Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,237,541	T→C	11.3%	M1M (TTG→CTG) †‡	PP_4614 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,237,541	0	T	C	11.3%	158.7 / 8.1	62	M1M (TTG→CTG) †‡	PP_4614	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (27/27);  new base C (2/5);  total (30/32)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCCAGTTCGCTGGACCAGGCAGTCGACGGTGCCGCCACCAAGCTCAATCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCAATGCAAAACGCCCGGCCTTGAGCCGGGCGTTTTC‑GTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTCGAACGAATCAGTGCTGCATTCGCCCCCGAATACCGG  >  NC_002947/5237394‑5237687                                                                                                                                                    |                                                                                                                                                    cGCCAGTTCGCTGGACCAGGCAGTCGACGGTGCCGCCACCAAGCTCAATCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCAATGCAAAACGCCCGGCCtt                                                                                                                                                    >  6:285324/1‑149 (MQ=255)       ttagCTGGACCAGGCAGTCGACGGAGCCCCCACCAAGCTCAATCAAGCGCTGGAGCACTTCTACGCCAAGCTGCGCAGCAAGCGCGGCGCCTACGAACTGAGAGACCCTGACGCCTGATCGGCAATGCAAAACGCCCGGCCTTGAGCCg                                                                                                                                              >  4:32056/4‑149 (MQ=255)         cGCTGGACCAGGCAGTCGACGGTGCCGCCACCAAGCTCAATCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCAATGCAAAACGCCCGGCCTTGAGCCgg                                                                                                                                             <  5:285324/148‑1 (MQ=255)            tGGACCAGGCAGTCGACGGTGCCGCCACCAAGCTCAATCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCAATGCAAAACGCCCGGCCTTGAGCCGGGCGt                                                                                                                                         >  1:85536/1‑149 (MQ=255)              gACCAGGCAGTCGACGGTGCCGCCACCAAGCTCAATCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCAATGCAAAACGCCCGGCTCAAGGCCGGGCGtt                                                                                                                                        <  3:347488/148‑1 (MQ=255)              gACCAGGCAGTCGACGGTGCCGCCACCAAGCTCAATCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCAATGCAAAACGCCCGGCTCAAGGCCGGGCGtt                                                                                                                                        <  8:27904/148‑1 (MQ=255)              gACCAGGCAGTCGACGGTGCCGCCACCAAGCTCAATCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCAATGCAAAACGCCCGGCCTTGAGCCGGGCGttt                                                                                                                                       <  8:118818/149‑1 (MQ=255)              gACCAGGCAGTCGACGGTGCCGCCACCAAGCTCAATCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCAATGCAAAACGCCCGGCCTTGAGCCGGGCGttt                                                                                                                                       >  5:210738/1‑149 (MQ=255)              gACCAGGCAGTCGACGGTGCCGCCACCAAGCTCAATCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCAATGCAAAACGCCCGGCCTTGAGCCGGGCGt                                                                                                                                         <  2:89220/147‑1 (MQ=255)               accctgCAGTCGACGGGGCCGCCACCAAGCTCAATCACGCGCTGGAGCACTTCAACCGCAAGCTGCGCAGCAAGCGCTGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCAATGCAAAACGCCCGGCTCAAGGCCGGGCGtttt                                                                                                                                      <  8:68052/144‑1 (MQ=255)               aCCAGGCAGTCGACGGTGCCGCCACCAAGCTCAATCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCAATGCAAAACGCCCGGCCTTGAGCCGGGCGtttt                                                                                                                                      >  7:37767/1‑149 (MQ=255)                  aGGCAGTCGACGGTGCCGCCACCAAGCTCAATCACGCGCTGTAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCTCCTGGGAACTGCGTGACCCGGACGCCTGATCGGCAATGCAAAACGCCCGGCCTTGAGCCGGGCGTTTTC‑Gt                                                                                                                                  <  7:122452/149‑1 (MQ=255)                      aGTCGACGGTGCCGCCACCAAGCTCAATCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCAATGCAAAACGCCCGGCCTTGAGCCGGGCGTTTTC‑GTTtgt                                                                                                                              >  5:47045/1‑149 (MQ=255)                        tCGACGGTGCCGCCACCAAGCTCAATCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCAATGCAAAACGCCCGGCCTTGAGCCGGGCGTTTTC‑GTTTGTGc                                                                                                                            <  2:246113/149‑1 (MQ=255)                        tCGACGGTGCCGCCACCAAGCTCAATCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCAATGCAAAACGCCCGGCCTTGAGCCGGGCGTTTTC‑GTTTGTGc                                                                                                                            <  8:37767/149‑1 (MQ=255)                                 ccgccACCAAGCTCAATCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCAATGCAAAACGCCCGGCCTTGAGCCGGGCGTTTTC‑GTTTGTGCCGATCAATg                                                                                                                   >  1:40335/1‑149 (MQ=255)                                  cgccACCAAGCTCAATCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCAATGCAAAACGCCCGGCCTTGAGCCGGGCGTTTTC‑GTTTGTGCCGATCAATgg                                                                                                                  <  7:21453/149‑1 (MQ=255)                                    ccaccaAGCTCAATCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCAATGCAAAACGCCCGGCCTTGAGCCGGGCGTTTTC‑GTTTGTGCCGATCAATGgcg                                                                                                                <  2:85536/149‑1 (MQ=255)                                      accaAGCTCAATCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCAATGCAAAACGCCCGGCCTTGAGCCGGGCGTTTTC‑GTTTGTGCCGATCAAt                                                                                                                    >  6:41244/1‑143 (MQ=255)                                      accaAGCTCAATCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCAATGCAAAACGCCCGGCCTTGAGCCGGGCGTTTTC‑GTTTGTGCCGATCAAt                                                                                                                    <  5:41244/143‑1 (MQ=255)                                                   aCGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCAATGCAAAACGCCCGGCCTTGAGCCGGGCGTTTTC‑GTTTGTGCCGATCAATGGCGCTGAACCAACATGc                                                                                                  >  2:358630/1‑148 (MQ=255)                                                            aGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCAATGCAAAACGCCCGGCCTTGAGCCGGGCGTTTTC‑GTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCgg                                                                                        <  4:20764/149‑1 (MQ=255)                                                            aGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCAATGCAAAACGCCCGGCCTTGAGCCGGGCGTTTTC‑GTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCgg                                                                                        <  3:206092/149‑1 (MQ=255)                                                                cTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCAATGCAAAACGCCCGGCCTTGAGCCGGGCGTTTTC‑GTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCaa                                                                                    >  2:76723/1‑149 (MQ=255)                                                                 ttCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCAATGCAAAACGCCCGGCCTTGAGCCGGGCGTTTTC‑GTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAAc                                                                                   <  1:76723/149‑1 (MQ=255)                                                                 ttCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCAATGCAAAACGCCCGGCCTTGAGCCGGGCGTTTTC‑GTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAAc                                                                                   >  6:362207/1‑149 (MQ=255)                                                                   cTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCAATGCAAAACGCCCGGCCTTGAGCCGGGCGTTTTC‑GTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAAc                                                                                   <  5:362207/147‑1 (MQ=255)                                                                   atACGGCAAGGTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGTCAATGCAAAACGCCCGGCCTTGAGCCGGGCGTTTTC‑GTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAAcc                                                                                  <  3:32056/147‑1 (MQ=255)                                                                       ggCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCAATGCAAAACGCCCGGCCTTGAGCCGGGCGTTTTC‑GTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTg                                                                              >  3:14533/1‑148 (MQ=255)                                                                         cAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCTGACGCCTGATCGGCAATGCAAAACGCCCGGCCTTGAGCCGGGCGTTTTC‑GTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCAt                                                                           <  7:12197/149‑1 (MQ=255)                                                                          aaGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCAATGCAAAACGCCCGGCCTTGAGCCGGGCGTTTTC‑GTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCAtc                                                                          <  2:40335/149‑1 (MQ=255)                                                                           aGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCAATGCAAAACGCCCGGCCTTGAGCCGGGCGTTTTC‑GTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCAtca                                                                         <  4:269046/149‑1 (MQ=255)                                                                             cTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCAATGCAAAACGCCCGGCCTTGAGCCGGGCGTTTTC‑GTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCAtcact                                                                       <  3:254319/149‑1 (MQ=255)                                                                              tGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCAATGCAAAACGCCCGGCCTTGAGCCGGGCGTTTTC‑GTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCAtcact                                                                       >  2:133440/1‑148 (MQ=255)                                                                                  cagcaAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCAATGCAAAACGCCCGGCCTTGAGCCGGGCGTTTTC‑GTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCg                                                                  >  2:56244/1‑149 (MQ=255)                                                                                     caAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCAATGCAAAACGCCCGGCCTTGAGCCGGGCGTTTTC‑GTTTGTGCCGATCAATGGGGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCg                                                                  >  7:66913/1‑146 (MQ=255)                                                                                                ccTTGGAACTGAGTGACCCGGACGCCTGATCGGCAATGCAAAACGCCCGGCCTTGAGCCGGGCGTTTTC‑GTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCa                                                    <  3:282761/149‑1 (MQ=255)                                                                                                               aCCCGGACGCCTGATCGGCAATGCAAAACGCCCGGCCTTGAGCCGGGCGTTTTC‑GTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTc                                      >  4:204261/1‑148 (MQ=255)                                                                                                               aCCCGGACGCCTGATCGGCAATGCAAAACGCCCGGCCATGAGCCGGGCGTTTTC‑GTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTc                                      >  1:176439/1‑148 (MQ=255)                                                                                                                          tGATCGGCAATGCAAAACGCCCGGCCTTGAGCCGGGCGTTTTC‑GTTTGTGc                                                                                                                            >  3:93486/1‑51 (MQ=255)                                                                                                                          tGATCGGCAATGCAAAACGCCCGGCCTTGAGCCGGGCGTTTTC‑GTTTGTGc                                                                                                                            <  4:93486/51‑1 (MQ=255)                                                                                                                          tGATCGGCAATGCAAAACGCCCGGCCTTGAGCCGGGCGTTTTC‑GTTTGTGCCGATCAATGGCGCTGAGCCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCAGTCGAACGCATCAGt                          >  8:237800/1‑149 (MQ=255)                                                                                                                          tGATCGGCAATGCAAAACGCCCGGCCTTGAGCCGGGCGTTTTC‑GTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTCGAACGAATAAGt                          >  5:232565/1‑149 (MQ=255)                                                                                                                             tCGGCAATGCAAAACGCCCGGCCTTGAGCCGGGCGTTTTC‑GTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTCGAACGAATCAGtgct                       >  3:112518/1‑149 (MQ=255)                                                                                                                                acaaacgaaaaCGCCCGGCTCACGGCCGGGCGTTTTC‑GTTTGTGCCGATCAATGGCGCTCAACCAACATGCGAACGTCCGGGCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTCGAACGAATCAGtgct                       <  6:337133/139‑1 (MQ=255)                                                                                                                                acaaacgaaaaCGCCCGGCTCAAGGCCGGGCGGTTTC‑GGGTGAGCCGATGAATGGCGCTCAACCAACATGCGAACGTCCGGTGCACCTCTGCCTACCTCACCGCAGACGCAAGACCATGAACAACCCATTCGAAGGAATCAGtgct                       >  5:337133/8‑146 (MQ=255)                                                                                                                                 cAATGCAAAACGCCCGGCCTTGAGCCGGGCGTTTTC‑GTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTCGAACGAATCAGTGCTGCAt                   <  8:66913/149‑1 (MQ=255)                                                                                                                                  aaTGCAAAACGCCCGGCCTTGAGCCGGGCGTTTTCGGTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTCGAACGAATCAGTGCTGCAt                   >  1:53498/1‑149 (MQ=255)                                                                                                                                  aaTGCAAAACGCCCGGCCTTGAGCCGGGCGTTTTC‑GTTTGTGCCGATGAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTCGAACGAATCAGTGCTGCAtt                  >  6:6318/1‑149 (MQ=255)                                                                                                                                  aaTGCAAAACGCCCGGCCTTGAGCCGGGCGTTATC‑GTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTCGAACGAATCAGTGCTGCAtt                  >  1:225184/1‑149 (MQ=255)                                                                                                                                   aTGCAAAACGCCCGGCCTTGAGCCGGGCGTTTTC‑GTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTCGAACGAATCAGTGCTGCATTc                 <  1:133440/149‑1 (MQ=255)                                                                                                                                   aTGCAAAACGCCCGGCCTTGAGCCGGGCGTTTTC‑GTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTCGAACGAATCAGTGCTGCATTc                 <  2:53498/149‑1 (MQ=255)                                                                                                                                      cAAAACGCCCGGCCTTGAGCCGGGCGTTTTC‑GTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTCGAACGAATCAGTGCTGCATTCGcc              <  2:225184/149‑1 (MQ=255)                                                                                                                                      cAAAACGCCCGGCCTTGAGCCGGGCGTTTTC‑GTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTCGAACGAATCAGTGCTGCATTCGcc              >  4:138909/1‑149 (MQ=255)                                                                                                                                        aaaCGCCCGGCTCAAGGCCGGGCGTTTTC‑GTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTc                                      <  5:357148/123‑1 (MQ=255)                                                                                                                                        aaaCGCCCGGCTCAAGGCCGGGCGTTTTC‑GTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTc                                      >  6:357148/1‑123 (MQ=255)                                                                                                                                            gCCCGGCCTTGAGCCGGGCGTTTTC‑GTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTCGAACGAATCAGtgctg                      <  2:280757/135‑1 (MQ=255)                                                                                                                                            gCCCGGCCTTGAGCCGGGCGTTTTC‑GTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTCGAACGAATCAGTGCTGCATTCGCCCCCGaa        >  8:166521/1‑149 (MQ=255)                                                                                                                                            gCCCGGCCTTGAGCCGGGCGTTTTC‑GTTTGTGCCGATCAATGGCGCTGAACCAACAGGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTCGAACGAATCAGtgctg                      >  1:280757/1‑135 (MQ=255)                                                                                                                                               cGGCCTTGAGCCGGGCGTTTTC‑GTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTCGAACGAATCAGTGCTGCATTCGCCCCCGAATAc     >  5:203643/1‑149 (MQ=255)                                                                                                                                               cGGCCTTGAGCCGGGCGTTTTC‑GTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTCGAACGAATCAGTGCTGCATTCGCCCCCGAATAc     >  6:129695/1‑149 (MQ=255)                                                                                                                                                ggCCTTGAGCCGGGCGTTTTC‑GTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTCGAACGAATCAGTGCTGCATTCGCCCCCGAATAcc    <  1:358630/149‑1 (MQ=255)                                                                                                                                                  ccTTGAGCCGGGCGTTTTC‑GTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTCGAACGAATCAGTGCTGCATTCGCCCCCGAATACCgg  <  4:14533/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    CGCCAGTTCGCTGGACCAGGCAGTCGACGGTGCCGCCACCAAGCTCAATCACGCGCTGGAGCACTTCTACGGCAAGCTGCGCAGCAAGCGCGGCGCCTTGGAACTGAGTGACCCGGACGCCTGATCGGCAATGCAAAACGCCCGGCCTTGAGCCGGGCGTTTTC‑GTTTGTGCCGATCAATGGCGCTGAACCAACATGCGAACGTCCGGTCAACCTCTGCATCACTCACCGCAGAAGCAAGACCATGAACAACCCATTCGAACGAATCAGTGCTGCATTCGCCCCCGAATACCGG  >  NC_002947/5237394‑5237687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑