Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 402,756 A→G 7.8% Y148H (TAC→CAC)  msrA ← peptide methionine sulfoxide reductase MsrA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947402,7560AG 7.8% 177.2 / 4.7 64Y148H (TAC→CAC) msrApeptide methionine sulfoxide reductase MsrA
Reads supporting (aligned to +/- strand):  ref base A (29/30);  new base G (2/3);  total (31/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.44e-01

GCTGGTGGTAGGCCTCGGCGAAATAGACGGTCGGTGCCTGGTCGATTTCGGTGGTGATCTCACCGAAGCCGGCCTTGCTCAGCTCGGCCTGGAAGGCGTCGCGGCTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGGCGCATACCTTGGGTGGGGTTGTGCAGCTCCCAGAACATTGCCAGCAGTTCGCGGTAGCTGACCTTGTCCTTGTCGAACACCACCAGCACCACTTCGGTGTGGCCGGT  >  NC_002947/402612‑402903
                                                                                                                                                |                                                                                                                                                   
gctgGTGGTAGGCCTCGGCGAAATAGACGGTCGGTGCCTGGTCGATTTCGGTGGTGATCTCACCGAAGCCGGCCTTGCTCAGCTCGGCCTGGAAGGCGTCGCGGCTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTGGATg                                                                                                                                                 >  6:112193/1‑149 (MQ=255)
 ctgGTGGTAGGCCTCGGCGAAATAGACGGTCGGTGCCTGGTCGATTTCGGTGGTGATCTCACCGAAGCCGGCCTTGCTCAGCTCGGCCTGGAAGGCGTCGCGGCTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATgg                                                                                                                                                >  4:214291/1‑149 (MQ=255)
          ggCCTCGGCGAAATAGACGGTCGGTGCCTGGTCGATTTCGGTGGTGATCTCACCGAAGCCGGCCTTGCTCAGCTCGGCCTGGAAGGCGTCGCGGCTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGt                                                                                                                                       <  5:233778/149‑1 (MQ=255)
                 gCGAAATAGACGGTCGGTGCCTGGTCGATTTCGGTGGTGATCTCACCGAAGCCGGCCTTGCTCAGCTCGGCCTGGAAGGCGTCGCGGCTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGt                                                                                                                                <  3:184026/149‑1 (MQ=255)
                   gAAATAGACGGTCGGTGCCTGGTCGATTTCGGTGGTGATCTCACCGAAGCCGGCCTTGCTCAGCTCGGCCTGGAAGGCGTCGCGGCTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGc                                                                                                                              >  7:112017/1‑149 (MQ=255)
                    aaaTAGTCGGTCGTTGGCTGGTCGATTTCGGTGGTTATCTCACCGAAGCCGGCCTTGCTCAGCTCGGCCTGGAAGGCGTCGCGGCTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGcc                                                                                                                             <  8:112017/149‑1 (MQ=255)
                    aaaTAGACGGTCGGTGCCTGGTCGATTTCGGTGGTGATCTCACCGAAGCCGGCCTTGCTCAGCTCGGCCTGGAAGGCGTCGCGGCTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTgg                                                                                                                                  >  1:317507/1‑144 (MQ=255)
                    aaaTAGACGGTCGGTGCCTGGTCGATTTCGGTGGTGATCTCACCGAAGCCGGCCTTGCTCAGCTCGGCCTGGAAGGCGTCGCGGCTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTgg                                                                                                                                  <  2:317507/144‑1 (MQ=255)
                        aGACGGTCGGTGCCTGGTCGATTTCGGTGGTGATCTCACCGAAGCCGGCCTTGCTCAGCTCGGCCTGGAAGGCGTCGCGGCTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATg                                                                                                                         >  8:42934/1‑149 (MQ=255)
                        aGACGGTCGGTGCCTGGTCGATTTCGGTGGTGATCTCACCGAAGCCGGCCTTGCTCAGCTCGGCCTGGAAGGCGTCGCGGCTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATg                                                                                                                         >  8:38864/1‑149 (MQ=255)
                            ggtcggtGCCTGGTCGATTTCGGTGGTGATCTCACCGAAGCCGGCCTTGCTCAGCTCGGCCTGGAAGGCGTCGCGGCTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTCGt                                                                                                                     >  2:266042/1‑149 (MQ=255)
                                     cTGGTCGATTTCGGTGGTGATCTCACCGAAGCCGGCCTTGCTCAGCTCGGCCTGGAAGGCGTCGCGGCTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGgc                                                                                                            >  1:80644/1‑149 (MQ=255)
                                      tGGTCGATTTCGGTGGTGATCTCACCGAAGCCGGCCTTGCTCAGCTCGGCCTGGAAGGCGTCGCGGCTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGgcg                                                                                                           >  6:147409/1‑149 (MQ=255)
                                      tGGTCGATTTCGGTGGTGATCTCACCGAAGCCGGCCTTGCTCAGCTCGGCCTGGAAGGCGTCGCGGCTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGgcg                                                                                                           >  4:69907/1‑149 (MQ=255)
                                      tGGTCGATTTCGGTGGTGATCTCACCGAAGCCGGCCTTGCTCAGCTCGGCCTGGAAGGCGTCGCGACTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGgcg                                                                                                           >  8:159669/1‑149 (MQ=255)
                                        gTCGATTTCGGTGGTGATCTCACCGAAGCCGGCCTTGCTCAGCTCGGCCTGGAAGGCGTCGCGGCTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGGCGCa                                                                                                         <  3:214291/149‑1 (MQ=255)
                                         tCGATTTCGGTGGTGATCTCACCGAAGCCGGCCTTGCTCAGCTCGGCCTGGAAGGCGTCGCGGCTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGgc                                                                                                            >  6:157149/1‑145 (MQ=255)
                                         tCGATTTCGGTGGTGATCTCACCGAAGCCGGCCTTGCTCAGCTCGGCCTGGAAGGCGTCGCGGCTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGgc                                                                                                            <  5:157149/145‑1 (MQ=255)
                                           gATTTCGGTGGTGATCTCACCGAAGCCGGCCTTGCTCAGCTCGGCCTGGAAGGCGTCGCGGCTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTCGtt                                                                                                                    <  4:332573/135‑1 (MQ=255)
                                           gATTTCGGTGGTGATCTCACCGAAGCCGGCCTTGCTCAGCTCGGCCTGGAAGGCGTCGCGGCTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTCGtt                                                                                                                    >  3:332573/1‑135 (MQ=255)
                                           gATTTCGGTGGTGATCTCACCGAAGCCGGCCTTGCTCAGCTCGGCCTGGAAGGCGTCGCGGCTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTCGtt                                                                                                                    <  2:267161/135‑1 (MQ=255)
                                           gATTTCGGTGGTGATCTCACCGAAGCCGGCCTTGCTCAGCTCGGCCTGGAAGGCGTCGCGGCTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTCGtt                                                                                                                    >  1:267161/1‑135 (MQ=255)
                                               tCGGTGGTGATCTCACCGAAGCCGGCCTTGCTCAGCTCGGCCTGGAAGGCGTCGCGGCTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGGCGCATACCtt                                                                                                   <  7:38864/148‑1 (MQ=255)
                                                 ggtggtGATCTCACCGAAGCCGGCCTTGCTCAGCTCGGCCTGGAAGGCGTCGCGGCTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGGCGCATACCTtgg                                                                                                 <  2:80644/148‑1 (MQ=255)
                                                   tggtgATCTCACCGAAGCCGGCCTTGCTCAGCTCGGCCTGGAAGGCGTCGCGGCTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGGCGCATACCTtgggtg                                                                                              <  4:258581/149‑1 (MQ=255)
                                                      tgATCTCACCGAAGCCGGCCTTGCTCAGCTCGGCCTGGAAGGCGTCGCGGCTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGGCGCATACCTTGGGTgggg                                                                                           <  2:166784/149‑1 (MQ=255)
                                                          ctcACCGAAGCCGGCCTTGCTCAGCTCGGCCTGGAAGGCGTCGCGGCTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTc                                                                                                                       <  4:15343/117‑1 (MQ=255)
                                                          ctcACCGAAGCCGGCCTTGCTCAGCTCGGCCTGGAAGGCGTCGCGGCTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTc                                                                                                                       >  3:15343/1‑117 (MQ=255)
                                                             aCCGAAGCCGGCCTTGCTCAGCTCGGCCTGGAAGGCGTCGCGGCTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGGCGCATACCTTGGGTGGGGTTGTGCa                                                                                    <  7:42934/149‑1 (MQ=255)
                                                                     cGGCCTTGCTCAGCTCGGCCTGGAAGGCGTCGCGGCTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGGCGCATACCTTGGGTGGGGTTGTGCAGCTcc                                                                               >  2:190446/1‑146 (MQ=255)
                                                                      ggCCTTGCTCAGCTCGGCCTGGAAGGCGTCGCGGCTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGcc                                                                                                                                              <  3:343863/82‑1 (MQ=255)
                                                                      ggCCTTGCTCAGCTCGGCCTGGAAGGCGTCGCGGCTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGcc                                                                                                                                              >  4:343863/1‑82 (MQ=255)
                                                                          ttGCTCAGCTCGGCCTGGAAGGCGTCGCGGCTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGGCGCATACCTTGGGTGGGGTTGTGCAGCTCCCAGAACAt                                                                       >  8:36879/1‑149 (MQ=255)
                                                                            gCTCAGCTCGGCCTGGAAGGCGTCGCGGCTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGGCGCATACCTtgggtggg                                                                                            >  5:279299/1‑126 (MQ=255)
                                                                            gCTCAGCTCGGCCTGGAAGGCGTCGCGGCTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGGCGCATACCTtgggtggg                                                                                            <  6:279299/126‑1 (MQ=255)
                                                                            gCTCAGCTCGGCCTGGAAGGCGTCGCGGCTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGGCGCATACCTtgg                                                                                                 >  4:206765/1‑121 (MQ=255)
                                                                            gCTCAGCTCGGCCTGGAAGGCGTCGCGGCTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGGCGCATACCTtgg                                                                                                 <  3:206765/121‑1 (MQ=255)
                                                                                aGCTCGGCCTGGAAGGCGTCGCGGCTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTGGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGGCGCATACCTTGGGTGGGGTTGTGCAGCTCCCAGAACATTGCcag                                                                 >  7:13151/1‑149 (MQ=255)
                                                                                   tCGGCATGTAAGGCGTCTCGGCTGGCATTGGCCTGTTCCAGATGTTCCGGGATGTTGCAGTACATGGCAGAGCGCTAATGGGTGCCTATGTCGTTGACCTGCCGCATACCTTCGCAGGGGTTATCAAGCTCCCAGAACATTGCcagcag                                                              >  4:336022/1‑149 (MQ=255)
                                                                                    cGGCCTGGAAGGCGACGCGGCTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGGCGCATACCTTGGGTGGGGTTGTGCAGCTCCCAGAACATTGCCAGCAGt                                                             >  4:148177/1‑149 (MQ=255)
                                                                                        cTGGAAGGCGTCGCGGCTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTGGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGGCGCATACCTTGGGTGGGGTTGTGCAGCTCCCAGAACATTGCCAGCAGTTcgc                                                         <  8:13151/149‑1 (MQ=255)
                                                                                        cTGGAAGGCGTCGCGACTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGGCGCATACCTTGGGTGGGGTTGTGCAGCTCCCAGAACATTGCCAGCAGTTcgc                                                         <  7:159669/149‑1 (MQ=255)
                                                                                          ggAAGGCGTCGCGGCTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGGCGCATACCTTGGGTGGGGTTGTGCAGCTCCCAGAACATTGCCAGCAGTTCGCgg                                                       >  4:97899/1‑149 (MQ=255)
                                                                                            aaGGCGTCGCGGCTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTGGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGGCGCATACCTTGGGTGGGGTTGTGCAGCTCCCAGAACATTGCCAGCAGTTCGCGGTa                                                     <  5:112193/149‑1 (MQ=255)
                                                                                              ggCGTCGCGGCTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGGCGCATACCTTGGGTGGGGTTATGCAGCTCCCAGAACATTGCCAGCAGTTCGCGGTAGc                                                   <  3:336022/149‑1 (MQ=255)
                                                                                                        cTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGGCGCATACCTTGGGTGGGGTTGTGCAGCTCCCAGAACATTGCCAGCAGTTCGCGGTAGCTGAccttgtc                                         <  3:69907/149‑1 (MQ=255)
                                                                                                           gccttggcctGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGGCGCATACCTTGGGTGGGGTTGTGCAGCTCCCAGAACATTGCCAGCAGTTCGCGGTAGCTGAccttgtcc                                        <  3:97899/147‑1 (MQ=255)
                                                                                                             cttggcctGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGGCGCATACCTTGGGTGGGGTTGTGCAGCTCCCAGAACATTGCCAGCAGTTCGCGGTAGCTGAccttgtccttgt                                    <  7:36879/149‑1 (MQ=255)
                                                                                                             cttggcctGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGGCGCATACCTTGGGTGGGGTTGTGCAGCTCCCAGAACATTGCCAGCAGTTCGCGGTAGCTGAccttgtc                                         >  8:20630/1‑144 (MQ=255)
                                                                                                             cttggcctGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGGCGCATACCTTGGGTGGGGTTGTGCAGCTCCCAGAACATTGCCAGCAGTTCGCGGTAGCTGAccttgtc                                         <  7:20630/144‑1 (MQ=255)
                                                                                                                ggcctGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGGCGCATACCTTGGGTGGGGTTGTGCAGCTCCCAGAACATTGCCAGCAGTTCGCGGTAGCTGACCTTGTCCTTGTCg                                  <  1:190446/148‑1 (MQ=255)
                                                                                                                    tGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGGCGCATACCTTGGGTGGGGTTGTGCAGCTCCCAGAACATTGCCAGCAGTTCGCGGTAGCTGACCTTGTCCTTGTCGAacac                             >  6:327733/1‑149 (MQ=255)
                                                                                                                        ccAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGGCGCATACCTTGGGTGGGGTTGTGCAGCTCCCAGAACATTGCCAGCAGTTCGCGGTAGCTGACCTTGTCCTTGTCGAAcaccac                          <  5:327733/148‑1 (MQ=255)
                                                                                                                               ttCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGGCGCATACCTTGGGTGGGGTTGTGCAGCTCCCAGAACATTGCCAGCAGTTCGCGGTAGCTGAccttgtccttgt                                    >  1:86685/1‑131 (MQ=255)
                                                                                                                               ttCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGGCGCATACCTTGGGTGGGGTTGTGCAGCTCCCAGAACATTGCCAGCAGTTCGCGGTAGCTGAccttgtccttgt                                    <  2:86685/131‑1 (MQ=255)
                                                                                                                               ttCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGGCGCATACCTTGGGTGGGGTTGTGCAGCTCCCAGAACATTGCCAGCAGTTCGCGGTAGCTGACCTTGTCCTTGTCGAACACCACCAGcacca                  >  2:74766/1‑149 (MQ=255)
                                                                                                                               ttCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGGCGCATACCTTGGGTGGGGTTGTGCAGCTCCCAGAACATTGCCAGCAGTTCGCGGTAGCTGACCTTGTCCTTGTCGAACACCACCAGcacc                   <  5:147409/148‑1 (MQ=255)
                                                                                                                                      cTGGTGCAGTGGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGGCGCATACCTTGGGTGGGGTTGTGCAGCTCCCAGAACATTGCCAGCAGTTCGCGGTAGCTGACCTTGTCCTTGTCGAACACCACCAGCACCACTTCGgt           <  2:177008/149‑1 (MQ=255)
                                                                                                                                         gTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGGCGCATACCTTGGGTGGGGTTGTGCAGCTCCCAGAACATTGCCAGCAGTTCGCGGTAGCTGACCTTGTCCTTGTCGAACACCACCAGCACCACTTCGgtgt         <  8:284978/148‑1 (MQ=255)
                                                                                                                                            cAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGGCGCATACCTTGGGTGGGGTTGTGCAGCTCCCAGAACATTGCCAGCAGTTCGCGGTAGCTGACCTTGTCCTTGTCg                                  <  2:229113/120‑1 (MQ=255)
                                                                                                                                            cAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGGCGCATACCTTGGGTGGGGTTGTGCAGCTCCCAGAACATTGCCAGCAGTTCGCGGTAGCTGACCTTGTCCTTGTCg                                  >  1:229113/1‑120 (MQ=255)
                                                                                                                                            cAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGGCGCATACCTTGGGTGGGGTTGTGCAGCTCCCAGAACATTGCCAGCAGTTCGCGGTAGCTGACCTTGTCCTTGTCGAACACCACCAGCACCACTTCGGTGTGGc      <  3:148177/148‑1 (MQ=255)
                                                                                                                                               tAGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGGCGCATACCTTGGGTGGGGTTGTGCAGCTCCCAGAACATTGCCAGCAGTTCGCGGTAGCTGACCTTGTCCTTGTCGAACACCACCAGCACCACTTCGGTGTGGCCGGt  >  7:230471/1‑149 (MQ=255)
                                                                                                                                               tAGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGGCGCATACCTTGGGTGGGGTTGTGCAGCTCCCAGAACATTGCCAGCAGTTCGCGGTAGCTGACCTTGTCCTTGTCGAACACCACCAGCACCACTTCGGTGTGGCCGGt  >  3:208765/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
GCTGGTGGTAGGCCTCGGCGAAATAGACGGTCGGTGCCTGGTCGATTTCGGTGGTGATCTCACCGAAGCCGGCCTTGCTCAGCTCGGCCTGGAAGGCGTCGCGGCTGGCCTTGGCCTGTTCCAGCTGTTCCGGGCTGGTGCAGTAGATGGCCGAGCGGTACTGGGTGCCGATGTCGTTGCCCTGGCGCATACCTTGGGTGGGGTTGTGCAGCTCCCAGAACATTGCCAGCAGTTCGCGGTAGCTGACCTTGTCCTTGTCGAACACCACCAGCACCACTTCGGTGTGGCCGGT  >  NC_002947/402612‑402903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑