| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,936,919 | A→T | 14.2% | intergenic (‑330/‑88) | flhA ← / → PP_4345 | flagellar export pore protein/GntR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,936,919 | 0 | A | T | 14.2% | 102.0 / 7.6 | 42 | intergenic (‑330/‑88) | flhA/PP_4345 | flagellar export pore protein/GntR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (17/19); new base T (2/4); total (19/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01 | |||||||||||
TCTACGGCAATGGCCAAAGGCTTAAGCTCTTGGAAAGCCACAGAGAGGGTGGCGCCACGACAAAAAAAATCTGAGCGACTTGATCATGCAAAAGCTCACGGTTTCTATGCGGATGTAGAGAGCCAATGACTACTCAAAGGCATGAACATCATGCCAACTGCTCGATGGTAGCTCTAGTGAGTCTGTGATACCTTCGCCAGCGCTTTTGACTACATCGCTAGACCCGGAGAACATGGTGGCGAACTCTCTCGATAGCTTTATCGTTCCTGACAAACATCGCCATAAAACCGCGA > NC_002947/4936772‑4937064 | tctACGGCAATGGCCAAAGGCTTAAGCTCTTGGAAAGCCACAGAGAGGGTGGCGCCACGACAAAAAAAATCTGAGCGACTTGATCATGCAAAAGCTCACGGTTTCTATGCGGATGTAGAGAGCCAATGACTACTCAAAGGCATGAAca > 3:149418/1‑148 (MQ=255) cGGCAATGGCCAAAGGCTTAAGCTCTTGGAAAGCCACAGAGAGGGTGGCGCCACGACAAAAAAAATCTGAGCGACTTGATCATGCAAAAGCTCACGGTTTCTATGCGGATGTAGAGAGCCAATGACTACTCAAAGGCATGAAcatc < 4:343685/146‑1 (MQ=255) ggCAATGGCCAAAGGCTTAAGCTCTTTGAAAGCCACAGAGAGGGTGGCGCCACGACAAAAAAAATCTGAGCGACTTGATCATGCAAAAGCTCACGGTTTCTATGCGGATGTAGAGAGCCAATGACTACTCAAAGGCATGAACATCATGc > 8:136532/1‑149 (MQ=255) aTGGCCAAAGGCTTAAGCTCTTGGAAAGCCACAGAGAGGGTGGCGCCACGACAAAAAAAATCTGAGCGACTTGATCATGCAAAAGCTCACGGTTTCTATGCGGATGTAGAGAGCCAATGACTACTCAAAGGCATGATGTTCATGccttt < 8:278293/149‑4 (MQ=255) aTGGCCAAAGGCTTAAGCTCTTGGAAAGCCACAGAGAGGGTGGCGCCACGACAAAAAAAATCTGAGCGACTTGATCATGCAAAAGCTCACGGTTTCTATGCGGATGTAGAGAGCCAATGACTACTCAAAGGCATGATGTTCATGccttt < 1:69409/149‑4 (MQ=255) aTGGCCAAAGGCTTAAGCTCTTGGAAAGCCACAGAGAGGGTGGCGCCACGACAAAAAAAATCTGAGCGACTTGATCATGCAAAAGCTCACGGTTTCTATGCGGATGTAGAGAGCCAATGACTACTCAAAGGCATGATGTTCATGccttt < 5:99754/149‑4 (MQ=255) gCTCTTGGAAAGCCACAGAGAGGGTGGCGCCACGACAAAAAAAATCTGAGCGACTTGATCATGCAAAAGCTCACGGTTTCTATGCGGATGTAGAGAGCCAATGACTACTCAAAGGCATGAACATCATGCTAACTGCTCGATGGTAGctc < 6:89343/149‑1 (MQ=255) tctTGGAAAGCCACAGAGAGGGTGGCGCCACGACAAAAAAAATCTGAGCGACTTGATCATGCAAAAGCTCACGGTTTCTATGCGGATGTAGAGAGCCAATGACTACTCAAAGGCATGAAcatca > 6:117281/1‑124 (MQ=255) tctTGGAAAGCCACAGAGAGGGTGGCGCCACGACAAAAAAAATCTGAGCGACTTGATCATGCAAAAGCTCACGGTTTCTATGCGGATGTAGAGAGCCAATGACTACTCAAAGGCATGAAcatca < 5:117281/124‑1 (MQ=255) ttGGAAAGCAACAGAGAGGTTGGCGCAACGACAAAAAAAATCTGAGCTACTTGATCATGCAAAAGCTCACGGTTTCTATGCGTATGTAGAGAGCCAATTACTACTCAAAGGCATGAACATCATGCCAACTGCTCGATGGTAGCTCTAg < 1:73858/148‑1 (MQ=255) ggAAAGCCACAGAGATGGTGGCGCCACGACAAAAAAAATCTGAGCGACTTTATCATGCAAAAGCTCACGGTTTCTATGCGGATGTAGAGAGCCAATGACTACTCAAAGGCATGAACATCATGCCAACTGCTCGATGGTAGCTCTAGt < 7:243833/147‑1 (MQ=255) ggAAAGCCACAGAGAGGGTGGCGCCACGTCAAAAAAAATCTGAGCGACTTGATCATGCAAAAGCTCACGGTTTCTATGCGGATGTAGAGAGCCAATGACTACTCAAAGGCATGAACATCATGCCAACTGCTCGATGGTAGCTCTAGt < 5:293307/147‑1 (MQ=255) aaGCCACAGAGAGGGTGGCGCCACGACAAAAAAAATCTGAGCGACTTGATCATGCAAAAGCTCACGGTTTCTATGCGGATGTAGAGAGCCAATGACTACTCAAAGGCATGAACATCATGCCAACTGCTCGATGGTAGCTCTAGTGAGTc > 1:194757/1‑149 (MQ=255) agagGGTGGCGCCACGACAAAAAAAATCTGAGCGACTTGATCATGCAAAAGCTCACGGTTTCTATGCGGATGTAGAGAGCCAATGACTACTCAAAGGCATGAACATCATGCCAACTGCTCGATGGTAGCTCTAGTGAGTCTGTGATAcc > 4:109533/1‑149 (MQ=255) ggTGGCGCCACGACAAAAAAAATCTGAGCGACTTGATCATGCAAAAGCTCACGGTTTCTATGCGGATGTAGAGAGCCAATGACTACTCAAAGGCATGAACATCATGCCAACTGCTCGATGGTAGCTCTAGTGAGTCTGTGATACCTTCg > 8:121465/1‑149 (MQ=255) cACGACAAAAAAAATCTGAGCGACTTGATCATGCAAAAGCTCACGGTTTCTATGCGGATGTAGAGAGCCAATGACTACTCAAAGGCATGAACATCATGCCAACTGCTCGATGGTAGCTCTAGTGAGTCTGTGATACCTTCGCCAGCGCt > 2:322129/1‑149 (MQ=255) gACAAAAAAAATCTGAGCGACTTGATCATGCAAAAGCTCACGGTTTCTATGCGGATGTAGAGATCCAATGACTACTCAAAGGCATGAACATCATGCCAACTGCTCGATGGTAGCTCTAGTGAGTCTGTTATACCTTCGCCAGCGCtttt < 2:74631/149‑1 (MQ=255) cAAAAAAAATCTGAGCGACTTGATCATGCAAAAGCTCACGGTTTCTATGCGGATGTAGAGAGCCAATGACTACTCAAAGGCATGAACATCATGCCAACTGCTCGATGGTAGCTCTAGTGAGTCTGTGATACCTTCGCCAGCGCTTTTGa < 4:149418/149‑1 (MQ=255) aaaaaaaaTCTGAGCGACTTGATCATGCAAAAGCTCACGGTTTCTATGCGGATGTAGAGAGCCAATGACTACTCAAAGGCATGAACATCATGCCAACTGCTCGATGGTAGCTCTAGTGAGTCTGTGATACCTTCGCCAGCGCTTTTGAc < 5:132430/149‑1 (MQ=255) aaaaaaaaTCTGAGCGACTTGATCATGCAAAAGCTCACGGTTTCTATGCGGATGTAGAGAGCCAATGACTACTCAAAGGCATGAACATCATGCCAACTGCTCGATGGTAGCTCTAGTGAGTCTGTGATACCTTCGCCAGCGCTTTTGAc < 1:322129/149‑1 (MQ=255) gATCATGCAAAAGCTCACGGTTTCTATGCGGATGTAGAGAGCCAATGACTACTCAAAGGCATGAACATCATGCCAACTGCTCGATGGTAGCTCTAGTGAGTCTGTGATACCTTCGCCAGCGCTTTTGACTACATCGCTAGACCCGgag < 7:136532/148‑1 (MQ=255) tGCAAAAGCTCACGGTTTCTATGCGGATGTAGAGAGCCAATGACTACTCAAAGGCATGAACATCATGCCAACTGCTCGATGGTAGCTCTAGTGAGTCTGTGATACCTTCGCCAGCGCTTTTGACTACATCGCTAGACCCGGAGAAc < 6:44818/146‑1 (MQ=255) tGCAAAAGCTCACGGTTTCTATGCGGATGTAGAGAGCCAATGACTACTCAAAGGCATGAACATCATGCCAACTGCTCGATGGTAGCTCTAGTGAGTCTGTGATACCTTCGCCAGCGCTTTTGACTACATCGCTAGACCCGGAGAAc > 5:44818/1‑146 (MQ=255) tGCAAAAGCTCACGGTTTCTATGCGGATGTAGAGAGCCAATGACTACTCAAAGGCATGAACATCATGCCAACTGCTCGATGGTAGCTCTAGTGAGTCTGTGATACCTTCGCCAGCGCTTTTGACTACATCGCTAGACCCGGAGAACAtg < 3:109533/149‑1 (MQ=255) aaGCTCACGGTTTCTATGCGGATGTAGAGAGCCAATGACTACTCAAAGGCATGAACATCATGCCAACTGCTCGATGGTAGCTCTAGTGAGTCTGTGATACCTTCGCCAGCGCTTTTGACTACATCGCTAGACCCGGAGAACATGGTGGc < 2:194757/149‑1 (MQ=255) aGCTCACGGTTTCTATGCGGATGTAGAGAGCCAATGACTACTCAAAGGCATGAACATCATGCCAACTGCTCGATGGTAGCTCTAGTGAGTCTGTGATACCTTCGCCAGCGCTTTTGACTACATCGCTAGACCCGGAGAACATGGTGGCg < 5:111115/149‑1 (MQ=255) tCACGGTTTCTATGCGGATGTAGAGAGCCAATGACTACTCAAAGGCATGAACATCATCCCAACTGCTCGATGGTAGCTCTAGTGAGTCTGTGATACCTTCGCCAGCGATATTGCCTACATCGCTAGACCCGGAGAACATGGTGGCGAAc > 8:120621/1‑149 (MQ=255) gTTTCTATGCGGATGTAGAGAGCCAATGACTACTCAAAGGCATGAACATCATGCCAACTGCTCGATGGTAGCTCTAGTGAGTCTGTGATACCTTCGCCAGCGCTTTTGACTACATCGCTAGACCCGGAGAACATGGTGGCGAActctct < 7:121465/149‑1 (MQ=255) gTTTCTATGCGGATGTAGAGAGCCAATGACTACTCAAAGGCATGAACATCATGCCAACTGCTCGATGGTAGCTCTAGTGAGTCTGTGATACCTTCGCCAGCGCTTTTGACTACATCGCTAGACCCGGAGAACATGGTGGCGAActctct < 7:21395/149‑1 (MQ=255) tATGCGGATGTAGAGAGCCAATGACTACTCAAAGGCATGAACATCATGCCAACTGCTCGATGGTAGCTCTAGTGAGTCTGTGATACCTTCGCCAGCGCTTTTGACTACATCGCTAGACCCGGAGAACATGGTGGCGAACTCTCTCGa > 3:101820/1‑147 (MQ=255) ccAATGACTACTCAAAGGCATGAACATCATGCCAACTGCTCGATGGTAGCTCTAGTGAGTCTGTGATACCTTCGCCAGCGCTTTTGACTACATCGCTAGACCCGGAGAACATGGTGGCGAACTCTCTCGATAGCTTTATCGTTCCTGAc < 4:101820/149‑1 (MQ=255) cAATGACTACTCAAAGGCATGAACATCATGCCAACTGCTCGATGGTAGCTCTAGTGAGTCTGTGATACCTTCGCCAGCGCTTTTGACTa > 5:109350/1‑89 (MQ=255) cAATGACTACTCAAAGGCATGAACATCATGCCAACTGCTCGATGGTAGCTCTAGTGAGTCTGTGATACCTTCGCCAGCGCTTTTGACTa < 6:109350/89‑1 (MQ=255) ctCAAAGGCATGAACATCATGCCAACTGCTCGATGGTAGCTCTAGTGAGTCTGTGATACCTTCGCCAGCGCTTTTGACTACATCGCTAGACCCGGAGAACATGGTGGCGAACTCTCTCGATAGCTTTATCGTTCCTGACAAACATCGc > 5:18228/1‑148 (MQ=255) tCAAAGGCATGAACATCATGCCAACTGCTCGATGGTAGCTCTAGTGAGTCTGTGATACCTTCGCCAGCGCTTTTGACTACATCGCTAGACCCGGAGAACATGGTGGCGAACTCTCTCGATAGCTTTATCGTTCCTGACAAACATCGCCa > 2:144876/1‑149 (MQ=255) tCAAAGGCATGAACATCATGCCAACTACTCGATGGTAGCTCTAGTGAGTCTGTGATACCTTCGCCAGCGCTTTTGACTACATCGCTAGACCCGGAGAAc > 4:212370/1‑99 (MQ=255) tCAAAGGCATGAACATCATGCCAACTACTCGATGGTAGCTCTAGTGAGTCTGTGATACCTTCGCCAGCGCTTTTGACTACATCGCTAGACCCGGAGAAc < 3:212370/99‑1 (MQ=255) aaaGGCATGAACATCATGCCAACTGCTCGATGGTAGCTCTAGTGAGTCTGTGATACCTTCGCCAGCGCTTTTGACTACATCGCTAGACCCGGAGAACATGGTGGCGAACTCTCTCGATAGCTTTATCGTTCCTGACAAACATCGcc < 5:168641/146‑1 (MQ=255) aaaGGCATGAACATCATGCCAACTGCTCGATGGTAGCTCTAGTGAGTCTGTGATACCTTCGCCAGCGCTTTTGACTACATCGCTAGACCCGGAGAACATGGTGGCGAACTCTCTCGATAGCTTTATCGTTCCTGACAAACATCGcc > 6:168641/1‑146 (MQ=255) aaaGGCATGAACATCATGCCAACTGCTCGATGGTAGCTCTAGTGAGTCTGTGATACCTTCGCCAGCGCTTTTGACTACATCGCTAGACCCGGAGAACATGGTGGCGAACTCTCTCGATAGCTTTATCGTTCCTGACAAACATCGCCAt > 5:88744/1‑148 (MQ=255) ttggCATGATGTTCATGCCAACTGCTCGATGGTAGCTCTAGTGAGTCTGTGATACCTTCGCCAGCGCTTTTGACTACATCGCTAGACCCGGAGAACATGGTGGCGAACtctctc > 1:129292/3‑114 (MQ=255) ttggCATGATGTTCATGCCAACTGCTCGATGGTAGCTCTAGTGAGTCTGTGATACCTTCGCCAGCGCTTTTGACTACATCGCTAGACCCGGAGAACATGGTGGCGAACtctctc < 2:129292/112‑1 (MQ=255) ttggCATGATGTTCATGCCAACTGCTCGATGGTAGCTCTAGTGAGTCTGTGATACCTTCGCCAGCGCTTTTGACTACATCGCTAGACCCGGAGAACATGGTGGCGAACTCTCTCGATAGCTTTATCGTTCCTGACAAACATCGCCATa > 3:314252/3‑148 (MQ=255) aaGGCATGAACATCATGCCAACTGCTCGATGGTAGCTCTAGTGAGTCTGTGATACCTTCGCCAGCGCTTTTGACTACATCGCTAGACCCGGAGAACATGGTGGCGAACTCTCTCGATAGCTTTATCGTTCCTGACAAACATCGCCATa > 5:282655/1‑148 (MQ=255) aTGAACATCATGCCAACTGCTCGATGGTAGCTCTAGTGAGTCTGTGATACCTTCGCCAGCGCTTTTGACTACATCGCTAGACCCGGAGAACATGGTGGCGAACTCTCTCGATAGCTTTATCGTTCCTGACAAACGTCGCCATAAAACcg > 3:46361/1‑149 (MQ=255) aaCATCATGCCAACTGCTCGATGGTAGCTCTAGTGAGTCTGTGATACCTTCGCCAGCGCTTTTGACTACATCGCTAGACCCGGAGAACATGGTGGCGAACTCTCTCGATAGCTTTATCGTTCCTGACAAACATCGCCATAAAACCGCGa > 5:100661/1‑149 (MQ=255) catcatGCCAACTGCTCGATGGTAGCTCTAGTGAGTCTGTGATACCTTCGCCAGCGCTTTTGACTACATCGCTAGACCCGGAGAACATGGTGGCGAACTCTCTCGATAGCTTTATCGTTCCTGACaaa > 2:2633/1‑128 (MQ=255) catcatGCCAACTGCTCGATGGTAGCTCTAGTGAGTCTGTGATACCTTCGCCAGCGCTTTTGACTACATCGCTAGACCCGGAGAACATGGTGGCGAACTCTCTCGATAGCTTTATCGTTCCTGACaaa < 1:2633/128‑1 (MQ=255) | TCTACGGCAATGGCCAAAGGCTTAAGCTCTTGGAAAGCCACAGAGAGGGTGGCGCCACGACAAAAAAAATCTGAGCGACTTGATCATGCAAAAGCTCACGGTTTCTATGCGGATGTAGAGAGCCAATGACTACTCAAAGGCATGAACATCATGCCAACTGCTCGATGGTAGCTCTAGTGAGTCTGTGATACCTTCGCCAGCGCTTTTGACTACATCGCTAGACCCGGAGAACATGGTGGCGAACTCTCTCGATAGCTTTATCGTTCCTGACAAACATCGCCATAAAACCGCGA > NC_002947/4936772‑4937064 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |