Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 384,787 A→T 6.1% N422I (AAT→ATT)  PP_0320 → methyl‑accepting chemotaxis transducer

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947384,7870AT 6.1% 247.7 / 4.4 82N422I (AAT→ATT) PP_0320methyl‑accepting chemotaxis transducer
Reads supporting (aligned to +/- strand):  ref base A (40/37);  new base T (3/2);  total (43/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.65e-01

CCTCCGAGCGGCTGCGACGGGCCGTGGAGCAGGTAACCCAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCGGTCAGCCAGCTGG  >  NC_002947/384640‑384927
                                                                                                                                                   |                                                                                                                                            
ccTCCGAGCGGCTGCGACGGGCCGTGGAGCAGGTAACCCAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAAt                                                                                                                                             <  4:22871/149‑1 (MQ=255)
  tCCGAGCGGCTGCGACGGGCCGTGGAGCAGGTAACCCAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGc                                                                                                                                           >  3:203827/1‑149 (MQ=255)
   ccGAGCGGCTGCGACGGGCCGTGGAGCAGGTAACCCAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGcc                                                                                                                                          <  5:46225/149‑1 (MQ=255)
         ggCTGCGACGGGCCGTGGAGCAGGTAACCCAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGAt                                                                                                                                    <  4:203827/149‑1 (MQ=255)
          gCTGCGACGGGCCGTGGAGCAGGTAACCCAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATg                                                                                                                                   <  4:335735/149‑1 (MQ=255)
              cGACGGGCCGTGGAGCAGGTAACCCAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCGGCATTGCGGGCGATgtc                                                                                                                                 <  1:89758/147‑3 (MQ=255)
              cGACGGGCCGTGGAGCAGGTAACCCAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCgg                                                                                                                                <  6:16509/148‑1 (MQ=255)
                  gggCCGTGGAGCAGGTAACCCAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAg                                                                                                                           >  5:143157/1‑149 (MQ=255)
                       gTGGAGCAGGTAACCCAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTc                                                                                                                      <  5:105214/149‑1 (MQ=255)
                                     ccAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCacgag                                                                                                        >  7:114991/1‑149 (MQ=255)
                                       aGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCa                                                                                                                   <  1:53352/136‑1 (MQ=255)
                                       aGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCa                                                                                                                   >  2:53352/1‑136 (MQ=255)
                                             tggACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCg                                                                                                >  2:321665/1‑149 (MQ=255)
                                              ggACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGt                                                                                               >  8:133284/1‑149 (MQ=255)
                                                acaacaCCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTg                                                                                             <  2:269202/149‑1 (MQ=255)
                                                 caacaCCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGtt                                                                                              <  6:98778/147‑1 (MQ=255)
                                                 caacaCCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGtt                                                                                              >  5:98778/1‑147 (MQ=255)
                                                 caacaCCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTg                                                                                             >  4:185601/1‑148 (MQ=255)
                                                 caacaCCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGc                                                                                            <  8:114991/149‑1 (MQ=255)
                                                    cacCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAgg                                                                                         >  5:108228/1‑149 (MQ=255)
                                                     acCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGc                                                                                        <  2:41413/149‑1 (MQ=255)
                                                     acCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGc                                                                                        >  8:37251/1‑149 (MQ=255)
                                                              cgcTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAggt                                                                               <  6:354111/149‑1 (MQ=255)
                                                               gcTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAggtg                                                                              >  2:304951/1‑149 (MQ=255)
                                                               gcTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAggtg                                                                              <  7:37251/149‑1 (MQ=255)
                                                                cTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAgg                                                                                <  1:134262/146‑1 (MQ=255)
                                                                cTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAgg                                                                                >  2:134262/1‑146 (MQ=255)
                                                                 tCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCG‑TGCAGGCCCGCGAGgtggtg                                                                           >  2:319048/1‑149 (MQ=255)
                                                                   cGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAggtggt                                                                            <  3:326941/147‑1 (MQ=255)
                                                                   cGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGgtggtg                                                                           >  3:13502/1‑148 (MQ=255)
                                                                   cGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGc                                                                          <  5:209734/149‑1 (MQ=255)
                                                                         ccAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGAt                                                                    <  1:321665/149‑1 (MQ=255)
                                                                            gcagGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAg                                                                                          <  3:157462/124‑1 (MQ=255)
                                                                            gcagGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAg                                                                                          >  4:157462/1‑124 (MQ=255)
                                                                            gcagGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCa                                                                 >  5:72406/1‑149 (MQ=255)
                                                                              agGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCTCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCAtt                                                               >  8:227498/1‑149 (MQ=255)
                                                                               ggAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTc                                                              <  1:77186/149‑1 (MQ=255)
                                                                                gagaTGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCg                                                             >  2:157046/1‑149 (MQ=255)
                                                                                 agaTGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGt                                                            <  6:72406/149‑1 (MQ=255)
                                                                                  gaTGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTg                                                           <  4:13502/149‑1 (MQ=255)
                                                                                    tGACCGAAATGGTCGCCACTGCCGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTgg                                                          >  5:269690/1‑148 (MQ=255)
                                                                                       ccGAAATGGTCGCCACTGCCGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCAt                                                       <  6:269690/148‑1 (MQ=255)
                                                                                             tGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGc                                                <  6:108228/149‑1 (MQ=255)
                                                                                               gTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCAt                                              >  8:155435/1‑149 (MQ=255)
                                                                                                tCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATg                                             >  8:170690/1‑149 (MQ=255)
                                                                                                  gCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTc                                                              <  4:25642/130‑1 (MQ=255)
                                                                                                  gCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTc                                                              >  3:25642/1‑130 (MQ=255)
                                                                                                     aCTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGt                                                            <  7:325499/129‑1 (MQ=255)
                                                                                                     aCTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGt                                                            >  8:325499/1‑129 (MQ=255)
                                                                                                         ctgTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAggtg                                                                              <  7:251922/107‑1 (MQ=255)
                                                                                                         ctgTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAggtg                                                                              >  8:251922/1‑107 (MQ=255)
                                                                                                          tgTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACa                                   >  6:29457/1‑149 (MQ=255)
                                                                                                              cacGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCgg                               >  1:155715/1‑149 (MQ=255)
                                                                                                              cacGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAAGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGACATCGAGGGCATGTCGGGCGACATCgg                               >  4:338688/1‑149 (MQ=255)
                                                                                                               acGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCgg                               >  1:354859/1‑148 (MQ=255)
                                                                                                               acGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCgg                               >  2:101697/1‑148 (MQ=255)
                                                                                                               acGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATCCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGACGTTGCAGGCCCGCCAGGTGGAGCAACGAACCATTCGTCGCAACGAGGGCATGTCGGGCGACATCgg                               >  2:242315/1‑148 (MQ=255)
                                                                                                                 gagaTGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCaa                            <  5:29457/149‑1 (MQ=255)
                                                                                                                 gagaTGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCaa                            <  3:185601/149‑1 (MQ=255)
                                                                                                                   gaTGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAgg                          <  1:101697/149‑1 (MQ=255)
                                                                                                                       ggCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCCCGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATc                                                      >  8:342371/1‑117 (MQ=255)
                                                                                                                       ggCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATc                                                      <  7:342371/117‑1 (MQ=255)
                                                                                                                          cTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAg                                                                                 <  3:18432/87‑1 (MQ=255)
                                                                                                                          cTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAg                                                                                 >  4:18432/1‑87 (MQ=255)
                                                                                                                          cTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAgg                          <  3:234340/142‑1 (MQ=255)
                                                                                                                          cTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAgg                          >  4:234340/1‑142 (MQ=255)
                                                                                                                            gACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAggcg                        >  3:163915/1‑142 (MQ=255)
                                                                                                                            gACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAggcg                        <  4:163915/142‑1 (MQ=255)
                                                                                                                             aCCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCG‑TGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATg                 <  1:319048/147‑1 (MQ=255)
                                                                                                                               cGTGCAGGAGATCGCCCGCAATGCCGGCGATGCGGCCCTGGCGTCGCAGTCGGCACGAGTCGAGGGGTTGCTGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCgg              <  7:133284/149‑1 (MQ=255)
                                                                                                                               cGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCgg              <  1:304951/149‑1 (MQ=255)
                                                                                                                                  gCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCGGt             >  8:76066/1‑147 (MQ=255)
                                                                                                                                  gCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCGGt             >  7:71118/1‑147 (MQ=255)
                                                                                                                                   cAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCGGTcag          >  6:99416/1‑149 (MQ=255)
                                                                                                                                     ccgcATCGCCGGCATTGCGGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCGGTcagc         >  4:230091/4‑148 (MQ=255)
                                                                                                                                      gACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGTAAGGCGGCCGATGCGGTcagc         >  1:99886/1‑147 (MQ=255)
                                                                                                                                        cATCGCCGGCATTGCGGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGc                                       >  1:279457/1‑115 (MQ=255)
                                                                                                                                        cATCGCCGGCATTGCGGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGc                                       <  2:279457/115‑1 (MQ=255)
                                                                                                                                        cATCGCCGGCATTGCGGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGAGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCGGTcagccagc     >  8:184173/1‑149 (MQ=255)
                                                                                                                                         aTCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCGGTCAGCCAGCt    >  7:28854/1‑149 (MQ=255)
                                                                                                                                         aTCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGATGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCGGTCAGCCAGCt    >  5:341172/1‑149 (MQ=255)
                                                                                                                                            gCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCGGTCAGCCAGCTgg  <  7:155435/148‑1 (MQ=255)
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CCTCCGAGCGGCTGCGACGGGCCGTGGAGCAGGTAACCCAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCGGTCAGCCAGCTGG  >  NC_002947/384640‑384927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑