Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,182,072 A→C 10.8% intergenic (‑130/+24) PP_4560 ← / ← csbD ribonuclease BN/stress response protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,182,0720AC10.8% 153.8 / 9.0 62intergenic (‑130/+24)PP_4560/csbDribonuclease BN/stress response protein
Reads supporting (aligned to +/- strand):  ref base A (32/23);  new base C (3/4);  total (35/27)
Fisher's exact test for biased strand distribution p-value = 6.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

GCAGGTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCACCTTGCCAACACCCTGTTTC  >  NC_002947/5181924‑5182219
                                                                                                                                                    |                                                                                                                                                   
gcAGGTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCga                                                                                                                                                     <  4:13317/149‑1 (MQ=255)
   ggTCGGGGAAAATCATAGCGTCTCCTTTCGGCGCTTGATCGTTACACAGGCAAGTCTAATAGAGCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTTAAGAATTTGCTTACGAATTAGTGCCGGGCTGTTCTCGGGTGAACCCGcgaa                                                                                                                                                  <  2:125821/149‑1 (MQ=255)
   ggTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGagaa                                                                                                                                                  <  6:317724/149‑1 (MQ=255)
     tCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCTCGGGTGAACCCGcgaaga                                                                                                                                                <  5:12913/149‑1 (MQ=255)
         ggAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAagag                                                                                                                                               >  6:136668/1‑146 (MQ=255)
         ggAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAagag                                                                                                                                               <  5:136668/146‑1 (MQ=255)
         ggAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGcc                                                                                                                                            >  5:80741/1‑149 (MQ=255)
          gAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCg                                                                                                                                           >  4:68798/1‑149 (MQ=255)
          gAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCg                                                                                                                                           <  4:141659/149‑1 (MQ=255)
          gAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACAAATAAGTGCCGGCCTCTTCTCGCGTGAACCCGCGAAGAGGCCg                                                                                                                                           <  8:48081/149‑1 (MQ=39)
               tCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGagaaga                                                                                                                                                >  3:232854/1‑139 (MQ=255)
               tCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGagaaga                                                                                                                                                <  4:232854/139‑1 (MQ=255)
                 aTAGCGTCTCATTTCGCCGCTTGATCTTAACACAGGCAAGTCTAATAGACCGTGCCCGAGCGGTGCTGCTCCCGGCCGTGGTTTAATAATTTTCTTACTAATAAGTGCCGGCCTCTTCGCGGTTTCACCCGATAATTGGCCGGCACCGc                                                                                                                                    <  6:317284/149‑1 (MQ=255)
                      gTCTCCTTTCGCCGCTTGATCGTTACACAGCCATTTCTAATAGACCGTGCCCGTGCTGTGCTGCTCCCGGCCGTGGTTTAATAATTTGCTTACTAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGATATGAGGCCGGCACCGCCtcaa                                                                                                                               <  6:80741/149‑1 (MQ=255)
                       tctcCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGACCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCtcaag                                                                                                                              >  6:126575/1‑149 (MQ=255)
                               cgccgcTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTTAAGAATTTGCTTACCAATACGTGCCGGCCTCTTCGCGGGTGCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGc                                                                                                                       <  7:205933/148‑1 (MQ=255)
                                       gATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGATAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCAc                                                                                                               <  4:216908/148‑1 (MQ=255)
                                       gATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCAcc                                                                                                              >  2:63060/1‑149 (MQ=255)
                                        aTCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAgg                                                                                                                                              <  6:208205/116‑1 (MQ=255)
                                        aTCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAgg                                                                                                                                              >  5:208205/1‑116 (MQ=255)
                                          cGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGATTCTTAACCGCa                                                                                                           >  3:219166/1‑149 (MQ=255)
                                          cGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCAACGCCTCAAGTCAAGGCTTCTTCAACGCa                                                                                                           >  3:223578/1‑149 (MQ=255)
                                           gTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCa                                                                                                           <  3:253973/148‑1 (MQ=255)
                                               cacaGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCtt                                                                                                      <  5:133902/149‑1 (MQ=255)
                                                acaGCCAAGTCTAATACACCTTCCCCGAGCTGTGCTGCTCCCGGCCGTGGTTCAACAATTTGCTTACCAATAAGTGCCGCCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTc                                                                                                     <  4:82996/149‑1 (MQ=255)
                                                        gTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTc                                                                                              <  5:154467/148‑1 (MQ=255)
                                                        gTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTc                                                                                              >  6:154467/1‑148 (MQ=255)
                                                           tAATAGACCGTGACCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCtt                                                                                          <  5:126575/149‑1 (MQ=255)
                                                                 aCCGTGCCCGAGCTGTGCTGCTCCCGGCTGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACctg                                                                                    >  7:230027/1‑149 (MQ=255)
                                                                   cGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGc                                                                                                                       >  3:157858/1‑112 (MQ=255)
                                                                     tGCCTGAGTTGTGCTGCTCCCGGCGGTGGTGTATTATTTTTATTACTATTAAGTGCCGGCGTTTTGGGGGGTTCACCCTATAATTGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCtgctgc                                                                                <  4:223578/149‑1 (MQ=255)
                                                                        ccGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGcgcc                                                                             >  2:266129/1‑149 (MQ=255)
                                                                          gAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGACTCTTAGCGGGTTCACACGAGAAGAGTCCGGCACCGCCtcaagtcaag                                                                                                                         >  6:321409/1‑103 (MQ=255)
                                                                          gAGCTGTGCTGCGCCCGGCCGTGGTTGAATAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGTCCGGCACCGCCtcaagtcaag                                                                                                                         <  5:321409/103‑1 (MQ=255)
                                                                                  ctgctCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGa                                                                   <  1:63060/149‑1 (MQ=255)
                                                                                      tCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTc                                                               >  8:251938/1‑149 (MQ=255)
                                                                                              gTGGTTGAAGAATTTGCTTACGTATAAGTGCCGGACTATTCGCGGGTTCACCAGAGAAGAGGCCGGCACCGCCACAAGTCCAGGCTTATTCACCGCAGCGGTCACATAGCGCTTGACCTGCTGCGCCGCGCGTTTGAGCTCCTgcgagt                                                       >  7:55469/1‑146 (MQ=255)
                                                                                                    gaagaaTTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCgccttgcctt                                                 >  4:114558/1‑149 (MQ=255)
                                                                                                    gaagaaTTTGCTTACGAATAAGTGCCGGCCTCTTCGAGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCATCACATTTCCCTTGACATACTGCGCCTCGCCTTTGAGCTACTGCgacttgcat                                                  >  4:104333/1‑146 (MQ=255)
                                                                                                     aagaaTTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCAGCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCAcc                                                                                                              >  1:43355/1‑87 (MQ=255)
                                                                                                     aagaaTTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCAGCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCAcc                                                                                                              <  2:43355/87‑1 (MQ=255)
                                                                                                     aagaaTTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTc                                                >  4:239754/1‑149 (MQ=255)
                                                                                                      atattttGGTTACCAATACGTCCCGGCCGCTTCGCGGGTGCCCCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCg                                               <  8:230027/145‑1 (MQ=255)
                                                                                                       gaaTTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCgg                                              <  6:183846/149‑1 (MQ=255)
                                                                                                               ttACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTATTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGc                                      >  6:245563/1‑149 (MQ=255)
                                                                                                                tACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCAACCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCt                                     >  3:155920/1‑149 (MQ=255)
                                                                                                                 aCGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCtt                                    >  5:196116/1‑149 (MQ=255)
                                                                                                                  ctaataatTGCCGGCCTCTTCGCGGTTTCACCCGATAAGAGGCCGGCACCGCCTCGAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCttt                                   <  3:68798/147‑1 (MQ=255)
                                                                                                                   gAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCtttt                                  >  1:191706/1‑149 (MQ=255)
                                                                                                                     aTAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCg                                <  1:211593/149‑1 (MQ=255)
                                                                                                                     aTAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCg                                >  7:91476/1‑149 (MQ=255)
                                                                                                                         gagcctgcctCTACGCTGGTACACCAGAGAATAGGCCGGCACCGCCTCCAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGCCCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGt                            <  4:262545/147‑1 (MQ=255)
                                                                                                                         gTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGt                            >  2:39721/1‑149 (MQ=255)
                                                                                                                         gTGCCGGCAGATTCGCGGTTGCACACTCGAAGAGGCCGGCACCGGCTCCAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGt                            <  4:267389/149‑1 (MQ=255)
                                                                                                                            ccGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCgg                         >  3:211058/1‑149 (MQ=255)
                                                                                                                             cGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGt                        >  1:100159/1‑149 (MQ=255)
                                                                                                                              ggCCTCTTCTCGGGTGAACCCGCGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGt                        >  4:69454/1‑148 (MQ=255)
                                                                                                                               gCCTCTTCTCGGGTGAACCCGCGAAGAGGCCGGCACCGCCTCAAGTCACGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCa                      >  1:204868/1‑149 (MQ=39)
                                                                                                                               gCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCa                      >  5:339249/1‑149 (MQ=255)
                                                                                                                                      ctcgGGTGAACCCGCGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTgc                                                           <  8:90218/103‑1 (MQ=39)
                                                                                                                                      ctcgGGTGAACCCGCGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTgc                                                           >  7:90218/3‑105 (MQ=39)
                                                                                                                                      ctcgGGTGAACCCGCGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGc                                             <  6:72550/117‑1 (MQ=255)
                                                                                                                                      cgcgGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCACCtt                  <  3:104333/146‑1 (MQ=255)
                                                                                                                                               aCCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTAACCGCATCCTTAACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCACCTTGCCAACACCCTg      >  2:16041/1‑149 (MQ=255)
                                                                                                                                                 ccGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTc                                 <  1:299187/120‑1 (MQ=255)
                                                                                                                                                 ccGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTc                                 >  2:299187/1‑120 (MQ=255)
                                                                                                                                                 ccGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCACCTTGCCAACACCCTgct    >  4:142282/1‑147 (MQ=255)
                                                                                                                                                 ccGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTAACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCACCTTGCCAACACCCTGtt    >  8:150973/1‑149 (MQ=255)
                                                                                                                                                   gagaAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCACCTTGCCAACACCCTGTTTc  <  6:339249/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCAGGTCGGGGAAAATCATAGCGTCTCCTTTCGCCGCTTGATCGTTACACAGGCAAGTCTAATAGACCGTGCCCGAGCTGTGCTGCTCCCGGCCGTGGTTGAAGAATTTGCTTACGAATAAGTGCCGGCCTCTTCGCGGGTTCACCCGAGAAGAGGCCGGCACCGCCTCAAGTCAAGGCTTCTTCACCGCATCCTTCACATTTCCCTTGACCTGCTGCGCCTCGCCTTTGAGCTCCTGCGCCTTGCCTTCGGCCCGCAGCTTTTCGTTGTCGGTCACCTTGCCAACACCCTGTTTC  >  NC_002947/5181924‑5182219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑