCAACTGGTCAGc > 1:230911/1‑148 (MQ=255) ccTGTCAATCTGCGGGTTCGACACCTTATGAATC‑CGATATTTAATGGCGATAGGGCGCACTTTTCTTTATCGTGTGTGCCCCTCGCAACAACCTGAGATAGACCCATGCTGGAAGCTTCCCTGAATCAAATCGAGCAACTGGTCAGCGa > 2:108240/1‑149 (MQ=255) aaTCTGCGGGTTCGACACCTTATGAATC‑CGATATTTAATGGCGATAGGGCGCTCTTTTCTTTATCGTGTGTGCCCCTCGCAACAACCTGAGATAGACCCATGCTGGAAGCTTCCCTGAATCAAATCGAGCAACTGGTCAGCGACCTGa < 2:330845/148‑1 (MQ=255) aTCTGCGGGTTCGACACCTTATGAATC‑CGATATTTAATGGCGATAGGGCGCACTTTTCTTTATCGTGTGTGCCCCTCGCAACAACCTGAGATAGACCCATGCTGGAAGCTTCCCTGAATCAAATCGAGCAACTGGTCAGCGACCTGATg < 5:340988/149‑1 (MQ=255) aTCTGCGGGTTCGACACCTTATGAATC‑CGATATTTAATGGCGATAGGGCGCACTTTTCTTTATCGTGTGTGCCCCTCGCAACAACCTGAGATAGACCCATGCTGGAAGCTTCCCTGAATCAAATCGAGCAACTGGTCAGCGACCTGATg < 3:105395/149‑1 (MQ=255) | TGGGAAAAGAGCGACAAAATATGACTTGCCGGGCTATCGGTCGTGACTGGCCAGTCTTGAATGCGTCCGGGGAAAATGGTGCGACCGCGAACCTTTTCCTGACCGGCGGTTGATCCAGGTCGGAACAAGGGGTCGATTACCCTGTCAATCTGCGGGTTCGACACCTTATGAATC‑CGATATTTAATGGCGATAGGGCGCACTTTTCTTTATCGTGTGTGCCCCTCGCAACAACCTGAGATAGACCCATGCTGGAAGCTTCCCTGAATCAAATCGAGCAACTGGTCAGCGACCTGATG > NC_002947/382566‑382861 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |