Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,097,222	G→C	7.7%	intergenic (+213/+79)	PP_mr66 → / ← acsA‑I	P6/acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,097,222	0	G	C	7.7%	146.7 / 3.8	52	intergenic (+213/+79)	PP_mr66/acsA‑I	P6/acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base G (28/20);  new base C (2/2);  total (30/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.40e-01

CTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGC‑CCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGT  >  NC_002947/5097077‑5097362                                                                                                                                                   |                                                                                                                                              cTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGC‑CCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAgg                                                                                                                                            >  1:314065/1‑149 (MQ=255)     aTGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGC‑CCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAg                                                                                                                                        <  6:122838/149‑1 (MQ=255)     aTGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGC‑CCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAg                                                                                                                                        <  5:142798/149‑1 (MQ=255)       gCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGC‑CCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAggg                                                                                                                                      >  7:176844/1‑149 (MQ=255)        cacaAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGC‑CCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAggg                                                                                                                                      >  3:229352/1‑148 (MQ=255)           aaaaTGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCACCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAggct                                                                                                                                     >  1:89798/1‑145 (MQ=255)                    aCTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGC‑CCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGc                                                                                                                          <  2:270535/147‑1 (MQ=255)                     cTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGC‑CCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCt                                                                                                                        >  4:5089/1‑148 (MQ=255)                      tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGC‑CCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTg                                                                                                                       >  6:163646/1‑148 (MQ=255)                        cGATGCACCGAAACGGGCCTGAATTGTTTCCTGGCGC‑CCCGAACCTGTGCGGGGCCGGTGACAGACCAGTAACAGATGATTTTCGGGTAACAATGCAAAAGCGGGCTTGCCCGAGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTTaa                                                                                                                     <  7:291728/148‑1 (MQ=255)                        cGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGC‑CGCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAAc                                                                                                                    <  3:315138/149‑1 (MQ=255)                          aTGCACCGAAACGGGCCGGAATTGTTTCCTGGCGC‑CCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTg                                                                                                                  <  4:229352/149‑1 (MQ=255)                           tGCACCGAAACGGGCCGGAATTGTTTCCTGGCGC‑CCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAACGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGa                                                                                                                 <  5:218080/149‑1 (MQ=255)                             cACCGAAACGGGCCGGAATTGTTTCCTGGCGC‑CCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                <  3:5089/148‑1 (MQ=255)                             cACCGAAACGGGCCGGAATTGGTTCCTGGCGC‑CCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCTGGTAACAATGCAAAAGCGGGCTTGCCCGCTAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                <  2:223014/148‑1 (MQ=255)                                    aCGGGCCGGAATTGTTTCCTGGCGC‑CCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTcgc                                                                                                        <  1:101411/149‑1 (MQ=255)                                    aCGGGCCGGAATTGTTTCCTGGCGC‑CCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTcgc                                                                                                        <  8:215242/149‑1 (MQ=21)                                     cGGGCCGGAATTGTTTCCTGGCGC‑CCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTcgcg                                                                                                       <  5:283636/149‑1 (MQ=255)                                       ggCCGGAATTGTTTCCTGGCGC‑CCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCggg                                                                                                     <  4:216542/149‑1 (MQ=255)                                         ccGGAATTGTTTCCTGGCGC‑CCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGAGCGGTCAGGGGACAGCT‑TAGCCTGAACAGACGCATTAGCGGGc                                                                                                    >  4:317364/1‑148 (MQ=255)                                                 gTTTCCTGGCGC‑CCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTTCCCGCGCATGCGTGAGGTGAGGGGACAGCT‑TAGCCTGAACTGACGCATTAGAGGGCAAGCCCCCt                                                                                           >  1:123046/1‑149 (MQ=255)                                                  tttCCTGGCGC‑CCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTAGCCGGCAAGCCCGCTc                                                                                          >  1:244824/1‑149 (MQ=255)                                                       accggc‑CCCGCACAGGTTCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCA‑TAGCCTGAACTGACGCCTTCGCGGGCAAGCCCGCTCCTg                                                                                       >  5:311789/5‑147 (MQ=255)                                                           gc‑CCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTGCCCGCGAATGGGGGCGGTGAGGGGACAGCG‑TTGCCTGTACTGACGCATTAGCGGGCAACCCCGCGCCTGCAgggg                                                                                 >  1:126391/1‑149 (MQ=255)                                                               ccGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAAc                                                                                                                    <  1:166981/111‑1 (MQ=255)                                                               ccGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAAc                                                                                                                    >  2:166981/1‑111 (MQ=255)                                                                         gCGGGGCCGGGGACAGAACTGTAACCGATGATTGGCGGGAAACAATGCAAACGCGGGCTGGCCCGCTAATGCGTGAGGGCAGGGCACCGCT‑AAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGaa                                                                    <  5:163646/149‑1 (MQ=255)                                                                                         aaCAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGGCAGCT‑TAGCCTGAACTGACGCATTAGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGc                                                    >  3:64409/1‑149 (MQ=255)                                                                                         aaCAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCCCATGCGTGAGGGGAGGGGGCAGCA‑TTGCCAGAACTGACGCAATAGCGGGCCACCCCCCCCCAGGAGGGGGGTTTTGCGGGAAGGGTTTAAGCCGAAGc                                                    >  4:176216/1‑149 (MQ=255)                                                                                              tAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTTGCCGCGAATGCGGGAGGTCAGGGGGCAGCT‑TAGCCGGAACTGACCCATTCCCGGGCAAGCCCGCGCCGGCAGGGGGGATTGCCGGGAAGGGTTTACCCCGAAGCCACGt                                               >  3:41879/1‑149 (MQ=255)                                                                                              tAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGt                                               >  5:298983/1‑149 (MQ=255)                                                                                               aaCCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGcc                                                   >  5:150558/1‑144 (MQ=255)                                                                                                 ccGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTAGCGGGCAAGCCCGCTCCTGCAGGGGGTTTTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCa                                            >  5:116352/1‑149 (MQ=255)                                                                                                  cGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGCATGGGTGAGGTCAGGGGCCAGCG‑TAGCCAGAAAAGACGCATTCGCCGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCAt                                           >  3:143071/1‑149 (MQ=255)                                                                                                            gggtaaaCAAAGAAAACGCGGGCTGGCCCGCTAATGCGTGCGGACCGGGCACAGCT‑TCGCCTGAACTGACGATTTCGGGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTgg                                 <  7:153120/144‑1 (MQ=255)                                                                                                             gtgTAACAATGCAAACGCGGGCTTGCCCGCGAATGCGTGAGGGCCTGGCACAGCT‑TAGCCGGAACTGCCGCATTCGGGGGAAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTgg                                 <  8:176844/146‑1 (MQ=255)                                                                                                                    aaTGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTgg                                 <  1:50721/141‑1 (MQ=255)                                                                                                                    aaTGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTgg                                 >  2:50721/1‑141 (MQ=255)                                                                                                                      tGCAAAAGCGGGCTTGCCCGCGAAAGCGTGAGGTCAGGGGACAGCG‑TAGCCTGAACAGACGCATTAGCGGGCAAGCCCGCTCCTGCAGGGGGTAATGCCGGGAATGGTTTTCGCCGAAGCCAGGTTCATGGCCGTGTGGGTATGGAt                         >  8:208638/1‑147 (MQ=255)                                                                                                                         aaaaGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGt                    >  2:4505/1‑149 (MQ=255)                                                                                                                         aaaaGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGt                    >  5:79636/1‑149 (MQ=255)                                                                                                                               gggTTTGCCGGCTATTGGGTGAGGTCAGGGGCATTTT‑TTGCCTTAACTGAGGCTTTCGGGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTa              <  4:64409/149‑1 (MQ=255)                                                                                                                               gggCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATc                        >  6:145526/1‑139 (MQ=255)                                                                                                                               gggCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATc                        <  5:145526/139‑1 (MQ=255)                                                                                                                                       ccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACcac          >  1:21576/1‑145 (MQ=255)                                                                                                                                       ccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACcac          <  2:21576/145‑1 (MQ=255)                                                                                                                                       ccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc      >  7:220986/1‑149 (MQ=255)                                                                                                                                        cgcgAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGCGCAAGCCCGCTCCTGCAGGGGGTATTCCCGGGAAGGGTTTACGCCGAAGCCAGCTTCATCGCCTAGAGGGTATCGATCAGGTGCTGTACCACACCCg     >  6:170238/1‑149 (MQ=255)                                                                                                                                         gcgAATGCGTGAGGTCAGGGGCCAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCAGCGCCTTGGGGGTATCGATCAGGTGCTGTCCCACACCCgg    >  4:343762/1‑149 (MQ=255)                                                                                                                                         gcgAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATc                                          >  2:322819/1‑111 (MQ=14)                                                                                                                                             aTGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGt  >  8:222478/1‑147 (MQ=255)                                                                                                                                             aTGCGGGAGGTCCGGGTACTGTT‑TACCCGTAACTCACGCATGCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGt  <  6:79636/147‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                              CTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGC‑CCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGT  >  NC_002947/5097077‑5097362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑