| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,177,680 | T→A | 10.8% | D102V (GAT→GTT) | PP_4553 ← | ECF family RNA polymerase sigma‑70 factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,177,680 | 0 | T | A | 10.8% | 102.7 / 3.6 | 37 | D102V (GAT→GTT) | PP_4553 | ECF family RNA polymerase sigma‑70 factor |
| Reads supporting (aligned to +/- strand): ref base T (17/16); new base A (2/2); total (19/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
GGCGATGGTGGCCGGGCTTTGCAGAAAGGCGCGGGCGATCTGCTCGGTGGTCAGGTCGCAGACTTCGCGAAGCGTCAACGGCACCTGAGCGTCGGCCGACAGGGCCGGATGGCAACAGGTGAAGATCAGCCGCAGGCGGTCATCCGCCAGCAGCTCTTCTTCACTGGGGTCCTGCGCCTGACCCTCCAGCAGCATGCTCAAGTCAGCCTGGGAGCGGTCGAAGCGGACGCGCCGGCGCAGCGCGTCAATGGCCTTGAAGCGCCCGACAGAAACCAGCCAC > NC_002947/5177538‑5177817 | ggCGATGGTGGCCGGGCTTTGCAGAAAGGCGCGGGCGATCTGCTCGGTGGTCAGGTCGCAGACTTCGCGAAGCGTCAACGGCACCTAAGCGTCGGCCGACAGGGCCGGATGGCAACAGGTGAAGATCAGCCGCAGGCGGTCATCCGcc < 6:264656/148‑1 (MQ=255) gCGATGGTGGCCGGGCTTTGCAGAAAGGCGCGGGCGATCTGCTCGGTGGTCAGGTCGCAGACTTCGCGAAGCGTCAACGGCACCTGAGCGTCGGCCGACAGGGCCGGATGGCAACAGGTGAAGATCAGCCGCAGGCGGTCATCCGCcag < 7:179030/149‑1 (MQ=255) gATGGTGGCCGGGCTTTGCAGAAAGGCGCGGGCGATCTGCTCGGTGGTCAGGTCGCAGACTTCGCGAAGCGTCAACGGTACCTGAGCGTCGGCCGACAGGGCCGGATGGCAACAGGTGAAGATCAGCCGCAGGCGGTCATCCGCcagca < 3:312985/149‑1 (MQ=255) tGCAGAAAGGCGCGGGCGATCTGCTCGGTGGTCAGGTCGCAGACTTCGCGAAGCGTCAACGGCACCTGAGCGTCGGCCGACAGGGCCGGATGGCAACAGGTGAAGATCAGCCGCAGGCGGTCATCCGCCAGCAGCTCTTCTTCACTg < 8:185852/147‑1 (MQ=255) cAGAAAGGCGCGGGCGATCTGCTCGGTGGTCAGGTCGCAGACTTCGCGAAGCGTCAACGGCACCTGAGCGTCGGCCGACAGGGCCGGATGGCAACAGGTGAAGAAGAGCTGCTGGCGGATGACCGCCTGCGGCTGATCTTCAcctgttg < 4:326264/149‑7 (MQ=21) aGAAAGGCGCGGGCGATCTGCTCGGTGGTCAGGTCGCAGACTTCGCGAAGCGTCAACGGCACCTGAGCGTCGGCCGACAGGGCCGGATGGCAACAGGTGAAGATCAGCCGCAGGCGGTCAACCGCCAGCAGCTCTTCTTCACTGGGGTc > 2:12412/1‑149 (MQ=255) tCAGGTCGCAGACTTCGCGAAGCGTCAACGGCACCTGAGCGTCGGCCGACAGGGCCGGATGGCAACAGGTGAAGATCAGCCGCAGGCGGTCATCCGCCAGCAGCTCTTCTTCACTGGGGTCCTGCGCCTGACCCTCCAGCAGCATGCt > 6:193914/1‑148 (MQ=255) gTCGCAGACTTCGCGAAGCGTCAACGGCACCTGAGCGTCGGCCGACAGGGCCGGATGGCAACAGGTGAAGATCAGCCGCAGGCGGTCATCCGCCAGCAGCTCTTCTTCACTGGGGTCCTGCGCCTGACCCTCCAGCAGCATGCTCAAGt > 4:155104/1‑149 (MQ=255) cAGACTTCGCGAAGCGTCAACGGCACCTGAGCGTCGGCCGACAGGGCCGGATGGCAACAGGTGAAGATCAGCCGCAGGCGGTCATCCGCCAGCAGCTCTTCTTCACTGGGGTCCTGCGCCTGACCCTCCAGCAGCATGCTCAAGTCAGc < 3:167451/149‑1 (MQ=255) aCTTCGCGAAGCGTCAACGGCACCTGAGCGTCGGCCGACAGGGCCGGATGGCAACAGGTGAAGATCAGCCGCAGGCGGTCATCCGCCAGCAGCTCTTCTTCACTGGGGTCCTGCGCCTGACCCTCCAGCAGCATGCTCAAGTCAGCCTg > 6:199419/1‑149 (MQ=255) cTTCGCGAAGCGTCAACGGCACCTGAGCGTCGGCCGACAGGGCCGGATGGCAACAGGTGAAGATCAGCCGCAGGCGGTCATCCGCCAGCAGCTCTTCTTCACTGGGGTCCTGCGCCTGACCCTCCAGCAGCATGCTCAAGTCAGCCCgg < 8:214832/149‑1 (MQ=255) ttCGCGAAGCGTCAATGGCACCTGAGCGTCGGCCGACAGGGCCGGATGGCAACAGGTGAAGATCAGCCGCAGGCGGTCATCCGCCAGCAGCTCTTCTTCACTGGGGTCCTGCGCCTGACCCTCCAGCAGCATGCTCAAGTCAGCCTgg > 2:223419/1‑148 (MQ=255) ttCGCGAAGCGTCAACGGCACCTGAGCGTCGGCCGACAGGGCCGGATGGCAACAGGTGAAGATCAGCCGCAGGCGGTCATCCGCCAGCAGCTCTTCTTCACTGGGGTCCTGCGcc < 3:73435/115‑1 (MQ=255) ttCGCGAAGCGTCAACGGCACCTGAGCGTCGGCCGACAGGGCCGGATGGCAACAGGTGAAGATCAGCCGCAGGCGGTCATCCGCCAGCAGCTCTTCTTCACTGGGGTCCTGCGcc > 4:73435/1‑115 (MQ=255) gAAGCGTCAACGGCACCTGAGCGTCGGCCGACAGGGCCGGATGGCAACAGGTGAAGATCAGCCGCAGGCGGTCATCCGCCAGCAGCTCTTCTTCACTGGGGTCCTGCGCCTGACCCTCCAgcagca > 2:90524/1‑126 (MQ=255) gAAGCGTCAACGGCACCTGAGCGTCGGCCGACAGGGCCGGATGGCAACAGGTGAAGATCAGCCGCAGGCGGTCATCCGCCAGCAGCTCTTCTTCACTGGGGTCCTGCGCCTGACCCTCCAgcagca < 1:90524/126‑1 (MQ=255) tCAACGGCACCTGAGCGTCGGCCGACAGGGCCGGATGGCAACAGGTGAAGATCAGCCGCAGGCGGTCATCCGCCAGCAGCTCTTCTTCACTGGGGTCCTGCGCCTGACCCTCCAGCAGCATGCTCAAGTCAGCCTGGGAGCGGTCGAAg > 2:165013/1‑149 (MQ=255) aaCGGCACCTGAGCGTCGGCCGACAGGGCCGGATGGCAACAGGTGAAGATCAGCCGCAGGCGGTCAACCGCCAGCAGCTCTTCTTCACTGGGGTCCTGCGCCTGACCCTCCAGCAGCATGCTCAAGTCAGCCTGGGAGCGGTCGAAGc < 1:12412/148‑1 (MQ=255) gAGCGTCGGCCGACAGGGCCGGATGGCAACAGGTGAAGATCAGCCGCAGGCGGTCATCCGCCAGCAGCTCTTCTTCACTGGGGTCCTGCGCCTGACCCTCCAGCAGCATGCTCAAGTCAGCCTGGGAGCGGTCGAAGCGGa < 6:263269/141‑1 (MQ=255) gAGCGTCGGCCGACAGGGCCGGATGGCAACAGGTGAAGATCAGCCGCAGGCGGTCATCCGCCAGCAGCTCTTCTTCACTGGGGTCCTGCGCCTGACCCTCCAGCAGCATGCTCAAGTCAGCCTGGGAGCGGTCGAAGCGGa > 5:263269/1‑141 (MQ=255) gTCGGCCGACAGGGCCGGATGGCAACAGGTGAAGATCAGCCGCAGGCGGTCATCCGCCAGCAGCTCTTCTTCACTGGGGTCCTGCGCCTGACCCTCCAGCAGCATGCTCAAGTCAGCCTGGGAGCGGTCGAAGCGGACGCGCCGgcg > 7:175091/1‑147 (MQ=255) tCGGCCGACAGGGCCGGATGGCAACAGGTGAAGATCAGCCGCAGGCGGTCATCCGCCAGCAGCTCTTCTTCACTg < 2:290347/75‑1 (MQ=255) tCGGCCGACAGGGCCGGATGGCAACAGGTGAAGATCAGCCGCAGGCGGTCATCCGCCAGCAGCTCTTCTTCACTg > 1:290347/1‑75 (MQ=255) aGGGCCGGATGGCAACAGGTGAAGATCAGCCGCAGGCGGTCATCCGCCAGCAGCTCTTCTTCACTGGGGTCCTGCGCCTGACCCTCCAGCAGCATGCTCAAGTCAGCCTGGGAGCGGTCGAAGCGGACGCGCCGGCGCAGCGCGTCAAt > 4:344990/1‑149 (MQ=255) aGGGCCGGATGGCAACAGGTGAAGATCAGCCGCAGGCGGTCATCCGCCAGCAGCTCTTCTTCACTGGGGTCCTGCGCCTGACCCTCCAGCAGCATGCTCAAGTCAGCCTGGGAGCGGTCGAAGCGGACGCGCCGGCGCAGCGCGTCAAt < 1:165013/149‑1 (MQ=255) ccGGATGGCAACAGGTGAAGATCAGCCGCAGGCGGTCATCCGCCAGCAGCTCTTCTTCACTGGGGTCCTGCGCCTGACCCTCCAGCAGCATGCTCAAGTCAGCCTGGGAGCGGTCGAAGCGGACGCGCCGGCGCAGCGCGTCAATGGcc < 6:183190/149‑1 (MQ=255) gATGGCAACAGGTGAAGATCAGCCGCAGGCGGTCATCCGCCAGCAGCTCTTCTTCACTGGGGTCCTGCGCCTGACCCTCCAGCAGCATGCTCAAGTCAGCCTGGGAGCGGTCGAAGCGGACGCGCCGGCGCAGCGCGTCAATGGCCtt > 1:203860/1‑148 (MQ=255) aaCAGGTGAAGATCAGCCGCAGGCGGTCATCCGCCAGCAGCTCTTCTTCACTGGGGTCCTg > 5:365944/1‑61 (MQ=255) aaCAGGTGAAGATCAGCCGCAGGCGGTCATCCGCCAGCAGCTCTTCTTCACTGGGGTCCTg < 6:365944/61‑1 (MQ=255) gTGAAGAAGAGCTGCTGGCGGATGACCGCCTGCGGCTGATCTTCACTGGGGTCCTGCGCCTGACCCTCCAGCAGCATGCTCAAGTCAGCCTGGGAGCGGTCGAAGCGGACGCGCCGGCGCAGCGCGTCAATGGCCTTGAAGCGCCCGa > 4:119076/1‑148 (MQ=25) tCAGCCGCAGGCGGTCATCCGCCAGCAGCTCTTCTTCACTGGGGTCCTGCGCCTGACCCTCCAGCAGCATGCTCAAGTCAGCCTGGGAGCGGTCGAAGCGGACGCGCCGGCGCAGCGCGTCAATGGCCTTGAAGCGCCCGACAGAAAc > 3:85186/1‑148 (MQ=255) ccGCAGGCGGTCATCCGCCAGCAGCTCTTCTTCACTGGGGTCCTGCGCCTGACCCTCCAGCAGCATGCTCAAGTCAGCCTGGGAGCGGTCGAAGCGGACGCGCCGGCGCAGCGCGTCAATGGCCTTGaa > 6:295488/1‑129 (MQ=255) ccGCAGGCGGTCATCCGCCAGCAGCTCTTCTTCACTGGGGTCCTGCGCCTGACCCTCCAGCAGCATGCTCAAGTCAGCCTGGGAGCGGTCGAAGCGGACGCGCCGGCGCAGCGCGTCAATGGCCTTGAAGCGCCCGACAGAAACCAGcc < 3:344990/149‑1 (MQ=255) gCAGGCGGTCATCCGCCAGCAGCTCTTCTTCACTGGGGTCCTGCGCCTGACCCTCCAGCAGCATGCTCAAGTCAGCCTGGGAGCGGTCGAAGCGGACGCGCCGGCGCAGCGCGTCAATGGCCTTGAAGCGCCCGACAGAAACCAGCCAc < 2:203860/149‑1 (MQ=255) gCAGGCGGTCATCCGCCAGCAGCTCTTCTTCACTGGGGTCCTGCGCCTGACCCTCCAGCAGCATGCTCAAGTCAGCCTGGGAGCGGTCGAAGCGGACGCGCCGGCGCAGCGCGTCAATGGCCTTGAAGCGCCCGACAGAAACCAGCCAc > 7:17483/1‑149 (MQ=255) aTCCGCCAGCAGCTCTTCTTCACTGGGGTCCTGCGCCTGACCCTCCAGCAGCATGCTCAAGTCAGCCTGGGAGCGGTCGAAGCGGACGCGCCGGCGCAGCGCGTCAATGGCCTTGAAGCGccc > 5:278569/1‑123 (MQ=255) aTCCGCCAGCAGCTCTTCTTCACTGGGGTCCTGCGCCTGACCCTCCAGCAGCATGCTCAAGTCAGCCTGGGAGCGGTCGAAGCGGACGCGCCGGCGCAGCGCGTCAATGGCCTTGAAGCGccc < 6:278569/123‑1 (MQ=255) | GGCGATGGTGGCCGGGCTTTGCAGAAAGGCGCGGGCGATCTGCTCGGTGGTCAGGTCGCAGACTTCGCGAAGCGTCAACGGCACCTGAGCGTCGGCCGACAGGGCCGGATGGCAACAGGTGAAGATCAGCCGCAGGCGGTCATCCGCCAGCAGCTCTTCTTCACTGGGGTCCTGCGCCTGACCCTCCAGCAGCATGCTCAAGTCAGCCTGGGAGCGGTCGAAGCGGACGCGCCGGCGCAGCGCGTCAATGGCCTTGAAGCGCCCGACAGAAACCAGCCAC > NC_002947/5177538‑5177817 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |