Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,896,422	T→C	9.9%	intergenic (+55/‑153)	PP_4304 → / → sbp‑I	VIC family cation transporter/sulfate ABC transporter substrate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,896,422	0	T	C	9.9%	135.2 / 5.7	52	intergenic (+55/‑153)	PP_4304/sbp‑I	VIC family cation transporter/sulfate ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (24/21);  new base C (2/3);  total (27/25)
Fisher's exact test for biased strand distribution p-value = 6.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.37e-01

ATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACACCCTTCAAGAACAAG  >  NC_002947/4896282‑4896566                                                                                                                                             |                                                                                                                                                    aTTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTc                                                                                                                                             <  7:274987/149‑1 (MQ=255)  ttGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTcc                                                                                                                                            >  8:23453/1‑149 (MQ=255)    gCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGCGCACGGTccg                                                                                                                                           <  6:107315/148‑2 (MQ=255)           cTGCCACAAGAAGACACACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGa                                                                                                                                <  8:106777/149‑1 (MQ=255)              ccACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAg                                                                                                                               <  5:60809/147‑1 (MQ=255)              ccACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGc                                                                                                                              <  1:278228/148‑1 (MQ=255)              ccACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGc                                                                                                                              <  5:155501/148‑1 (MQ=255)               cacaAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCgg                                                                                                                            >  1:69714/1‑149 (MQ=255)               cacaAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCATCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCgg                                                                                                                            <  2:130921/149‑1 (MQ=255)                acaAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCATGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTc                                                                                                                                             >  3:234538/1‑134 (MQ=255)                   agcagaCCCACGAGCAGGGGGGGGCGTTCTGTTCTCGCTGGGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGttt                                                                                                                        <  4:261988/146‑1 (MQ=255)                   agaagaCCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGttt                                                                                                                        <  2:69714/149‑1 (MQ=255)                    gaagaCCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGTGGGTAAACCCGCTCCTACGGG‑‑‑GGACCGCGCACGGTCCGCGCCTGTAGGAGCGGATTTa                                                                                                                       <  5:323825/149‑1 (MQ=17)                    gaagaCCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTa                                                                                                                       >  6:171315/1‑149 (MQ=255)                     aagaCCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTAc                                                                                                                      >  7:105112/1‑149 (MQ=255)                       gaCCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTAccc                                                                                                                    <  7:137442/149‑1 (MQ=255)                          ccACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGttt                                                                                                                        <  8:291850/142‑1 (MQ=255)                          ccACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGttt                                                                                                                        >  7:291850/1‑142 (MQ=255)                            aCGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGTGCGGGGTTTCCcgcg                                                                                                                 >  3:246457/1‑147 (MQ=255)                                       gcggcgTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCCTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGca                                                                                                     >  4:290029/1‑148 (MQ=255)                                                  ttctCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGGGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGaa                                                                                            <  8:105112/146‑1 (MQ=255)                                                  ttctCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCAAATAGCCGGTAAATAAGCAACAACAGGCGCGGACCCTGGCCGGTCC‑‑‑CCCCTAGGAGCGGGTTTTACCCCGAAGACGCCCGCACAGGCACAAGc                                                                                          >  4:312300/1‑148 (MQ=255)                                                       gCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGGACAGGCAACAGCATAAc                                                                                     >  4:213505/1‑148 (MQ=255)                                                           tgGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGG‑TTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCaaa                                                                                 <  2:60713/147‑1 (MQ=255)                                                              gAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCaaa                                                                                 <  5:272418/145‑1 (MQ=255)                                                              gAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCaaa                                                                                 >  6:272418/1‑145 (MQ=255)                                                               aaTGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCATAGCGGGTAAATACGCACCGACAGGCGCGGCCCGGGGGCGGTCC‑‑‑CCCGTAGGCGCGGGGTTACCCGCGAAGAGGCCGGCCCACGCAGAAGCCTaaccaaaccaat                                                                            >  6:326245/1‑147 (MQ=255)                                                                       gttTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGtt                                                                     <  3:290029/148‑1 (MQ=255)                                                                       gttTCCAAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGTTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGGGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGtt                                                                     <  3:312300/148‑1 (MQ=255)                                                                            ccAACGCGTGATCGCGCCTGCGCCGGCCTCTTCGCTGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTc                                                                    <  7:142157/144‑1 (MQ=255)                                                                             cAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATACGCTCCTACAGGCGCGGCCCGTGCGCGGTCC‑‑‑CCCGGAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCAGAACCCAAGCATTTTTTGTTCTGTAAc                                                              >  8:346312/1‑149 (MQ=38)                                                                                      aTAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGGTTACCCGCGAAGAGGCCGGCCCAGGCAGAAGCAGAACCAAAGCATTTTTTGTTGTTTAACCCGTATtaa                                                     >  1:109140/1‑149 (MQ=38)                                                                                                        tctTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGc                                   >  2:165898/1‑149 (MQ=255)                                                                                                         ctTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCCCCCGTTATAGTCGCt                                  >  7:162621/1‑149 (MQ=255)                                                                                                          ttCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTg                                 >  3:88052/1‑149 (MQ=255)                                                                                                           tCGCGGGTAAACCCGCTCCTACGGG‑‑‑GGACCGCGCACGGTCCGCGCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTgg                                >  5:210295/1‑149 (MQ=17)                                                                                                             gcgGGTAAACCCGCTCCTACGGG‑‑‑GGACCGCGCACGGTCCGCGCCTGTAGGAGCGGATTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTgg                                <  8:330051/147‑1 (MQ=17)                                                                                                             gcgGGTAAACCCGCTCCTACGGG‑‑‑GGACCGCGCACGGTCCGCGCCTGTAGGAGCGGATTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTgg                                >  7:330051/1‑147 (MQ=17)                                                                                                                    aaTCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGcc                       >  4:50773/1‑149 (MQ=255)                                                                                                                    aaTCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGcc                       <  7:346312/149‑1 (MQ=255)                                                                                                                     aTCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGccc                      <  5:326245/149‑1 (MQ=255)                                                                                                                      tCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCGGGCAATCTGccc                      >  5:109296/1‑148 (MQ=255)                                                                                                                          cTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAaca                 >  6:196614/1‑149 (MQ=255)                                                                                                                          cTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAaca                 <  5:196614/149‑1 (MQ=255)                                                                                                                             cTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGt                                            <  6:330837/119‑1 (MQ=255)                                                                                                                             cTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGt                                            >  5:330837/1‑119 (MQ=255)                                                                                                                                 aGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACACCCTTc           >  5:124049/1‑148 (MQ=255)                                                                                                                                 aGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACACCCTTc           >  8:333708/1‑148 (MQ=255)                                                                                                                                     gcgGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACCCCCTTCAAGaa      >  5:163360/1‑149 (MQ=255)                                                                                                                                        gACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACACCCTTCAAGAACaa   <  7:23453/149‑1 (MQ=255)                                                                                                                                        gACCGTCCGCGGGCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACACCCTTCAAGAACaa   <  8:166882/149‑1 (MQ=255)                                                                                                                                         aCCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGTAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACACCCTTCAAGAACAAg  >  2:341679/1‑149 (MQ=255)                                                                                                                                             |                                                                                                                                                    ATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACACCCTTCAAGAACAAG  >  NC_002947/4896282‑4896566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑