Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,976,230 C→T 8.1% A181T (GCC→ACC)  flgJ ← peptidoglycan hydrolase FlgJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,976,2300CT 8.1% 139.5 / 3.0 49A181T (GCC→ACC) flgJpeptidoglycan hydrolase FlgJ
Reads supporting (aligned to +/- strand):  ref base C (19/26);  new base T (2/2);  total (21/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01

ATGGTGGCGACGAAGGCGTCGCTGTCGGTGAAAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGTTGAGTGCAGCAACGTCGTTGCGCGTAGCGGACGAGGCCATGGCATGCGCTGGCTCCGC  >  NC_002947/4976082‑4976371
                                                                                                                                                    |                                                                                                                                             
aTGGTGGCGACGAAGGCGTCGCTGTCGGTGAAAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACggcggc                                                                                                                                               <  2:272609/149‑1 (MQ=255)
 tggtggCGACGAAGGCGTCGCTGTCGGTGAAAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGgcggcg                                                                                                                                              >  1:233758/1‑149 (MQ=255)
      ggtagtaaGGGGTGGGTGTCGGTGAATGCTTTTTTGGGGGCCTGCGGTGGCTGGGCCAGGGAAGGGCGTAAAATGGGCTGGCGGTTGTCCGGCAGGGCTATGGCCTTGGCTACTGCGTGACCTTCAGGGGCAGGCACGGCGGCGTTAtt                                                                                                                                         <  2:196070/143‑1 (MQ=255)
      gCGACGATGGCGTCGCTGTCGGTGATCGCCTTGTTCGGCGCGAGCGGTGGCGGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCAGGGGCAGGCACGGCGGCGTTAtt                                                                                                                                         <  2:47697/149‑1 (MQ=255)
      gCGACGACGGCGTCGCTGGCGGTCAACGCCTTGTGCGGCCCCCCCGGTGGCTGGCCCAGGCCACGGCCGACAATGCGCCGGCCGTTGCCCGGCACGGCGAAGGCCTTGCCTACTGCCTGACCGTCCCGGGCAGGCACGGCGGCGTTAtt                                                                                                                                         <  4:167665/149‑1 (MQ=255)
       cgacgaAGGCGTCGCTGTCGGTGAAAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTg                                                                                                                                        <  2:43156/149‑1 (MQ=255)
         acgaAGGCGTCGCTGTCGGTGAAAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGc                                                                                                                                      <  2:233758/149‑1 (MQ=255)
          cgaAGGCGTCGCTGTCGGTGAAAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCa                                                                                                                                     <  7:61936/149‑1 (MQ=255)
          cgaAGGCGTCGCTGTCGGTGAAAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCa                                                                                                                                     <  4:288569/149‑1 (MQ=255)
           gaAGGCGTCGCTGTCGGTGAAAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGgcggcg                                                                                                                                              >  7:242557/1‑139 (MQ=255)
           gaAGGCGTCGCTGTCGGTGAAAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGgcggcg                                                                                                                                              <  8:242557/139‑1 (MQ=255)
           gaAGGCGTCGCTGTCGGTGAAAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGTGTTATTGGCAg                                                                                                                                    >  3:250845/1‑149 (MQ=255)
           gaAGGCGTCGCTGTCGGTGAAAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGTGTTATTGGCAg                                                                                                                                    >  3:246020/1‑149 (MQ=255)
                   cgttctCGGTGAAAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTccgcc                                                                                                                            <  1:127756/144‑1 (MQ=255)
                          ggTGAAAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTccgcc                                                                                                                            >  2:127756/1‑142 (MQ=255)
                          ggTGAAAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCa                                                                                                                     >  7:344250/1‑149 (MQ=255)
                                aGCCTTGTTCGGCGCCCGCGGGGGCGGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATAc                                                                                                               <  8:344250/149‑1 (MQ=255)
                                      gTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGtt                                                                                                                                  >  5:40343/1‑124 (MQ=255)
                                         cGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCaa                                                                                                        >  7:327492/1‑147 (MQ=255)
                                           gcgcCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAgg                                                                                                    <  1:300801/149‑1 (MQ=255)
                                           gcgcCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAgg                                                                                                    <  5:271704/149‑1 (MQ=255)
                                                 gCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGcggtcg                                                                                              >  3:134098/1‑149 (MQ=255)
                                                         tGGGCCACGCCACGGCCCACAATGCGCCGGCCGTTGGCCGGCACGGCGACGGCCTTGGCTACTGCCTGACCGTCCCGGGCAGGCACGGCGGTGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGttt                                                                                      <  4:250845/149‑1 (MQ=255)
                                                         ggggCCACGGCACGGCCTAAAATGCGCCGGCGGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGCCCTTCACGGGCCGGCACGGCGGTGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGttt                                                                                      <  4:246020/148‑1 (MQ=255)
                                                             ccACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCtt                                                                                  <  8:327492/149‑1 (MQ=255)
                                                              cacggcacggCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTcc                                                                                                                               <  2:46401/103‑1 (MQ=255)
                                                              cacggcacggCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTcc                                                                                                                               >  1:46401/1‑103 (MQ=255)
                                                                             aaTGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGcgcc                                                                  >  4:216709/1‑149 (MQ=255)
                                                                              gggccccggCCGGTGTCCGGCACGGCGAAGGCCTTGGCTCCTGCCTGCCCGTCACGGGCCGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGcgccg                                                                 <  4:5652/142‑1 (MQ=255)
                                                                                  gcAGGCCGTGGTCCGGCACGGCGAGGGCCTTGGCTACTGCCTGGCCGTCGCGGGGAGGCACGGCGGCGGGATTGGCAGTTTCCGCCGACGGCACGATACGGGCCGGCAGGCGGGCGGTGGGTTTGCGTGGCAGGGCCAGGCGCcgcggg                                                             >  4:289037/1‑147 (MQ=255)
                                                                                       ccGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGTTGAg                                                        <  5:150029/149‑1 (MQ=255)
                                                                                        cGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTgg                                                                                <  5:195842/124‑1 (MQ=255)
                                                                                        cGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTgg                                                                                >  6:195842/1‑124 (MQ=255)
                                                                                            gTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGTTGAGTgca                                                    <  2:151565/148‑1 (MQ=255)
                                                                                             gccGGCAGGGCGATGGGCTTGGCTACTCCCTGACCGTCCCGGGCACGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGTTGAGTgcagc                                                  <  2:360762/148‑1 (MQ=255)
                                                                                                 gCACGGCGAAGGCCTTGGCTACTGCCTTACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGTTGAGTgc                                                     >  1:10687/1‑142 (MQ=255)
                                                                                                 gCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGTTGAGTgc                                                     <  2:10687/142‑1 (MQ=255)
                                                                                                      gCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGTTGAGTGCAGCAACGTCGtt                                         <  2:235380/149‑1 (MQ=255)
                                                                                                         aaGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGTTGAGTGCAGCAACGTCGTTgcg                                      >  1:217286/1‑149 (MQ=255)
                                                                                                          aGGCCTTGGCTACTGCCGGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGTTGAGTGCAGCAACGTCGTTgcgc                                     <  8:81835/149‑1 (MQ=255)
                                                                                                              ctaggCTACTGCCTGACCGTCACGGCCAGGCACGGCGGCGTTAATGGCAGATTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGCTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGTTGAGTgca                                                    >  1:72216/4‑130 (MQ=255)
                                                                                                                   gccgtgcctgccCGTGACGGTCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGTTGAGTGCAGCAACGTCGTTGCGCGTAGCGGAc                            >  5:95623/5‑149 (MQ=255)
                                                                                                                   cTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGTTGAGTGCAGCAACGTCGTTGCGCGTAGCGGAc                            >  6:300331/1‑149 (MQ=255)
                                                                                                                    tACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGTTGAGTGCAGCAACGTCGTTGCGCGTAGCGGAc                            >  6:345119/1‑148 (MQ=255)
                                                                                                                           tGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAg                                                                       <  8:238637/98‑1 (MQ=255)
                                                                                                                             aCCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGTTGAGTGCAGCAACGTCGTTGCGCGTAGCGGACGAGGCCATgg                  <  3:289037/149‑1 (MQ=255)
                                                                                                                                  cACGGGCAGGCACGGCGGCGTTTTTGGCAGTTTCCGCCGACGGCACGATACGGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGTTGAGTGCAGCAACGTCGTTgcgc                                     <  7:125763/125‑1 (MQ=255)
                                                                                                                                  cACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGTTGAGTGCAGCAACGTCGTTgcgc                                     >  8:125763/1‑125 (MQ=255)
                                                                                                                                           gcaCGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGTTGAGTGCAGCAACGTCGTTGCGCGTAGCGGACGAGGCCATGGCATGCGCTGGCTcc    >  6:46063/1‑149 (MQ=255)
                                                                                                                                           gcaCGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGTTGAGTGCAGCAACGTCGTTGCGCGTAGCGGACGAGGCCATGGCATGCGCTGGCTcc    >  6:69029/1‑149 (MQ=255)
                                                                                                                                             aCGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGTTGAGTgca                                                    <  1:347681/99‑1 (MQ=255)
                                                                                                                                             aCGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGTTGAGTgca                                                    >  2:347681/1‑99 (MQ=255)
                                                                                                                                             aCGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGTTGAGTGCAGCAACGTCGTTGCGCGTAGCGGACGAGGCCATGGCATGCGCTGGCTCCGc  <  5:69029/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
ATGGTGGCGACGAAGGCGTCGCTGTCGGTGAAAGCCTTGTTCGGCGCCAGCGGTGGCTGGGCCACGGCACGGCCGACAATGCGCAGGCCGTTGTCCGGCACGGCGAAGGCCTTGGCTACTGCCTGACCGTCACGGGCAGGCACGGCGGCGTTATTGGCAGTTTCCGCCGACGGCACGATACCGGCCAACAGGCGGTCGGTGAGTTTGCTTGGCAGGGCCAGGCGCCGCGCGTTGAGTGCAGCAACGTCGTTGCGCGTAGCGGACGAGGCCATGGCATGCGCTGGCTCCGC  >  NC_002947/4976082‑4976371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑