Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,182,065 T→G 16.8% intergenic (‑123/+31) PP_4560 ← / ← csbD ribonuclease BN/stress response protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,182,0650TG16.8% 135.4 / 18.9 64intergenic (‑123/+31)PP_4560/csbDribonuclease BN/stress response protein
Reads supporting (aligned to +/- strand):  ref base T (30/22);  new base G (3/8);  total (33/31)
Fisher's exact test for biased strand distribution p-value = 9.79e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCGCGCAGGTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCACCTT  >  NC_002947/5181919‑5182203
                                                                                                                                                  |                                                                                                                                          
gCCGCGCAGGTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCa                                                                                                                                          >  2:211593/1‑149 (MQ=255)
     gcAGGTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCga                                                                                                                                     <  4:13317/149‑1 (MQ=255)
        ggTCGGGGAAAATCATAGCGTCTCCTTTCGGCGCTTGATCGTTACACAGGCAAGTCTAATAGAGCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTTAAGAATTTGCTTACGAATTAGTGCCGGGCTGTTCTCGGGTGAACCCGcgaa                                                                                                                                  <  2:125821/149‑1 (MQ=255)
        ggTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGagaa                                                                                                                                  <  6:317724/149‑1 (MQ=255)
          tCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCTCGGGTGAACCCGcgaaga                                                                                                                                <  5:12913/149‑1 (MQ=255)
              ggAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAagag                                                                                                                               <  5:136668/146‑1 (MQ=255)
              ggAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAagag                                                                                                                               >  6:136668/1‑146 (MQ=255)
              ggAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGcc                                                                                                                            >  5:80741/1‑149 (MQ=255)
               gAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCg                                                                                                                           >  4:68798/1‑149 (MQ=255)
               gAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCg                                                                                                                           <  4:141659/149‑1 (MQ=255)
               gAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACAAATAAGTGCCGGCCTCTTCTCGCGTGAACCCGCGAAGAGGCCg                                                                                                                           <  8:48081/149‑1 (MQ=39)
                    tCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGagaaga                                                                                                                                <  4:232854/139‑1 (MQ=255)
                    tCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGagaaga                                                                                                                                >  3:232854/1‑139 (MQ=255)
                      aTAGCGTCTCATTTCGCCGCTTGATCTTAACACAGGCAAGTCTAATAGACCGTGCCCGAGCGGTGCTGCTCCCGGCCGTGGTTTAATAATTTTCTTACTAATAAGTGCCGGCCTCTTCGCGGTTTCACCCGATAATTGGCCGGCACCGc                                                                                                                    <  6:317284/149‑1 (MQ=255)
                           gTCTCCTTTCGCCGCTTGATCGTTACACAGCCATTTCTAATAGACCGTGCCCGTGCTGTGCTGCTCCCGGCCGTGGTTTAATAATTTGCTTACTAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGATATGAGGCCGGCACCGCCtcaa                                                                                                               <  6:80741/149‑1 (MQ=255)
                            tctcCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGACCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCtcaag                                                                                                              >  6:126575/1‑149 (MQ=255)
                                    cgccgcTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTTAAGAATTTGCTTACCAATACGTGCCGGCCTCTTCGCGGGTGCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGc                                                                                                       <  7:205933/148‑1 (MQ=255)
                                            gATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGATAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCAc                                                                                               <  4:216908/148‑1 (MQ=255)
                                            gATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCAcc                                                                                              >  2:63060/1‑149 (MQ=255)
                                             aTCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAgg                                                                                                                              <  6:208205/116‑1 (MQ=255)
                                             aTCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAgg                                                                                                                              >  5:208205/1‑116 (MQ=255)
                                               cGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGATTCTTAACCGCa                                                                                           >  3:219166/1‑149 (MQ=255)
                                               cGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCAACGCCTCAAGTCAAGGCTTCTTCAACGCa                                                                                           >  3:223578/1‑149 (MQ=255)
                                                gTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCa                                                                                           <  3:253973/148‑1 (MQ=255)
                                                    cacaGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCtt                                                                                      <  5:133902/149‑1 (MQ=255)
                                                     acaGCCAAGTCTAATACACCTTCCCCGAGCTGTGCTGCTCCCGGCCGTGGTTCAACAATTTGCTTACCAATAAGTGCCGCCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTc                                                                                     <  4:82996/149‑1 (MQ=255)
                                                             gTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTc                                                                              <  5:154467/148‑1 (MQ=255)
                                                             gTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTc                                                                              >  6:154467/1‑148 (MQ=255)
                                                                tAATAGACCGTGACCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCtt                                                                          <  5:126575/149‑1 (MQ=255)
                                                                      aCCGTGCCCGAGCTGTGCTGCTCCCGGCTGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACctg                                                                    >  7:230027/1‑149 (MQ=255)
                                                                        cGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGc                                                                                                       >  3:157858/1‑112 (MQ=255)
                                                                          tGCCTGAGTTGTGCTGCTCCCGGCGGTGGTGTATTATTTTTATTACTATTAAGTGCCGGCGTTTTGGGGGGTTCACCCTATAATTGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCtgctgc                                                                <  4:223578/149‑1 (MQ=255)
                                                                             ccGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGcgcc                                                             >  2:266129/1‑149 (MQ=255)
                                                                               gAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGACTCTTAGCGGGTTCACACGAGAAGAGTCCGGCACCGCCtcaagtcaag                                                                                                         >  6:321409/1‑103 (MQ=255)
                                                                               gAGCTGTGCTGCGCCCGGCCGTGGTTGAATAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGTCCGGCACCGCCtcaagtcaag                                                                                                         <  5:321409/103‑1 (MQ=255)
                                                                                       ctgctCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGa                                                   <  1:63060/149‑1 (MQ=255)
                                                                                           tCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTc                                               >  8:251938/1‑149 (MQ=255)
                                                                                                   gTGGTTGAAGAATTTGCTTACGTATAAGTGCCGGACTATTCGCGGGTTCACCAGAGAAGAGGCCGGCACCGCCACAAGTCCAGGCTTATTCACCGCAGCGGTCACATAGCGCTTGACCTGCTGCGCCGCGCGTTTGAGCTCCTgcgagt                                       >  7:55469/1‑146 (MQ=255)
                                                                                                         gaagaaTTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCgccttgcctt                                 >  4:114558/1‑149 (MQ=255)
                                                                                                         gaagaaTTTGCTTACGAATAAGTGCCGGCCTCTTCGAGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCATCACATTTCCCTTGACATACTGCGCCTCGCCTTTGAGCTACTGCgacttgcat                                  >  4:104333/1‑146 (MQ=255)
                                                                                                          aagaaTTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCAGCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCAcc                                                                                              <  2:43355/87‑1 (MQ=255)
                                                                                                          aagaaTTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCAGCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCAcc                                                                                              >  1:43355/1‑87 (MQ=255)
                                                                                                          aagaaTTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTc                                >  4:239754/1‑149 (MQ=255)
                                                                                                           atattttGGTTACCAATACGTCCCGGCCGCTTCGCGGGTGCCCCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCg                               <  8:230027/145‑1 (MQ=255)
                                                                                                            gaaTTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCgg                              <  6:183846/149‑1 (MQ=255)
                                                                                                                    ttACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTATTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGc                      >  6:245563/1‑149 (MQ=255)
                                                                                                                     tACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCAACCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCt                     >  3:155920/1‑149 (MQ=255)
                                                                                                                      aCGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCtt                    >  5:196116/1‑149 (MQ=255)
                                                                                                                       ctaataatTGCCGGCCTCTTCGCGGTTTCACCCGATAAGAGGCCGGCACCGCCTCGAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCttt                   <  3:68798/147‑1 (MQ=255)
                                                                                                                        gAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCtttt                  >  1:191706/1‑149 (MQ=255)
                                                                                                                          aTAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCg                >  7:91476/1‑149 (MQ=255)
                                                                                                                          aTAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCg                <  1:211593/149‑1 (MQ=255)
                                                                                                                              gagcctgcctCTACGCTGGTACACCAGAGAATAGGCCGGCACCGCCTCCAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGCCCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGt            <  4:262545/147‑1 (MQ=255)
                                                                                                                              gTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGt            >  2:39721/1‑149 (MQ=255)
                                                                                                                              gTGCCGGCAGATTCGCGGTTGCACACTCGAAGAGGCCGGCACCGGCTCCAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGt            <  4:267389/149‑1 (MQ=255)
                                                                                                                                 ccGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCgg         >  3:211058/1‑149 (MQ=255)
                                                                                                                                  cGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGt        >  1:100159/1‑149 (MQ=255)
                                                                                                                                   ggCCTCTTCTCGGGTGAACCCGCGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGt        >  4:69454/1‑148 (MQ=255)
                                                                                                                                    gCCTCTTCTCGGGTGAACCCGCGAAGAGGCCGGCACCGCCTCAAGTCACGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCa      >  1:204868/1‑149 (MQ=39)
                                                                                                                                    gCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCa      >  5:339249/1‑149 (MQ=255)
                                                                                                                                           ctcgGGTGAACCCGCGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTgc                                           >  7:90218/3‑105 (MQ=39)
                                                                                                                                           ctcgGGTGAACCCGCGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTgc                                           <  8:90218/103‑1 (MQ=39)
                                                                                                                                           ctcgGGTGAACCCGCGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGc                             <  6:72550/117‑1 (MQ=255)
                                                                                                                                           cgcgGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCACCtt  <  3:104333/146‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                          
GCCGCGCAGGTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCACCTT  >  NC_002947/5181919‑5182203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑