| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,896,434 | C→T | 8.2% | intergenic (+67/‑141) | PP_4304 → / → sbp‑I | VIC family cation transporter/sulfate ABC transporter substrate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,896,434 | 0 | C | T | 8.2% | 136.2 / 3.4 | 49 | intergenic (+67/‑141) | PP_4304/sbp‑I | VIC family cation transporter/sulfate ABC transporter substrate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base C (23/22); new base T (2/2); total (25/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01 | |||||||||||
CTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACACCCTTCAAGAACAAGGACGCAACGTGAA > NC_002947/4896292‑4896579 | cTGCCACAAGAAGACACACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGa < 8:106777/149‑1 (MQ=255) ccACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAg < 5:60809/147‑1 (MQ=255) ccACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGc < 1:278228/148‑1 (MQ=255) ccACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGc < 5:155501/148‑1 (MQ=255) cacaAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCgg > 1:69714/1‑149 (MQ=255) cacaAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCATCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCgg < 2:130921/149‑1 (MQ=255) agcagaCCCACGAGCAGGGGGGGGCGTTCTGTTCTCGCTGGGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGttt < 4:261988/146‑1 (MQ=255) agaagaCCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGttt < 2:69714/149‑1 (MQ=255) gaagaCCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGTGGGTAAACCCGCTCCTACGGG‑‑‑GGACCGCGCACGGTCCGCGCCTGTAGGAGCGGATTTa < 5:323825/149‑1 (MQ=17) gaagaCCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTa > 6:171315/1‑149 (MQ=255) aagaCCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTAc > 7:105112/1‑149 (MQ=255) gaCCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTAccc < 7:137442/149‑1 (MQ=255) ccACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGttt < 8:291850/142‑1 (MQ=255) ccACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGttt > 7:291850/1‑142 (MQ=255) aCGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGTGCGGGGTTTCCcgcg > 3:246457/1‑147 (MQ=255) gcggcgTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCCTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGca > 4:290029/1‑148 (MQ=255) ttctCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGGGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGaa < 8:105112/146‑1 (MQ=255) ttctCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCAAATAGCCGGTAAATAAGCAACAACAGGCGCGGACCCTGGCCGGTCC‑‑‑CCCCTAGGAGCGGGTTTTACCCCGAAGACGCCCGCACAGGCACAAGc > 4:312300/1‑148 (MQ=255) gCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGGACAGGCAACAGCATAAc > 4:213505/1‑148 (MQ=255) tgGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGG‑TTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCaaa < 2:60713/147‑1 (MQ=255) gAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCaaa > 6:272418/1‑145 (MQ=255) gAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCaaa < 5:272418/145‑1 (MQ=255) aaTGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCATAGCGGGTAAATACGCACCGACAGGCGCGGCCCGGGGGCGGTCC‑‑‑CCCGTAGGCGCGGGGTTACCCGCGAAGAGGCCGGCCCACGCAGAAGCCTaaccaaaccaat > 6:326245/1‑147 (MQ=255) gttTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGtt < 3:290029/148‑1 (MQ=255) gttTCCAAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGTTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGGGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGtt < 3:312300/148‑1 (MQ=255) ccAACGCGTGATCGCGCCTGCGCCGGCCTCTTCGCTGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTc < 7:142157/144‑1 (MQ=255) cAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATACGCTCCTACAGGCGCGGCCCGTGCGCGGTCC‑‑‑CCCGGAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCAGAACCCAAGCATTTTTTGTTCTGTAAc > 8:346312/1‑149 (MQ=38) aTAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGGTTACCCGCGAAGAGGCCGGCCCAGGCAGAAGCAGAACCAAAGCATTTTTTGTTGTTTAACCCGTATtaa > 1:109140/1‑149 (MQ=38) tctTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGc > 2:165898/1‑149 (MQ=255) ctTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCCCCCGTTATAGTCGCt > 7:162621/1‑149 (MQ=255) ttCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTg > 3:88052/1‑149 (MQ=255) tCGCGGGTAAACCCGCTCCTACGGG‑‑‑GGACCGCGCACGGTCCGCGCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTgg > 5:210295/1‑149 (MQ=17) gcgGGTAAACCCGCTCCTACGGG‑‑‑GGACCGCGCACGGTCCGCGCCTGTAGGAGCGGATTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTgg < 8:330051/147‑1 (MQ=17) gcgGGTAAACCCGCTCCTACGGG‑‑‑GGACCGCGCACGGTCCGCGCCTGTAGGAGCGGATTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTgg > 7:330051/1‑147 (MQ=17) aaTCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGcc > 4:50773/1‑149 (MQ=255) aaTCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGcc < 7:346312/149‑1 (MQ=255) aTCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGccc < 5:326245/149‑1 (MQ=255) tCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCGGGCAATCTGccc > 5:109296/1‑148 (MQ=255) cTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAaca < 5:196614/149‑1 (MQ=255) cTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAaca > 6:196614/1‑149 (MQ=255) cTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGt > 5:330837/1‑119 (MQ=255) cTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGt < 6:330837/119‑1 (MQ=255) aGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACACCCTTc > 5:124049/1‑148 (MQ=255) aGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACACCCTTc > 8:333708/1‑148 (MQ=255) gcgGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACCCCCTTCAAGaa > 5:163360/1‑149 (MQ=255) gACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACACCCTTCAAGAACaa < 7:23453/149‑1 (MQ=255) gACCGTCCGCGGGCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACACCCTTCAAGAACaa < 8:166882/149‑1 (MQ=255) aCCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGTAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACACCCTTCAAGAACAAg > 2:341679/1‑149 (MQ=255) cgGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACACCCTTCAAGAACAAGGACGCAAc < 8:162621/149‑1 (MQ=255) cccGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACACCCTTCAAGAACAAGGACGCAACGTGaa > 2:96466/1‑148 (MQ=255) | CTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACACCCTTCAAGAACAAGGACGCAACGTGAA > NC_002947/4896292‑4896579 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |