Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,085,539 A→G 6.4% V40A (GTG→GCG)  astE ← succinylglutamate desuccinylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,085,5390AG 6.4% 174.7 / 2.5 62V40A (GTG→GCG) astEsuccinylglutamate desuccinylase
Reads supporting (aligned to +/- strand):  ref base A (25/33);  new base G (2/2);  total (27/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.48e-01

GCACCCGCGCCCTGGGCCTGATCTTGCCGTTGGCCACGCCGTGCAGCAGCCGCTCGAGCAATTCGATCGGCGCTGTTTCATTGCCATGAATGCC‑GGCGGACAACAGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGGTGTCACTTGGGTCTTCTCGGCCGGTTCGTGATCGGTGAGGGTCAGCTCAAGCAATTTGCCAAGGGCGAGCATAGGTGCTTCCTTAGTGGTGGT  >  NC_002947/5085396‑5085678
                                                                                                                                                |                                                                                                                                           
gCACCCGCGCCCTGGGCCTGATCTTGCCGTTGGCCACGCCGTGCAGCAGCCGCTCGAGCAATTCGATCGGCGCTGTTTCATTGCCATGAATGCC‑GGCGGACAACAGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCGCtcg                                                                                                                                         >  6:144208/1‑146 (MQ=255)
   cccGCGCCCTGGGCCTGATCTTGCCGTTGGCCACGCCGTGCAGCAGCCGCTCGAGCAATTCGATCGGCGCTGTTTCATTGCCATGAATGCC‑GGCGGACAACAGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCACTTCGAgc                                                                                                                                     <  7:146008/149‑1 (MQ=255)
      gcgcCCTGGGCCTGATCTTGCCGTTGGCCACGCCGTGCAGCAGCCGCTCGAGCAATTCGATCGGCGCTGTTTCATTGCCATGAATGCC‑GGCGGACAACAGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCACTTCGAgcgcg                                                                                                                                  <  8:34704/149‑1 (MQ=255)
              ggCCTGATCTTGCCGTTGGCCACGCCGTGCAGCAGCCGCTCGAGCAATTCGATCGGCGCTGTTTCATTGCCATGAATGCC‑GGCGGACAACAGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCGCTCGAAGTGCGcccggc                                                                                                                            <  5:144208/147‑4 (MQ=255)
              ggCCTGATCTTGCCGTTGGCCACGCCGTGCAGCAGCCGCTCGAGCAATTCGATCGGCGCTGTTTCATTGCCATGAATGCC‑GGCGGACAACAGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCACTTCGAGCGCGCCCTcc                                                                                                                            <  1:260121/147‑1 (MQ=255)
                 cTGATCTTGCCGTTGGCCACGCCGTGCAGCAGCCGCTCGAGCAATTCGATCGGCGCTGTTTCATTGCCATGAATGCC‑GGCGGACAACAGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAg                                                                                                                       >  5:19016/1‑149 (MQ=255)
                            gTTGGCCACGCCGTGCAGCAGCCGCTCGAGCAATTCGATCGGCGCTGTTTCATTGCCATGAATGCC‑GGCGGACAACAGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATc                                                                                                                  <  2:84518/143‑1 (MQ=255)
                            gTTGGCCACGCCGTGCAGCAGCCGCTCGAGCAATTCGATCGGCGCTGTTTCATTGCCATGAATGCC‑GGCGGACAACAGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATc                                                                                                                  >  1:79502/1‑143 (MQ=255)
                            gTTGGCCACGCCGTGCAGCAGCCGCTCGAGCAATTCGATCGGCGCTGTTTCATTGCCATGAATGCC‑GGCGGACAACAGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATc                                                                                                                  <  2:79502/143‑1 (MQ=255)
                            gTTGGCCACGCCGTGCAGCAGCCGCTCGAGCAATTCGATCGGCGCTGTTTCATTGCCATGAATGCC‑GGCGGACAACAGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAAc                                                                                                            <  3:113885/149‑1 (MQ=255)
                            gTTGGCCACGCCGTGCAGCAGCCGCTCGAGCAATTCGATCGGCGCTGATTCATTGCCATGAATGCC‑GGCGGACAACAGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATc                                                                                                                  >  1:84518/1‑143 (MQ=255)
                                gCCACGCCGTGCAGCAGCCGCTCGAGCAATTCGATCGGCGCTGTTTCATTGCCATGAATGCC‑GGCGGACAACAGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAAcgcg                                                                                                         <  5:8859/148‑1 (MQ=255)
                                  cACGCCGTGCAGCAGCCGCTCGAGCAATTCGATCGGCGCTGTTTCATTGCCATGAATGCC‑GGCGGACAACAGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCGCTCGAAGTGCGcccg                                                                                                                              <  2:228019/125‑2 (MQ=255)
                                  cACGCCGTGCAGCAGCCGCTCGAGCAATTCGATCGGCGCTGTTTCATTGCCATGAATGCC‑GGCGGACAACAGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCGCTCGAAGTGCGcccg                                                                                                                              >  1:228019/1‑124 (MQ=255)
                                  cACGCCGTGCAGCAGCCGCTCGAGCAATTCGATCGGCGCTGTTTCATTGCCATGAATGCC‑GGCGGACAACAGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACgcgcgc                                                                                                      >  4:274484/1‑149 (MQ=255)
                                    cGCCGTGCAGCAGCCGCTCGAGCAATTCGATCGGCGCTGTTTCATTGCCATGAATGCC‑GGCGGACAACAGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGccc                                                                                                    >  7:100342/1‑149 (MQ=255)
                                          gcagcaGCCGCTCGAGCAATTCGATCGGCGCTGTTTCATTGCCATGAATGCC‑GGCGGACAACAGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCACTTCGAgcgcgc                                                                                                                                 <  7:205586/114‑1 (MQ=255)
                                          gcagcaGCCGCTCGAGCAATTCGATCGGCGCTGTTTCATTGCCATGAATGCC‑GGCGGACAACAGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCACTTCGAgcgcgc                                                                                                                                 >  8:205586/1‑114 (MQ=255)
                                              cagcCGCTCGAGCAATTCGATCGGCGCTGTTTCATTGCCATGAATGCC‑GGCGGACAACAGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGGTGTCa                                                                                          <  1:361479/149‑1 (MQ=255)
                                                gcCGCTCGAGCAATTCGATCGGCGCTGTTTCATTGCCATGAATGCCTGGCGGACAACAGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGGTGTCAc                                                                                         >  1:261721/1‑149 (MQ=255)
                                                gcCGCTCGAGCAATTCGATCGGCGCTGTTTCATTGCCATGAATGCC‑GGCGGACAACAGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGGTGTCACt                                                                                        >  6:304603/1‑149 (MQ=255)
                                                    cTCGAGCAATTCGATCGGCGCTGTTTCATTGCCATGAATGCC‑GGCGGACAACAGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGGTGTCACTTggg                                                                                    >  8:103113/1‑149 (MQ=255)
                                                       gAGCAATTCGATCGGCGCTGTTTCATTGCCATGAATGCC‑GGCGGACAACAGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGGTGTCACTTGGGtct                                                                                 >  7:155403/1‑149 (MQ=255)
                                                       gAGCAATTCGATCGGCGCTGTTTCATTGCCATGAATGCC‑GGCGGACAACAGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGGTGTCACTTGGGtct                                                                                 >  6:292300/1‑149 (MQ=255)
                                                          cAATTCGATCGGCGCTGTTTCATTGCCATGAATGCC‑GGCGGACAACAGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGGTGTCACTTGGGtcttct                                                                              <  5:304603/149‑1 (MQ=255)
                                                            aTTCGATCGGCGCTGTTTCATTGCCATGAATGCC‑GGCGGACAACAGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGGTGTCACTTGGGTCTTCTCg                                                                            <  7:103113/149‑1 (MQ=255)
                                                             ttCGATCGGCGCTGTTTCATTGCCATGAATGCC‑GGCGGACAACAGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGGTGTCACTTGGGTCTTCTCgg                                                                           >  2:134314/1‑149 (MQ=255)
                                                               cGATCGGCGCTGTTTCATTGCCATGAATGCC‑GGCGGACAACAGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGGTGTCACTTGGGTCTTCTCGGc                                                                          <  1:223875/148‑1 (MQ=255)
                                                                gATCGGCGCTGTTTCATTGCCATGAATGCC‑GGCGGACAACAGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGGTGTCACTTGGGTCTTCTCGGCCg                                                                        >  7:280262/1‑149 (MQ=255)
                                                                           tttCATTGCCATGAATGCC‑GGCGGACAACAGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGGTGTCACTTGGGTCTTCTCGGCCGGTTCGTGAt                                                               <  8:155403/147‑1 (MQ=255)
                                                                           tttCATTGCCATGAATGCC‑GGCGGACAACAGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGGTGTCACTTGGGTCTTCTCGGCCGGTTCGTGATCg                                                             >  4:261851/1‑149 (MQ=255)
                                                                             tCAATGCCATGAATGCC‑GGCGGACAACAGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGGGGTCAGGTGGGGCGTCGCGGGGGggtcggggtcggg                                                           >  4:331828/1‑148 (MQ=255)
                                                                               aTTGCCATGAATGCC‑GGCGGACAACAGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGGTGTCACTTGGGTCTTCTCGGCCGGTTCGTGATCGGTGa                                                         <  1:134314/149‑1 (MQ=255)
                                                                                  gCCATGAATGCC‑GGCGGACAACAGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGGTGTCACTTGGGTCTTCTCGGCTGGTTCGTGATCGGTGAgg                                                       <  3:274484/148‑1 (MQ=255)
                                                                                  gCCATGAATGCC‑GGCGGACAACAGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGGTGTCACTTGGGTCTTCTCGGCCGGTTCGTGATCGGTGAgg                                                       <  8:100342/148‑1 (MQ=255)
                                                                                  gCCATGAATGCC‑GGCGGACAACAGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGGTGTCACTTGGGTCTTCTCGGCCGGTTCGTGATCGGTGAgg                                                       <  8:280262/148‑1 (MQ=255)
                                                                                   ccATGAATGCC‑GGCGGACAACAGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGGTGTCACTTGGGTCTTCTCGGCCGGTTCGTGATCGGTGAggg                                                      <  5:292300/148‑1 (MQ=255)
                                                                                   ccATGAATGCC‑GGCGGACAACAGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGGTGTCACTTGGGTCTTCTCGGCCGGTTCGTGATCGGTGAGGGt                                                     >  1:69209/1‑149 (MQ=255)
                                                                                    cATGAATGCC‑GGCGGACAACAGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGGTGTCACTTGGGTCTTCTCGGCCGGTTCGTGATCGGTGAGGGt                                                     >  5:226969/1‑148 (MQ=255)
                                                                                             c‑ggcggACAACAGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGGTGTCACTTGGGTCTTCTCGGCCGGTTCGTGATCGGTGAGGGTCAGCTCAAg                                            >  6:37639/1‑148 (MQ=255)
                                                                                                gcggACAACAGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGGTGTCACTTGGGTCTTCTCGGCCGGTTCGTGATCGGTGAGGGTCAGCTCAAGCaa                                         >  1:198988/1‑149 (MQ=255)
                                                                                                   gACAACAGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGGTGTCACTTGGGTCTTCTCGGCCGGTTCGTGATCGGTGAGGGTCAGCTCAAGCAAttt                                      <  2:69209/149‑1 (MQ=255)
                                                                                                      aacaGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGGTGTCACTTGGGTCTTCTCGGCCGGTTCGTGATCGGTGAGGGTCAGCTCAAGCAATTTGc                                    >  3:119191/1‑148 (MQ=255)
                                                                                                        caGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGGTGTCACTTGGGTCTTCTCGGCCGGTTCGTGATCGGTGAGGGTCAGCTCAAGCAATTTGcc                                   >  2:239584/1‑147 (MQ=255)
                                                                                                        caGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGGTGTCACTTGGGTCTTCTCGGCCGGTTCGTGATCGGTGAGGGTCAGCTCAAGCAATTTGCCaa                                 <  1:239584/149‑1 (MQ=255)
                                                                                                          gCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGGTGTCACTTGGGTCTTCTCGGCCGGTTCGTGATCGGTGAGGGTCAGCTCAAGCAATTTGCCAAgg                               <  7:313306/149‑1 (MQ=255)
                                                                                                                     ccGCAATCGTCGCTTTCGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGGTGTCACTTGGGTCTTCTCGGCCGGTTCGTGATCGGTGAGGGTCAGCTCAAGCAATTTGCCAAGGGCGAGCATAgg                    >  7:21651/1‑149 (MQ=255)
                                                                                                                      cGCAATCGTCGCTTTCGGCCGGGCTCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGGTGTCACTTGGGTCTTCTCGGCCGGTTCGTGATCGGTGAGTGTCAGCTCAAGCAATTTGCCAAGGGCGAGCATAGGt                   <  2:261721/149‑1 (MQ=255)
                                                                                                                       ccAATCGTCGCTTTCGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGGTGTCACTTGGGTCTTCTCGGCCGGTTCGTGATCGGTGAGTGTCAGCTCAAGCAATTTGCCAAGGGCGCGCATAGGTg                  >  2:246591/2‑149 (MQ=255)
                                                                                                                        cAATCGTCGCTTTCGGCCGGTCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGGTGTCACTTGGGTCTTCTCGGGCGGTTCGTGATCGGTGAGGGTCAGCTCAAGCAATTTGCCAAGGGCGAGCATAGGttc                 <  3:331828/149‑3 (MQ=255)
                                                                                                                             gtcgCTTTCGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGgtgt                                                                                            <  8:160451/69‑1 (MQ=255)
                                                                                                                             gtcgCTTTCGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGgtgt                                                                                            >  7:160451/1‑69 (MQ=255)
                                                                                                                               cgCTTTCGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGGTGTCACTTGGGTCTTCTCGGCCGGTTCGTGATCGGTGAGGGTCAGCTCAAGCAATTTGCCAAGGGCGAGCATAGGTGCTTCCTTa          <  7:8988/149‑1 (MQ=255)
                                                                                                                                    tCGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGGTGTCACTTGGGTCTTCTCGGCCGGTTCGTGATCGGTGAGGGTCAGCTCAAGCAATTTGCCAAGGGCGAGCATAGGTGCTTCCTTAgtg       <  4:171428/147‑1 (MQ=255)
                                                                                                                                     cGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGGTGTCACTTGGGTCTTCTCGGCCGGTTCGTGATCGGTGAGGGTCAGCTCAAGCAATTTGCCAAGGGCGAGCATAGGTGCTTCCTTAgtggt     <  2:198988/148‑1 (MQ=255)
                                                                                                                                      ggCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGGTGTCACTTGGGTCTTCTCGGCCGGTTCGTGATCGGTGAGGGTCAGCTCAAGCAATTTGCCAAGGGCGAGCATAGGTGCTTCCTTAgtggtg    <  2:194851/148‑1 (MQ=255)
                                                                                                                                      ggCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGGTGTCACTTGGGTCTTCTCGGCCGGTTCGTGATCGGTGAGGGTCAGCTCAAGCAATTTGCCAAGGGCGAGCATAGGTGCTTCCTTAgtggtg    <  3:261851/148‑1 (MQ=255)
                                                                                                                                      ggCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGGTGTCACTTGGGTCTTCTCGGCCGGTTCGTGATCGGTGAGGGTCAGCTCAAGCAATTTGCCAAGGGCGAGCATAGGTGCTTCCTTAgtggtg    <  4:329905/148‑1 (MQ=255)
                                                                                                                                       gCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGGTGTCACTTGGGTCTTCTCGGCCGGTTCGTGATCGGTGAGGGTCAGCTCAAGCAATTTGCCAAGGGCGAGCATAGGTGCTTCCTTAgtggtggt  <  2:108059/149‑1 (MQ=255)
                                                                                                                                       gCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGGTGTCACTTGGGGCTTCTCGGCCGGTTCGTGATCGGTGAGGGTCAGCTCAAGCAATTTGCCAAGGGCGAGCATAGGTGCTTCCTTAgtggtggt  <  6:347976/149‑1 (MQ=255)
                                                                                                                                              gcACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGGTGTCACTTGGGTCTTCTCGGCCGGTTCGTGATCGGTGAGGGTCAGCTCAAGCAATTTGcc                                   >  7:289111/1‑109 (MQ=255)
                                                                                                                                              gcACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGGTGTCACTTGGGTCTTCTCGGCCGGTTCGTGATCGGTGAGGGTCAGCTCAAGCAATTTGcc                                   <  8:289111/109‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                           
GCACCCGCGCCCTGGGCCTGATCTTGCCGTTGGCCACGCCGTGCAGCAGCCGCTCGAGCAATTCGATCGGCGCTGTTTCATTGCCATGAATGCC‑GGCGGACAACAGCAAGTCCAGCCCGCAATCGTCGCTTTCGGCCGGGCGCACTTCGAGCGCGCCCTCCCCCAGCCATCGCAAACGCGCGCCCTTGGGTGTCACTTGGGTCTTCTCGGCCGGTTCGTGATCGGTGAGGGTCAGCTCAAGCAATTTGCCAAGGGCGAGCATAGGTGCTTCCTTAGTGGTGGT  >  NC_002947/5085396‑5085678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑