Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,108,774 A→G 5.5% I171V (ATC→GTC)  PP_4494 → LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,108,7740AG 5.5% 215.0 / 2.1 72I171V (ATC→GTC) PP_4494LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (36/32);  new base G (2/2);  total (38/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

CTCACCCGGGCATGCTGGTGCACATTCATTCACGCATTGAAACCATCAACTTCGACACCAGCGAAATCGATGCCGCCATCGGCGTGGCCAGCCACGACTTGCCGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTGGAGCCCGGCCAGGATCAGCGAAGAGGTGCTGCTGAATGTGGCCAACAACCCGCATGCGTGGGGCGAGTGGTTTTCCCATCA‑TGGCCTGCCGCACCGGGCGATGCGGCTGGG  >  NC_002947/5108628‑5108922
                                                                                                                                                  |                                                                                                                                                     
cTCACCCGGGCATGCTGGTGCACATTCATTCACGCATTGAAACCATCAACTTCGACACCAGCGAAATCGATGCCGCCATCGGCGTGGCCAGCCACGACTTGCCGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATc                                                                                                                                                     >  3:338789/1‑149 (MQ=255)
   aCCCGGGCATGCTGGTGCACATTCATTCACGCATTGAAACCATCAACTTCGACACCAGCGAAATCGATGCCGCCATCGGCGTGGCCAGCCACGACTTGCCGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTg                                                                                                                                                  <  5:341814/149‑1 (MQ=255)
    cccGGGCATGCTGGTGCACATTCATTCACGCATTGAAACCATCAACTTCGACACCAGCGAAATCGATGCCGCCATCGGCGTGGCCAGCCACGACTTGCCGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTgc                                                                                                                                                 <  6:27312/149‑1 (MQ=255)
             gCTGGTGCACATTCATTCACGCATTGAAACCATCAACTTCGACACCAGCGAAATCGATGCCGCCATCGGCGTGGCCAGCCACGACTTGCCGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAgg                                                                                                                                        >  5:94871/1‑149 (MQ=255)
                ggTGCACATTCATTCACGCATTGAAACCATCAACTTCGACACCAGCGAAATCGATGCCGCCATCGGCGTGGCCAGCCACGACTTGCCGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTg                                                                                                                                     >  4:128240/1‑149 (MQ=255)
                 gTGCACATTCATTCACGCATTGAAACCATCAACTTCGACACCAGCGAAATCGATGCCGCCATCGGCGTGGCCAGCCACGACTTGCCGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGGTCTTGCCGCCGGAGGCTgg                                                                                                                                    >  2:186982/1‑149 (MQ=255)
                 gTGCACATTCATTCACGCATTGAAACCATCAACTTCGACACCAGCGAAATCGATGCCGCCATCGGCGTGGCCAGCCACGACTTGCCGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGGTCTTGCCGCCGGAGGCTgg                                                                                                                                    >  2:211218/1‑149 (MQ=255)
                 gTGCACATTCATTCACGCATTGAAACCATCAACTTCGACACCAGCGAAATCGATGCCGCCATCGGCGTGGCCAGCCACGACTTGCCGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTgg                                                                                                                                    >  4:209011/1‑149 (MQ=255)
                  tGCACAGTCATTCACGCATTGAAACCATCCACTTCGACCCCAGCGAAATCGATGCCGCCATCGGCGGGGCCAGCCACGACTTGCCGGGTCTTGTCTGCCATCGCCTGCATGACGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGc                                                                                                                                   <  3:103135/149‑1 (MQ=255)
                       attcattcACGCATTGAAACCATCAACTTCGACACCAGCGAAATCGATGCCGCCATCGGCGTGGCCAGCCACGACTTGCCGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCgg                                                                                                                              >  6:360286/1‑149 (MQ=255)
                       attcattcACGCATTGAAACCATCAACTTCGACACCAGCGAAATCGATGCCGCCATCGGCGTGGCCAGCCACGACTTGCCGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCgg                                                                                                                              <  3:128240/149‑1 (MQ=255)
                         tcattcaCGCATTGAAACCATCAACTTCGACACCAGCGAAATCGATGCCGCCATCGGCGTGGCCAGCCACGACTTGCCGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCgg                                                                                                                              >  4:213538/1‑147 (MQ=255)
                           attcaCGCATTGAAACCATCAACTTCGACACCAGCGAAATCGATGCCGCCATCGGCGTGGCCAGCCACGACTTGCCGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCtt                                                                                                                                                   <  2:3704/124‑1 (MQ=255)
                           attcaCGCATTGAAACCATCAACTTCGACACCAGCGAAATCGATGCCGCCATCGGCGTGGCCAGCCACGACTTGCCGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCtt                                                                                                                                                   >  1:3704/1‑124 (MQ=255)
                           attcaCGCATTGAAACCATCAACTTCGACACCAGCGAAATCGATGCCGCCATCGGCGTGGCCAGCCACGACTTGCCGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTc                                                                                                                                >  7:81355/1‑143 (MQ=255)
                           attcaCGCATTGAAACCATCAACTTCGACACCAGCGAAATCGATGCCGCCATCGGCGTGGCCAGCCACGACTTGCCGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTc                                                                                                                                <  8:81355/143‑1 (MQ=255)
                           attatcGCATTGAAACCATCAACTTCGAGACCAGCGAAATCGATGCCGCCATGGGCGTGGCCAGCCAGGACTTGCCGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCAg                                                                                                                           <  6:233208/143‑1 (MQ=255)
                            ttcaCGCATTGAAACCATCAACTTCGACACCAGCGAAATCGATGCCGCCATCGGCGTGGCCAGCCACGACTTGCCGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCAGc                                                                                                                          >  6:88249/1‑148 (MQ=255)
                               aCGCATTGAAACCATCAACTTCGACACCAGCGAAATCGATGCCGCCATCGGCGTGGCCAGCCACGACTTGCCGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAg                                                                                                                      >  7:325891/1‑149 (MQ=255)
                               aCGCATTGAAACCATCAACTTCGACACCAGCGAAATCGATGCCGCCATCGGCGTGGCCAGCCACGACTTGCCGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAg                                                                                                                      >  6:160867/1‑149 (MQ=255)
                               aCGCATTGAAACCATCAACTTCGACACCAGCGAAATCGATGCCGCCATCGGCGTGGCCAGCCACGACTTGCCGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGCCGACGCCAGcctcc                                                                                                                      >  3:318894/1‑146 (MQ=255)
                               aCGCATTGAAACCATCAACTTCGACACCAGCGAAATCGATGCCGCCATCGGCGTGGCCAGCCACGACTTGCCGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGCCGACGCCAGcctcc                                                                                                                      <  4:318894/149‑4 (MQ=255)
                                 gCATTGAAACCATCAACTTCGACACCAGCGAAATCGATGCCGCCATCGGCGTGGCCAGCCACGACTTGCCGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGCCGACGCCAGcctccgg                                                                                                                    <  6:129909/149‑6 (MQ=255)
                                      gAAACCATCAACTTCGACACCAGCGAAATCGATGCCGCCATCGGCGTGGCCAGCCACGACTTGCCGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTGGa                                                                                                                <  5:360286/148‑1 (MQ=255)
                                        aaCCATCAACTTCGACACCAGCGAAATCGATGCCGCCATCGGCGTGGCCAGCCACGACTTGCCGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTGGAGcc                                                                                                             >  3:213472/1‑149 (MQ=255)
                                        aaCCATCAACTTCGACACCAGCGAAATCGATGCCGCCATCGGCGTGGCCAGCCACGACTTGCCGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGATGGAGcc                                                                                                             >  8:253421/1‑149 (MQ=255)
                                         aCCATCAACTTCGACACCAGCGAAATCGATGCCGCCATCGGCGTGGCCAGCCACGACTTGCCGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTg                                                                                                                  >  2:87761/1‑143 (MQ=255)
                                         aCCATCAACTTCGACACCAGCGAAATCGATGCCGCCATCGGCGTGGCCAGCCACGACTTGCCGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTg                                                                                                                  <  1:87761/143‑1 (MQ=255)
                                          ccATCAACTTCGACACCAGCGAAATCCATGCCGCCATCGGCGTGGCCAGCCACGACTTGCCGGGTCTTGTCTGCCATCGCCTGGATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTGGAGCCCg                                                                                                           >  5:329948/1‑149 (MQ=255)
                                              cAACTTCGACACCAGCGAAATCGATGCCGCCATCGGCGTGGCCAGCCACGACTTGCCGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTGGAGCCCGGCCa                                                                                                       <  1:15685/149‑1 (MQ=255)
                                                  ttCGACACCAGCGAAATCGATGCCGCCATCGGCGTGGCCAGCCACGACTTGCCGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTGGAGCCCGGCCacatt                                                                                                   >  1:59158/1‑145 (MQ=255)
                                                  ttCGACACCAGCGAAATCGATGCCGCCATCGGCGTGGCCAGCCACGACTTGCCGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTGGAGCCCGGCCAGGAt                                                                                                   >  7:32969/1‑149 (MQ=255)
                                                      acacCAGCGAAATCGATGCCGCCATCGGCGTGGCCAGCCACGACTTGCCGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTGGAGCCCGGCCAGGATCAGc                                                                                               <  3:213538/149‑1 (MQ=255)
                                                        acCAGCGAAATCGATGCCGCCATCGGCGTGGCCAGCCACGACTTGCCGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCa                                                                                                                            <  5:75449/118‑1 (MQ=255)
                                                        acCAGCGAAATCGATGCCGCCATCGGCGTGGCCAGCCACGACTTGCCGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCa                                                                                                                            >  6:75449/1‑118 (MQ=255)
                                                          cAGCGAAATCGATGCCGCCATCGGCGTGGCCAGCCACGACTTGCCGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTGGAGCCCGGCCAGGATCAGCGAag                                                                                           <  5:222405/149‑1 (MQ=255)
                                                           aGCGAAATCGATGCCGCCATCGGCGTGGCCAGCCACGACTTGCCGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTGGAGCCCGGCCAGGATCAGCGAaga                                                                                          <  1:353870/149‑1 (MQ=255)
                                                               aaaTCGATGCCGCCATCGGCGTGGCCAGCCACGACTTGCCGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTGGAGCCCGGCCAGGATCAGCGAAGAGGtg                                                                                      <  8:325891/149‑1 (MQ=255)
                                                                aaTCGATGCCGCCATCGGCGTGGCCAGCCACGACTTGCGGCGGCTTGTCTGCAATCGCCTGCCTGCCGCGGAGCTGGTGGTGATCTTGCCGCCGGACGATGGCGTCGGCAGCCAAGGCTGGAGCCCGGCCAGGATCCGGGACGAGCtgc                                                                                     >  5:72216/1‑149 (MQ=255)
                                                                 aTCGATGCCGCCATCGGCGTGGCCAGCCACGACTTGCCGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTGGAGCCCGGCCAGGATCAGCGAAGAGGtgct                                                                                    <  8:339420/149‑1 (MQ=255)
                                                                    gATGCCGCCATCGGCGTGGCCAGCCACGACTTGCCGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTGGAGCCCGGCCAGGATCAGCGAAGAGGTgctgct                                                                                 <  4:338789/149‑1 (MQ=255)
                                                                     aTGCCGCCATCGGCGTGGCCAGCCACGAATTGCCGGGTCTTGTCTCCCATCGCCTGCATGCCGAGGAGCTGGTTGTGATCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTGGAGCCCGGCCAGGATCATCGAAGAGGTgctgct                                                                                 >  4:288597/1‑148 (MQ=255)
                                                                      tGCCGCCATCGGCGTGGCCAGCCACGACTTGCCGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTGGAGCCCGGCCAGGATCAGCGAAGAGGTGCTGCTGa                                                                               <  3:209011/149‑1 (MQ=255)
                                                                          gccATCGGCGTGGCCAGCCACGACTTGCCGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTGGAGCCCGGCCAGGATCAGCGAAGAGGTGCTGCTGAAtgt                                                                           <  5:160867/149‑1 (MQ=255)
                                                                                       ccagccaCGACTTGCCGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTGGAGCCCGGCCAGGATCAGCGAAGAGGTGCTGCTGAATGTGGCCAACAACCCg                                                              <  4:213472/149‑1 (MQ=255)
                                                                                           ccaCGACTTGCCGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTGGAGCCCGGCCAGGATCAGCGAAGAGGTGCTGCTGAATGTGGCCAACAACCCGCATg                                                          >  8:72441/1‑149 (MQ=255)
                                                                                                  ttGCCGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTGGAGCCCGGCCAGGATCAGCGAAGAGGTGCTGCTGAATGTGGCCAACAACCCGCATGCGTgggg                                                   >  2:26795/1‑149 (MQ=255)
                                                                                                     ccGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTGGAGCCCGGCCAGGATCAGCGAAGAGGTGCTGCTGAATGTGGCCAACAACCCGCATGCGTGGGGCGa                                                <  7:72441/149‑1 (MQ=255)
                                                                                                       gggTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTGGAGCCCGGCCAGGATCAGCGAAGAGGTGCTGCTGAATGTGGCCAACAACCCGCATGCGTGGGGc                                                  >  5:48399/1‑145 (MQ=255)
                                                                                                       gggTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTGGAGCCCGGCCAGGATCAGCGAAGAGGTGCTGCTGAATGTGGCCAACAACCCGCATGCGTGGGGc                                                  <  6:48399/145‑1 (MQ=255)
                                                                                                       gggTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGCGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTGGAGCCCGGCCAGGATCAGCGAAGAGGTGCTGCTGAATGTGGCCAACAACCCGCATGCGTGGGGCGAGt                                              >  3:53325/1‑149 (MQ=255)
                                                                                                        ggTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCAGc                                                                                                                          >  3:263253/1‑72 (MQ=255)
                                                                                                        ggTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCAGc                                                                                                                          <  4:263253/72‑1 (MQ=255)
                                                                                                        ggTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTGGAGCCCGGCCAGGATCAGCGAAGAGGTGCTGCTGAATGTGGCCAACAACCCGCATGCGTGGGGCGAGTg                                             <  8:32969/149‑1 (MQ=255)
                                                                                                        ggTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTGGAGCCCGGCAAGGATCAGCGAAGAGGTGCTGCTGAATGTCGCCAACCACCCGCATGCGTGGGGCGAGTg                                             >  4:210481/1‑149 (MQ=255)
                                                                                                            ttGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTGGAGCCCGGCCAGGATCAGCGAAGAGGTGCTGCTGAATGTGGCCAACAACCCGCATGCGTGGGGCGAGTGGgtt                                         >  7:286339/1‑149 (MQ=255)
                                                                                                              gTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTGGAGCCCGGCCAGGATCAGCGAAGAGGTGCTGCTGAATGTGGCCAACAAccc                                                               <  5:261057/125‑1 (MQ=255)
                                                                                                              gTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTGGAGCCCGGCCAGGATCAGCGAAGAGGTGCTGCTGAATGTGGCCAACAAccc                                                               >  6:261057/1‑125 (MQ=255)
                                                                                                              gTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTGGAGCCCGGCCAGGATCAGCGAAGAGGTGCTGCTGAATGTGGCCAACAAGCCGCATGCGTGGGGCGAGTGGtttt                                        <  6:94871/148‑1 (MQ=255)
                                                                                                                 tGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATGTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTGGAGCCCGGCCAGGATCAGCTAAGAGGTGCTGCTGAATGTGGCCAACAACCCGCTTGCGTGGGGCGAGTGGTTTTCCca                                    <  3:210481/149‑1 (MQ=255)
                                                                                                                   ccATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTGGAGCCCGGCCAGGATCAGCGAAGAGGTGCTGCTGAATGTGGCCAACAACCCGCATGCGTGGGGCGAGTGGTTTTCCcat                                   >  5:366797/1‑148 (MQ=255)
                                                                                                                    cATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTGGAGCCCGGCCAGGATCAGCGAAGAGGTGCTGCTGAATGTGGCCAACAACCCGCATGCGTGGGGCGAGTGGTTTTCCcatca                                 >  7:106444/1‑149 (MQ=255)
                                                                                                                     aTCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTGGAGCCCGGCCAGGATCAGCGAAGAGGTGCTGCTGAATGTGGCCAACAACCCGCATGCGt                                                       >  3:357481/1‑126 (MQ=255)
                                                                                                                     aTCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTGGAGCCCGGCCAGGATCAGCGAAGAGGTGCTGCTGAATGTGGCCAACAACCCGCATGCGt                                                       <  4:357481/126‑1 (MQ=255)
                                                                                                                        gCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTGGAGCCCGGCCAGGATCAGCGAAGAGGTGCTGCTGAATGTGGCCAACAACCCGCATGCGTGGGGCGAGTGGTTTTCCCATCA‑TGGc                            <  5:88249/149‑1 (MQ=255)
                                                                                                                                    aggagCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTGGAGCCCGGCCAGGATCAGCGAAGAGGTGCTGCTGAATGTGGCCAACAACCCGCATGCGTGGGGCGAGTGGTTTTCCCATCA‑TGGCCTGCCGCACCgg                >  2:326039/1‑149 (MQ=255)
                                                                                                                                            gtggtgATCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTGGAGCCCGGCCAGGATCAGCGAAGAGGTGCTGCTGAATGTGGCCAACAACCCGCATGCGTGGGGCGAGTGGTTTTCCCATCA‑TGGCCTGCCGCACCGGGCGATGCg        >  3:31684/1‑149 (MQ=255)
                                                                                                                                              ggtgATCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTGGAGCCCGGCCAGGATCAGCGAAGAGGTGCTGCTGAATGTGGCCAACAACCCGCATGCGTGGGGCGAGTGGtttt                                        >  4:249243/1‑116 (MQ=255)
                                                                                                                                              ggtgATCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTGGAGCCCGGCCAGGATCAGCGAAGAGGTGCTGCTGAATGTGGCCAACAACCCGCATGCGTGGGGCGAGTGGtttt                                        <  3:249243/116‑1 (MQ=255)
                                                                                                                                               gtgGTCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTGGAGCCCGGCCAGGATCAGCGAAGAGGTGCTGCTGAATGTGGCCAACAACCCGCATGCGTGGGGCGAGTGGTTTTCCCATCATTGGCCTGCCGCACCGGGCGATGCGGc      <  1:186982/149‑1 (MQ=255)
                                                                                                                                               gtgGTCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTGGAGCCCGGCCAGGATCAGCGAAGAGGTGCTGCTGAATGTGGCCAACAACCCGCATGCGTGGGGCGAGTGGTTTTCCCATCA‑TGGCCTGCCGCACCGGGCGATGCGGc      <  1:211218/148‑1 (MQ=255)
                                                                                                                                               gtgATCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTGGAGCCCGGCCAGGATCAGCGAAGAGGTGCTGCTGAATGTGGCCAACAACCCGCATGCGTGGGGCGAGTGGTTTTCCCATCA‑TGGCCTGCCGCACCGGGCGATGCGGCt     >  3:241114/1‑149 (MQ=255)
                                                                                                                                                  aTCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTGGAGCCAGGCCAGGATCAGCGAAGAGGTGCTGCTGAATGTGGCCAACAACCCGCATGCGTCGGGCGACTGGTTTTCCCATCA‑TGGCCTGCCGCACCGGGCGATGCCGCTggg  >  8:131852/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
CTCACCCGGGCATGCTGGTGCACATTCATTCACGCATTGAAACCATCAACTTCGACACCAGCGAAATCGATGCCGCCATCGGCGTGGCCAGCCACGACTTGCCGGGTCTTGTCTGCCATCGCCTGCATGCCGAGGAGCTGGTGGTGATCTTGCCGCCGGAGGCTGGCGTCGGCAGCCAAGGCTGGAGCCCGGCCAGGATCAGCGAAGAGGTGCTGCTGAATGTGGCCAACAACCCGCATGCGTGGGGCGAGTGGTTTTCCCATCA‑TGGCCTGCCGCACCGGGCGATGCGGCTGGG  >  NC_002947/5108628‑5108922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑