Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,182,059 G→T 7.7% intergenic (‑117/+37) PP_4560 ← / ← csbD ribonuclease BN/stress response protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,182,0590GT 7.7% 197.1 / 6.4 65intergenic (‑117/+37)PP_4560/csbDribonuclease BN/stress response protein
Reads supporting (aligned to +/- strand):  ref base G (32/28);  new base T (2/3);  total (34/31)
Fisher's exact test for biased strand distribution p-value = 6.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.34e-01

GGCAAGCCGCGCAGGTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCACCTT  >  NC_002947/5181914‑5182203
                                                                                                                                                 |                                                                                                                                                
ggCAAGCCGCGCAGGTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCgg                                                                                                                                               <  8:118390/149‑1 (MQ=255)
     gCCGCGCAGGTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCa                                                                                                                                          >  2:211593/1‑149 (MQ=255)
          gcAGGTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCga                                                                                                                                     <  4:13317/149‑1 (MQ=255)
             ggTCGGGGAAAATCATAGCGTCTCCTTTCGGCGCTTGATCGTTACACAGGCAAGTCTAATAGAGCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTTAAGAATTTGCTTACGAATTAGTGCCGGGCTGTTCTCGGGTGAACCCGcgaa                                                                                                                                  <  2:125821/149‑1 (MQ=255)
             ggTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGagaa                                                                                                                                  <  6:317724/149‑1 (MQ=255)
               tCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCTCGGGTGAACCCGcgaaga                                                                                                                                <  5:12913/149‑1 (MQ=255)
                 ggggAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCgg                                                                                                                                               <  4:297284/132‑1 (MQ=255)
                 ggggAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCgg                                                                                                                                               >  3:297284/1‑132 (MQ=255)
                   ggAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAagag                                                                                                                               >  6:136668/1‑146 (MQ=255)
                   ggAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAagag                                                                                                                               <  5:136668/146‑1 (MQ=255)
                   ggAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGcc                                                                                                                            >  5:80741/1‑149 (MQ=255)
                    gAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCg                                                                                                                           >  4:68798/1‑149 (MQ=255)
                    gAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCg                                                                                                                           <  4:141659/149‑1 (MQ=255)
                    gAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACAAATAAGTGCCGGCCTCTTCTCGCGTGAACCCGCGAAGAGGCCg                                                                                                                           <  8:48081/149‑1 (MQ=39)
                         tCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGagaaga                                                                                                                                >  3:232854/1‑139 (MQ=255)
                         tCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGagaaga                                                                                                                                <  4:232854/139‑1 (MQ=255)
                           aTAGCGTCTCATTTCGCCGCTTGATCTTAACACAGGCAAGTCTAATAGACCGTGCCCGAGCGGTGCTGCTCCCGGCCGTGGTTTAATAATTTTCTTACTAATAAGTGCCGGCCTCTTCGCGGTTTCACCCGATAATTGGCCGGCACCGc                                                                                                                    <  6:317284/149‑1 (MQ=255)
                                gTCTCCTTTCGCCGCTTGATCGTTACACAGCCATTTCTAATAGACCGTGCCCGTGCTGTGCTGCTCCCGGCCGTGGTTTAATAATTTGCTTACTAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGATATGAGGCCGGCACCGCCtcaa                                                                                                               <  6:80741/149‑1 (MQ=255)
                                 tctcCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGACCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCtcaag                                                                                                              >  6:126575/1‑149 (MQ=255)
                                         cgccgcTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTTAAGAATTTGCTTACCAATACGTGCCGGCCTCTTCGCGGGTGCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGc                                                                                                       <  7:205933/148‑1 (MQ=255)
                                                 gATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGATAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCAc                                                                                               <  4:216908/148‑1 (MQ=255)
                                                 gATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCAcc                                                                                              >  2:63060/1‑149 (MQ=255)
                                                  aTCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAgg                                                                                                                              >  5:208205/1‑116 (MQ=255)
                                                  aTCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAgg                                                                                                                              <  6:208205/116‑1 (MQ=255)
                                                    cGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGATTCTTAACCGCa                                                                                           >  3:219166/1‑149 (MQ=255)
                                                    cGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCAACGCCTCAAGTCAAGGCTTCTTCAACGCa                                                                                           >  3:223578/1‑149 (MQ=255)
                                                     gTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCa                                                                                           <  3:253973/148‑1 (MQ=255)
                                                         cacaGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCtt                                                                                      <  5:133902/149‑1 (MQ=255)
                                                          acaGCCAAGTCTAATACACCTTCCCCGAGCTGTGCTGCTCCCGGCCGTGGTTCAACAATTTGCTTACCAATAAGTGCCGCCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTc                                                                                     <  4:82996/149‑1 (MQ=255)
                                                                  gTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTc                                                                              >  6:154467/1‑148 (MQ=255)
                                                                  gTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTc                                                                              <  5:154467/148‑1 (MQ=255)
                                                                     tAATAGACCGTGACCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCtt                                                                          <  5:126575/149‑1 (MQ=255)
                                                                           aCCGTGCCCGAGCTGTGCTGCTCCCGGCTGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACctg                                                                    >  7:230027/1‑149 (MQ=255)
                                                                             cGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGc                                                                                                       >  3:157858/1‑112 (MQ=255)
                                                                               tGCCTGAGTTGTGCTGCTCCCGGCGGTGGTGTATTATTTTTATTACTATTAAGTGCCGGCGTTTTGGGGGGTTCACCCTATAATTGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCtgctgc                                                                <  4:223578/149‑1 (MQ=255)
                                                                                  ccGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGcgcc                                                             >  2:266129/1‑149 (MQ=255)
                                                                                    gAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGACTCTTAGCGGGTTCACACGAGAAGAGTCCGGCACCGCCtcaagtcaag                                                                                                         >  6:321409/1‑103 (MQ=255)
                                                                                    gAGCTGTGCTGCGCCCGGCCGTGGTTGAATAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGTCCGGCACCGCCtcaagtcaag                                                                                                         <  5:321409/103‑1 (MQ=255)
                                                                                            ctgctCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGa                                                   <  1:63060/149‑1 (MQ=255)
                                                                                                tCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTc                                               >  8:251938/1‑149 (MQ=255)
                                                                                                        gTGGTTGAAGAATTTGCTTACGTATAAGTGCCGGACTATTCGCGGGTTCACCAGAGAAGAGGCCGGCACCGCCACAAGTCCAGGCTTATTCACCGCAGCGGTCACATAGCGCTTGACCTGCTGCGCCGCGCGTTTGAGCTCCTgcgagt                                       >  7:55469/1‑146 (MQ=255)
                                                                                                              gaagaaTTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGt                                                                                                                                             >  5:8266/1‑41 (MQ=255)
                                                                                                              gaagaaTTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGt                                                                                                                                             <  6:8266/41‑1 (MQ=255)
                                                                                                              gaagaaTTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCgccttgcctt                                 >  4:114558/1‑149 (MQ=255)
                                                                                                              gaagaaTTTGCTTACGAATAAGTGCCGGCCTCTTCGAGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCATCACATTTCCCTTGACATACTGCGCCTCGCCTTTGAGCTACTGCgacttgcat                                  >  4:104333/1‑146 (MQ=255)
                                                                                                               aagaaTTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCAGCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCAcc                                                                                              <  2:43355/87‑1 (MQ=255)
                                                                                                               aagaaTTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCAGCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCAcc                                                                                              >  1:43355/1‑87 (MQ=255)
                                                                                                               aagaaTTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTc                                >  4:239754/1‑149 (MQ=255)
                                                                                                                atattttGGTTACCAATACGTCCCGGCCGCTTCGCGGGTGCCCCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCg                               <  8:230027/145‑1 (MQ=255)
                                                                                                                 gaaTTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCgg                              <  6:183846/149‑1 (MQ=255)
                                                                                                                         ttACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTATTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGc                      >  6:245563/1‑149 (MQ=255)
                                                                                                                          tACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCAACCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCt                     >  3:155920/1‑149 (MQ=255)
                                                                                                                           aCGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCtt                    >  5:196116/1‑149 (MQ=255)
                                                                                                                            ctaataatTGCCGGCCTCTTCGCGGTTTCACCCGATAAGAGGCCGGCACCGCCTCGAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCttt                   <  3:68798/147‑1 (MQ=255)
                                                                                                                             gAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCtttt                  >  1:191706/1‑149 (MQ=255)
                                                                                                                               aTAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCg                <  1:211593/149‑1 (MQ=255)
                                                                                                                               aTAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCg                >  7:91476/1‑149 (MQ=255)
                                                                                                                                   gagcctgcctCTACGCTGGTACACCAGAGAATAGGCCGGCACCGCCTCCAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGCCCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGt            <  4:262545/147‑1 (MQ=255)
                                                                                                                                   gTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGt            >  2:39721/1‑149 (MQ=255)
                                                                                                                                   gTGCCGGCAGATTCGCGGTTGCACACTCGAAGAGGCCGGCACCGGCTCCAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGt            <  4:267389/149‑1 (MQ=255)
                                                                                                                                      ccGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCgg         >  3:211058/1‑149 (MQ=255)
                                                                                                                                       cGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGt        >  1:100159/1‑149 (MQ=255)
                                                                                                                                        ggCCTCTTCTCGGGTGAACCCGCGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGt        >  4:69454/1‑148 (MQ=255)
                                                                                                                                         gCCTCTTCTCGGGTGAACCCGCGAAGAGGCCGGCACCGCCTCAAGTCACGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCa      >  1:204868/1‑149 (MQ=39)
                                                                                                                                         gCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCa      >  5:339249/1‑149 (MQ=255)
                                                                                                                                                cgcgGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCACCtt  <  3:104333/146‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GGCAAGCCGCGCAGGTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCACCTT  >  NC_002947/5181914‑5182203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑