Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,162,834 T→C 12.8% A339A (GCA→GCG)  PP_4545 ← beta‑ketodecanoyl‑ACP synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,162,8340TC12.8% 89.7 / 5.8 39A339A (GCA→GCG) PP_4545beta‑ketodecanoyl‑ACP synthase
Reads supporting (aligned to +/- strand):  ref base T (14/20);  new base C (2/3);  total (16/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.02e-01

CACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAGGTCATCCTGGTACAGGTGGAAGGCAATCACCGACCCCGCCGAGCTGGTATTGGCGTAACGGTCCAGAATCACCGGTGCCTCTTCCTCGGCCACTTCACGCCCCAACAGTTTCTTGACGATCAGCTGGTTCATGCTGAGGTTGGCCTGGTGCAGCCAGAAGCGCTTCACGTCGCCTGGTTGCAACCCGTTCTCCTGCAGGTGCTCAC  >  NC_002947/5162696‑5162964
                                                                                                                                          |                                                                                                                                  
cacaCTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAGGTCATCCTGGTACAGGTGGAAGGCAATCACCGACCCCGCCGAGCTGGTATTGGCGTAACGGTCCAGAATCACCGGTGCCTCTTCCt                                                                                                                          >  3:240482/1‑149 (MQ=255)
 acacTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAGGTCATCCTGGTACAGGTGGAAGGCAATCACCGACCCCGCCGAGCTGGTATTGGCGTAACGGTCCAGAATCACCGGTGCCTCTTCCTc                                                                                                                         >  1:250859/1‑149 (MQ=255)
    cTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAGGTCATCCTGGTACAGGTGGAAGGCAATCACCGACCCCGCCGAGCTGGTATTGGCGTAACGGTCCAGAATCACCGGTGCCTCTTCCTCGGc                                                                                                                      <  8:263743/149‑1 (MQ=255)
     tACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAGGTCATCCTGGTACAGGTGGAAGGCAATCACCGACCCCGCCGAGCTGGTATTGGCGTAACGGTCCAGAATCACCGGt                                                                                                                                    <  1:147993/134‑1 (MQ=255)
     tACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAGGTCATCCTGGTACAGGTGGAAGGCAATCACCGACCCCGCCGAGCTGGTATTGGCGTAACGGTCCAGAATCACCGGt                                                                                                                                    >  2:147993/1‑134 (MQ=255)
         aaTCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAGGTCATCCTGGTACAGGTGGAAGGCAATCACCGACCCCGCCGAGCTGGTATTGGCGTAACGGTCCAGAATCACCGGTGCCTCTTCCTCGGCCACtt                                                                                                                 >  2:71028/1‑149 (MQ=255)
          aTCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAGGTCATCCTGGTACAGGTGGAAGGCAATCACCGACCCCGCCGAGCTGGTATTGGCGTAACGGTCCAGAATCACCGGTGCCTCTTCCTCGGCCACTTc                                                                                                                <  6:156388/149‑1 (MQ=255)
              aaTACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAGGTCATCCTGGTACAGGTGGAAGGCAATCACCGACCCCGCCGAGCTGGTATTGGCGTAACGGTCCAGAATCACCGGTGCCTCTTCCTCGGCCACTTCACGc                                                                                                            >  6:140131/1‑149 (MQ=255)
              aaTACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAGGTCATCCTGGTACAGGTGGAAGGCAATCACCGACCCCGCCGAGCTGGTATTGGCGTAACGGTCCAGAATCACCGGTGCCTCTTCCTCGGCCACTTCACGc                                                                                                            >  6:46636/1‑149 (MQ=255)
               aTACCCCACACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAGGTCATCCTGGTACAGGTGGAAGGCAATCACCGACCCCGCCGAGCTGGTATTGGCGTAACGGTCCAGAATCACCGGTGCCTCTTCCTCGGCCACTTCACGcc                                                                                                           <  2:298393/149‑1 (MQ=255)
                  ccccGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAGGTCATCCTGGTACAGGTGGAAGGCAATCACCGACCCCGCCGAGCTGGTATTGGCGTAACGGTCCAGAATCACCGGTGCCTCTTCCTCGGCCACTTCACGcccc                                                                                                         <  5:140131/148‑1 (MQ=255)
                     cGCTCCAAACGAGCTGAACACGCCCAACGAGCCTTTGGCCAGGTCATCCTGGTACAGGTGGAAGGCAATCACCGACCCCGCCGAGCTGGTATTGGCGTAACGGTCCAGAATCACCGGCGCCTCTTCCTCGGCCACTTCACGCCCCAACa                                                                                                     <  2:269620/149‑1 (MQ=255)
                        aCCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAGGTCATCCTGGTACAGGTGGAAGGCAATCACCGACCCCGCCGAGCTGGTATTGGCGTAACGGTCCAGAATCACCGGTGCCTCTTCCTCGGc                                                                                                                      <  2:318200/129‑1 (MQ=255)
                        aCCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAGGTCATCCTGGTACAGGTGGAAGGCAATCACCGACCCCGCCGAGCTGGTATTGGCGTAACGGTCCAGAATCACCGGTGCCTCTTCCTCGGc                                                                                                                      >  1:318200/1‑129 (MQ=255)
                          cAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAGGTCATCCTGGTACAGGTGGAAGGCAATCACCGACCCCGCCGAGCTGGTATTGGCGTAACGGTCCAGAATCACCGGTGCCTCTTCCTCGGCCACTTCACGCCCCAACAGTTTc                                                                                                <  1:71028/149‑1 (MQ=255)
                              cGAGCTCAACACGCCCAACGAGCCTTTGGCCAGGTCATCCTGGTACAGGTGGAAGGCAATCACCGACCCCGCCGAGCTGGTATTGGCGTAACGGTCCAGAATCACCGGTGCCTCTTCCTCGGCCACTTCACGCCCCAACAGTTTCTTGa                                                                                            <  5:46636/149‑1 (MQ=255)
                              cGAGCTCAACACGCCCAACGAGCCTTTGGCCAGGTCATCCTGGTACAGGTGGAAGGCAATCACCGACCCCGCCGAGCTGGTATTGGCGTAACGGTCCAGAATCACCGGTGCCTCTTCCTCGGCCACTTCACGCCCCAACAGTTTCTTGa                                                                                            <  5:107672/149‑1 (MQ=255)
                                   tCAACACGCCCAACGAGCCTTTGGCCAGGTCATCCTGGTACAGGTGGAAGGCAATCACCGACCCCGCCGAGCTGGTATTGGCGTAACGGTCCAGAATCACCGGCGCCTCTTCCTCGGCCACTTCACGCCCCAACAGTTTCTTGACGAt                                                                                        >  4:247411/1‑148 (MQ=255)
                                               aCGAGCCTTTGGCCAGGTCATCCTGGGACTGGTGGAAGGCAATCACCGACCCCGCCGAGCTGGTATTGGCGTAACGGTCCAGAATCACCGGTGCCTCTTCCTCGGCCACTTCACGCCCCAACAGTTTCTTGACGATCAGCTGGTTCATg                                                                           <  7:329227/149‑1 (MQ=255)
                                                 gAGCCTTTGGCCAGGTCATCCTGGTACAGGTGGAAGGCAATCACCGACCCCGCCGAGCTGGTATTGGCGTAACGGTCCAGAATCACCGGTGCCTCTTCCTCGGCCACTTCACGCCCCAACAGTTTCTTGACGATCAGCTGGTTCATGCt                                                                         <  2:250859/149‑1 (MQ=255)
                                                               gTCATCCTGGTACAGGTGGAAGGCAATCACCGACCCCGCCGAGCTGGTATTGGCGTAACGGTCCAGAATCACCGGTGCCTCTTCCTCGGCCACTTCACGCCCCAACAGTTTCTTGACGATCAGCTGGTTCATGCTGAGGTTGGCCTGGt                                                           <  5:76958/149‑1 (MQ=255)
                                                                  aTCCTGGTACAGGTGGAAGGCAATCACCGACCCCGCCGAGCTGGTATTGGCGTAACGGTCCAGAATCACCGGTGCCTCTTCCTCGGCCACTTCACGCCCCAACAGTTTCTTGACGATCAGCTGGTTCATGCTGAGGTTGGCCTGGTGCa                                                        <  1:110534/149‑1 (MQ=255)
                                                                     cTGGTACAGGTGGAAGGCAATCACCGACCCCGCCGAGCTGGTATTGGCGTAACGGTCCAGAATCACCGGCGCCTCTTCCTCGGCCACTTCACGCCCCAACAGTTTCTTGACGATCAGCTGGTTCATGCTGAGGTTGGCCTGGTGCAGc                                                      <  3:247411/148‑1 (MQ=255)
                                                                              gTGGAAGGCAATCACCGACCCCGCCGAGCTGGTATTGGCGTAACGGTCCAGAATCACCGGTGCCTCTTCCTCGGCCACTTCACGCCCCAACAGTTTCTTGACGATCAGCTGGTTCATGCTGAGGTTGGCCTGGTGCAGCCAGAAGCGCt                                            >  7:333615/1‑149 (MQ=255)
                                                                                     gCAATCACCGACCCCGCCGAGCTGGTATTGGCGTAACGGTCCAGAATCACCGGTGCCTCTTCCTCGGCCACTTCACGCCCCAACAGTTTCTTGACGATCAGCTGGTTCATGCTGAGGTTGGCCTGGTGCa                                                        <  7:202704/130‑1 (MQ=255)
                                                                                     gCAATCACCGACCCCGCCGAGCTGGTATTGGCGTAACGGTCCAGAATCACCGGTGCCTCTTCCTCGGCCACTTCACGCCCCAACAGTTTCTTGACGATCAGCTGGTTCATGCTGAGGTTGGCCTGGTGCa                                                        >  8:202704/1‑130 (MQ=255)
                                                                                     gCAATCACCGACCCCGCCGAGCTGGTATTGGCGTAACGGTCCAGAATCACCGGTGCCTCTTCCTCGGCCACTTCACGCCCCAACAGTTTCTTGACGATCAGCTGGTTCATGCTGAGGTTGGCCTGGTGCAGCCAGAAGCGCTTCACg                                       >  1:344453/1‑147 (MQ=255)
                                                                                     gCAATCACCGACCCCGCCGAGCTGGTATTGGCGTAACGGTCCAGAATCACCGGTGCCTCTTCCTCGGCCACTTCACGCCCCAACAGTTTCTTGACGATCAGCTGGTTCATGCTGAGGTTGGCCTGGTGCAGCCAGAAGCGCTTCACg                                       <  2:344453/147‑1 (MQ=255)
                                                                                       aaTCACCGACCCCGCCGAGCTGGTATTGGCGTAACGGTCCAGAATCACCGGTGCCTCTTCCTCGGCCACTTCACGCCCCAACAGTTTCTTGACGATCAGCTGGTTCATGCTGAGGTTGGCCTg                                                             <  5:357678/123‑1 (MQ=255)
                                                                                       aaTCACCGACCCCGCCGAGCTGGTATTGGCGTAACGGTCCAGAATCACCGGTGCCTCTTCCTCGGCCACTTCACGCCCCAACAGTTTCTTGACGATCAGCTGGTTCATGCCGAGGTTGGCCTGGTGCAGCCAGAAGCGCTTCACGTCGc                                   >  3:40097/1‑149 (MQ=255)
                                                                                       aaTCACCGACCCCGCAGAGCTGGTATTGGCGTAACGGTCCAGAATCACCGGTGCCTCTTCCTCGGCCACTTCACGCCCCAACAGTTTCTTGACGATCAGCTGGTTCATGCTGAGGTTGGCCTg                                                             >  6:357678/1‑123 (MQ=255)
                                                                                           aCCGACCCCGCCGAGCTGGTATTGGCGTAACGGTCCAGAATCACCGGTGCCTCTTCCTCGGCCACTTCACGCCCCAACAGTTTCTTGACGATCAGCTGGTTCATg                                                                           <  8:196551/105‑1 (MQ=255)
                                                                                            ccGACCCCGCCGAGCTGGTATTGGCGTAACGGTCCAGAATCACCGGTGCCTCTTCCTCGGCCACTTCACGCCCCAACAGTTTCTTGACGATCAGCTGGTTCATGCTGAGGTTGGCCTGGTGCAGCTAGAAGCGCTTCACGTCGCCTGGt                              >  7:221711/1‑149 (MQ=255)
                                                                                            ccGACCCCGCCGAGCTGGTATTGGCGTAACGGTCCAGAATCACCGGTGCCTCTTCCTCGGCCACTTCACGCCCCAACAGTTTCTTGACGATCAGCTGGTTCATGCTGAGGTTGGCCTGGTGCAGCCAGAAGCGCTTCACGTCGCCTGGt                              >  8:120689/1‑149 (MQ=255)
                                                                                                  ccgccgAGCTGGTATTGGCGTAACGGTCCAGAATCACCGGTGCCTCTTCCTCGGCCACTTCACGCCCCAACAGTTTCTTGACGATCAGCTGGTTCATGCTGAGGTTGGCCTGGTGCAGCCAGAAGCGCTTCACGTCGCCTGGTTGCAAc                        <  7:120689/149‑1 (MQ=255)
                                                                                                   cgccgAGCTGGTATTGGCGTAACGGTCCAGAATCACCGGCGCCTCTTCCTCGGCCACTTCACGCCCCAACAGTTTCTTGACGATCAGCTGGTTCATGCTGAGGTTGGCCTGGTGCAGCCAGAAGCGCTTCACGTCGCCTGGTTGCAAcc                       >  2:235355/1‑149 (MQ=255)
                                                                                                    gccgAGCTGGTATTGGCGTAACGGTCCAGAATCACCGGTGCCTCTTCCTCGGCCACTTCACGCCCCAACAGTTTCTTGACGATCAGCTGGTTCATGCTGAGGTTGGCCTGGTGCAGCCAGAAGCGCTTCACGTCGCCTGGTTGCAAccc                      <  3:324679/149‑1 (MQ=255)
                                                                                                           tGGTATTGGCGTAACGGTCCAGAATCACCGGTGCCTCTTCCTCGGCCACTTCACGCCCCAACAGTTTCTTGACGATCAGCTGGTTCATGCTGAGGTTGGCCTGGTGCAGCCAGAAGCGCTTCACGTCGCCTGGTTGCAACCCGTTCTcc               >  5:236501/1‑149 (MQ=255)
                                                                                                              tATTGGCGTAACGGTCAAGTATAACCTGTGCCTCTTCCTCGGCCTCTTCACGCGCCAAGATTTTCTTGACGATCAGCTGGTGGATGCGGAGGTTGGCGTGGTGCAGGCAGAAGCTCTTCAGGTCGCCTGGTTGCAACACTTTCTCCTgg            <  4:40097/149‑2 (MQ=255)
                                                                                                                        aCGGTCCAGAATCACCGGTGCCTCTTCCTCGGCCACTTCACGCCCCAACAGTTTCTTGACGATCAGCTGGTTCATGCTGAGGTTGGCCTGGTGCAGCCAGAAGCGCTTCACGTCGCCTGGTTGCAACCCGTTCTCCTGCAGGTGCTCAc  <  8:333615/149‑1 (MQ=255)
                                                                                                                        aCGGTCCAGAATCACCGGCGCCTCTTCCTCGGCCACTTCACGCCCCAACAGTTTCTTGACGATCAGCTGGTTCATGCTGAGGTTGGCCTGGTGCAGCCAGAAGCGCTTCACGTTGCCTGGTTGCAACCCGTTCTCCTGCAGGTGCTCAc  <  1:235355/149‑1 (MQ=255)
                                                                                                                                          tGCCTCTTCCTCGGCCACTTCACGCCCCAACAGTTTCTTGACGATCAGCTGGTTCATGCTGAGGTTGGCCTGGTGCAGCCAGAAGCGCTTCACGTc                                     <  3:175601/96‑1 (MQ=255)
                                                                                                                                          tGCCTCTTCCTCGGCCACTTCACGCCCCAACAGTTTCTTGACGATCAGCTGGTTCATGCTGAGGTTGGCCTGGTGCAGCCAGAAGCGCTTCACGTc                                     >  4:175601/1‑96 (MQ=255)
                                                                                                                                          |                                                                                                                                  
CACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAGGTCATCCTGGTACAGGTGGAAGGCAATCACCGACCCCGCCGAGCTGGTATTGGCGTAACGGTCCAGAATCACCGGTGCCTCTTCCTCGGCCACTTCACGCCCCAACAGTTTCTTGACGATCAGCTGGTTCATGCTGAGGTTGGCCTGGTGCAGCCAGAAGCGCTTCACGTCGCCTGGTTGCAACCCGTTCTCCTGCAGGTGCTCAC  >  NC_002947/5162696‑5162964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑